IN2014DN06660A - - Google Patents

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Publication number
IN2014DN06660A
IN2014DN06660A IN6660DEN2014A IN2014DN06660A IN 2014DN06660 A IN2014DN06660 A IN 2014DN06660A IN 6660DEN2014 A IN6660DEN2014 A IN 6660DEN2014A IN 2014DN06660 A IN2014DN06660 A IN 2014DN06660A
Authority
IN
India
Prior art keywords
lysozyme
present disclosure
proteins
polypeptides
express
Prior art date
Application number
Inventor
Stefan Haertle
Sebastian Jaeger
Daniela Daubert
Original Assignee
Morphosys Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Morphosys Ag filed Critical Morphosys Ag
Publication of IN2014DN06660A publication Critical patent/IN2014DN06660A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present disclosure provides a method to express and purify polypeptides and proteins. In the present disclosure the use of lysozyme as a fusion partner is disclosed. Furthermore purification methods to isolate lysozyme tagged polypeptides and proteins via lysozyme specific antibodies are described. More specifically the present disclosure provides a method to express and purify monomeric polypeptides and proteins by using lysozyme as a tag.
IN6660DEN2014 2012-01-23 2013-01-23 IN2014DN06660A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261589408P 2012-01-23 2012-01-23
EP12152095 2012-01-23
PCT/EP2013/051181 WO2013110627A1 (en) 2012-01-23 2013-01-23 Use of lysozyme as a tag

Publications (1)

Publication Number Publication Date
IN2014DN06660A true IN2014DN06660A (en) 2015-05-22

Family

ID=48872896

Family Applications (1)

Application Number Title Priority Date Filing Date
IN6660DEN2014 IN2014DN06660A (en) 2012-01-23 2013-01-23

Country Status (11)

Country Link
US (1) US9689014B2 (en)
EP (1) EP2807179B1 (en)
JP (1) JP6215231B2 (en)
KR (1) KR20140122729A (en)
CN (1) CN104066745B (en)
AU (1) AU2013211651C1 (en)
CA (1) CA2860916A1 (en)
ES (1) ES2582802T3 (en)
HK (1) HK1202298A1 (en)
IN (1) IN2014DN06660A (en)
WO (1) WO2013110627A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105524942A (en) * 2016-02-05 2016-04-27 广州赛莱拉干细胞科技股份有限公司 Expression vector and expression method of insulin-like growth factor binding protein 3 (IGFBP3)
US10986850B2 (en) 2016-12-21 2021-04-27 Novozymes A/S Polypeptides having lysozyme activity, polynucleotides encoding same and uses and compositions thereof
CN110139666B (en) 2016-12-21 2024-07-05 诺维信公司 Polypeptides having lysozyme activity, polynucleotides encoding same, uses and compositions thereof
CN107794254A (en) * 2017-09-30 2018-03-13 昆明理工大学 The method for quickly purifying of recombinant high temperature lyases
CN116568792A (en) * 2020-12-16 2023-08-08 丹尼斯科美国公司 Enzymes and enzyme compositions for cleaning
WO2023168234A1 (en) * 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2459701A (en) 1945-07-16 1949-01-18 Harper Esther Pardee Mechanical instrument holder
US4355022A (en) 1981-07-01 1982-10-19 Interon, Inc. Method of dental treatment
JP2761881B2 (en) * 1988-03-10 1998-06-04 チッソ株式会社 An antibody-immobilized carrier for affinity chromatography
DE69024323T2 (en) 1989-10-27 1996-10-17 Genencor Int Antimicrobial method and formulation using Type II endoglycosidase and antimicrobial agent
BR9811984A (en) 1997-08-22 2000-09-05 Siemens Ag Process for regulating the stroke of an internal combustion machine
NL1013308C2 (en) 1998-10-15 2000-06-30 Dsm Nv Antimicrobial enzymes in animal feed.
EP1194570A1 (en) 1999-06-23 2002-04-10 PPL Therapeutics (Scotland) Limited Fusion proteins incorporating lysozyme
EP1470236B1 (en) 2002-01-07 2007-12-12 Lifesensors, Inc. Methods and compositions for protein expression and purification
DE10237317B4 (en) 2002-08-15 2010-04-08 3M Espe Ag Enzyme-containing composition, process for their preparation and their use
CA2404356A1 (en) 2002-09-18 2004-03-18 Canadian Inovatech Inc. Gram-positive antibacterial composition and method for use
US7428273B2 (en) 2003-09-18 2008-09-23 Promptu Systems Corporation Method and apparatus for efficient preamble detection in digital data receivers
CN1922314B (en) 2004-02-25 2010-05-05 诺维信公司 Fungall cell wall degrading enzyme
KR20100016416A (en) 2007-04-10 2010-02-12 새인트 시메온 엘디에이 Novel compositions containing lysozyme and c-1/c-4 polysaccharides and use thereof in oral care, cosmetology and dermatology, contraception, urology and gynecology
AU2008311869B2 (en) * 2007-10-17 2013-11-21 The Board Of Trustees Of The Leland Stanford Junior University Method and composition for crystallizing G protein-coupled receptors
CN101817883B (en) * 2010-03-12 2012-06-13 广东药学院 Housefly cecropin-human lysozyme fusion protein, and preparation method and application thereof

Also Published As

Publication number Publication date
ES2582802T3 (en) 2016-09-15
US20140349343A1 (en) 2014-11-27
WO2013110627A1 (en) 2013-08-01
CA2860916A1 (en) 2013-08-01
CN104066745A (en) 2014-09-24
KR20140122729A (en) 2014-10-20
CN104066745B (en) 2017-04-12
JP6215231B2 (en) 2017-10-18
HK1202298A1 (en) 2015-09-25
EP2807179B1 (en) 2016-04-20
AU2013211651A1 (en) 2014-07-24
JP2015504683A (en) 2015-02-16
AU2013211651B2 (en) 2017-03-02
US9689014B2 (en) 2017-06-27
AU2013211651C1 (en) 2017-10-12
EP2807179A1 (en) 2014-12-03

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