IN2014DN02837A - - Google Patents

Info

Publication number
IN2014DN02837A
IN2014DN02837A IN2837DEN2014A IN2014DN02837A IN 2014DN02837 A IN2014DN02837 A IN 2014DN02837A IN 2837DEN2014 A IN2837DEN2014 A IN 2837DEN2014A IN 2014DN02837 A IN2014DN02837 A IN 2014DN02837A
Authority
IN
India
Prior art keywords
polysaccharide
pap
paps
ost
enzyme
Prior art date
Application number
Inventor
Jian Liu
Robert J Linhardt
Fikri Y Avci
Eva M Munoz
Jinghua Chen
Original Assignee
Univ North Carolina
Rensselaer Polytech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina, Rensselaer Polytech Inst filed Critical Univ North Carolina
Publication of IN2014DN02837A publication Critical patent/IN2014DN02837A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/64—Preparation of S-glycosides, e.g. lincomycin

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of sulfating a polysaccharide is provided. The method can include providing a reaction mixture comprising at least one 0-sulfotransferase (OST) enzyme and 3'- phosphoadenosine 5'-phosphosulfate (PAPS); incubating a polysaccharide substrate with the reaction mixture, wherein production of the sulfated polysaccharide from the polysaccharide substrate is catalyzed by the OST enzyme with a conversion of the PAPS to adenosine 3',5'-diphosphate (PAP); and providing a reaction condition which modifies PAP to reduce an inhibitory effect of PAP on the polysaccharide sulfation.
IN2837DEN2014 2005-05-12 2006-05-12 IN2014DN02837A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68039205P 2005-05-12 2005-05-12
PCT/US2006/018778 WO2006124801A2 (en) 2005-05-12 2006-05-12 Enzymatic synthesis of sulfated polysaccharides

Publications (1)

Publication Number Publication Date
IN2014DN02837A true IN2014DN02837A (en) 2015-07-10

Family

ID=37431995

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2837DEN2014 IN2014DN02837A (en) 2005-05-12 2006-05-12

Country Status (4)

Country Link
US (2) US20090197308A1 (en)
EP (1) EP1885865A4 (en)
IN (1) IN2014DN02837A (en)
WO (1) WO2006124801A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009014715A2 (en) * 2007-07-23 2009-01-29 The University Of North Carolina At Chapel Hill Enzymatic synthesis of sulfated polysaccharides without iduronic acid residues
CN101591401A (en) * 2009-02-27 2009-12-02 江南大学 A kind of method for preparing low molecular weight heparin with high activity
WO2011109469A1 (en) 2010-03-03 2011-09-09 Neocutis Sa Compositions and methods for treatment of skin diseases and disorders using antimicrobial peptide sequestering compounds
EP2655421B1 (en) 2010-12-23 2022-02-09 The University of North Carolina At Chapel Hill Chemoenzymatic synthesis of structurally homogeneous ultra-low molecular weight heparins
WO2013149161A1 (en) * 2012-03-30 2013-10-03 Deangelis Paul L High molecular weight heparosan polymers and methods of production and use thereof
JP6581509B2 (en) 2013-02-28 2019-09-25 ティーウィノット テクノロジーズ リミテッド Chemical engineering process and apparatus for synthesizing compounds
US9951149B2 (en) 2013-06-17 2018-04-24 The University Of North Carolina At Chapel Hill Reversible heparin molecules and methods of making and using the same
BR112017003966A2 (en) 2014-08-25 2018-10-23 Full Spectrum Laboratories Ltd apparatus and methods for the simultaneous production of cannabinoid compounds
CN105483187A (en) * 2015-12-09 2016-04-13 中国药科大学 Anti-tumor effect of sulfated polysaccharide
CN108699580B (en) 2015-12-28 2022-09-23 味之素株式会社 Heparan sulphate with a high 3-O-sulphation ratio in the glucosamine residue
TWI769176B (en) * 2016-09-07 2022-07-01 瑞瑟勒綜合技術協會 Biosynthetic heparin
CN106755205A (en) * 2016-11-14 2017-05-31 江南大学 A kind of method that enzyme process prepares chondroitin sulfate
JP7088026B2 (en) 2017-01-19 2022-06-21 味の素株式会社 Method for producing a heparosan compound in which a hexuronic acid residue is isomerized
JP7330893B2 (en) * 2017-03-10 2023-08-22 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル Short-acting heparin-based antiaggregant compounds and methods
WO2019010216A1 (en) 2017-07-03 2019-01-10 The University Of North Carolina At Chapel Hill Enzymatic synthesis of homogeneous chondroitin sulfate oligosaccharides
CN117417912A (en) 2017-09-05 2024-01-19 味之素株式会社 2-O-sulfurylase mutants and 3-O-sulfurylase mutants and methods of use thereof
EP3691653A4 (en) 2017-11-03 2021-08-11 The University Of North Carolina At Chapel Hill Sulfated oligosaccharides having anti-inflammatory activity
WO2019246264A1 (en) 2018-06-20 2019-12-26 The University Of North Carolina At Chapel Hill Cell protective methods and compositions
HUE064469T2 (en) * 2018-07-11 2024-03-28 Ajinomoto Kk Method for enzymatic sulfurylation of alcohols and amines using bacterium of the family enterobacteriaceae
WO2022015794A1 (en) 2020-07-14 2022-01-20 Optimvia, Llc Methods for synthesizing non-anticoagulant heparan sulfate
MX2021008479A (en) 2019-01-15 2021-10-13 Optimvia Llc Engineered aryl sulfate-dependent enzymes.
CA3130763A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
EP3997238A1 (en) 2019-07-09 2022-05-18 Optimvia, LLC Methods for synthesizing anticoagulant polysaccharides
WO2021199445A1 (en) * 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing sulfated polysaccharide and method for producing paps
WO2021199444A1 (en) 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing heparosan and bacterium of genus escherichia having heparosan-producing ability

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3818676B2 (en) * 1994-07-22 2006-09-06 生化学工業株式会社 Heparan sulfate 6-O-sulfotransferase
JP3672359B2 (en) * 1995-07-24 2005-07-20 生化学工業株式会社 Heparan sulfate 2-O-sulfotransferase
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US6861254B1 (en) * 1997-10-24 2005-03-01 Massachusetts Institute Of Technology Heparan sulfate D-glucosaminyl 3-O-sulfotransferases, and uses therefor
US6255088B1 (en) * 1999-05-11 2001-07-03 The Scripps Research Institute Enzymatic sulfation of biomolecules
JP2005507640A (en) * 2001-03-28 2005-03-24 マサチューセッツ・インスティチュート・オブ・テクノロジー Method for 6-O sulfation of glucosaminyl and 6-O sulfated polysaccharide preparation
US7531338B2 (en) * 2002-07-05 2009-05-12 The University Of North Carolina At Chapel Hill Purified and isolated heparan sulfate 3-O-sulfotransferase isoform 5 nucleic acids and polypeptides and therapeutic and screening methods using same
WO2004017910A2 (en) * 2002-08-23 2004-03-04 Neose Technologies, Inc. Total synthesis of heparin
US7655445B2 (en) * 2003-11-12 2010-02-02 Massachusetts Institute Of Technology Methods for synthesis of sulfated saccharides
US7619637B2 (en) * 2004-04-09 2009-11-17 Samsung Electronics Co., Ltd. Systems and methods for improved gamut mapping from one image data set to another

Also Published As

Publication number Publication date
WO2006124801A2 (en) 2006-11-23
US20090197308A1 (en) 2009-08-06
EP1885865A2 (en) 2008-02-13
US20120322114A1 (en) 2012-12-20
WO2006124801A3 (en) 2007-04-12
EP1885865A4 (en) 2010-12-22
US8771995B2 (en) 2014-07-08

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