IL186510A - Isolated truncated mammalian uricase comprising an amino acid substitution at position 46, the said truncation and amino acid substitution being relative to a naturally occurring pig uricase - Google Patents
Isolated truncated mammalian uricase comprising an amino acid substitution at position 46, the said truncation and amino acid substitution being relative to a naturally occurring pig uricaseInfo
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- IL186510A IL186510A IL186510A IL18651007A IL186510A IL 186510 A IL186510 A IL 186510A IL 186510 A IL186510 A IL 186510A IL 18651007 A IL18651007 A IL 18651007A IL 186510 A IL186510 A IL 186510A
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- C12Y107/03003—Factor-independent urate hydroxylase (1.7.3.3), i.e. uricase
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Abstract
Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.
Description
186510/2 nsrnnn v> iiittJinni ΊΙΟΝΛ w>pn ,46 mova ΛΊΟΠΙΟ Λ»3»ΟΝ nsow .»ttn pi v> ^sipa wp w An isolated truncated mammalian uricase comprising an amino acid substitution at position 46, the said truncation and amino acid substitution being relative to a naturally occurring pig uricase Savient Pharmaceuticals Inc.
C. 178398 - 1 - 186510/3 FIELD OF INVENTION
[0002] The present invention relates to genetically modified proteins with uricolytic activity. More specifically, the invention relates to proteins comprising truncated urate oxidases and methods for producing them.
BACKGROUND OF THE INVENTION
[0003] The terms urate oxidase and unease are used herein interchangeably.
Urate oxidases (uncases; E.C. 1.7.3.3) are enzymes which catalyze the oxidation of uric acid to a more soluble product, allantoin, a purine metabolite that is more readily excreted. Humans do not produce enzymatically active uricase, as a result of several mutations in the gene for uricase acquired during the evolution of higher primates. Wu, X, et al, (1992) J Mol Evol 34:78-84, incorporated herein by reference in its entirety. As a consequence, in susceptible individuals, excessive concentrations of uric acid in the blood (hyperuricemia) can lead to painful arthritis (gout), disfiguring urate deposits (tophi) and renal failure. In some affected individuals, available drugs such as allopurinol (an inhibitor of uric acid synthesis) produce treatment-limiting adverse effects or do not relieve these conditions adequately. Hande, KR, et al, (1984) Am J Med 76:47-56; Fam, AG, (1990) Bailliere 's Clin Rheumatol 4:177-192, each incorporated herein by reference in its entirety. Injections of uncase can decrease hyperuricemia and hyperuricosuria, at least transiently. Since uricase is a foreign protein in humans, even the first injection of the unmodified protein from Aspergillus flavus has induced anaphylactic reactions in several percent of treated patients (Pui, C-H, et al, (1997) Leukemia 1 1 : 1813-1816, incorporated herein by reference in its entirety), and immunologic responses limit its utility for chronic or intermittent treatment. Donadio, D, et al. , (1981) Nouv Presse Med 10:71 1-712; Leaustic, M, et al., (1983) Rev Rhum Mai Osteoartic 50:553-554, each incorporated herein by reference in its entirety.
[0004] The sub-optimal performance of available treatments for hyperuricemia has been recognized for several decades. Kissel, P, et al, (1968) Nature 217:72-74, incorporated herein by reference in its entirety. Similarly, the possibility that certain groups of patients with severe gout might benefit from a safe and effective form of injectable uricase has been recognized for many years. Davis, FF, et al , (1978) in GB Broun, et al, (Eds.) Enzyme Engineering, Vol. 4 (pp. 169-173) New York, Plenum Press; Nishimura, H, et al, (1979) Enzyme 24:261-264; Nishimura, H, et al, (1981) Enzyme 26:49-53; Davis, S, et al, (1981) Lancet 2(8241):281-283; Abuchowski, A, et al , (1981) J Pharmacol Exp Ther 219:352-354; Chen, RH-L, et al , (1981) Biochim Biophys Acta 660:293-298; Chua, CC, et al , (198S) Ann Int Med 109:114-117; Greenberg, ML, et al, (1989) Anal Biochem 176:290-293, each incorporated herein by reference in its entirety. Uricases derived from animal organs are nearly insoluble in solvents that are compatible with safe administration by injection. U.S. Patent No. 3,616,231, incorporated herein by reference in its entirety. Certain uricases derived from plants or from microorganisms are more soluble in medically acceptable solvents. However, injection of the microbial enz)'mes quickly induces immunological responses that can lead to life-threatening allergic reactions or to inactivation and/or accelerated clearance of the uricase from the circulation. Donadio, et al, (1981); Leaustic, et al , (1983). Enzymes based on the deduced amino acid sequences of uricases from mammals, including pig and baboon, or from insects, such as, for example, Drosophila melanogaster or Drosophila pseudoobscura (Wallrath, LL, et al, (1990) Mol Cell Biol 10:5114-5127, incorporated herein by reference in its entirety), have not been suitable candidates for clinical use, due to problems of immunogenicity and insolubility at physiological pH.
[0005] Previously, investigators have used injected uricase to catalyze the conversion of uric acid to allantoin in vivo. See Pui, et al, (1997). This is the basis for the use in France and Italy of uricase from the fungus Aspergillus flavus (Uricozyme®) to prevent or temporarily correct the hyperuricemia associated with cytotoxic therapy for hematologic malignancies and to transiently reduce severe hyperuricemia in patients with gout. Potaux, L; et al, (1975) Nouv Presse Med 4: 1109-1 112; Legoux, R, et al, (1992) J Biol Chem 267:8565-8570; U.S. Patents 5,382,518 and 5,541,098, each incorporated herein by reference in its entirety. Because of its short circulating lifetime, Uricozyme® requires daily injections. Furthermore, it is not well suited for long-term therapy because of its immunogenicity.
[0006] Certain uricases are useful for preparing conjugates with poly(ethylene glycol) or poly(ethylene oxide) (both referred to as PEG) to produce therapeutically efficacious forms of uricase having increased protein half-life and reduced immunogenicity. U.S. Patents 4,179,337, 4,766,106, 4,847,325, and 6,576,235; U.S. Patent Application Publication US2003/0082786A1, each incorporated herein by reference in its entirety. Conjugates of uricase with polymers other than PEG have also been described. U.S. Patent 4,460,683, incorporated herein by reference in its entirety.
[0007] In nearly all of the reported attempts to PEGylate uricase (i.e. to covalently couple PEG to uricase), the PEG is attached primarily to amino groups, including the amino-terminal residue and the available lysine residues. In the uricases commonly used, the total number of lysines in each of the four identical subunits is between 25 (Aspergillus flavus (U.S. Patent 5,382.518, incorporated herein by reference in its entirety)) and 29 (pig (Wu, X, et al., (1989) Proc Natl Acad Sci USA 86:9412-9416, incorporated herein by reference in its entirety)). Some of the lysines are unavailable for PEGylation in the native conformation of the enzyme. The most common approach to reducing the immunogenicity of uricase has been to couple large numbers of strands of low molecular weight PEG. This has invariably resulted in large decreases in the enzymatic activity of the resultant conjugates.
[0008] A single intravenous injection of a preparation of Candida utilis uricase coupled to 5 kDa PEG reduced serum urate to undetectable levels in five human subjects whose average pre-injection serum urate concentration is 6.2 mg/dl, which is within the noraial range. Davis, et al, (1981). The subjects were given an additional injection four weeks later, but their responses were not reported. No antibodies to uricase were detected following the second (and last) injection, using a relatively insensitive gel diffusion assay. This reference reported no results from chronic or subchronic treatments of human patients or experimental animals.
[0009] A preparation of uricase from Arthrobacter protoformiae coupled to 5 kDa PEG was used to temporarily control hyperuricemia in a single patient with lymphoma whose pre-injection serum urate concentration is 15 mg/dL. Chua, et al., (1988). Because of the critical condition of the patient and the short duration of treatment (four injections during 14 days), it is not possible to evaluate the long-term efficacy or safety of the conjugate.
[0010] Improved protection from immune recognition is enabled by modifying each uricase subunit with 2 - 10 strands of high molecular weight PEG (>5 kD - 120 kD) Saifer, et al. (U.S. Patent 6,576,235; (1994) Adv Exp Med Biol 366:377-387, each incorporated herein by reference in its entirety). This strategy enabled retention of >75% enzymatic activity of uricase from - 5 - 186510/2 various species, following PEGylation, enhanced the circulating life of uricase, and enabled repeated injection of the enzyme without eliciting antibodies in mice and rabbits.
[0011] Hershfield and Kelly (International Patent Publication WO 00/08196; U.S.
Application No. 60/095,489 (unpublished provisional patent application to which WO 00/08196 claims priority), incorporated herein by reference in its entirety) developed means for providing recombinant uricase proteins of mammalian species with optimal numbers of PEGylation sites. They used PCR techniques to increase the number of available lysine residues at selected points on the enzyme which is designed to enable reduced recognition by the immune system, after subsequent PEGylation, while substantially retaining the enzyme's uricolytic activity. Some of their uricase proteins are truncated at the carboxy and/or amino termini. They do not provide for directing other specific genetically-induced alterations in the protein.
[0012] In this application, the term "immunogenicity" refers to the induction of an immune response by an injected preparation of PEG-modified or unmodified uricase (the antigen), while "antigenicity" refers to the reaction of an antigen with preexisting antibodies. Collectively, antigenicity and immunogenicity are referred to as "immunoreactivity." In previous studies of PEG-uricase, immunoreactivity is assessed by a variety of methods, including: 1) the reaction in vitro of PEG-uricase with preformed antibodies; 2) measurements of induced antibody synthesis; and 3) accelerated clearance rates after repeated injections.
[0013] Previous attempts to eliminate the immunogenicity of uricases from several sources by coupling various numbers of strands of PEG through various linkers have met with limited success. PEG-uricases were first disclosed by FF Davis and by Y Inada and their colleagues. Davis, et al, (1978); U.S. Patent 4,179,337; Nishimura, et al, (1979); Japanese Patents 55-99189 and 62-55079,, each incorporated herein by reference in its entirety. The conjugate disclosed in U.S. Patent 4,179,337 is synthesized by reacting uricase of unspecified origin with a 2,000-fold molar excess of 750 dalton PEG, indicating that a large number of polymer molecules is likely to have been attached to each uricase subunit. U.S. Patent 4.179,337 discloses the coupling of either PEG or polypropylene glycol) with molecular weights of 500 to 20,000 daltons, preferably about 500 to 5,000 daltons, to provide active, water-soluble, non-imniunogenic conjugates of various polypeptide hormones and enzymes including oxidoreductases, of which uricase is one of three examples. In addition, U.S. Patent 4,179,337 emphasizes the coupling of 10 to 100 polymer strands per molecule of enzyme, and the retention of at least 40% of enzymatic activity. No test results were reported for the extent of coupling of PEG to the available amino groups of uricase, the residual specific uricolytic activity, or the immunoreactivity of the conjugate.
[0014] In previous publications, significant decreases in uricolytic activity measured in vitro were caused by coupling various numbers of strands of PEG to uricase from Candida utilis. Coupling a large number of strands of 5 kDa PEG to porcine liver uricase gave similar results, as described in both the Chen publication and a symposium report by the same group. Chen, et al., (1981); Davis, et al., (1978).
[0015] In seven previous studies, the immunoreactivity of uricase is reported to be decreased by PEGylation and was eliminated in five other studies. In three of the latter five studies, the elimination of immunoreactivity is associated with profound decreases in uricolytic activity - to at most 15%, 28%, or 45% of the initial activity. Nishimura, et al., (1979) (15% activity); Chen, et al., (1981) (28% activity); Nishimura, et al., (1981) (45% activity). In the fourth report, PEG is reported to be coupled to 61% of the available lysine residues, but the residual specific activity is not stated. Abuchowski, et al., (1981). However, a research team that included two of the same scientists and used the same methods reported elsewhere that this extent of coupling left residual activity of only 23-28%. Chen, et al., (1981). The 1981 publications of Abuchowski et al., and Chen et al., indicate that to reduce the inmiunogenicity of uricase substantially, PEG must be coupled to approximately 60% of the available lysine residues. The fifth publication in which the iimminoreactivity of uricase is reported to have been eliminated does not disclose the extent of PEG coupling, the residual uricolytic activity, or the nature of the PEG-protein linkage.
Veronese, FM, et al., (1997) in JM Harris, et al., (Eds.), Poly(ethylene glycol) Chemistry and Biological Applications. ACS Symposium Series 680 (pp. 182-192) Washington, DC: American Chemical Society, incorporated herein by reference in its entirety.
[0016] Conjugation of PEG to a smaller fraction of the lysine residues in uricase reduced but did not eliminate its immunoreactivity in experimental animals. Tsuji, J, et al., (1985) Int J Immunopharmacol 7:725-730, incorporated herein by reference in its entirety (28-45% of the amino groups coupled); Yasuda, Y, et al., (1990) Chem Pharm Bull 38:2053-2056, incorporated herein by reference in its entirety (38% of the amino groups coupled). The residual uricolytic activities of the corresponding adducts ranged from <33% (Tsuji, et al.) to 60% (Yasuda, et al.) of their initial values. Tsuji, et al., synthesized PEG-uricase conjugates with 7.5 kDa and 10 kDa PEGs, in addition to 5 kDa PEG. All of the resultant conjugates are somewhat immunogenic and antigenic, while displaying markedly reduced enzymatic activities.
[0017] A PEGylated preparation of uricase from Candida utilis that is safely administered twice to each of five humans is reported to have retained only 1 1 % of its initial activity. Davis, et al., (1981). Several years later, PEG-modified uricase from Arthrobacter protoformiae was administered four times to one patient with advanced lymphoma and severe hyperuricemia. Chua, et al, (1988). While the residual activity of that enzyme preparation was not measured, Chua, et al, demonstrated the absence of anti-uricase antibodies in the patient's serum 26 days after the first PEG-uricase injection, using an enzyme-linked immunosorbent assay (ELISA).
[0018] Previous studies of PEGylated uricase show that catalytic activity is markedly depressed by coupling a sufficient number of strands of PEG to decrease its immunoreactivity substantially. Furthermore, most previous preparations of PEG-uricase are synthesized using PEG activated with cyanuric chloride, a triazine derivative (2,4,6-trichloro-l,3,5-triazine) that has been shown to introduce new antigenic determinants and to induce the formation of antibodies in rabbits. Tsuji, et al., (1985).
[0019] Japanese Patent No. 3-148298 to A Sano, et al., incorporated herein by reference in its entirety, discloses modified proteins, including uricase, derivatized with PEG having a molecular weight of 1-12 kDa that show reduced antigenicity and "improved prolonged" action, and methods of making such derivatized peptides. However, there are no disclosures regarding strand counts, enzyme assays, biological tests or the meaning of "improved prolonged." Japanese Patents 55-99189 and 62-55079, each incorporated herein by reference in its entirety, both to Y Inada, disclose uricase conjugates prepared with PEG-triazine or -n's-PEG-triazine (denoted as PEG2), respectively. See Nishimura, et al, (1979 and 1981). In the first type of conjugate, the molecular weights of the PEGs are 2 kDa and.5 kDa, while in the second, only 5 kDa PEG is used. Nishimura, et al, (1979) reported the recovery of 15% of the uricolytic activity after modification of 43% of the available lysines with linear 5 kDa PEG, while Nishimura, et al, (1981) reported the recovery of 31% or 45% of the uricolytic activity after modification of 46% or 36% of the lysines, respectively, with PEG2.
[0020] Previously studied uricase proteins were either natural or recombinant proteins. However, studies using SDS-PAGE and/or Western techniques revealed the presence of unexpected low molecular weight peptides which appear to be degradation products and increase - 9 - 186510/2 in frequency over time. The present invention is related to mutant recombinant unease proteins having truncations and enhanced structural stability.
WO 00/07629 describes naturally occurring or recombinant urate oxidase (uricase) covalently couples to poly(ethylene glycol) or poly(ethylene oxide) [both referred to as PEG], wherein an average of 2 to 10 strands of PEG are conjugated to each uricase subunit and the PEG has an average molecular weight between 5 kDa and 100 kDa; the resulting PEG-uricase conjugates are substantially non-immunogenic and retain at least 75 % of the uricolytic activity of the unmodified enzyme.
WO 00/08196 describes urate oxidase (uricase) proteins and nucleic acids encoding such proteins, and in particular, uricase proteins useful as intermediates for making modified uricase proteins with reduced immunogenicity and increased bioavailability.
SUMMARY OF THE INVENTION In one of its aspects the present invention provides an isolated truncated mammalian uricase comprising which is truncated at the amino terminus or the carboxy terminus or both the amino and carboxy termini by about 1 - 6 amino acids and further comprising an amino acid substitution at position 46, said truncation and amino acid substitution being relative to a naturally occurring pig uricase having an amino acid sequence of SEQ ID NO:l 1.
In another of its aspects the present invention provides a polyethylene glycol -uricase conjugate comprising the uricase of the invention.
In a further of its aspects the present invention provides a pharmaceutical composition comprising a conjugate of the invention.
Yet in a further of its aspects the preset invention provides the composition of the invention for use in reducing uric acid levels in a biological fluid of a subject, wherein said composition is adapted to administration to said subject. - 9a - 186510/1 In yet a further of its aspects the present invention provides an isolated uricase comprising the amino acid sequence of SEQ ID NO: 14.
In another of its aspects the present invention provides an isolated nucleic acid comprising a nucleic acid sequence which encodes a protein of the invention.
In a further of its aspects the present invention provides a nucleic acid vector comprising the nucleic acid of the invention.
In yet a further of its aspects the present invention provides a host cell comprising the vector of the invention.
Yet in a further of its aspects the present invention provides an isolated nucleic acid comprising a nucleic acid sequence encoding a uricase comprising the amino acid sequence of SEQ ID NO:7 or SEQ ID NO:8.
In a further of its aspects the present invention provides a method for producing a uricase comprising the steps of culturing a host cell of the invention under conditions such that the nucleic acid sequence is expressed by the host cell and isolating the expressed uricase.
DETAILED DESCRIPTION OF THE INVENTION
[0021] The present invention provides novel recombinant uricase proteins. In one embodiment, the proteins of the invention contemplated are truncated and have mutated amino acids relative to naturally occurring uricase proteins. In particular embodiments, the mutations are at or around the areas of amino acids 7, 46, 291, and 301. Conservative mutations anywhere in the peptide are also contemplated as a part of the invention.
[0022] The subject invention provides a mutant recombinant uricase, wherein the uricase has been truncated by 1 - 20 amino acids and retains the uricolytic activity of the naturally occurring uricase. The truncations are at or around the sequence termini such that the protein - 9b - 186510/1 may contain the ultimate amino acids. These mutations and truncations may enhance stability of the protein comprising such mutations.
[0023] In another embodiment, the present invention to provides a means for metabolizing uric acid comprising a novel recombinant unease protein having uncolytic activity. Uncolytic activity is used herein to refer to the enzymatic conversion of uric acid to allantoin.
[0024] The subject invention further provides a host cell with the capacity for producing a uricase that has been truncated by 1 - 20 amino acids, and has mutated amino acids and retains uricolytic activity.
[0025] In an embodiment, an isolated truncated mammalian uricase is provided comprising a mammalian uricase amino acid sequence truncated at the amino terminus or the carboxy terminus or both the amino and carboxy termini by about 1 - 13 amino acids and further - 10 -comprising an amino acid substitution at about position 46. In particular embodiments, the uricase comprises an amino terminal amino acid, wherein the amino terminal amino acid is alanine, glycine, proline, serine, or threonine. Also provided is a uricase wherein there is a substitution at about position 46 with threonine or alanine. In an embodiment, the uricase comprises the amino acid sequence of SEQ ID NO. 8. In an embodiment, the uricase is conjugated with a polymer to form, for example, a polyethylene glycol - uricase conjugate. In particular embodiments, polyethylene glycol - uricase conjugates comprise 2 to 12 polyethylene glycol molecules on each uricase subunit, preferably 3 to 10 polyethylene glycol molecules per uricase subunit. In particular embodiments, each polyethylene glycol molecule of the polyethylene glycol - uricase conjugate has a molecular weight between about 1 kD and 100 kD; about 1 kD and 50 kD; about 5 kD and 20 kD; or about 10 kD. Also provided are pharmaceutical compositions comprising the uiicase of the invention, including the polyethylene glycol - uricase conjugate. In an embodiment, the pharmaceutical composition is suitable for repeated administration.
[0026] Also provided is a method of reducing uric acid levels in a biological fluid of a subject in need thereof, comprising administering the pharmaceutical composition comprising the uiicase of the invention. In a particular embodiment, the biological fluid is blood.
[0027] In an embodiment, the uiicase comprises a peptide having the sequence of position 44 to position 56 of Pig-KS-ΔΝ (SEQ ID NO. 14).
[0028] In an embodiment, the uiicase protein comprises an N-tenninal methionine, hi a particular embodiment, the uricase comprises the amino acid sequence of SEQ ID NO. 7. - 1 1 -
[0029] Also provided are isolated nucleic acids comprising a nucleic acid sequence which encodes a uricase of the invention, for example, uricases having or comprising the amino acid sequences of SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 12 or SEQ ID NO. 13. In an embodiment, the isolated nucleic acid is operatively linked to a heterologous promoter, for example, the osmB promoter. Also provided are vectors comprising uricase encoding nucleic acids, and host cells comprising such vectors. In an embodiment, the nucleic acid has the sequence of SEQ ID NO. 7. Also provided is a method for producing a uricase comprising the steps of culturing such a host cell under conditions such that uricase is expressed by the host cell and isolating the expressed uricase.
BRIEF DESCRIPTION OF THE DRAWINGS
[0030] Figure 1 illustrates the structure of plasmid pOUR-P-AN-ks-l . Numbers next to restriction sites indicate nucleotide position, relative to Haell site, designated as 1. Restriction sites which are lost during cloning are marked in parenthesis.
[0031] Figure 2 depicts the DNA and the deduced amino acid sequences of Pig-KS-ΔΝ uricase (SEQ ID NO. 9 and SEQ ID NO. 7, respectively). The amino acid numbering in Figure 2 is relative to the complete pig uricase sequence. Following the initiator methionine residue, a threonine replaces aspartic acid 7 of the pig uricase sequence. The restriction sites that are used for the various steps of subcloning are indicated. The 3 ' untranslated sequence is shown in lowercase letters. The translation stop codon is indicated by an asterisk.
[0032] Figure 3 shows relative alignment of the deduced amino acid sequences of the various recombinant pig (SEQ ID NO. 1 1), PBC-ANC (SEQ ID NO. 12), and Pig-KS-ΔΝ (SEQ ID NO. 7) uricase sequences. The asterisks indicate the positions in which there are differences in amino acids in the Pig-KS-ΔΝ as compared to the published pig uricase sequence; the circles - 12 -indicate positions in which there are differences in amino acids in Pig-KS-ΔΝ as compared to PBC-ΔΝ. Dashed lines indicate deletion of amino acids.
[0033] Figure 4 depicts SDS-PAGE of pig uricase and the highly purified uricase variants produced according to Examples 1 - 3. The production date (month/ year) and the relevant lane number for each sample is indicated in the key below. The Y axis is labeled with the weights of molecular weight markers, and the top of the figure is labeled with the lane numbers. The lanes are as follows: Lane 1 - Molecular weight markers; Lane 2 - Pig KS-ΔΝ (7/98); Lane 3 - Pig (9/98); Lane 4 - Pig KS (6/99); Lane 5 - Pig KS (6/99); Lane 6 - Pig-ΔΝ (6/99); Lane 7 - Pig KS-ΔΝ (7/99); Lane 8 - Pig KS-ΔΝ (8/99).
[0034] Figure 5 depicts the pharmacokinetic profiles of PEGylated (9x10 kD) Pig-KS-ΔΝ uricase in rats following IM (intramuscular), SC (subcutaneous), and IV (intravenous) injections, as determined by monitoring enzymatic activity in blood samples. Uricase activity in plasma samples, which are collected at the indicated time points, is determined using the colorimetric assay. Activity values (mAU = milli-absorbance units) represent the rate of enzymatic reaction per 1 μΐ of plasma sample. The bioavailability (amount of drug reaching the circulation relative to an IV injection) of uricase injected was calculated from the area under the curve of the graph.
[0035] Figure 6 depicts the pharmacokinetic profiles of PEGylated (9x10 kD) Pig-KS-ΔΝ uricase in rabbits following IM (intramuscular), SC (subcutaneous), and IV (intravenous) injections, as determined by monitoring enzymatic activity in blood samples. Uricase activity in plasma samples collected at the indicated time points is deteimined using a colorimetric assay. Activity values (mAU = milli-absorbance units) represent the rate of enzymatic reaction per 1 μΐ of plasma sample. The bioavailability (amount of drug reaching the circulation relative to an IV injection) of uricase injected was calculated from the area under the curve of the graph. - 13 - 186510/2
[0036] Figure 7 depicts the pharmacokinetic profiles of PEGylated (9x 10 kD) Pig-KS-ΔΝ uricase in dogs following IM (intramuscular), SC (subcutaneous), and IV (intravenous) injections, as determined by monitoring enzymatic activity in blood samples. Uricase activity in plasma samples, which are collected at the indicated time points, is determined using the colorimetric assay. Activity values (mAU = milli-absorbance units) represent the rate of enzymatic reaction per 1 μΐ of plasma sample. The bioavailability (amount of drug reaching the circulation relative to an IV injection) of uricase injected was calculated from the area under the curve of the graph.
[0037] Figure 8 depicts the pharmacokinetic profiles of PEGylated (9x 10 kD) Pig-KS-ΔΝ uricase in pigs following IM (intramuscular), SC (subcutaneous), and IV (intravenous) injections, as determined by monitoring enzymatic activity in blood samples. Uricase activity in plasma samples, which are collected at the indicated time points, is determined using the colorimetric assay. Activity values (mAU = milli-absorbance units) represent the rate of enzymatic reaction per 1 μΐ of plasma sample. The bioavailability (amount of drug reaching the circulation relative to an IV injection) of uricase injected was calculated from the area under the curve of the graph.
[0038] Previous studies teach that when a significant reduction in the immunogenicity and/or antigenicity of uricase is achieved by PEGylation, it is invariably associated with a substantial loss of uricolytic activity. The safety, convenience and cost-effectiveness of biopharmaceuticals are all adversely impacted by decreases in their potencies and the resultant need to increase the administered dose. Thus, there is a need for a safe and effective alternative means for lowering elevated levels of uric acid in body fluids, including blood. The present invention provides a - 14 -mutant recombinant uricase, wherein the uricase has been truncated by 1 - 20 amino acids at either the amino terminus or the carboxy terminus, or both, and substantially retains uricolytic activity of the naturally occurring uricase.
[0039] Uricase, as used herein, includes individual subunits, as well as the tetramer, unless otherwise indicated.
[0040] Mutated uricase, as used herein, refers to uricase molecules having amino acids exchanged with other amino acids.
[0041] A conservative mutation, as used herein, is a mutation of one or more amino acids, at or around a position, that does not substantially alter the protein's behavior. In a preferred embodiment, the uricase comprising at least one conservative mutation has the same uricase activity as does uricase without such mutation. In alternate embodiments, the uricase comprising at least one conservative mutation has substantially the same uricase activity, within 5% of the activity, within 10% of the activity, or within 30% of the activity of uricase without such mutation.
[0042] Conservative amino acid substitution is defined as a change in the amino acid composition by way of changing amino acids of a peptide, polypeptide or protein, or fragment thereof. In particular embodiments, the uricase has one, two, three or four conservative mutations. The substitution is of amino acids with generally similar properties (e.g., acidic, basic, aromatic, size, positively or negatively charged, polar, non-polar) such that the substitutions do not substantially alter peptide, polypeptide or protein characteristics (e.g., charge, IEF, affinity, avidity, conformation, solubility) or activity. Typical substitutions that - 1 5 -may be performed for such conservative amino acid substitution may be among the groups of amino acids as follows:
[0043] glycine (G), alanine (A), valine (V), leucine (L) and isoleucine (I)
[0044] aspartic acid (D) and glutamic acid (E)
[0045] alanine (A), serine (S) and threonine (T)
[0046] histidine (H), lysine (K) and arginine (R)
[0047] asparagine (N) and glutamine (Q)
[0048] phenylalanine (F), tyrosine (Y) and tryptophan (W)
[0049] The protein having one or more conservative substitutions retains its structural stability and can catalyze a reaction even though its DNA sequence is not the same as that of the original protein.
[0050] Truncated uricase, as used herein, refers to uricase molecules having shortened primary amino acid sequences. Amongst the possible truncations are truncations at or around the amino and/or carboxy termini. Specific truncations of this type may be such that the ultimate amino acids (those of the amino and/or carboxy terminus) of the naturally occurring protein are present in the truncated protein. Amino terminal truncations may begin at position 1, 2, 3, 4, 5 or 6. Preferably, the amino terminal truncations begin at position 2, thereby leaving the amino terminal methionine. This methionine may be removed by post-translational modification. In particular embodiments, the amino terminal methionine is removed after the uricase is produced. In a particular embodiment, the methionine is removed by endogenous bacterial aminopeptidase.
[0051] A truncated uricase, with respect to the full length sequence, has one or more amino acid sequences excluded. A protein comprising a truncated uricase may include any amino acid sequence in addition to the truncated uricase sequence, but does not include a protein comprising - 16 -a uiicase sequence containing any additional sequential wild type amino acid sequence. In other words, a protein comprising a truncated uricase wherein the truncation begins at position 6 (i.e., the truncated uricase begins at position 7) does not have, immediately upstream from the truncated uricase, whatever amino acid that the wild type uricase has at position 6.
[0052] Unless otherwise indicated by specific reference to another sequence or a particular SEQ ID NO., reference to the numbered positions of the amino acids of the uricases described herein is made with respect to the numbering of the amino acids of the pig uricase sequence. The amino acid sequence of pig uricase and the numbered positions of the amino acids comprising that sequence may be found in Figure 3. As used herein, reference to amino acids or nucleic acids "from position X to position Y" means the contiguous sequence beginning at position X and ending at position Y, including the amino acids or nucleic acids at both positions X and Y.
[0053] Uricase genes and proteins have been identified in several mammalian species, for example, pig, baboon, rat, rabbit, mouse, and rhesus monkey. The sequences of various uricase proteins are described herein by reference to their public data base accession numbers, as follows: gi|50403728|sp|P25689; gi|20513634|dbj|BAB91555.1 ; gi|176610|gb|AAA35395.1 ; gi|20513654|dbj|BAB91557.1 ; gi|47523606|reflNP_999435.1 ; gi|6678509|reflNP_033500.1; gi|57463|emb|CAA31490.1 ; gi|20127395|reflNP_446220.1; gi| 137107|sp|P1 1645; gi|51458661 |ref|XP_497688.1 ; gi|207619|gb|AAA42318.1; gi|26340770|dbj|BAC34047.1 ; and gi|57459|emb|CAA30378.1. Each of these sequences and their annotations in the public databases accessible through the National Center for Biotechnology Information (NCBI) is incorporated by reference in its entirety. - 17 - [0054] In an embodiment of the invention, the uricase is truncated by 4 - 13 amino acids at its amino terminus. In an embodiment of the invention, the uricase is truncated by 4 - 13 amino acids at its carboxy temiinus. In an embodiment of the invention, the uricase is truncated by 4 -13 amino acids at both its carboxy and amino termini.
[0055] In an embodiment of the invention, the uricase is truncated by 6 amino acids at its amino terminus. In an embodiment of the invention, the uricase is truncated by 6 amino acids at its carboxy terminus. In an embodiment of the invention, the uricase is truncated by 6 amino acids at both its carboxy and amino termini.
[0056] In a particular embodiment, the uricase protein comprises the amino acid sequence from position 13 to position 292 of the amino acid sequence of pig uricase (SEQ ID NO. 11). In a particular embodiment, the uricase protein comprises the amino acid sequence from position 8 to position 287 of the amino acid sequence of PBC-ANC (SEQ ID NO. 12). In a particular embodiment, the uricase protein comprises the amino acid sequence from position 8 to position 287 of the amino acid sequence of Pig-KS-ΔΝ (SEQ ID NO. 7).
[0057] In another embodiment, the uricase protein comprises the amino acid sequence from position 44 to position 56 of Pig-KS-ΔΝ (SEQ ID NO. 14). This region of uricase has homology to sequences within the tunneling fold (T-fold) domain of uricase, and has within it a mutation at position 46 with respect to the native pig uricase sequence. This mutation surprisingly does not significantly alter the uricase activity of the protein.
[0058] In an embodiment of the invention, amino acids at or around any of amino acids 7, 46, and 291 , and 301 are mutated. In a preferred embodiment of the invention, amino acids 7, 46, and 291, and 301, themselves, are mutated. - 1 8 - [0059] In particular embodiments, the protein is encoded by a nucleic acid that encodes an N-terminal metliionine. Preferably, the N-terminal methionine is followed by a codon that allows for removal of this N-terminal methionine by bacterial methionine aminopeptidase (MAP). (Ben-Bassat and Bauer (1987) Nature 326:315, incorporated herein by reference in its entirety). Amino acids allowing the most complete removal of the N-terminal methionine are alanine, glycine, proline, serine, and threonine.
[0060] In an embodiment of the invention, the amino acids at or around positions 7 and/or 46 are substituted by threonine. Surprisingly, the enzymatic activity of truncated uricases prepared with these mutations is similar to that of the non-truncated enzyme. In a further embodiment of the invention, the amino acid mutations comprise threonine, threonine, lysine, and serine, at positions 7, 46, 291, and 301, respectively.
[0061] The truncated mammalian uricases disclosed herein may further comprise a metliionine at the amino terminus. The penultimate amino acid may one that allows removal of the N-terminal methionine by bacterial methionine aminopeptidase (MAP). Amino acids allowing the most complete removal of the N-terminal metliionine are alanine, glycine, proline, serine, and threonine. In a particular embodiment, the uricase comprises two amino terminal amino acids, wherein the two amino terminal amino acids are a methionine followed by an amino acid selected from the group consisting of alanine, glycine, proline, serine, and threonine.
[0062] In another embodiment of the invention, the substituted amino acids have been replaced by threonine.
[0063] In an embodiment of the invention, the uricase is a mammalian uricase. - 19 - [0064] In an embodiment of the invention, the mammalian uricase comprises the sequence of porcine, bovine, ovine or baboon liver uricase.
[0065] In an embodiment of the invention, the uricase is a chimeric uricase of two or more mammalian uricases.
[0066] In an embodiment of the invention, the mammalian uricases are selected from porcine, bovine, ovine, or baboon liver uricase.
[0067] In an embodiment of the invention, the uricase comprises the sequence of SEQ ID NO. 8.
[0068] In another embodiment of the invention, the uricase comprises the sequence of SEQ ID NO. 13.
[0069] The subject invention provides uricase encoding nucleic acids comprising the sequence of SEQ ID NO. 10.
[0070] In an embodiment of the invention, the uricase comprises fungal or microbial uricase.
[0071] In an embodiment of the invention, the fungal or microbial uricase is Aspergillus flavus, Arthrobacter globiformis or Candida utilis uricase.
[0072] In an embodiment of the invention, the uricase comprises an invertebrate uricase.
[0073] In an embodiment of the invention, the invertebrate uiicase Drosophila melanogaster or Drosophila pseudoobscura uiicase.
[0074] In an embodiment of the invention, the uricase comprises plant uricase. - 20 - [0075] In an embodiment of the invention, the plant uricase is Glycine max uricase of root nodules.
[0076] The subject invention provides a nucleic acid sequence encoding the uricase.
[0077] The subject invention provides a vector comprising the nucleic acid sequence.
[0078] In a particular embodiment, the uricase is isolated. In a particular embodiment, the uricase is purified. In particular embodiments, the uricase is isolated and purified.
[0079] The subject invention provides a host cell comprising a vector.
[0080] The subject invention provides a method for producing the nucleic acid sequence, comprising modification by PCR (polymerase chain reaction) techniques of a nucleic acid sequence encoding a nontruncated uricase. One skilled in the art knows that a desired nucleic acid sequence is prepared by PCR via synthetic oligonucleotide primers, which are complementary to regions of the target DNA (one for each strand) to be amplified. The primers are added to the target DNA (that need not- be pure), in the presence of excess deoxynucleotides and Taq polymerase, a heat stable DNA polymerase. In a series (typically 30) of temperature cycles, the target DNA is repeatedly denatured (around 90°C), annealed to the primers (typically at 50-60°C) and a daughter strand extended from the primers (72°C). As the daughter strands themselves act as templates for subsequent cycles, DNA fragments matching both primers are amplified exponentially, rather than linearly.
[0081] The subject invention provides a method for producing a mutant recombinant uricase comprising transfecting a host cell with the vector, wherein the host cell expresses the uricase, isolating the mutant recombinant uricase from the host cell, isolating the purified mutant - 21 - 186510/2 recombinant uricase using, for example, chromatographic techniques, and purifying the mutant recombinant uricase. For example, the uricase can be made according to the methods described in International Patent Publication No. WO 00/08196, incorporated herein by reference in its entirety.
[0082] The uricase may be isolated and or purified by any method known to those of skill in the art. Expressed polypeptides of this invention are generally isolated in substantially pure form. Preferably, the polypeptides are isolated to a purity of at least 80% by weight, more preferably to a purity of at least 95% by weight, and most preferably to a purity of at least 99% by weight. In general, such purification may be achieved using, for example, the standard techniques of ammonium sulfate fractionation, SDS-PAGE electrophoresis, and affinity chromatography. The uricase is preferably isolated using a cationic surfactant, for example, cetyl pyridinium chloride (CPC) according to the method described in copending United States patent application filed on April 11, 2005 having application no. 60/670,520 (unpublished U.S. provisional patent application) and attorney docket number 103864.146644, entitled Purification Of Proteins With Cationic Surfactant, incorporated herein by reference in its entirety.
[0083] In a preferred embodiment, the host cell is treated so as to cause the expression of the mutant recombinant uricase. One skilled in the art knows that transfection of cells with a vector is usually accomplished using DNA precipitated with calcium ions, though a variety of other methods can be used (e.g. electroporation).
[0084] In an embodiment of the invention, the vector is under the control of an osmotic pressure sensitive promoter. A promoter is a region of DNA to which RNA polymerase binds before initiating the transcription of DNA into RNA. An osmotic pressure sensitive promoter initiates transcription as a result of increased osmotic pressure as sensed by the cell. - 22 - [0085] In an embodiment of the invention, the promoter is a modified osmB promoter.
[0086] In particular embodiments, the uricase of the invention is a uricase conjugated with a polymer.
[0087] In an embodiment of the invention, a pharmaceutical composition comprising the uricase is provided. In one embodiment, the composition is a solution of uricase. In a preferred embodiment, the solution is sterile and suitable for injection. In one embodiment, such composition comprises uricase as a solution in phosphate buffered saline. In one embodiment, the composition is provided in a vial, optionally having a rubber injection stopper. In particular embodiments, the composition comprises uricase in solution at a concentration of from 2 to 16 milligrams of uricase per milliliter of solution, from 4 to 12 milligrams per milliliter or from 6 to 10 milligrams per milliliter. In a preferred embodiment, the composition comprises uricase at a concentration of 8 milligrams per milliliter. Preferably, the mass of uricase is measured with respect to the protein mass.
[0088] Effective administration regimens of the compositions of the invention may be determined by one of skill in the art. Suitable indicators for assessing effectiveness of a given regimen are known to those of skill in the art. Examples of such indicators include normalization or lowering of plasma uric acid levels (PUA) and lowering or maintenance of PUA to 6.8 mg/dL or less, preferably 6 mg/dL or less. Li a preferred embodiment, the subject being treated with the composition of the invention has a PUA of 6 mg/ml or less for at least 70%, at least 80%, or at least 90% of the total treatment period. For example, for a 24 week treatment period, the subject preferably has a PUA of 6 mg/ml or less for at least 80% of the 24 week treatment period, i.e., for at least a time equal to the amount of time in 134.4 days (24 weeks x 7 days/week x 0.8 = 134.4 days). - 23 - [0089] In particular embodiments, 0.5 to 24 mg of uricase in solution is administered once every 2 to 4 weeks. The uricase may be administered in any appropriate way known to one of skill in the art, for example, intravenously, intramuscularly or subcutaneously. Preferably, when the administration is intravenous, 0.5 mg to 12 mg of uricase is administered. Preferably, when the administration is subcutaneous, 4 to 24 mg of uricase is administered. In a preferred embodiment, the uricase is administered by intravenous infusion over a 30 to 240 minute period. In one embodiment, 8 mg of uricase is administered once every two weeks. In particular embodiments, the infusion can be performed using 100 to 500 mL of saline solution. In a preferred embodiment, 8 mg of uricase in solution is administered over a 120 minute period once every 2 weeks or once every 4 weeks; preferably the uricase is dissolved in 250 mL of saline solution for infusion. In particular embodiments, the uricase administrations take place over a treatment period of 3 months, 6 months, 8 months or 12 months. In other embodiments, the treatment period is 12 weeks, 24 weeks, 36 weeks or 48 weeks. In a particular embodiment, the treatment period is for an extended period of time, e.g., 2 years or longer, for up to the life of subject being treated. In addition, multiple treatment periods may be utilized interspersed with times of no treatment, e.g., 6 months of treatment followed by 3 months without treatment, followed by 6 additional months of treatment, etc.
[0090] In certain embodiments, anti-inflammatory compounds may be prophylactically administered to eliminate or reduce the occun-ence of infusion reactions due to the administration of uricase. In one embodiment, at least one corticosteroid, at least one antihistamine, at least one NSAID, or combinations thereof are so administered. Useful corticosteroids include betamethasone, budesonide, cortisone, dexamethasone, hydrocortisone, methylprednisolone, prednisolone, prednisone and triamcinolone. Useful NSAIDs include ibuprofen, indomethacin, naproxen, aspirin, acetaminophen, celecoxib and valdecoxib. Useful antihistamines include azatadine, brompheniramine, cetirizine, chlorpheniramine, clemastine, cyproheptadine, desloratadine, dexchlorpheniramine, dimenhydrinate, diphenhydramine, doxylamine, fexofenadine, hydroxyzine, loratadine and phenindamine.
[0091] In a preferred embodiment, the antihistamine is fexofenadine, the NSAID is acetaminophen and the corticosteroid is hydrocortisone and/or prednisone. Preferably, a combination of all three (not necessarily concomitantly) are administered prior to infusion of the uricase solution. In a preferred embodiment, the NSAID and antihistamine are administered orally 1 to 4 hours prior to uricase infusion. A suitable dose of fexofenadine includes about 30 to about 180 mg, about 40 to about 150 mg, about 50 to about 120 mg, about 60 to about 90 mg, about 60 mg, preferably 60 mg. A suitable dose of acetaminophen includes about 500 to about 1500 mg, about 700 to about 1200 mg, about 800 to about 1100 mg, about 1000 mg, preferably 1000 mg. A suitable dose of hydrocortisone includes about 100 to about 500 mg, about 150 to about 300 mg, about 200 mg, preferably 200 mg. In one embodiment, the antihistamine is not diphenhydramine. In another embodiment, the NSAID is not acetaminophen. In a preferred embodiment, 60 mg fexofenadine is administered orally the night before uricase infusion; 60 mg fexofenadine and 1000 mg of acetaminophen are administered orally the next morning, and finally, 200 mg hydrocortisone is administered just prior to the infusion of the uricase solution. In one embodiment, prednisone is administered the day, preferably in the evening, prior to uricase administration. An appropriate dosage of prednisone includes 5 to 50 mg, preferably 20 mg. In certain embodiments, these prophylactic treatments to eliminate or reduce the occurrence of infusion reactions are utilized for subjects receiving or about to receive uricase, including PEGylated uricase and non-PEGylated uricase. In particular embodiments, these prophylactic treatments are utilized for subjects receiving or about to receive therapeutic peptides other than uricase, wherein the other therapeutic peptides are PEGylated or non-PEGylated. - 25 - 186510/2
[0092] In an embodiment of the invention, the pharmaceutical composition comprises a uricase that has been modified by conjugation with a polymer, and the modified uricase retains uricolytic activity. In a particular embodiment, polymer-uricase conjugates are prepared as described in International Patent Publication No. WO 01/59078 and U.S. Application No. 09/501730 (Patent No. 6,783,965), incorporated herein by reference in their entireties.
[0093] In an embodiment of the invention, the polymer is selected from the group comprising polyethylene glycol, dextran, polypropylene glycol, hydroxypropylmethyl cellulose, carboxymethylcellulose, polyvinyl pyrrolidone, and polyvinyl alcohol.
[0094] In an embodiment of the invention, the composition comprises 2-12 polymer molecules on each uricase subunit, preferably 3 to 10 polymer molecules per uricase subunit.
[0095] In an embodiment of the invention, each polymer molecule has a molecular weight between about 1 kD and about 100 kD.
[0096] In another embodiment of the invention, each polymer molecule has a molecular weight between about 1 kD and about 50 kD. In a preferred embodiment of the invention, each polymer molecule has a molecular weight of between about 5 kD and about 20 kD, about 8 kD and about 15 kD, about 10 kD and 12 kD, preferably about 10 kD. In a preferred embodiment, each polymer molecule has a molecular weight of about 5 kD or about 20 kD. In an especially preferred embodiment of the invention, each polymer molecule has a molecular weight of 10 kD. Mixtures of different weight molecules are also contemplated. In an embodiment of the invention, the composition is suitable for repeated administration of the composition. - 26 - [0097] In a particular embodiment, conjugation of the uricase to the polymer comprises linkages selected from the group consisting of urethane linkages, secondary amine linkages, and amide linkages.
[0098] The subject invention provides a cell with the capacity for producing a uricase having an amino acid sequence of recombinant uricase, wherein the uricase has been truncated by 1 - 20 amino acids, and has mutated amino acids and uricolytic activity.
[0099] The subject invention provides a means for metabolizing uric acid using the uricase.
[0100] The subject invention provides a use of a composition of uricase for reducing uric acid levels in a biological fluid.
[0101] In an embodiment of the invention, the composition of uricase is used for reducing uric acid in a biological fluid comprising blood.
[0102] Also provided are novel nucleic acid molecules encoding uricase polypeptides. The manipulations which result in their production are well known to the one of skill in the art. For example, uricase nucleic acid sequences can be modified by any of numerous strategies known in the art (Maniatis, T., 1990, Molecular Cloning, A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.). The sequence can be cleaved at appropriate sites with restriction endonuclease(s), followed by further enzymatic modification if desired, isolated, and ligated in vitro. In the production of the gene encoding a uricase, care should be taken to ensure that the modified gene remains within the appropriate translational reading frame, uninterrupted by translational stop signals. Additionally, the uricase-encoding nucleic acid sequence can be mutated in vitro or in vivo, to create and or destroy translation, initiation, and/or termination sequences, or to create variations in coding regions and/or form new restriction - 27 -endonuclease sites or destroy preexisting ones, to facilitate further in vitro modification. Any technique for mutagenesis known in the art can be used, including but not limited to, in vitro site-directed mutagenesis (Hutchinson, C, et al., 1978, J. Biol. Chem 253 :6551), use of TAB® linkers (Pharmacia), etc.
[0103] The nucleotide sequence coding for a uricase protein can be inserted into an appropriate expression vector, i.e., a vector which contains the necessary elements for the transcription and translation of the inserted protein-coding sequence. A variety of host-vector systems may be utilized to express the protein-coding sequence. These include but are not limited to mammalian cell systems infected with virus (e.g., vaccinia virus, adenovirus, etc.); insect cell systems infected with virus (e.g., baculovirus); microorganisms such as yeast containing yeast vectors, or bacteria transformed with bacteriophage DNA, plasmid DNA, or cosmid DNA. The expression elements of these vectors vary in their strengths and specificities. Depending on the host-vector system utilized, any one of a number of suitable transcription and translation elements may be used.
[0104] Any of the methods known for the insertion of DNA fragments into a vector may be used to construct expression vectors containing a chimeric gene consisting of appropriate transcriptional/translational control signals and the protein coding sequences. These methods may include in vitro recombinant DNA and synthetic techniques and in vivo recombinations (genetic recombination). Expression of nucleic acid sequence encoding uricase protein may be regulated by a second nucleic acid sequence so that uricase protein is expressed in a host transformed with the recombinant DNA molecule. For example, expression of uricase may be controlled by any promoter/enhancer element known in the art. Promoters which may be used to control uricase expression include, but are not limited to, the SV40 early promoter region - 28 - (Bemoist and Chambon, 1981, Nature 290:304-310), the promoter contained in the 3' long terminal repeat of Rous sarcoma virus (Yamamoto, et al., 1980, Cell 22:787-797), the herpes thymidine kinase promoter (Wagner et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78: 144-1445), the regulatory sequences of the metallothioiiine gene (Brinster et al., 1982, Nature 296:39-42); prokaryotic expression vectors such as the β-lactamase promoter (Villa-Kamaroff, et al., 1978, Proc. Natl. Acad. Sci. U.S.A. 75:3727-3731), the tac promoter (DeBoer, et al., 1983, Proc. Natl. Acad. Sci. U.S.A. 80:21-25), and the osmB promoter. In particular embodiments, the nucleic acid comprises a nucleic acid sequence encoding the uricase operatively linked to a heterologous promoter.
[0105] Once a particular recombinant DNA molecule comprising a nucleic acid sequence encoding is prepared and isolated, several methods known in the art may be used to propagate it. Once a suitable host system and growth conditions are established, recombinant expression vectors can be propagated and prepared in quantity. As previously explained, the expression vectors which can be used include, but are not limited to, the following vectors or their derivatives: human or animal viruses such as vaccinia virus or adenovirus; insect viruses such as baculovirus; yeast vectors; bacteriophage vectors (e.g., lambda), and plasmid and cosmid DNA vectors, to name but a few.
[0106] In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Expression from certain promoters can be elevated in the presence of certain inducers; thus, expression of the genetically engineered uricase protein may be controlled. Furthermore, different host cells have characteristic and specific mechanisms for the translational and post-translational processing and modification (e.g., glycosylation, cleavage) of proteins. Appropriate - 29 - cell lines or host systems can be chosen to ensure the desired modification and processing of the foreign protein expressed. Different vector/host expression systems may effect processing reactions such as proteolytic cleavages to different extents.
[0107] In particular embodiments of the invention, expression of uricase in E. coli is preferably performed using vectors which comprise the osmB promoter.
EXAMPLES Example 1. Construction Of Gene And Expression Plasmid For Uricase Expression
[0108] Recombinant porcine uricase (urate oxidase), Pig-KS-ΔΝ (amino terminus truncated pig uricase protein replacing amino acids 291 and 301 with lysine and serine, respectively) was expressed in E. coli K-12 strain W3110 F-. A series of plasmids was constructed culminating in pOUR-P-AN-ks-l, which upon transformation of the E. coli host cells was capable of directing efficient expression of uricase.
Isolation And Subcloning Of Uricase cDNA From Pig And Baboon Liver
[0109] Uricase cDNAs were prepared from pig and baboon livers by isolation and subcloning of the relevant RNA. Total cellular RNA was extracted from pig and baboon livers (Erlich, H. A. (1989). PCR Technology; Principles and Application for DNA Amplification; Sambrook, J., et al. (1989). Molecular Cloning: A Laboratory Manual, 2nd edition; Ausubel, F.
M. et al. (1998). Current protocols in molecular Biology), then reverse-transcribed using the First-Strand cDNA Synthesis Kit (Pharmacia Biotech). PCR amplification was performed using Taq DNA polymerase (Gibco BRL, Life Technologies).
[0110] The synthetic oligonucleotide primers used for PCR amplification of pig and baboon urate oxidases (uricase) are shown in Table 1. - 30 - Table 1. Primers For PCR Amplification Of Uricase cDNA Pig liver uricase: sense 5 ' gcgcgaattcc ATGGCTC ATTACCGTAATGACTAC A 3 ' (SEQ ID NO. 1) anti-sense 5' gcgctctagaagcttccatggTCACAGCCTTGAAGTCAGC 3' (SEQ ID NO. 2) Baboon (D3H) liver uricase: sense 5' gcgcgaattccATGGCCCACTACCATAACAACTAT 3 ' (SEQ ID NO. 3) anti-sense 5 ' gcgcccatggtctagaTC AC AGTCTTGAAGAC AACTTCCT 3 ' (SEQ ID NO. 4)
[0111] Restriction enzyme sequences, introduced at the ends of the primers and shown in lowercase in Table 1, were sense EcoRI and Ncol (pig and baboon) and anti-sense Ncol, Hindlll and Xbal (pig), Xbal and Ncol (baboon). In the baboon sense primer, the third codon GAC (aspartic acid) present in baboon uricase was replaced with CAC (histidine), the codon that is present at this position in the coding sequence of the human urate oxidase pseudogene. The recombinant baboon uricase construct generated using these primers is named D3H Baboon Uricase.
[0112] The pig uricase PCR product was digested with EcoRI and Hindlll and cloned into pUCl 8 to create pUCl 8 - Pig Uricase. The D3H Baboon Uricase PCR product was cloned directly into pCR™II vector, using TA Cloning™ (Invitrogen, Carlsbad, CA), creating pCR™II -D3H Baboon Uricase. - 3 1 -
[0113] Ligated cDNAs were used to transform E. coli strain XLl-Blue (Stratagene, La Jolla, CA). Plasmid DNA containing cloned uricase cDNA was prepared, and clones which possess the published uricase DNA coding sequences (except for the D3H substitution in baboon uricase, shown in Table 1) were selected and isolated. In the pCR™II - D3H Baboon Uricase clone chosen, the pCR™II sequences were next to the uricase stop codon, resulting from deletion of sequences introduced by PCR. As a consequence, the Xbal and Ncol restriction sites from the 3' untranslated region were eliminated, thus allowing directional cloning using Ncol at the 5' end of the PCR product and BamHI which is derived from the pCR™II vector.
Subcloning Of Uricase cDNA Into pET Expression Vectors Baboon Uricase Subcloning
[0114] The D3H baboon cDNA containing full length uricase coding sequence was introduced into pET-3d expression vector (Novagen, Madison, WI). The pCR™II - D3H Baboon Uricase was digested with Ncol and BamHI, and the 960 bp fragment was isolated. The expression plasmid pET-3d was digested with Ncol and BamHI, and the 4600 bp fragment was isolated. The two fragments were ligated to create pET-3d-D3H-Baboon.
Pig-Baboon Chimera Uricase Subcloning
[0115] Pig-baboon chimera (PBC) uricase was constructed in order to gain higher expression, stability, and activity of the recombinant gene. PBC was constructed by isolating the 4936 bp Ncol-Apal fragment from pET-3d-D3H-Baboon clone and ligating the isolated fragment with the 624 bp Ncol-Apal fragment isolated from pUCl 8 -Pig Uricase, resulting in the formation of pET-3d-PBC. The PBC uricase cDNA consists of the pig uricase codons 1-225 joined in- frame to codons 226-304 of baboon uricase. - 32 - Pig-KS Uricase Subcloning
[0116] Pig-KS uricase was constructed in order to add one lysine residue, which may provide an additional PEGylation site. KS refers to the amino acid insert of lysine into pig uricase, at position 291 , in place of arginine (R291K). In addition, the threonine at position 301 was replaced with serine (T301 S). The PigKS uricase plasmid was constructed by isolating the 4696 bp Ncol-Ndel fragment of pET-3d-D3H-Baboon, and then it was ligated with the 864 bp Ncol-Ndel fragment isolated from pUC18 - Pig Uricase, resulting in the formation of pET-3d- PigKS. The resulting PigKS uricase sequence consists of the pig uricase codons 1-288 joined in- frame to codons 289-304 of baboon uricase.
Subcloning Of Uricase Sequence Under The Regulation Of The osmB Promoter
[0117] The uricase gene was subcloned into an expression vector containing the osmB promoter (following the teaching of Unites States Patent No. 5,795,776, incorporated herein by reference in its entirety). This vector enabled induction of protein expression in response to high osmotic pressure or culture aging. The expression plasmid pMFOA-18 contained the osmB promoter, a ribosomal binding site sequence (rbs) and a transcription terminator sequence (ter). It confers ampicillin resistance (AmpR) and expresses the recombinant human acetylcholine esterase (ACliE).
Subcloning Of D3H-Baboon Uricase
[0118] The plasmid pMFOA-18 was digested with Ncol and BamHI, and the large fragment was isolated. The construct pET-3d-D3H-Baboon was digested with Ncol and BamHI and the 960 bp fragment, which included the D3H Baboon Uricase gene is isolated. These two fragments were ligated to create pMFOU18.
[0119] The expression plasmid pMFXT133 contained the osmB promoter, a rbs (E. coli deo operon), ter (E. coli TrypA), the recombinant factor Xa inhibitor polypeptide (Fxal), and it conferred the tetracycline resistance gene (TetR). The baboon uricase gene was inserted into this - 33 - plasmid in order to exchange the antibiotic resistance genes. The plasmid pMFOU18 was digested with Ncol, filled-in, then it was digested with Xhol, and a 1030 bp fragment was isolated. The plasmid pMFXT133 was digested with Ndel, filled-in, then it was digested with Xhol, and the large fragment was isolated. The two fragments were ligated to create the baboon uricase expression vector, pURB A 16.
Subcloning Of The Pig Baboon Chimera Uricase
[0120] The plasmid pURBA 16 was digested with Apal and AlwNI, and the 2320 bp fragment was isolated. The plasmid MFXT133 was digested with Ndel, filled-in, then it was digested with AlwNI, and the 620 bp fragment was isolated. The construct pET-3d-PBC was digested with Xbal, filled-in, then it was digested with Apal, and the 710 bp fragment was isolated. The three fragments were ligated to create pUR-PB, a plasmid that expressed PBC uricase under the control of osmB promoter and rbs as well as the T7 rbs, which was derived from the pET-3d vector.
[0121] The T7 rbs was excised in an additional step. pUR-PB was digested with Ncol, filled- in, then digested with AlwNI, and the 3000 bp fragment was isolated. The plasmid pMFXTl 33 was digested with Ndel, filled in and then digested with AlwNI, and the 620 bp fragment was isolated. The two fragments were ligated to form pDUR-PB, which expresses PBC under the control of the osmB promoter.
Construction Of pOUR-PB-ANC
[0122] Several changes were introduced into the uricase cDNA, which resulted in a substantial increase in the recombinant enzyme stability. Plasmid pOUR-PBC-ANC was constructed, in which the N-terminal six-residue maturation peptide and the tri-peptide at the C- terminus, which function in vivo as peroxysomal targeting signal, were both removed. This was - 34 -carried out by utilizing PBC sequence in plasmid pDUR-PB and the specific oligonucleotide primers listed in Table 2, using PCR amplification.
Table 2. Primers for PCR Amplification of PBC-ANC Uricase PBC-ANC Uricase: Sense 5' gcgcatATGACTTACAAAAAGAATGATGAGGTAGAG 3' (SEQ ID NO. 5) Anti-sense 5' ccgtctagaTTAAGACAACTTCCTCTTGACTGTACCAGTAATTTTTCCGTATGG 3' (SEQ ID NO. 6)
[0123] The restriction enzyme sequences introduced at the ends of the primers shown in bold and the non-coding regions are shown in lowercase in Table 2. Ndel is sense and Xbal is anti-sense. The anti-sense primer was also used to eliminate an internal Ndel restriction site by-introducing a point mutation (underlined) which did not affect the amino acid sequence, and thus, facilitated subcloning by using Ndel.
[0124] The 900 base-pair fragment generated by PCR amplification of pDUR-PB was cleaved with Ndel and Xbal and isolated. The obtained fragment was then inserted into a deo expression plasmid pDBAST-RAT-N, which harbors the deo-PlP2 promoter and rbs derived from E. coli and constitutively expresses human recombinant insulin precursor. The plasmid was digested with Ndel and Xbal and the 4035 bp fragment was isolated and ligated to the PBC-uricase PCR product. The resulting construct, pDUR-PB-ANC, was used to transform E. coli K-12 S
[0125] The doubly truncated PBC-ANC sequence was also expressed under the control of osmB promoter. The plasmid pDUR-PB-ANC was digested with AlwNI - Ndel, and the 3459 bp - 35 - fragment was isolated. The plasmid pMFXT133, described above, was digested with Ndel - AlwNI, and the 660 bp fragment was isolated. The fragments were then ligated to create pOUR- PB-ANC, which was introduced into E. coli K-12 strain W31 10 F' and expressed high level of active truncated uricase.
Construction Of The Uricase Expression Plasmid pOUR-P-AN-ks-l
[0126] This plasmid was constructed in order to improve the activity and stability of the recombinant enzyme. Pig-KS-ΔΝ uricase was truncated at the N-terminus only (ΔΝ), where the six-residue N-terminal maturation peptide was removed, and contained the mutations S46T, R291K and T301 S. At position 46, there was a threonine residue instead of serine due to a conservative mutation that occurred during PCR amplification and cloning. At position 291, lysine replaced arginine, and at position 301 , serine was inserted instead of threonine. Both were derived from the baboon uricase sequence. The modifications of R291K and T301 S are designated KS, and discussed above. The extra lysine residue provided an additional potential PEGylation site.
[0127]- To construct pOUR-P-AN-ks-l (Figure 1), the plasmid pOUR-PB-ANC was digested with Apal - Xbal, and the 3873 bp fragment was isolated. The plasmid pET-3d-PKS (construction shown in Figure 4) was digested with Apal - Spel, and the 270 bp fragment was isolated. Spel cleavage left a 5' CTAG extension that was efficiently ligated to DNA fragments generated by Xbal. The two fragments were ligated to create pOUR-P-AN-ks-l. After ligation, the Spel and Xbal recognition sites were lost (their site is shown in parenthesis in Figure 9). The construct pOUR-P^N-ks-l was introduced into E. coli K-12 strain W31 10 F", prototrophic, ATCC # 27325. The resulting Pig-KS-ΔΝ uricase, expressed under the control of osmB promoter, yielded high levels of recombinant enzyme having superior activity and stability.
[0128] Figure 1 illustrates the structure of plasmid pOUR-P-AN-ks-l . Numbers next to restriction sites indicate nucleotide position, relative to Haell site, designated as 1 ; restriction sites that were lost during cloning are marked in parenthesis. Plasmid pOUR-P-AN-ks-l, encoding Pig-KS-ΔΝ uricase is 4143 base pairs (bp) long and comprised the following elements 1. A DNA fragment, 113 bp long, spanning from nucleotide number 1 to Ndel site (at position 1 13), which includes the osmB promoter and ribosome binding site (rbs). 2. A DNA fragment, 932 bp long, spanning from Ndel (at position 113) to Spel/Xbal junction (at position 1045), which includes: 900 bp of Pig-KS-ΔΝ (nucleic acid sequence of amino terminus truncated pig uricase protein in which amino acids 291 and 301 with lysine and serine, respectively, are replaced) coding region and 32 bp flanking sequence derived from pCR™ II, from the TA cloning site upstream to the Spel/Xbal restriction site. 3. A 25 bp multiple cloning sites sequence (MCS) from Spel/Xbal junction (at position 1045) to Hindlll (at position 1070). 4. A synthetic 40 bp oligonucleotide containing the TrpA transcription terminator (ter) with Hindlll (at position 1070) and Aatll (at position 1 110) ends. 5. A DNA fragment, 1519 bp long, spanning from Aatll (at position 11 10) to Mscl/Scal (at position 2629) sites on pBR322 that includes the tetracycline resistance gene (TetR). 6. A DNA fragment, 1514 bp long, spanning from Seal (at position 2629) to Haell (at position 4143) sites on pBR322 that includes the origin of DNA replication.
[0129] Figure 2 shows the DNA and the deduced amino acid sequences of Pig-KS-ΔΝ uricase. In this figure, the amino acid numbering is according to the complete pig uricase - 37 -sequence. Following the initiator methionine residue, a threonine was inserted in place of the aspartic acid of the pig uricase sequence. This threonine residue enabled the removal of methionine by bacterial aminopeptidase. The gap in the amino acid sequence illustrates the deleted N-terminal maturation peptide. The restriction sites that were used for the various steps of subcloning of the different uricase sequences (Apal, Ndel, BamHI, EcoRI and Spel) are indicated. The 3' untranslated sequence, shown in lowercase letters, was derived from pCR™II sequence. The translation stop codon is indicated by an asterisk.
[0130] Figure 3 shows alignment of the amino acid sequences of the various recombinant uricase sequences. The upper line represents the pig uricase, which included the full amino acid sequence. The second line is the sequence of the doubly truncated pig'-baboon chimera uricase (PBC-ANC). The third line shows the sequence of Pig-KS-ΔΝ uricase, that is only truncated at the N-terminus and contained the mutations S46T and the amino acid changes R291K and T301 S, both reflecting the baboon origin of the carboxy terminus of the uricase coding sequence. The asterisks indicate the positions in which there are differences in amino acids in the Pig-KS-ΔΝ as compared to the published pig uricase sequence; the circles indicate positions in which there are differences in amino acids in Pig-KS-ΔΝ compared to PBC-ΔΝ, the pig-baboon chimera; and dashed lines indicate deletion of amino acids.
[0131] cDNA for native baboon, pig, and rabbit uricase with the Y97H mutation, and the pig/baboon chimera (PBC) were constructed for cloning into E. coli. Clones expressing high levels of the uricase variants were constructed and selected such that all are W31 10 F" E. coli, and expression is regulated by osmB. Plasmid DNAs were isolated, verified by DNA sequencing and restriction enzyme analysis, and cells were cultured.
[0132] Construction of the truncated uricases, including pig-ΔΝ and Pig-KS-ΔΝ was done by cross-ligation between PBC-ANC and Pig-KS, following cleavage with restriction - 38 - endonucleases Apal and Xbal, and Apal plus Spel, respectively. It is reasonable that these truncated mutants would retain activity, since the N-terminal six residues, the "maturation peptide" (1-2), and the C-tenninal tri-peptide, "peroxisomal targeting signal" (3-5), do not have functions which significantly affect enzymatic activity, and it is possible that these sequences may be immunogenic. Clones expressing very high levels of the uricase variants were selected.
Example 2. Transformation Of The Expression Plasmid Into A Bacterial Host Cell
[0133] The expression plasmid, pOUR-P-AN-ks-l, was introduced into E. coli K-12 strain W31 10 F". Bacterial cells were prepared for transformation involved growth to mid log phase in Luria broth (LB), then cells were harvested by centrifugation, washed in cold water, and suspended in 10% glycerol, in water, at a concentration of about 3xl010 cells per ml. The cells were stored in aliquots, at -70°C. Plasmid DNA was precipitated in ethanol and dissolved in water.
[0134] Bacterial cells and plasmid DNA were mixed, and transformation was done by the high voltage electroporation method using Gene Pulser II from BIO-RAD (Trevors et al (1992).
Electrotransformation of bacteria by plasmid DNA, in Guide to Electroporation and Electrofusion (D.C. Chang, B. M. Chassy, J. A. Saunders and A. E. Sowers, eds.), pp. 265-290, Academic Press Inc., San Diego, Hanahan et al (1991) Meth. Enzymol., 204, 63-113).
Transformed cells were suspended in SOC medium (2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5mM KC1, 10 mM MgCl2, 10 mM MgS04, 20 mM glucose), incubated, at 37°C, for 1 hour and selected for tetracycline resistance. A high expresser clone was selected.
Example 3. Recombinant Uricase Preparation
[0135] Bacteria such as those transformed (see above) were cultured in medium containing glucose; pH was maintained at 7.2 ± 0.2, at approximately 37°C. - 39 - [0136] Towards the last 5-6 hours of cultivation, the medium was supplemented with KC1 to a final concentration of 0.3M. Cultivation was continued to allow uricase accumulation.
[0137] Recombinant uricase accumulated within bacterial cells as an insoluble precipitate similar to inclusion bodies (IBs). The cell suspension was washed by centrifugation and suspended in 50 mM Tris buffer, pH 8.0 and 10 mM EDTA and brought to a final volume of approximately 40 times the dry cell weight.
[0138] Recombinant uricase-containing IBs, were isolated by centrifugation following disruption of bacterial cells using lysozyme and high pressure. Treatment with lysozyme (2000-3000 units/ml) was done for 16-20 hours at pH 8.0 and 7 ± 3°C, while mixing. The pellet was washed with water and stored at -20°C until use.
[0139] The enriched IBs were further processed after suspending in 50 mM NaHC03 buffer, pH 10.3±0.1. The suspension was incubated overnight, at room temperature, to allow solubilization of the IB-derived uricase, and subsequently clarified by centrifugation.
[0140] Uricase was further purified by several chromatography steps. Initially, chromatography was done on a Q-Sepharose FF column. The loaded column was washed with bicarbonate buffer containing 150 mM NaCl, and uricase was eluted with bicarbonate buffer, containing 250 mM NaCl. Then, Xanthine-agarose resin (Sigma) was used to remove minor impurities from the uricase preparation. The Q-Sepharose FF eluate was diluted with 50 mM glycine buffer, pH 10.3±0.1, to a protein concentration of approximately 0.25 mg/ml and loaded. The column was washed with bicarbonate buffer, pH 10.3±0.1, containing 100 mM NaCl, and uricase was eluted with the same buffer supplemented with 60 μΜ xanthine. At this stage, the uricase was repurified by Q-Sepharose chromatography to remove aggregated forms. - 40 -
[0141] The puiity of each uricase preparation is greater than 95%, as determined by size exclusion chromatography. Less than 0.5% aggregated forms are detected in each preparation using a Superdex 200 column.
[0142] Table 3 summarizes purification of Pig-KSAN uricase from IBs derived from 25 L fermentation broth.
Table 3. Purification Of Pig-KSAN Uricase Example 4. Characteristics Of Recombinant Uricases SDS-PAGE
[0143] SDS-PAGE analysis of the highly purified uricase variants (Figure 4) revealed a rather distinctive pattern. The samples were stored at 4°C, in carbonate buffer, pH 10.3, for up to several months. The full-length variants, Pig, Pig-KS, and PBC, show accumulation of two major degradation products having molecular weights of about 20 and 15kD. This observation suggests that at least a single nick split the uricase subunit molecule. A different degradation pattern is detected in the amino terminal shortened clones and also in the rabbit uricase, but at a lower proportion. The amino terminus of the rabbit resembles that of the shortened clones. The amino terminal sequences of the uricase fragments generated during purification and storage were determined. - 41 - Peptide Sequencing
[0144] N-terminal sequencing of bulk uricase preparations was done using the Edman degradation method. Ten cycles were performed. Recombinant Pig uricase (full length clone) generated a greater abundance of degradation fragments compared to Pig-KS-ΔΝ. The deduced sites of cleavage leading to the degradation fragments are as follows: 1) Major site at position 168 having the sequence: -QSG I FEGFI- 2) Minor site at position 142 having the sequence: --IRN 4 GPPVI-
[0145] The above sequences do not suggest any known proteolytic cleavage. Nevertheless, cleavage could arise from either proteolysis or some chemical reaction. The amino-truncated uricases are surprisingly more stable than the non-amino truncated uricases. PBC-ANC also had stability similar to the other ΔΝ molecules and less than non-amino-truncated PBC.
Potency
[0146] Activity of uricase was measured by a UV method. Enzymatic reaction rate was determined by measuring the decrease in absorbance at 292 nm resulting from the oxidation of uric acid to allantoin. One activity unit is defined as the quantity of uricase required to oxidize one μπιοΐε of uric acid per minute, at 25°C, at the specified conditions. Uricase potency is expressed in activity units per mg protein (U/mg).
[0147] The extinction coefficient of 1 mM uric acid at 292 nm is 12.2 mM'1 cm"1. Therefore, oxidation of 1 μηιοΐε of uric acid per ml reaction mixture resulted in a decrease in absorbance of 12.2 mA292. The absorbance change with time (ΔΑ292 per minute) was derived from the linear portion of the curve. - 42 - [0148] Protein concentration was determined using a modified Bradford method (Macart and Gerbaut (1982) Clin Chim Acta 122:93-101). The specific activity (potency) of uricase was calculated by dividing the activity in U/ml with protein concentration in mg/ml. The enzymatic activity results of the various recombinant uricases are summarized in Table 4. The results of commercial preparations are included in this table as reference values. It is apparent from these results that truncation of ui'icase proteins has no significant effect on their enzymatic activity.
Table 4. Summary of Kinetic Parameters of Recombinant and Native Uricases
[0149] Table 4 Notes: (1) Protein concentration was determined by absorbance measured at 278 nm, using an Extinction coefficient of 11.3 for a 10 mg/ml uricase solution (Mahler, 1963). (2) 1 unit of uricase activity is defined as the amount of enzyme that oxidizes 1 μιηοΐε of uric acid to allantoin per minute, at 25°C. (3) Specific activity values were derived from the Lineweaver-Burk plots, at a concentration of substrate equivalent to 60 μΜ. - 43 - (4) Reaction Mixtures were composed of various combinations of the following stock solutions 100 mM sodium borate buffer, pH 9.2 300 μΜ Uric acid in 50 mM sodium borate buffer, pH 9.2 1 mg/ml BSA in 50 mM sodium borate buffer, pH 9.2 (5) Kcat was calculated by dividing the Vmax (calculated from the respective Lineweaver- Burk plots) by the concentration of uricase in reaction mixture (expressed in mol equivalents, based on the tetrameric molecular weights of the uricases).
Example 5. Conjugation Of Uricase With m-PEG (PEGylation)
[0150] Pig-KS-ΔΝ Uricase was conjugated using m-PEG-NPC (monomethoxy- poly(ethylene glycol)-nitrophenyl carbonate). Conditions resulting in 2 - 12 strands of 5, 10, or 20 kD PEG per uricase subunit were established. m-PEG-NPC was gradually added to the protein solution. After PEG addition was concluded, the uricase / m-PEG-NPC reaction mixture was then incubated at 2-8°C for 16-18 hours, until maximal unbound m-PEG strands were conjugated- to uricase.
[0151] The number of PEG strands per PEG-uricase monomer was determined by Superose 6 size exclusion chromatography (SEC), using PEG and uricase standards. The number of bound PEG strands per subunit was determined by the following equation: PEG 3.42 x Amount of PEG in injected sample ^g) strands/subunit = Amount of protein in injected sample (μσ)
[0152] The concentration of PEG and protein moieties in the PEG-uricase sample was determined by size exclusio BACKGROUND OF THE INVENTIONn cliiOmatography (SEC) using ultraviolet (UV) and refractive index (RI) detectors arranged in series (as developed by Kunitani, et al., 1991). Three calibration curves are generated: a protein curve (absorption - 44 - measured at 220 nm); a protein curve (measured by RI); and PEG curve (measured by RI). Then, the PEG-uricase samples were analyzed using the same system. The resulting UV and RI peak area values of the experimental samples were used to calculate the concentrations of the PEG and protein relative to the calibration curves. The index of 3.42 is the ratio between the molecular weight of uricase monomer (34,192 Daltons) to that of the 10 kD PEG.
[0153] Attached PEG improved the solubility of uricase in solutions having physiological pH values. Table 5 provides an indication of the variability between batches of PEGylated Pig- S-ΔΝ uricase product. In general, there is an inverse relation between the number of PEG strands attached and retained specific activity (SA) of the enzyme.
Table 5. Enzymatic Activity Of PEGylated Pig-KS-ΔΝ Uricase Conjugates Example 6. PEGylation of uricase with 1000 D and 100,000 D PEG
[0154] Pig- S-ΔΝ Uricase was conjugated using 1000 D and 100,000 D m-PEG-NPC as described in Example 5. Conditions resulting in 2 - 11 strands of PEG per uricase subunit were used. After PEG addition was concluded, the uricase/m-PEG-NPC reaction mixture was then - 45 - incubated at 2-8°C for 16-18 hours, until maximal unbound m-PEG strands were conjugated to uricase.
[0155] The number of PEG strands per PEG-uricase monomer was detemiined as described above.
[0156] Attached PEG improved the solubility of uricase in solutions having physiological pH values.
Example 7. Pharmacokinetics Of Pig-KS-ΔΝ Uricase Conjugated With PEG
[0157] Biological experiments were undertaken in order to determine the optimal extent and size of PEGylation needed to provide therapeutic benefit.
[0158] Pharmacokinetic studies in rats, using i.v. injections of 0.4 mg (2U) per kg body weight of unmodified uricase, administered at day 1 and day 8, yielded a circulating half life of about 10 minutes. However, studies of the clearance rate in rats with 2-1 lxlOkD PEG-Pig-KS- ΔΝ uricase, after as many as 9 weekly injections, indicated that clearance did not depend on the number of PEG strands (within this range) and remained relatively constant throughout the study period (see Table 6; with a half-life of about 30 hours). The week-to-week differences are within experimental error. This same pattern is apparent after nine injections of the 10x5kD PEG, and 10x20kD PEG - uricase conjugates. The results indicated that regardless of the extent of uricase PEGylation, in this range, similar biological effects were observed in the rat model. - 46 - Table 6. Half Lives of PEGylated Pig-KS-ΔΝ Uricase Preparations in Rats
[0159] Table 6 notes: Results are indicated in hours ± standard error of the mean. Numbers in parenthesis indicate the number of animals tested.
[0160] Rats received weekly i.v. injections of 0.4 mg per kilogram body weight of Pig-KS-ΔΝ uricase modified as indicated in the table. Each group initially comprised 15 rats, which were alternately bled in subgroups of 5. Several rats died during the study due to the anesthesia. Half-lives were determined by measuring uiicase activity (colorimetric assay) in plasma samples collected at 5 minutes, and 6, 24 and 48 hours post injection.
[0161] Table 5 describes the batches of PEGylated uricase used in the study. - 47 - [0162] Bioavailability studies with 6x5 kD PEG- Pig-KS-ΔΝ uricase in rabbits indicate that, after the first injection, the circulation half-life is 98.2 ± 1.8 hours (i.v.), and the bioa\¾ilability after i.m. and subcutaneous (s.c.) injections was 71 % and 52%, respectively. However, significant anti-uricase antibody titers were detected, after the second i.m. and s.c. injections, in all of the rabbits, and clearance was accelerated following subsequent injections. Injections of rats with the same conjugate resulted in a half-life of 26±1.6 hours (i.v.), and the bioavailability after i.m. and s.c. injections was 33% and 22%, respectively.
[0163] Studies in rats, with 9x10 kf) PEG- Pig-KS-ΔΝ uricase indicate that the circulation half-life after the first injection is 42.4 hours (i.v.), and the bioavailability, after i.m. and s.c. injections, was 28.9% and 14.5%, respectively (see Figure 5 and Table 7). After the fourth injection, the circulation half-life was 32.1 ± 2.4 hours and the bioavailability, after the i.m. and s.c. injections was 26.1% and 14.9%, respectively.
[0164] Similar pharmacokinetic studies, in rabbits, with 9x10 kD PEG-Pig-KS-ΔΝ uricase indicate that no accelerated clearance was observed following injection of this conjugate (4 biweekly injections were administered). In these animals, the circulation half-life after the first injection was 88.5 hours (i.v.), and the bioavailability, after i.m. and s.c. injections, was 98.3% and 84.4%), respectively (see Figure 6 and Table 7). After the fourth injection the circulation half-life was 141.1±15.4 hours and the bioavailability, after the i.m. and s.c. injections was 85% and 83%, respectively.
[0165] Similar studies with 9x10 kD PEG-Pig-KS-ΔΝ were done to assess the bioavailability in beagles (2 males and 2 females in each group). A circulation half-life of 70±1 1.7 hours was recorded after the first i.v. injection, and the bioavailability, after the i.m. and s.c. injections was 69.5%) and 50.4%, respectively (see Figure 7 and Table 7). - 48 - [0166] Studies with 9x10 kD PEG-Pig-KS-ΔΝ preparations were done using pigs. Three animals per group were used for administration via the i.v., s.c. and i.m. routes. A circulation half-life of 178±24 hours was recorded after the first i.v. injection, and the bioavailability, after the i.m. and s.c. injections was 71.6% and 76.8%, respectively (see Figure 8 and Table 7).
Table 7. Pharmacokinetic Studies with 9x10 kD PEG-Pig-KS-ΔΝ Uricase
[0167] Absorption, distribution, metabolism, and excretion (ADME) studies were done after iodination of 9x1 OkD PEG-Pig-KS-ΔΝ uricase by the Bolton & Hunter method with 125I. The labeled conjugate was injected into 7 groups of 4 rats each (2 males and 2 females). Distribution of radioactivity was analyzed after 1 hour and every 24 hours for 7 days (except day 5). Each group, in its turn, was sacrificed and the different organs were excised and analyzed. The seventh group was kept in a metabolic cage, from which the urine and feces were collected. The distribution of the material throughout the animal's body was evaluated by measuring the total radioactivity in each organ, and the fraction of counts (kidney, liver, lung, and spleen) that were available for precipitation with TCA (i.e. protein bound, normalized to the organ size). Of the organs that were excised, none had a higher specific radioactivity than the others, thus no - 49 - significant accumulation was seen for instance in the liver or kidney. 70% of the radioactivity was excreted by day 7.
Example 8. Clinical Trial Results
[0168] A randomized, open-label, multicenter, parallel group study was performed to assess the urate response, and pharmacokinetic and safety profiles of PEG-uricase (Puricase®, Savient Pharmaceuticals) in human patients with rryperuricemia and severe gout who were unresponsive to or intolerant of conventional therapy. The mean duration of disease was 14 years and 70 percent of the study population had one or more tophi.
[0169] In the study, 41 patients (mean age of 58.1 years) were randomized to 12 weeks of treatment with intravenous PEG-uricase at one of four dose regimens: 4 mg every two weeks (7 patients); 8 mg every two weeks (8 patients); 8 mg every four weeks (13 patients); or 12 mg every four weeks (13 patients). Plasma uricase activity and urate levels were measui'ed at defined intervals. Pharmacokinetic parameters, mean plasma urate concentration and the percentage of time that plasma urate was less than or equal to 6 mg/dL were derived from analyses of the uricase activities and urate levels.
[0170] Patients who received 8 mg of PEG-uricase every two weeks had the greatest reduction in PUA with levels below 6 mg/dL 92 percent of the treatment time (pre-treatment plasma urate of 9.1 mg/dL vs. mean plasma urate of 1.4 mg/dL over 12 weeks).
[0171] Substantial and sustained lower plasma urate levels were also observed in the other PEG-uricase treatment dosing groups: PUA below 6 mg/ml 86 percent of the treatment time in the 8 mg every four weeks group (pre-treatment plasma urate of 9.1 mg/dL vs. mean plasma urate of 2.6mg/dL over 12 weeks); PUA below 6 mg/ml 84 percent of the treatment time in the 12 mg every four weeks group (pre-treatment plasma urate of 8.5 mg/dL vs. mean plasma urate of 2.6 mg/dL over 12 weeks); and PUA below 6 mg/ml 73 percent of the treatment time in the 4 - 50 -mg every two weeks group (pre-treatment plasma urate of 7.6 mg/dL vs. mean plasma urate of 4.2 mg/dL over 12 weeks).
[0172] The maximum percent decrease in plasma uric acid from baseline within the first 24 hours of PEG-uricase dosing was 72% for subjects receiving 4 mg / 2 weeks (p equals .0002); 94% for subjects receiving 8 mg / 2 weeks (p less than .0001); 87% for subjects receiving 8 mg / 4 weeks (p less than .0001); and 93% for subjects receiving 12 mg / 4 weeks (p less than .0001).
[0173] The percent decrease in plasma uric acid from baseline over the 12-week treatment period was 38% for subjects receiving 4 mg / 2 weeks (p equals .0002); 86% for subjects receiving 8 mg / 2 weeks (p less than .0001); 58% for subjects receiving 8 mg / 4 weeks (p equals .0003); and 67% for subjects receiving 12 mg / 4 weeks (p less than .0001).
[0174] Surprisingly, some subjects receiving PEG-uricase experienced an infusion related adverse event, i.e., an infusion reaction. These reactions occurred in 14% of the total infusions.
[0175] All references cited herein are incorporated herein by reference in their entirety and for all purposes to the same extent as if each individual publication or patent or patent application was specifically and individually indicated to be incorporated by reference in its entirety for all purposes.
[0176] Many modifications and variations of the present invention can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. The specific embodiments described herein are offered by way of example only, and the invention is to be limited only by the terms of the appended claims along with the full scope of equivalents to which such claims are entitled. - 51 - SEQUENCE LISTING <110> Savient Pharmaceuticals, Inc.
Hartman, Jacob Mendelovitz, Simona <120> VARIANT FORMS OF URATE OXIDASE AND USE THEREOF <130> 103864-146638 <150> US 60/670,573 <151> 2005-04-11 <160> 16 <170> Patentln version 3.3 <210> 1 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> Pig Liver Uricase (sense) <400> 1 gcgcgaattc catggctcat taccgtaatg actaca <210> 2 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> Pig Liver Uricase (antisense) <400> 2 gcgctctaga agcttccatg gtcacagcct tgaagtcagc <210> 3 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> Baboon (D3H) Liver Uricase (sense) <400> 3 gcgcgaattc catggcccac taccataaca actat <210> 4 <211> 40 <212> DNA <213> Artificial Sequence <220> <223> Baboon (D3H) Liver Uricase (antisense) 01783984\90-01 - 52 - <400> 4 gcgcccatgg tctagatcac agtcttgaag acaacttcct <210> 5 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> PBC-DeltaNC Uricase <400> 5 gcgcatatga cttacaaaaa gaatgatgag gtagag <210> 6 <211> 54 <212> DNA <213> Artificial Sequence <220> <223> PBC-DeltaNC Uricase <400> 6 ccgtctagat taagacaact tcctcttgac tgtaccagta atttttccgt atgg <210> 7 <211> 299 <212> PRT <213> Artificial Sequence <220> <223> Pig-KS-DeltaN <400> 7 Met Thr Tyr Lys Lys Asn Asp Glu Val Glu Phe Val Arg Thr Gly Tyr 1 5 10 15 Gly Lys Asp Met He Lys Val Leu His He Gin Arg Asp Gly Lys Tyr 20 25 30 His Ser He Lys Glu Val Ala Thr Thr Val Gin Leu Thr Leu Ser Ser 35 40 45 Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp Val He Pro Thr Asp 50 55 60 Thr He Lys Asn Thr Val Asn Val Leu Ala Lys Phe Lys Gly He Lys 65 70 75 80 Ser He Glu Thr Phe Ala Val Thr He Cys Glu His Phe Leu Ser Ser 85 90 95 Phe Lys His Val He Arg Ala Gin Val Tyr Val Glu Glu Val Pro Trp 01783984\90-01 - 53 - 100 105 110 Lys Arg Phe Glu Lys Asn Gly Val Lys His Val His Ala Phe He Tyr 115 120 125 Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu Gin He Arg Asn Gly 130 135 140 Pro Pro Val He His Ser Gly He Lys Asp Leu Lys Val Leu Lys Thr 145 150 155 160 Thr Gin Ser Gly Phe Glu Gly Phe He Lys Asp Gin Phe Thr Thr Leu 165 170 175 Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gin Val Tyr Cys Lys Trp 180 185 190 Arg Tyr His Gin Gly Arg Asp Val Asp Phe Glu Ala Thr Trp Asp Thr 195 200 205 Val Arg Ser He Val Leu Gin Lys Phe Ala Gly Pro Tyr Asp Lys Gly 210 215 220 Glu Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr Asp He Gin Val Leu 225 230 235 240 Thr Leu Gly Gin Val Pro Glu He Glu Asp Met Glu He Ser Leu Pro 245 250 255 Asn He His Tyr Leu Asn He Asp Met Ser Lys Met Gly Leu He Asn 260 265 270 Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro Tyr Gly Lys He Thr 275 280 285 Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 290 295 <210> 8 <211> 298 <212> PRT <213> Artificial Sequence <220> <223> Pig- S-DeltaN without <400> 8 Thr Tyr Lys Lys Asn Asp Glu Val Glu Phe Val Arg Thr Gly Tyr Gly 1 5 10 15 01783984\90-01 - 54 - Lys Asp Met lie Lys Val Leu His lie Gin Arg Asp Gly Lys Tyr His 20 25 30 Ser lie Lys Glu Val Ala Thr Thr Val Gin Leu Thr Leu Ser Ser Lys 35 40 45 Lys Asp Tyr Leu His Gly Asp Asn Ser Asp Val lie Pro Thr Asp Thr 50 55 60 lie Lys Asn Thr Val Asn Val Leu Ala Lys Phe Lys Gly lie Lys Ser 65 70 75 80 lie Glu Thr Phe Ala Val Thr lie Cys Glu His Phe Leu Ser Ser Phe 85 90 95 Lys His Val He Arg Ala Gin Val Tyr Val Glu Glu Val Pro Trp Lys 100 105 110 Arg Phe Glu Lys Asn Gly Val Lys His Val His Ala Phe He Tyr Thr 115 120 125 Pro Thr Gly Thr His Phe Cys Glu Val Glu Gin He Arg Asn Gly Pro 130 135 140 Pro Val He His Ser Gly He Lys Asp Leu Lys Val Leu Lys Thr Thr 145 150 155 160 Gin Ser Gly Phe Glu Gly Phe He Lys Asp Gin Phe Thr Thr Leu Pro 165 170 175 Glu Val Lys Asp Arg Cys Phe Ala Thr Gin Val Tyr Cys Lys Trp Arg 180 185 190 Tyr His Gin Gly Arg Asp Val Asp Phe Glu Ala Thr Trp Asp Thr Val 195 200 205 Arg Ser He Val Leu Gin Lys Phe Ala Gly Pro Tyr Asp Lys Gly Glu 210 215 220 Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr Asp He Gin Val Leu Thr 225 230 235 240 Leu Gly Gin Val Pro Glu He Glu Asp Met Glu He Ser Leu Pro Asn 245 250 255 He His Tyr Leu Asn He Asp Met Ser Lys Met Gly Leu He Asn Lys 01783984\90-01 IL 186510/3 55- 260 265 270 Glu Glu Val Leu Leu Pro Leu Asp Asn Pro Tyr Gly Lys lie Thr Gly 275 280 285 Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 290 295 <210> 9 <211> 897 <212> DNA <213> Artificial Sequence <220> <223> Pig-KS-DeltaNA <400> 9 atgacttaca aaaagaatga tgaggtagag tttgtccgaa ctggctatgg gaaggatatg 60 ataaaagttc tccatattca gcgagatgga aaatatcaca gcattaaaga ggtggcaact 120 acagtgcaac tgactttgag ctccaaaaaa gattacctgc atggagacaa ttcagatgtc 180 atccctacag acaccatcaa gaacacagtt aatgtcctgg cgaagttcaa aggcatcaaa 240 agcatagaaa cttttgctgt gactatctgt gagcatttcc tttcttcctt caagcatgtc 300 atcagagctc aagtctatgt ggaagaagtt ccttggaagc gttttgaaaa gaatggagtt 360 aagcatgtcc atgcatttat ttatactcct actggaacgc acttctgtga ggttgaacag 420 ataaggaatg gacctccagt cattcattct ggaatcaaag acctaaaagt cttgaaaaca 480 acccagtctg gctttgaagg attcatcaag gaccagttca ccaccctccc tgaggtgaag 540 gaccggtgct ttgccaccca agtgtactgc aaatggcgct accaccaggg cagagatgtg 600 gactttgagg ccacctggga cactgttagg agcattgtcc tgcagaaatt tgctgggccc 660 tatgacaaag gcgagtactc gccctctgtc cagaagacac tctatgacat ccaggtgctc 720 accctgggcc aggttcctga gatagaagat atggaaatca gcctgccaaa tattcactac 780 ttaaacatag acatgtccaa aatgggactg atcaacaagg aagaggtctt gctaccttta 840 gacaatccat atggaaaaat tactggtaca gtcaagagga agttgtcttc aagactg 897 <210> 10 <211> 894 <212> DNA <213> Artificial Sequence <220> <223> Pig- S-DeltaN without starting ATG <400> 10 acttacaaaa agaatgatga ggtagagttt gtccgaactg gctatgggaa ggatatgata 60 aaagttctcc atattcagcg agatggaaaa tatcacagca ttaaagaggt ggcaactaca 120 01783984\90-01 IL 186510/3 -56- gtgcaactga ctttgagctc caaaaaagat tacctgcatg gagacaattc agatgtcatc 180 cctacagaca ccatcaagaa cacagttaat gtcctggcga agttcaaagg catcaaaagc 240 atagaaactt ttgctgtgac tatctgtgag catttccttt cttccttcaa gcatgtcatc 300 agagctcaag tctatgtgga agaagttcct tggaagcgtt ttgaaaagaa tggagttaag 360 catgtccatg catttattta tactcctact ggaacgcact tctgtgaggt tgaacagata 420 aggaatggac ctccagtcat tcattctgga atcaaagacc taaaagtctt gaaaacaacc 480 cagtctggct ttgaaggatt catcaaggac cagttcacca ccctccctga ggtgaaggac 540 cggtgctttg ccacccaagt gtactgcaaa tggcgctacc accagggcag agatgtggac 600 tttgaggcca cctgggacac tgttaggagc attgtcctgc agaaatttgc tgggccctat 660 gacaaaggcg agtactcgcc ctctgtccag aagacactct atgacatcca ggtgctcacc 720 ctgggccagg ttcctgagat agaagatatg gaaatcagcc tgccaaatat tcactactta 780 aacatagaca tgtccaaaat gggactgatc aacaaggaag aggtcttgct acctttagac 840 aatccatatg gaaaaattac tggtacagtc aagaggaagt tgtcttcaag actg 894 <210> 11 <211> 304 <212> PRT <213> Sus scrofa <400> 11 Met Ala His Tyr Arg Asn Asp Tyr Lys Lys Asn Asp Glu Val Glu Phe Val Arg Thr Gly Tyr Gly Lys Asp Met lie Lys Val Leu His lie Gin 20 25 30 Arg Asp Gly Lys Tyr His Ser lie Lys Glu Val Ala Thr Ser Val Gin 35 40 45 Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 50 55 60 Val lie Pro Thr Asp Thr lie Lys Asn Thr Val Asn Val Leu Ala Lys 65 70 75 80 Phe Lys Gly He Lys Ser He Glu Thr Phe Ala Val Thr He Cys Glu 85 90 95 His Phe Leu Ser Ser Phe Lys His Val He Arg Ala Gin Val Tyr Val 100 105 110 01783984\90-01 - 57 - Glu Glu Val Pro Trp Lys Arg Phe Glu Lys Asn Gly Val Lys His Val 115 120 125 His Ala Phe He Tyr Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 130 135 140 Gin He Arg Asn Gly Pro Pro Val He His Ser Gly He Lys Asp Leu 145 150 155 160 Lys Val Leu Lys Thr Thr Gin Ser Gly Phe Glu Gly Phe He Lys Asp 165 170 175 Gin Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gin 180 185 190 Val Tyr Cys Lys Trp Arg Tyr His Gin Gly Arg Asp Val Asp Phe Glu 195 200 205 Ala Thr Trp Asp Thr Val Arg Ser He Val Leu Gin Lys Phe Ala Gly 210 215 220 Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr 225 230 235 240 Asp He Gin Val Leu Thr Leu Gly Gin Val Pro Glu He Glu Asp Met 245 250 255 Glu He Ser Leu Pro Asn He His Tyr Leu Asn He Asp Met Ser Lys 260 265 270 Met Gly Leu He Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 275 280 285 Tyr Gly Arg He Thr Gly Thr Val Lys Arg Lys Leu Thr Ser Arg Leu 290 295 300 <210> 12 <211> 296 <212> PRT <213> Artificial Sequence <220> <223> PBC-DeltaNC <400> 12 Met Thr Tyr Lys Lys Asn Asp Glu Val Glu Phe Val Arg Thr Gly Tyr 1 5 10 15 Gly Lys Asp Met He Lys Val Leu His He Gin Arg Asp Gly Lys Tyr 01783984\90-01 -58- 20 25 30 His Ser lie Lys Glu Val Ala Thr Thr Val Gin Leu Thr Leu Ser Ser 35 40 45 Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp Val lie Pro Thr Asp 50 55 60 Thr lie Lys Asn Thr Val Asn Val Leu Ala Lys Phe Lys Gly lie Lys 65 70 · 75 80 Ser lie Glu Thr Phe Ala Val Thr lie Cys Glu His Phe Leu Ser Ser 85 90 95 Phe Lys His Val He Arg Ala Gin Val Tyr Val Glu Glu Val Pro Trp 100 105 110 Lys Arg Phe Glu Lys Asn Gly Val Lys His Val His Ala Phe He Tyr 115 120 125 Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu Gin He Arg Asn Gly 130 135 140 Pro Pro Val He His Ser Gly He Lys Asp Leu Lys Val Leu Lys Thr 145 150 155 160 Thr Gin Ser Gly Phe Glu Gly Phe He Lys Asp Gin Phe Thr Thr Leu 165 170 175 Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gin Val Tyr Cys Lys Trp 180 185 190 Arg Tyr His Gin Gly Arg Asp Val Asp Phe Glu Ala Thr Trp Asp Thr 195 200 205 Val Arg Ser He Val Leu Gin Lys Phe Ala Gly Pro Tyr Asp Lys Gly 210 215 220 Glu Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr Asp He Gin Val Leu 225 230 235 240 Ser Leu Ser Arg Val Pro Glu He Glu Asp Met Glu He Ser Leu Pro 245 250 255 Asn He His Tyr Phe Asn He Asp Met Ser Lys Met Gly Leu He Asn 260 265 270 01783984\90-01 - 59 - Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro Tyr Gly Lys He Thr 275 280 285 Gly Thr Val Lys Arg Lys Leu Ser 290 295 <210> 13 <211> 295 <212> PRT <213> Artificial Sequence <220> <223> PBC-DeltaNC without <400> 13 Thr Tyr Lys Lys Asn Asp Glu Val Glu Phe Val Arg Thr Gly Tyr Gly 1 5 10 15 Lys Asp Met He Lys Val Leu His He Gin Arg Asp Gly Lys Tyr His 20 25 30 Ser He Lys Glu Val Ala Thr Thr Val Gin Leu Thr Leu Ser Ser Lys 35 40 45 Lys Asp Tyr Leu His Gly Asp Asn Ser Asp Val He Pro Thr Asp Thr 50 55 60 Lys Asn Thr Val Asn Val Leu Ala Lys Phe Lys Gly He Lys Ser 70 75 80 He Glu Thr Phe Ala Val Thr He Cys Glu His Phe Leu Ser Ser Phe 85 90 95 Lys His Val He Arg Ala Gin Val Tyr Val Glu Glu Val Pro Trp Lys 100 105 110 Arg Phe Glu Lys Asn Gly Val Lys His Val His Ala Phe He Tyr Thr 115 120 125 Pro Thr Gly Thr His Phe Cys Glu Val Glu Gin He Arg Asn Gly Pro 130 135 140 Pro Val He His Ser Gly He Lys Asp Leu Lys Val Leu Lys Thr Thr 145 150 155 160 Gin Ser Gly Phe Glu Gly Phe He Lys Asp Gin Phe Thr Thr Leu Pro 165 170 175 Glu Val Lys Asp Arg Cys Phe Ala Thr Gin Val Tyr Cys Lys Trp Arg 01783984\90-01 IL 186510/3 - 60 - 180 185 190 Tyr His Gin Gly Arg Asp Val Asp Phe Glu Ala Thr Trp Asp Thr Val 195 200 205 Arg Ser lie Val Leu Gin Lys Phe Ala Gly Pro Tyr Asp Lys Gly Glu 210 215 220 Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr Asp lie Gin Val Leu 225 230 235 Leu Ser Arg Val Pro Glu lie Glu Asp Met Glu lie Ser Leu Pro Asn 245 250 255 His Tyr Phe Asn lie Asp Met Ser Lys Met Gly Leu lie Asn Lys 260 265 270 Glu Glu Val Leu Leu Pro Leu Asp Asn Pro Tyr Gly Lys lie Thr Gly 275 280 285 Thr Val Lys Arg Lys Leu 290 <210> 14 <211> 13 <212> PRT <213> Artificial Sequence <220> <223> PBC-DeltaNC (Fragment 44-56 of PBC-DeltaNC) <400> 14 Ala Thr Thr Val Gin Leu Thr Leu Ser Ser Lys Lys Asp 1 5 10 <210> 15 <211> 936 <212> DNA <213> Papio hamadryas (hamadryas baboon) <400> 15 ccagaagaaa atggccgact accataacaa ctataaaaag aatgatgaat tggagtttgt 60 ccgaactggc tatgggaagg atatggtaaa agttctccat attcagcgag atggaaaata 120 tcacagcatt aaagaggtgg caacttcagt gcaacttact ctgagttcca aaaaagatta 180 cctgcatgga gataattcag atatcatccc tacagacacc atcaagaaca cagttcatgt 240 cttggcaaag tttaagggaa tcaaaagcat agaagccttt ggtgtgaata tttgtgagta 300 ttttctttct tcttttaacc atgtaatccg agctcaagtc tacgtggaag aaatcccttg 360 01783984\90-01 IL 186510/3 61 gaagcgtctt gaaaagaatg gagttaagca tgtccatgca tttattcaca ctcccactgg 420 aacacacttc tgtgaagttg aacaactgag aagtggaccc cccgtcatta cttctggaat 480 caaagacctc aaggtcttga aaacaacaca gtctggattt gaaggtttca tcaaggacca 540 gttcaccacc ctccctgagg tgaaggaccg atgctttgcc acccaagtgt actgcaagtg 600 gcgctaccac cagtgcaggg atgtggactt cgaggctacc tggggcacca ttcgggacct 660 tgtcctggag aaatttgctg ggccctatga caaaggcgag tactcaccct ctgtgcagaa 720 gaccctctat gatatccagg tgctctccct gagccgagtt cctgagatag aagatatgga 780 aatcagcctg ccaaacattc actacttcaa tatagacatg tccaaaatgg gtctgatcaa 840 caaggaagag gtcttgctgc cattagacaa tccatatgga aaaattactg gtacagtcaa 900 gaggaagttg tcttcaagac tgtgacattg tggcca 936 <210> 16 <211> 304 <212> PRT <213> Papio hamadryas (hamadryas baboon) <400> 16 Met Ala Asp Tyr His Asn Asn Tyr Lys Lys Asn Asp Glu Leu Glu Phe 1 5 10 15 Val Arg Thr Gly Tyr Gly Lys Asp Met Val Lys Val Leu His lie Gin 20 25 30 Arg Asp Gly Lys Tyr His Ser lie Lys Glu Val Ala Thr Ser Val Gin 35 40 45 Leu Thr Leu Ser Ser Lys Lys Asp Tyr Leu His Gly Asp Asn Ser Asp 50 55 60 He He Pro Thr Asp Thr He Lys Asn Thr Val His Val Leu Ala Lys 65 70 75 80 Phe Lys Gly He Lys Ser He Glu Ala Phe Gly Val Asn He Cys Glu 85 90 95 Tyr Phe Leu Ser Ser Phe Asn His Val He Arg Ala Gin Val Tyr Val 100 105 110 Glu Glu He Pro Trp Lys Arg Leu Glu Lys Asn Gly Val Lys His Val 115 120 125 His Ala Phe He His Thr Pro Thr Gly Thr His Phe Cys Glu Val Glu 130 135 140 01783984\90-01 - 62 - 186510/4 Gin Leu Arg Ser Gly Pro Pro Val lie Thr Ser Gly lie Lys Asp Leu 145 150 155 160 Lys Val Leu Lys Thr Thr Gin Ser Gly Phe Glu Gly Phe lie Lys Asp 165 170 175 Gin Phe Thr Thr Leu Pro Glu Val Lys Asp Arg Cys Phe Ala Thr Gin 180 185 190 Val Tyr Cys Lys Trp Arg Tyr His Gin Cys Arg Asp Val Asp Phe Glu 195 200 205 Ala Thr Trp Gly Thr lie Arg Asp Leu Val Leu Glu Lys Phe Ala Gly 210 215 220 Pro Tyr Asp Lys Gly Glu Tyr Ser Pro Ser Val Gin Lys Thr Leu Tyr 225 230 235 240 Asp lie Gin Val Leu Ser Leu Ser Arg Val Pro Glu lie Glu Asp Met 245 250 255 Glu lie Ser Leu Pro Asn lie His Tyr Phe Asn lie Asp Met Ser Lys 260 265 270 Met Gly Leu lie Asn Lys Glu Glu Val Leu Leu Pro Leu Asp Asn Pro 275 280 285 Tyr Gly Lys lie Thr Gly Thr Val Lys Arg Lys Leu Ser Ser Arg Leu 290 295 300 ****** All passages of the description which are outside the scope of the claims do not constitute part of the claimed invention.
Claims (25)
1. An isolated truncated mammalian uricase which is truncated at the amino terminus or the carboxy terminus or both the amino and carboxy termini by about 1 - 6 amino acids and further comprising an amino acid substitution at position 46, said truncation and amino acid substitution being relative to a naturally occurring pig uricase having an amino acid sequence of SEQ ID NO: 11.
2. The isolated truncated mammalian uricase of claim 1 further comprising an amino terminal amino acid, wherein the amino terminal amino acid is alanine, glycine, proline, serine, or threonine.
3. The isolated truncated mammalian uricase of claim 2 wherein the amino terminal amino acid is threonine.
4. The isolated truncated mammalian uricase of claim 1 , wherein the substitution is with threonine or alanine.
5. The isolated truncated mammalian uricase of claim 3, wherein the substitution is with threonine.
6. The isolated truncated mammalian uricase of claim 5 consisting of the amino acid sequence of SEQ ID NO. 8.
7. A polyethylene glycol - uricase conjugate comprising the uricase of claim 1.
8. The conjugate of claim 7, comprising 2 to 12 polyethylene glycol molecules on each uricase subunit.
9. The conjugate of claim 7, wherein each polyethylene glycol molecule has a molecular weight between about 1 kD and 50 kD.
10. The conjugate of claim 9, wherein each polyethylene glycol molecule has a molecular weight between about 5 kD and 20 kD.
11. 1 1. A pharmaceutical composition comprising a conjugate of claim 7.
12. The composition of claim 7 for use in reducing uric acid levels in a biological fluid of a subject, wherein said composition is adapted to administration to said subject.
13. An isolated uricase comprising the amino acid sequence of SEQ ID NO. 14. IL 186510/3 - 64 -
14. The isolated truncated mammalian uricase protein of claim 1 wherein the N-terminal amino acid is methionine.
15. The uricase of claim 14 comprising the amino acid sequence of SEQ ID NO. 7.
16. An isolated nucleic acid comprising a nucleic acid sequence which encodes a protein of claim 1.
17. The isolated nucleic acid of claim 16, wherein the nucleic acid sequence is operatively linked to a heterologous promoter.
18. The nucleic acid of claim 17, wherein the promoter is the osmB promoter.
19. A nucleic acid vector comprising the nucleic acid of claim 17.
20. A host cell comprising the vector of claim 19.
21. An isolated nucleic acid comprising a nucleic acid sequence encoding a uricase comprising the amino acid sequence of SEQ ID NO. 7 or SEQ ID NO. 8.
22. The isolated nucleic acid of claim 21, wherein the nucleic acid sequence comprises SEQ ID NO. 9 or SEQ ID NO. 10.
23. The isolated nucleic acid of claim 21, wherein the nucleic acid sequence is operatively linked to a heterologous promoter.
24. The nucleic acid of claim 23, wherein the promoter is the osmB promoter.
25. A method for producing a uricase comprising the steps of culturing a host cell of claim 20 under conditions such that the nucleic acid sequence is expressed by the host cell and isolating the expressed uricase. For the Applicants RELNHOLD COHN AND PARTNERS 01783984\90-01
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PCT/US2006/013660 WO2006110819A2 (en) | 2005-04-11 | 2006-04-11 | Variant forms of urate oxidase and use thereof |
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