IN2012DN03403A - - Google Patents

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Publication number
IN2012DN03403A
IN2012DN03403A IN3403DEN2012A IN2012DN03403A IN 2012DN03403 A IN2012DN03403 A IN 2012DN03403A IN 3403DEN2012 A IN3403DEN2012 A IN 3403DEN2012A IN 2012DN03403 A IN2012DN03403 A IN 2012DN03403A
Authority
IN
India
Prior art keywords
simvastatin
producing
huvastatin
materials
related compounds
Prior art date
Application number
Inventor
Yi Tang
Xue Gao
Xinkai Xie
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of IN2012DN03403A publication Critical patent/IN2012DN03403A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

Abstract

The invention disclosed herein relates to methods and materials for producing simvastatin and related compounds such as huvastatin. In particular, the disclosure teaches that variants of the LovD acyltransferase polypeptide can be engineered to exhibit properties that facilitate their use in the production of simvastatin and/or huvastatin. The materials and processes disclosed herein are designed so that fermentation facilities currently producing lovastatin can be converted to producing simvastatin and related compounds with minimal modifications.
IN3403DEN2012 2009-10-08 2010-10-08 IN2012DN03403A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24989409P 2009-10-08 2009-10-08
PCT/US2010/052038 WO2011044496A2 (en) 2009-10-08 2010-10-08 LovD MUTANTS EXHIBITING IMPROVED PROPERTIES TOWARDS SIMVASTATIN SYNTHESIS

Publications (1)

Publication Number Publication Date
IN2012DN03403A true IN2012DN03403A (en) 2015-10-23

Family

ID=43857415

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3403DEN2012 IN2012DN03403A (en) 2009-10-08 2010-10-08

Country Status (7)

Country Link
US (1) US8981056B2 (en)
EP (1) EP2486129B1 (en)
CN (1) CN102695792B (en)
IL (2) IL219112B (en)
IN (1) IN2012DN03403A (en)
SG (1) SG10201504099WA (en)
WO (1) WO2011044496A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9499803B2 (en) 2009-10-08 2016-11-22 The Regents Of The University Of California Variant LovD polypeptide
CN104342416B (en) * 2013-07-26 2020-01-17 石药集团中奇制药技术（石家庄）有限公司 Lovastatin acyltransferase comprising one or several point mutations
CN104342417A (en) * 2013-08-09 2015-02-11 南京朗恩生物科技有限公司 Aspergillus terreus-derived activity-enhanced acyltransferase mutant
CN103725727B (en) * 2014-01-06 2016-06-08 苏州汉酶生物技术有限公司 The preparation method of a kind of simvastatin ammonium salt
CN105586325A (en) * 2016-01-29 2016-05-18 重庆西南合成制药有限公司 Lovastatin acyltransferase mutant
US11749375B2 (en) 2017-09-14 2023-09-05 Lifemine Therapeutics, Inc. Human therapeutic targets and modulators thereof

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444784A (en) 1980-08-05 1984-04-24 Merck & Co., Inc. Antihypercholesterolemic compounds
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4582915A (en) 1983-10-11 1986-04-15 Merck & Co., Inc. Process for C-methylation of 2-methylbutyrates
US4820850A (en) 1987-07-10 1989-04-11 Merck & Co., Inc. Process for α-C-alkylation of the 8-acyl group on mevinolin and analogs thereof
US5420024A (en) 1993-05-11 1995-05-30 Merck & Co., Inc. Process for synthesis of acylated HMG-CoA reductase inhibitors from a lactone diol precursor using Candida cylindracea
US5712146A (en) 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
US6558942B1 (en) 1994-05-06 2003-05-06 The Leland Stanford Junior University Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
EP0726940A4 (en) 1993-11-02 2001-05-23 Merck & Co Inc Dna encoding triol polyketide synthase
US5393893A (en) 1993-11-08 1995-02-28 Apotex, Inc. Process for producing simvastatin and analogs thereof
US5763653A (en) 1997-03-13 1998-06-09 Ranbaxy Laboratories, Ltd. Key intermediates in the manufacture of simvastatin
US5763646A (en) 1997-03-13 1998-06-09 Ranbaxy Laboratories, Ltd. Process for manufacturing simvastatin from lovastatin or mevinolinic acid
NO991045L (en) 1998-03-05 1999-09-06 Synthon Bv Process for the preparation of simvastatin and / or its derivatives
US6391583B1 (en) * 1998-12-18 2002-05-21 Wisconsin Alumni Research Foundation Method of producing antihypercholesterolemic agents
DE60026563D1 (en) 1999-10-25 2006-05-04 Kosan Biosciences Inc PREPARATION OF POLYCETIDES
WO2001031035A2 (en) 1999-10-27 2001-05-03 Kosan Biosciences, Inc. Heterologous production of polyketides
PT1363516E (en) 2001-02-27 2005-11-30 Chong Kun Dang Pharm Corp AN IMPROVED PROCESS FOR THE PREPARATION OF SINVASTATIN
WO2003010324A2 (en) 2001-07-25 2003-02-06 International Centre For Genetic Engineering And Biotechnology Process for the preparation of simvastatin
KR100435142B1 (en) 2002-01-09 2004-06-09 한미약품 주식회사 Improved process for the preparation of simvastatin
CA2543348A1 (en) 2003-10-21 2005-05-06 Diversa Corporation Methods for making simvastatin and intermediates
WO2005066150A1 (en) 2004-01-02 2005-07-21 Hetero Drugs Limited A novel process for the preparation of simvastatin
EP2032715B1 (en) * 2006-05-24 2012-02-01 The Regents of the University of California Methods and materials for making simvastatin and related compounds
US20090197311A1 (en) 2006-06-20 2009-08-06 Marco Alexander Van Den Berg Method for the production of simvastatin
WO2009077523A2 (en) 2007-12-18 2009-06-25 Dsm Ip Assets B.V. Improved statin production
HUE046680T2 (en) 2009-09-30 2020-03-30 Codexis Inc Improved lov-d acyltransferase mediated acylation

Also Published As

Publication number Publication date
EP2486129A2 (en) 2012-08-15
IL266709B (en) 2020-10-29
IL219112B (en) 2019-06-30
WO2011044496A3 (en) 2011-06-03
CN102695792A (en) 2012-09-26
CN102695792B (en) 2015-02-18
EP2486129A4 (en) 2013-03-27
IL266709A (en) 2019-07-31
EP2486129B1 (en) 2016-04-13
US8981056B2 (en) 2015-03-17
SG10201504099WA (en) 2015-06-29
US20120190038A1 (en) 2012-07-26
WO2011044496A2 (en) 2011-04-14
IL219112A0 (en) 2012-06-28

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