IL153109A - Pharmaceutical composition useful for inhibiting bone resorption in a human comprising alendronic acid or pharmaceutically acceptable salt thereof or a mixture thereof - Google Patents

Abstract

A pharmaceutical composition useful for inhibiting bone resorption in a human comprising from about 70 mg to about 140 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral administration as a unit dosage form according to a continuous schedule having a periodicity selected from once-weekly dosing and bi-weekly dosing. 3216 י' בטבת התשס" ד - January 4, 2004

Description

w n3mj N nsttin ^an oin >m many piva -nunfc > ri ninp a>_ -n A PHARMACEUTICAL COMPOSITION USEFUL FOR INHIBITING BONE RESORPTION IN A HUMAN COMPRISING ALENDRONIC ACID OR A PHARMACEUTICALLY ACCEPTABLE SALT THEREOF OR A MIXTURE THEREOF.

TITLE OF THE INVENTION A PHARMACEUTICAL COMPOSITION USEFUL FOR INHIBITING BONE RESORPTION IN A HUMAN COMPRISING ALENDRONIC ACID OR A PHARMACEUTICALLY ACCEPTABLE SALT THEREOF, OR A MIXTURE THEREOF FIELD OF THE INVENTION The present invention relates to bisphosphonates, pharmaceutically acceptable salts thereof or mixtures thereof, for use as a medicament, or for use in the manufacture of a medicament, for inhibiting bone resorption in a mammal in need thereof, the medicament being adapted for oral administration of a pharmaceutically effective amount of the bisphosphonate, pharmaceutically acceptable salt thereof or mixture thereof, in a unit dosage form, according to a continuous schedule having a periodicity from about once every three days to about once every sixteen days, such as, for example, once-weekly dosing, twice-weekly dosing, bi-weekly dosing and twice-monthly dosing. The. medicament is also adapted to minimize the occurrence of or potential for adverse gastrointestinal effects. The present invention also relates to pharmaceutical compositions and kits useful for inhibiting bone resorption in a mammal comprising the bisphosphonate, pharmaceutically acceptable salt thereof or mixture thereof, also adapted for oral administration as a unit dosage according to the aforesaid continuous schedule.

BACKGROUND OF THE INVENTION A variety of disorders in humans and other mammals involve or are associated with abnormal bone resorption. Such disorders include, but are not limited to, osteoporosis, Paget's disease, periprosthetic bone loss or osteolysis, and hypercalcemia of malignancy. The most common of these disorders is osteoporosis, which in its most frequent manifestation occurs in postmenopausal women. Osteoporosis is a systematic skeletal disease characterized by a low bone mass and microarchitectural deterioration of bone tissue, with a consequent increase in bone fragility and susceptibility to fracture. Because osteoporosis, as well as other disorders associated with bone loss, are chronic conditions, it is believed that appropriate therapy will generally require chronic treatment.

Multinucleated cells called osteoclasts are responsible for causing bone loss through a process known as bone resorption. It is well known that bisphosphonates are selective inhibitors of osteoclastic bone resorption, making these compounds important therapeutic agents in the treatment or prevention of a variety of generalized or localized bone disorders caused by or associated with abnormal bone resorption. See H. Fleisch, Bisphosphonates In Bone Disease, From the Luboruiory To The Putient, 2nd Edition, Parthenon Publishing (1995), which is incorporated by reference herein in its entirety.

At present, a great amount of preclinical and clinical data exists for the potent bisphosphonate compound alendronate. Evidence suggests that other bisphosphonates such as risedronate, tiludronate, ibandronate and zolendronate, have many properties in common with alendronate, including high potency as inhibitors of osteoclastic bone resorption. An older bisphosphonate compound, etidronate, also inhibits bone resorption. However, unlike the more potent bisphosphonates, etidronate impairs mineralization at doses used clinically, and may give rise to osteomalacia, a condition resulting in an undesirable decrease in bone mineralization. See Boyce, B.F., Foge!mari, I., Ralston, S. et al. (1984) Lancet 1(8381), pp. 821 -824 (1984), and Gibbs, C.J., Aaron, J.E.; Peacock, M. (1986) Br. Med. J. 292. pp. 1227-1229 (1986), both of which are incorporated by reference herein in their entirety.

Despite their therapeutic benefits, bisphosphonates are poorly absorbed from the gastrointestinal tract. See B.J. Gertz et al., Clinical Pharmacology of Alendronate Sodium, Osteoporosis Int., Suppl. 3:SI3-16 (1993) and B.J. Gertz et al., Studies of the oral bioavailability of alendronate, Clinical Pharmacology & Therapeutics, vol. 58, number 3, pp. 288-298 (September 1995), which are incorporated by reference herein in their entirety. Intravenous administration has been used to overcome this bioavailability problem. However, intravenous administration is costly and inconvenient, especially when the patient must be given an intravenous infusion lasting several hours on repeated occasions.

If oral administration of the bisphosphonate is desired, relatively high doses must be administered to compensate for the low bioavailability from the gastrointestinal tract. To offset this low bioavailability, it is generally recommended that the patient take the bisphosphonate on an empty stomach and fast for at least 30 minutes afterwards. However, mariy patients find the need for such fasting on a daily basis to be inconvenient. Moreover, oral administration has been associated with adverse gastrointestinal effects, especially those relating to the esophagus. See Fleisch, Id. These effects appear to be related to the irritant potential of the bisphosphonate in the esophagus, a problem which is exacerbated by the presence of refluxed gastric acid. For example, the bisphosphonate, pamidronate has been associated with esophageal ulcers. See E.G. Lufkin et al., Pamidronate: An Unrecognized Problem in Gastrointestinal Tolerability, Osteoporosis International, 4: 320-322 (1994), which is incorporated by reference herein in its entirety. Although not as common, the use of alendronate has been associated with esophagitis and/or esophageal ulcers. See P.C. De Groen, et al., Esophagitis Associated With the Use of Alendronate, New England Journal of Medicine, vol. 335, 124, pp. 1016-1021 (1996), D.O. Castell, Pill Esophagitis — The Case of Alendronate, New England Journal of Medicine, vol. 335, no. 124, pp. 1058- 1059 (1996), and U.A. Liberman et al., Esophagitis and Alendronate, New England Journal of Medicine, vol. 335, no.124, pp. 1069-1070 (1996), which are incorporated by reference herein in their entirety. The degree of adverse gastrointestinal effects of bisphosphonates has been shown to increase with increasing dose. See C.H. Chestnut et al., Alendronate Treatment of the Postmenopausal Osteoporotic Woman: Effect of Multiple Dosages on Bone Mass and Bone Remodeling, The American Journal of Medicine, vol. 99, pp. 144-152, (August 1995), which is incorporated by reference herein in its entirety. Also, these adverse esophageal effects appear to be more prevalent in patients who do not take the bisphosphonate with an adequate amount of liquid or who lie down shortly after dosing, thereby increasing the chance for esophageal reflux.

Current oral bisphosphonate therapies generally fall into two categories: (1) those therapies utilizing continuous daily treatment, and (2) those therapies utilizing a cyclic regimen of treatment and rest periods.

The continuous daily treatment regimens normally involve the chronic administration of relatively low doses of the bisphosphonate compound, with the objective of delivering the desired cumulative therapeutic dose over the course of the treatment period. However, continuous daily dosing has the potential disadvantage of causing adverse gastrointestinal effects due to the repetitive, continuous, and additive irritation to the gastrointestinal tract. Also, because bisphosphonates should be taken on an empty stomach followed by fasting and maintenance of an upright posture for at least 30 minutes, many patients find daily dosing to be burdensome. These factors can therefore interfere with patient compliance, and in severe cases even require cessation of treatment.

Cyclic treatment regiments were developed because some bisphosphonates, such as etidronate, when given daily for more than several days, have the disadvantage of actually causing a decline in bone mineralization, i.e. osteomalacia. U.S. Patent No. 4,761 ,406, to Flora et al., issued August 2, 1988, which is incorporated by reference herein in its entirety, describes a cyclic regimen developed in an attempt to minimize the decline in bone mineralization while still providing a therapeutic anti-resorptive effect. Generally, cyclic regimens are characterized as being intermittent, as opposed to continuous treatment regimens, and have both treatment periods during which the bisphosphonate is administered and nontreatment periods to permit the systemic level of the bisphosphonate to return to baseline. However, the cyclic regimens, relative to continuous dosing, appear to result in a decreased therapeutic antiresorptive efficacy. Data on lisedronalc suggests that cyclic dosing is actually less effective than continuous daily dosing for maximizing antiresoprtive bone effects. See L. Mortensen, et al., Prevention of early Postmenopausal Bone Loss By Risedronate, Journal of Bone and Mineral Research, vol. 10, supp. 1 , p. si 40 (1995), which is incorporated by reference herein in its entirety. Furthermore, these cyclic regimens do not eliminate or minimize adverse gastrointestinal effects, because such regimens typically utilize periods of multiple daily dosing. Also, the cyclic regimens are cumbersome to administer and have the disadvantage of low patient compliance, and consequently compromised therapeutic efficacy. U.S. Patent No. 5,366,965, to Strein, issued November 22, 1994, which is incorporated by reference herein in its entirety, attempts to address the problem of adverse gastrointestinal effects hy administering a polyphosphonate compound, either orally, subcutaneously, or intravenously, according to an intermittent dosing schedule having both a bone resorption inhibition period and a no-treatment rest period. However, the regimen has the disadvantage of not being continuous and regular, and requires nontreatment periods ranging from 20 to 120 days. PCT Application No. WO 95/30421, to Goodship et al., published November 16, 1995, which is incorporated by reference herein in its entirety, discloses methods for preventing prosthetic loosening and migration using various bisphosphonate compounds. Administration of a once weekly partial dose of the bisphosphonate is disclosed. However, the reference specifically fails to address the issue of adverse gastrointestinal effects or to disclose administration of larger or multiple dosages. 4 bis - 153109/1 Seedor et al., Journal of Bone & Mineral Research, Vol.6, No. 4 (1991), pp.339-346, describes an experiment in female rats, in which the rats were subcutaneously injected with various doses of alendronate. This reference does not deal with the oral administration of alendronate.

Balena et al., J. Clin. Invest., Vol. 92 (1993), pp.2577-2586, reports an experiment that was carried out in in female baboons, in which various doses of alendronate were administered intravenously. This reference does not deal with the oral administration of alendronate.

Lunar News (July 1996), pp. 23-24, refers to three problems associated with prior art alendronate treatments, namely dosing problems, high costs, and side effects. The pamphlet does not suggest any approach to the side effect problem, but speculates about the possibility of addressing dosing problems and high costs by employing 40mg or 80mg dose once/weekly. The pamphlet does not reconcile this speculation with the reports in the literature of gastrointestinal side effects in patients taking alendronate, the incidence and severity of which would obviously be expected to increase if patients took higher doses.

It is seen from current teachings that both daily and cyclic treatment regimens have shortcomings, and that there is a need for development of a dosing regimen to overcome these shortcomings.

In the present invention, it is found that the adverse gastrointestinal effects that can be associated with daily or cyclic dosing regimens can be minimized by administering the bisphosphonate at a relatively high unit dosage according to a continuous schedule having a dosing interval selected from the group consisting of once-weekly dosing, twice-weekly dosing, biweekly dosing, and twice-monthly dosing. In other words, it is found that the administration of a bisphosphonate at a high relative dosage at a low relative dosing frequency causes less adverse gastrointestinal effects, particularly esophageal effects, compared to the administration of a low relative dosage at a high relative dosing frequency. This result is surprising in view of the teachings suggesting that adverse gastrointestinal effects would be expected to increase as a function of increasing bisphosphonate dosage. Such administration in accordance with the present invention would be especially beneficial in treating patients that have been identified as suffering from or are susceptible to upper gastrointestinal disorders, e.g. gastrointestinal reflux disease (i.e. "GERD"), esophagitis, dyspepsia (i.e. heartburn), ulcers, and other related disorders. In such patients conventional bisphosphonate therapy could potentially exacerbate or induce such upper gastrointestinal disorders.

From a patient lifestyle standpoint, the unit dosage according to a continuous schedule of the present invention would also be more convenient than daily or cyclic dosing regimens. Patients would be subjected less frequently to the inconvenience of having to take the drug on an empty stomach and having to fast for at least 30 minutes after dosing. Also, patients would not need to keep track of a complex dosing regimen. The medicaments, pharmaceutical compositions and kits of the present invention are likely to have the advantage of promoting better patient compliance, which in turn can translate into better therapeutic efficacy.

It is an object of the present invention to provide a bisphosphonate or a pharmaceutically acceptable salt dicieof or a mixture thereof for use as a medicament, or for use in the manufacture of a medicament, for inhibiting bone resorption and the conditions associated therewith.

It is another object of the present invention to provide a bisphosphonate or a pharmaceutically acceptable salt thereof or a mixture thereof for use as a medicament, or for use in the preparation of a medicament, for treating abnormal bone resorption and the conditions associated therewith.

It is another object of the present invention to provide a bisphosphonate or a pharmaceutically acceptable salt thereof or a mixture thereof for use as a medicament, or for use in the manufacture of a medicament, for preventing abnormal bone resorption and the conditions associated therewith.

It is another object of the present invention to provide the above medicament in a form that is adapted for oral administration.

It is another object of the present invention to provide such medicaments for humans.

It is another object of the present invention to provide such medicaments for patients that have been identified as suffering from or are susceptible to upper gastrointestinal disorders, e.g. gastrointestinal reflux disease (i.e. "GERD"), esophagitis, dyspepsia (.i.e. heartburn), ulcers and other related disorders.

It is another object of the present invention to provide such medicaments which are also adapted for minimizing the occurrence of or potential for adverse gastrointestinal effects.

It is another object of the present invention to provide such medicaments which are adapted for oral administration, in a unit dosage form according to a continuous dosing schedule having a dosing periodicity ranging from about once every 3 days to about once every 16 days.

It is another object of the present invention to provide such medicaments for oral administration in a unit dosage form according to a continuous dosing schedule having a dosing interval selected from the group consisting of weekly dosing, twice-weekly dosing, biweekly dosing, and twice-monthly dosing.

It is another object of the present invention to provide such medicaments wherein, in use, the continuous dosing schedule is maintained until the desired therapeutic effect is achieved.

It is another object of the present invention to use such medicaments to treat or prevent abnormal bone resorption in an osteoporotic mammal, preferably an osteoporotic human.

These and other objects will become readily apparent from the detailed description which follows.

SUMMARY OF THE INVENTION The present Invention relates to pharmaceutical compositions useful for inhibiting bone resorption in a human comprising from about 70 mg to about 140 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral administration as a unit dosage form.

According to a preferred embodiment of the invention the said composition comprises a sodium, potassium, calcium, magnesium or ammonium salt of alendronic acid, the sodium salt of alendronic acid being particularly preferred.

According to a preferred embodiment of the Invention the pharmaceutical composition comprises the monosodium salt of alendronic acid. Illustrative examples of such sodium salts of alendronic acid include the hydrated monosodium salt of alendronic acid, e.g., the monosodium trihydrate salt of alendronic acid.

The pharmaceutical composition of the invention is useful for treating osteoporosis in a human in need thereof.

The pharmaceutical composition of the invention is further useful for preventing osteoporosis in a human in need thereof.

According to one preferred embodiment of the invention the pharmaceutical composition of the invention is provided in the form of a tablet.

According to another preferred embodiment of the invention the pharmaceutical composition is in the form of a capsule.

According to still anotherr preferred embodiment of the invention the pharmaceutical composition is in the form of a liquid.

Preferably, but not limitatively, the pharmaceutical composition of the invention comprises about 70 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier.

Still preferably, the pharmaceutical composition according to the Invention is adapted for oral administration as a unit dosage form, according to a continuous schedule having a periodicity of once-weekly.

All percentages and ratios used herein, unless otherwise indicated, are by weight. The invention hereof can comprise, consist of, or consist essentially of the essential as well as optional ingredients, components, and methods described herein.

BRIEF DESCRIPTION OF THE FIGURES FIG. 1 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed immediately after infusion of the last of five separate dosages of 50 mL of simulated gastric juice administered on five consecutive days. - 8 - 12 - FIG. 2 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed immediately after infusion of the last five separate dosages of 50 mL of 0.20 mg/mL alendronate in simulated gastric juice administered on five consecutive days. FIG. 3 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed 24 hours after infusion with a single dosage of 50 mL of 0.80 mg mL alendronate in simulated gastric juice.

FIG. 4 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed 7 days after infusion with a single dosage of 50 mL of 0.80 mg mL alendronate in simulated gastric juice.

FIG. 5 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed 7 days after infusion of the last of 4 separate dosages of 50 mL of 0.80 mg/mL alendronate in simulated gastric juice administered once per week, i.e. once every 7 days.

FIG. 6 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed 4 days after infusion of the last of 8 separate dosages of 50 mL of 0.40 mg/ml alendronate in simulated gastric juice administered twice per week, i.e. once every 3-4 days.

FIG. 7 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed immediately after infusion of the last of five separate dosages of 50 mL of 0.20 mg mL risedronate in simulated gastric juice administered on five consecutive days.

FIG. 8 is a photomicrograph (total magnification 270X) of canine esophagus tissue (paraffin embedded and stained with hematoxylin and eosin) from an animal sacrificed immediately after infusion of the last of five separate dosages of 50 mL of 4.0 mg/mL tiludronate in simulated gastric juice administered on five consecutive days.

DESCRIPTION OF THE INVENTION The present invention relates to a bisphosphonate or a pharmaceutically acceptable salt thereof or a mixture thereof, for use as a medicament, or for use in the manufacture of a medicament, for inhibiting bone resorption in a mammal, preferably in a human, in need thereof, while minimizing the occurrence of or potential for adverse gastrointestinal effects. The present invention also relates to pharmaceutical compositions and kits comprising the bisphosphonate, a pharmaceutically acceptable salt or ester thereof or a mixture thereof, for inhibiting bone resorption in a mammal, preferably in a human in need thereof, while minimizing the occurrence of or potential for adverse gastrointestinal effects. The present invention relates to all of the aforesaid medicament, pharmaceutical composition and kit for treating or preventing abnormal bone resorption in a mammal, preferably in a human, in need of such treatment or prevention. The medicament, pharmaceutical composition, and kit of the present invention are adapted for oral administration, in a unit dosage form comprising a pharmaceutically effective amount of a bisphosphonate or a pharmaceutically acceptable salt or ester thereof, according to a continuous schedule having a periodicity from about once every 3 days to about once every 16 days. In other embodiments, the continuous schedule has a dosing interval selected from the group consisting of once-weekly dosing, twice-weekly dosing, biweekly dosing, and twice-monthly dosing. Typically, the continuous dosing schedule is maintained until the desired therapeutic effect is achieved.

In accordance with the present invention there are utilized higher unit dosages of the bisphosphonate or a pharmaceutically acceptable salt or ester thereof, or a mixture thereof at each dosing point than has heretofore been typically utilized, yet because of the dosing schedule chosen, the potential for adverse gastrointestinal effects are minimized. Moreover, the unit dosage according to the continuous schedule is more convenient because the disadvantages associated with daily dosing are minimized.

The medicament, pharmaceutical composition and kit of the present invention are generally intended for administration to mammals in need of bisphosphonate therapy. Preferably, the mammals are human patients, particularly human patients in need of inhibiting bone resorption, such as patients in need of treating or preventing abnormal bone resorption.

The medicament, pharmaceutical composition and kit of the present invention are especially useful for the therapy of human patients who have been identified as suffering from or are susceptible to upper gastrointestinal disorders, e.g. GERD, esophagitis, dyspepsia, ulcers, etc. In such patients conventional bisphosphonate therapy could potentially exacerbate or induce such upper gastrointestinal disorders.

The term "pharmaceutically effective amount", as used herein, means that amount of the bisphosphonate compound, that will elicit the desired therapeutic effect or response when administered in accordance with the desired treatment regimen. A preferred pharmaceutically effective amount of the bisphosphonate is a bone resorption inhibiting amount.

The term "minimize the occurrence of or potential for adverse gastrointestinal effects", as used herein, means reducing, preventing, decreasing, or lessening the occurrence of or the potential for incurring unwanted side effects in the gastrointestinal tract, i.e. the esophagus, stomach, intestines, and rectum, particularly the upper gastrointestinal tract, i.e. the esophagus and stomach. Nonlimiting adverse gastrointestinal effects include, but are not limited to GERD, esophagitis, dyspepsia, ulcers, esophageal perforation, abdominal pain, and constipation.

The term "abnormal bone resorption", as used herein means a degree of bone resorption that exceeds the degree of bone formation, either locally, or in the skeleton as a whole. Alternatively, "abnormal bone resorption" can be associated with the formation of bone having an abnormal structure.

The term "bone resorption inhibiting", as used herein, means treating or preventing bone resorption by the direct or indirect alteration of osteoclast formation or activity. Inhibition of bone resorption refers to treatment or prevention of bone loss, especially the inhibition of removal of existing bone-either from the mineral phase and/or the organic matrix phase, through direct or indirect alteration of osteoclast formation or activity.

The terms "continuous schedule" or "continuous dosing schedule", as used herein, mean that the dosing regimen is repeated until the desired therapeutic effect is achieved. The continuous schedule or continuous dosing schedule is distinguished from cyclical or intermittent administration.

The term "until the desired therapeutic effect is achieved", as used herein, means that the bisphosphonate or a pharmaceutically acceptable salt or ester thereof, or a mixture thereof is the active ingredient in the medicament, pharmaceutical g.\e20Ol\96l 5VJU33535spec.doc Μ/04/OI/ir/gg/sZ· composition or kit of the invention, which is adapted for continuous administration, according to the dosing schedule chosen, up to the time that the clinical or medical effect sought for the disease or condition is observed by the clinician or researcher. The continuous administration is until the desired change in bone mass or structure is observed. In such instances, achieving an increase in bone mass or a replacement of abnormal bone structure with more normal bone structure are the desired objectives. When intended for prevention of abnormal bone resorption, the continuous administration of the medicament, pharmaceutical composition or kit is for as long as necessary to prevent the undesired condition. In such instances, maintenance of bone mass density is often the objective. Nonlimiting examples of administration periods can range from about 2 weeks to the remaining lifespan of the mammal. For humans, administration periods can range from about 2 weeks to the remaining lifespan of the human, preferably from about 2 weeks to about 20 years, more preferably from about 1 month to about 20 years, more preferably from about 6 months to about 10 years, and most preferably from about 1 year to about 10 years.

Medicaments, Pharmaceutical Compositions And Kits Of The Present Invention The present invention comprises a bisphosphonate or a pharmaceutically acceptable salt or ester thereof, or a mixture thereof, for use as a medicament, or for use in the manufacture of a medicament, or as the active ingredient in a pharmaceutical composition, or a kit, all for inhibiting bone resorption in mammals. The present invention also comprises all of the aforesaid for the purposes of treating or for preventing abnormal bone resorption in mammals, preferably in humans.

The medicaments, pharmaceutical compositions and kits of the present invention do not have the disadvantages of current methods and associated medicaments, pharmaceutical compositions and kits for treatment which can cause or increase the potential for adverse gastrointestinal effects or which require cumbersome, irregular or complicated dosing regimens.

The medicaments, pharmaceutical compositions and kits of the present invention are adapted for a continuous dosing schedule whereby a unit dosage of the bisphosphonate or a pharmaceutically acceptable salt or ester thereof, or a mixture thereof, is regularly administered according to a dosing interval, which may be from about once every 3 days to about once every 16 days. Embodiments of the dosing schedule include a dosing interval selected from the group consisting of once-weekly dosing, twice-weekly dosing, biweekly dosing, and twice-monthly dosing.

By once-weekly dosing is meant that a unit dosage of the bisphosphonate is administered once a week, i.e. one time during a seven-day period, preferably on the same day of each week. In the once-weekly dosing regimen, the unit dosage is generally administered about every seven days. A nonlimiting example of a once-weekly dosing regimen would entail the administration of a unit dosage of the bisphosphonate every Sunday. It is preferred that the unit dosage is not administered on consecutive days, but the once-weekly dosing regimen can include a dosing regimen in which unit dosages are administered on two consecutive days falling within two different weekly periods.

By twice-weekly dosing is meant that a unit dosage of the bisphosphonate is administered twice a week, i.e. two times during a seven day period, preferably on the same two days of each weekly period. In the twice-weekly dosing regimen, each unit dosage is generally administered about every three to four days. A nonlimiting example of a twice-weekly dosing regimen would entail the administration of a unit dosage of the bisphosphonate every Sunday and Wednesday. It is preferred that the unit dosages are not administered on the same or consecutive days, but the twice-weekly dosing regimen can include a dosing regimen in which unit dosages are administered on two consecutive days within a weekly period or different weekly periods.

By biweekly is meant that a unit dosage of the bisphosphonate is administered once during a two week period, i.e. one time during a fourteen day period, preferably on the same day during each two week period. In the twice-weekly dosing regimen, each unit dosage is generally administeied about every fourteen days. A nonlimiting example of a biweekly dosing regimen would entail the administration of a unit dosage of the bisphosphonate every other Sunday. It is preferred that the unit dosage is not administered on consecutive days, but the biweekly dosing regimen can include a dosing regimen in which the unit dosage is administered on two consecutive days within two different biweekly periods.

By twice-monthly dosing is meant that a unit dosage of the bisphosphonate is administered twice, i.e. two times, during a monthly calendar period. With the twice-monthly regimen, the doses are preferably given on the same two dates of each month. In the twice-monthly dosing regimen, each unit dosage is generally administered about every fourteen to sixteen days. A nonlimiting example of a biweekly dosing regimen would entail dosing on or about the first of the month and on or about the fifteenth, i.e. the midway point, of the month. It is preferred that the unit dosages are not administered on the same or consecutive days but the twice-monthly dosing regimen can include a dosing regimen in which the unit dosages are administered on two consecutive days within a monthly period, or different monthly periods. The twice-monthly regimen is defined herein as being distinct from, and not encompassing, the biweekly dosing regimen because the two regimens have a different periodicity and result in the administration of different numbers of dosages over long periods of time. For example, over a one year period, a total of about twenty four dosages would be administered according to the twice-monthly regimen (because there are twelve calendar months in a year), whereas a total of about twenty six dosages would be administered according to the biweekly dosing regimen (because there are about fifty-two weeks in a year).

In further embodiments or descriptions of the present invention, the unit dosage is given with a periodicity ranging from about once every 3 days to about once every 16 days.

The medicaments, pharmaceutical compositions and kits of the present invention are useful for inhibiting bone resorption and for treating and preventing abnormal bone resorption and conditions associated therewith. Such conditions include both generalized and localized bone loss. Also, the creation of bone having an abnormal structure, as in Paget's disease, can be associated with abnormal bone resorption. The term "generalized bone loss" means bone loss at multiple skeletal sites or throughout the skeletal system. The term "localized bone loss" means bone loss at one or more specific defined skeletal sites.

Generalized bone loss is often associated with osteoporosis. Osteoporosis is most common in post-menopausal women, wherein estrogen production has been greatly diminished. However, osteoporosis can also be steroid-induced and has been observed in males due to age. Osteoporosis can be induced by disease, e.g; rheumatoid arthritis, it can be induced by secondary causes, e.g. glucocorticoid therapy, or it can come about with no identifiable cause, i.e. idiopathic osteoporosis. In the present invention, preferred medicaments, pharmaceutical compositions and kits include those for the treatment or prevention of abnormal bone resorption in osteoporotic humans.

Localized bone loss has been associated with periodontal disease, with bone fractures, and with periprosthetic osteolysis (in other words where bone resorption has occurred in proximity to a prosthetic implant).

Generalized or localized bone loss can occur from disuse, which is often a problem for those confined to a bed or a wheelchair, or for those who have an immobilized limb set in a cast or in traction.

The medicaments, pharmaceutical compositions and kits of the present invention are useful for treating and/or preventing the following conditions or disease states: osteoporosis, which can include post-menopausal osteoporosis, steroid-induced osteoporosis, male osteoporosis, disease-induced osteoporosis, idiopathic osteoporosis; Paget's disease; abnormally increased bone turnover; periodontal disease; localized bone loss associated with periprosthetic osteolysis; and bone fractures.

The medicaments, pharmaceutical compositions and kits of the present invention are intended to specifically exclude those for the treatment and/or prevention of prosthesis loosening and prosthesis migration in mammals as described in PCT application WO 95/30421 , to Goodship et al., published November 16, 1995, which is incorporated by reference herein in its entirety.

Bisphosphonates The medicaments, pharmaceutical compositions and kits of the present invention comprise a bisphosphonate, or a pharmaceutically acceptable salt or ester thereof. The bisphosphonates of the present invention correspond to the chemical formula: - 15d -:\e2001\ 6l 5U\!33535s {a).d<κ 05/M/01/ir/ggft2· A-C-X I P03H2 wherein A and X are independently selected from the group consisting of H, OH, halogen, H2, SH, phenyl, C1-C30 alkyl, C1-C30 ' substituted alkyl, Cl-ClO alkyl or dialkyl substituted NH2, Cl-ClO alkoxy, Cl-ClO alkyl or phenyl substituted thio, Cl-ClO alkyl substituted phenyl, pyridyl, furanyl, pyrrolidinyl, imidazoriyi; and benzyl.

In the foregoing chemical formula, the alkyl groups can be straight, branched, or cyclic* provided sufficient atoms are selected for the chemical formula. The C1-C30 substituted alkyl can include a wide variety of substituents, nonlimiting examples which include those selected from the group consisting of phenyl, pyridyl, furanyl, pyrroh'dinyl, imidazonyl, H2. Cl-ClO alkyl or dialkyl substituted NH2, OH, SH, and Cl-ClO alkoxy.

In the foregoing chemical formula, A can include X and X can include A such, that the two moieties can form part of the same cyclic structure.

The foregoing chemical formula is also intended to encompass complex carbocyclic, aromatic and hetero atom structures for the A and/or X substituents, nonlimiting examples of which include naphthyl, quinolyl, isoquinolyl, adamantyl, and chlorophenylthio.

Preferred structures are those in which A is selected from . the group consisting of H, OH, and halogen, and X is selected from the group consisting of C1-C30 alkyl, C1-C30 substituted alkyl, halogen, and Cl-ClO alkyl or phenyl substituted thio.

More preferred structures are those in which A is selected from the group consisting of H, OH, and CI, and X is selected from the group consisting of C1-C30 alkyl, C1-C30 substituted alkyl, CI, and chlorophenylthio.

-IS- Most preferred is when A is OH and X is a 3-aminopropyl moiety, so that the resulting compound is a 4-amino-l,-hydroxybutylidene-l,l-bisphosphonate, i.e. alendronate.

Pharmaceutically acceptable salts and derivatives of the bisphosphonates are also useful herein. Nonlimiting examples of salts include those selected from the group consisting alkali metal, alkaline metal, ammonium, and mono-, di, tri-, or tetra-Cl-C30-alkyl-substituted ammonium. Preferred salts are those selected from the group consisting of sodium, potassium, calcium, magnesium, and ammonium salts. Nonlimiting examples of derivatives include those selected from the group consisting of esters, hydrates, and amides.

"Pharmaceutically acceptable" as used herein means that the salts and derivatives of the bisphosphonates have the same general pharmacological properties as the free acid form from which they are derived and are acceptable from a toxicity viewpoint.

It should be noted that the terms "bisphosphonate" and "bisphosphonates", as used herein in referring to the therapeutic agents of the present invention are meant to also encompass diphosphonates, hiphosphonic acids, and diphosphonic acids, as well as salts and derivatives of these materials. The use of a specific nomenclature in referring to the bisphosphonate or bisphosphonates is not meant to limit the scope of the present invention, unless specifically indicated. Because of the mixed nomenclature currently in use by those or ordinary skill in the art, reference to a specific weight or percentage of a bisphosphonate compound in the present invention is on an acid active weight basis, unless indicated otherwise herein. For example, the phrase "about 70 mg of a bone resorption inhibiting bisphosphonate selected from the group consisting of alendronate, pharmaceutically acceptable salts thereof, and mixtures thereof, on an alandronic acid active weight basis" means that the amount of the bisphosphonate compound selected is calculated based on 70 mg of alendronic acid.

Nonlimiting examples of bisphosphonates useful herein include the following: Alendronic acid, 4-arcdno-l-hydroxybutyHdene-l,l- bisphosphonic acid.

Alendronate (also known as alendronate sodium or monosodium trihydrate), 4-amino-l -hydroxy butylidene-l ,l-bisphosphonic acid monosodium trihydrate.

Alendronic acid and alendronate are described in U.S. Patents 4,922,007, to Kieczykowski et al., issued May 1 , 1 90, its corresponding IL 94612, and 5,01 ,651 , to Kieczykowski, issued May 28, 1991 , all of which are incorporated by reference herein in their entirety.

Alendronic acid and other bisphosphonates are also described in US patent 4,621 ,077, to Rosini et al., issued November 4, 1986, which is incorporated by reference herein in its entirety.

Cycloheptylaminomcthylcnc-l ,l-bisphosphonic acid, YM 175, Yamanouchi (cimadronate), as described in U.S. Patent 4,970,335, to Isomura et al., issued November 13, 1990, which is incorporated by reference herein in its entirety. 1 , l -dichloromethylene-l ,l -diphosphonic acid (clodronic acid), and the disodium salt (clodronate, Procter and Gamble) are described in Belgium Patent 672,205 (1966) and J. Org. Chem 32, 4111 (1967), both of which are incorporated by reference herein in their entirety ] 1 -hy droxy-3-( 1 -pyrrolidiny l)-propylidene- 1 , 1 -bisphosphonic acid (EB- 1053). 1 -hydroxy ethane- 1, 1-diphosphonic acid (etidronic acid). 1 -hydroxy-3-(N-methyl-N-pentylamino)propylidene- 1 , 1 -bisphosphonic acid, also known as BM-210955, Boehringer-Mannheim (ibandronate), is described in U.S. Patent No. 4,927,814, issued May 22, 1990 and its corresponding IL 83148, which are incorporated by reference herein in their entirety. 6-amino-l -hydroxy hexylidene- 1 , 1 -bisphosphonic acid (neridronate). 3-(dimethylamino)- 1 -hydroxypropy lidene- 1 , 1 -bisphosphonic acid (olpadronate). 3-amino-hydroxypropy lidene- 1 , 1 -bisphosphonic acid (pamidronate). [2-(2-pyridinyl)ethylidene]- 1 ,1 -bisphosphonic acid (piridronate) is described in U.S. Patent No. 4,761 ,406, which is incorporated by reference in its entirety. 1 -hydroxy-2-(3-pyridiny l)-ethylidene- 1 , 1 -bisphosphonic acid (risedronate). (4-chlorophenyl)thiomethane- 1 , 1 -diphosphonic acid (tiludronate) as described in U.S. Patent 4,876,248, to Breliere et al., issued October 24, 1989 and its corresponding IL 69374, which are incorporated by reference herein in their entirety. 18 1 -h droxy-2-( 1 H-imidazol- 1 -yl)ethy lidene- 1 , 1 -bisphosphonic acid (zoiendronatc).

Preferred are bisphosphonates selected from the group consisting of alendronate, cimadronate, clodronate, tiludronate "etidronate, ibandronate, risedronate, piridronate, pamidronate, zolendronate, pharmaceutically acceptable salts or esters thereof, and mixtures thereof.

Pharmaceutical Compositions Compositions useful in the present invention comprise a pharmaceutically effective amount of a bisphosphonate or a pharmaceutically acceptable salt or ester thereof, or a mixture thereof. The bisphosphonate is typically administered in admixture with suitable pharmaceutical diluents, excipients, or carriers, collectively referred to herein as "carrier materials", suitably selected with respect to oral administration, i.e. tablets, capsules, elixirs, syrups, effervescent compositions, powders, and the like, and consistent with conventional pharmaceutical practices. For example, for oral administration in the form of a tablet, capsule, or powder, the active ingredient enn be combined with an oral, non-toxic, pharmaceutically acceptable inert carrier such as lactose, starch, sucrose, glucose, methylcellulose, magnesium stearate, mannitol, sorbitol, croscarmellose sodium and the like; for oral administration in liquid form, e.g., elixirs and syrups, effervescent compositions, the oral drug components can be combined with any oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents, buffers, coatings and coloring agents can also be incorporated. Suitable binders can include starch, gelatin, natural sugars such as glucose, anhydrous 19 lactose, free-flow lactose, beta-lactose, and corn sweeteners, natttral and synthetic gums, such as acacia, guar, tragacanth or sodium alginate, carboxymethyl cellulose, polyethylene glycol, waxes, and the like.

Lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. A particularly preferred tablet formulation for alendronate mondsodium trihydrate is that described in U.S. Patent No. 5,358,941, to Bechard et al, issued October 25, 1994, which is incorporated by reference herein in its entirety. The compounds used in the present method can also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxylpropyl-methacrylamide, and the like.

The precise dosage of the bisphonate will vary with the dosing schedule, the oral potency of the particular bisphosphonate chosen, the age, size, sex and condition of the mammal or human, the nature and severity of the disorder to be treated, and other relevant medical and physical factors. Thus, a precise pharmaceutically effective amount cannot be specified in advance and can be readily determined by the caregiver or clirdcian. Appropriate amounts can be determined by routine experimentation from animal models and human clinical studies. Generally, an appropriate amount of bisphosphonate is chosen to obtain a bone resorption inhibiting effect, i.e. a bone resorption inhibiting amount of the bisphosphonate is administered. For humans, an effective oral dose of bisphosphonate is typically from about 1.5 to about 6000 g/k body weight and preferably about 10 to about 2000 g kg of body weight For human oral compositions comprising alendronate, pharmaceutically acceptable salts thereof, or pharmaceutically acceptable derivatives thereof, a unit dosage typically comprises from about 8.75 mg to about 140 mg of the alendronate compound, on an alendronic acid active weight basis.

For once-weekly dosing, an oral unit dosage comprises from about 17.5 mg to about 70 mg of the alendronate compound, on an alendronic acid active weight basis. Examples of weekly oral dosages include a unit dosage which is useful for osteoporosis prevention comprising about 35 mg of the alendronate compound, and a unit dosage which is useful for treating osteoporosis comprising about 70 mg of the alendronate compound.

For twice-weekly dosing, an oral unit dosage comprises from about 8.75 mg to about 35 mg of the alendronate compound, on an alendronic acid active weight basis. Examples of twice-weekly oral dosages include a unit dosage which is useful for osteoporosis prevention comprising about 17.5 mg of the alendronate compound, and a unit dosage which is useful for osteoporosis treatment, comprising 'about 35 mg of the alendronate compound.

For biweekly or twice-monthly dosing, an oral unit dosage comprises from about 35 mg to about 140 mg of the alendronate compound, on an alendronic acid active weight basis. Examples of biweekly or twice-monthly oral dosages include a unit dosage which is useful for osteoporosis prevention comprising about 70 mg of the alendronate compound, and a unit dosage which is useful for osteoporosis treatment, comprising about 140 mg of the alendronate compound.

Nonlimiting examples of oral compositions comprising alendronate, as well as other bisphosphonates, are illustrated in the Examples, below.

Sequential Administration Of Histamine H2 Receptor Blockers And Or Proton Punvp Inhibitors With Bisnhosnhonates In further embodiments, the methods and compositions of the present invention can also comprise a histamine H2 receptor blocker (i.e. antagonist) and/or a proton pump inhibitor. Histamine H2 receptor blockers and proton pump inhibitors are well known therapeutic agents for increasing gastric pH. See L.J. Hixson, et al., Current Trends in the Pharmacotherapy for Peptic Ulcer Disease, Arch. Intern. Med., vol. 152, pp. 726-732 (April 1992), which is incorporated by reference herein in its entirety. It is found in the present invention that the sequential oral administration of a histamine H2 receptor blocker and/or a proton pump inhibitor, followed by a bisphosphonate can help to further minimize adverse gastrointestinal effects. In these embodiments, the histamine H2 receptor blocker and/or proton pump inhibitor is administered from about 30 minutes to about 24 hours prior to the administration of the bisphosphonate. In more preferred embodiments, the histamine H2 recep or blocker and/or proton pump inhibitor is administered about 30 minutes to about 12 hours prior to the administration of the bisphosphonate.

The dosage of the histamine H2 receptor blocker and/or proton.pump inhibitor will depend upon the particular compound selected and factors associated with the mammal to be treated, i.e. size, health, etc.

Nonlimiting examples of histamine H2 receptor blockers and/or proton pump inhibitors include those selected from the group consisting of cimetidine, famotidine, nizatidine, ranitidine, omeprazole and lansoprazole.

Treatment Kits In further embodiments, the present invention relates to a kit for conveniently and effectively enabling the oral administration of the medicament or the pharmaceutical composition of the invention, in a unit dosage form according to the continuous schedule. Such kits are especially suited for the delivery of solid oral forms such as tablets or capsules. Such a kit preferably includes a number of unit dosages. Such kits can include a card having the dosages oriented in the order of their intended use. An example of such a kit is a "blister pack". Blister packs are well known in the packaging industry and are widely used for packaging pharmaceutical unit dosage forms. If desired, a memory aid can be provided, for example in the form of numbers, letters, or other markings or with a calendar insert, designating the days in the treatment schedule in which the dosages can be administered. Alternatively, placebo dosages, or calcium or dietary supplements, either in a form similar to or distinct from the bisphosphonate dosages, can be included to provide a kit in which a dosage is taken every day. In those embodiments including a histamine H2 receptor and/or proton pump inhibitor, these agents can be included as part of the kit. g:\e2001\9615\3\l33535specl.doc 0 /M/OI/gg* 22 EXAMPLES The following examples further describe and demonstrate embodiments within the scope of the present invention. The examples are given solely for the purpose of illustration and are not to be construed as limitations of the present invention as many variations thereof are possible without departing from the spirit and scope of the invention.

EXAMPLE 1 Esophageal Irritation Potential The esophageal irritation potential of the bisphosphonates is evaluated using a dog model.

The experiments demonstrate the relative irritation potential of the following dosing regimens: placebo (Group 1), a single high concentration dosage of alendronate monosodium trihydrate (Group 2), a low concentration dosage of alendronate monosodium trihydrate administered for five consecutive days (Groups 3 and 4), a high concentration dosage of alendronate monosodium trihydrate administered once per week for four weeks (Group 5), a mid-range concentration dosage of alendronate monosodium trihydrate administered twice per week for four weeks (Group 6), a low dosage of risedronate sodium administered for five consecutive days (Group 7), and a low dosage of tiludronate disodium administered for five consecutive days (Group 8).

The following solutions are prepared: (1) simulated gastric juice (pH about 2), i.e. the control solution. (2) simulated gastric juice (pH about 2) containing about 0.20 mg/mli of alendronate monosodium trihydrate on an alendronic acid active basis. (3) simulated gastric juice (pH about 2) containing about 0.80 mg/mL of alendronate monosodium trihydrate on an alendronic acid active basis. ; (4) simulated gastric juice (pH about 2) containing about 0.40 : mg/mL of alendronate monosodium trihydrate on an alendronic acid active basis. ; (5) simulated gastric juice (pH about 2) containing about 0.20 mg mL of risedronate sodium on a risedronic acid active basis. (8) simulated gastric juice (pH about 2) containing about 4.0 mg/mL of tiludronate disodium on a tiludronic acid active basis.

Tn. inula ted gastric juice is prepared by dissolving about 960 mg of pepsin (L-585,228000B003, Fisher Chemical) in about 147 mL of 0.90 (wt %) NaCl (aqueous), adding about 3mL of 1.0 M HC1 (aqueous), and adjusting the volume to about 300 mL with deiomzed water. The pH of the resulting solution is measured and if necessary is adjusted to about 2 using 1.0 M HC1 (aqueous) or 1.0 M NaOH (aqueous).

The animals used in the experiments are anesthetized and adininistered about 50 mL of the appropriate solution over about 30 minutes by infusion into the esophagus using an infusion pump and a rubber catheter. The following treatment experiments are run: Group 1: This control group contains four animals. Each animal is acb-oinistered a dosage of about 59 mL of simulated gastric juice [solution (1)] on each of five consecutive days. The animals are sacrificed immediately after the last dose is administered.

Group 2: This group contains four animals. Each animal is administered a dosage of about 50 mL of simulated gastric juice containing about 0.20 mg/mL of alendronate [solution (2)] on each of five consecutive days. The animals are sacrificed immediately after the last dose is administered.

Group 3: This group contains five animals. Each animal is administered a. dosage of about 50 mL of simulated gastric juice containing about 0.80 mg mL of alendronate [solution (3)1 on a single treatment day. The animals are sacrificed about 24 hours after the dose is administered.

Group 4: This group contains five animals. Each animal is administered a dosage of about 50 ml. of simulated gastric juice containing about 0.80 mg/mL of alendronate [solution (3)] on a single treatment day. The animals are sacrificed about 7 days after the dose is administered.

Group 5: This group contains six animals. Each animal is administered a dosage of about 50 mli of simulated gastric juice containing about 0.80 mg/mL of alendronate [solution (3)] once per week, i.e. every seven days, for four weeks. The animals are administered a total of four dosages. The animals are sacrificed about 7 days after the last dose is administered.

Group 6: This group contains six animals. Each animal is adrninistered a dosage of about 50 mL of simulated gastric juice containing about 0.40 mg/mL of alendronate [solution (4)] twice per week, i.e. every three to four days, for four weeks. The animals are administered a total of eight dosages. The animals are sacrificed about four days after the last dose is administered.

Group 7: This group contains eighfanimals. Each animal is adniinistered a dosage of about 50 mL of simulated gastric juice containing about 0.20 mg/mL of risedronate [solution (5)] on each of five consecutive days. The animals are sacrificed immediately after the last dose is administered.

Group 8: This group contains four animals. Each animal is administered a dosage of about 50 mL of simulated gastric juice containing about 4.0 mg/mL of tiludronate [solution (6)] on each of five consecutive days. The animals are sacrificed immediately after the last dose is adnnnistered.

The esophagus from each sacrificed animal is removed and prepared for histopathology using standard techniques by embedding the tissue in paraffin, staining with hematoxylin and eosin. The sections are examined microscopically. The histopathology results are summarized in Table 1.

For the Group 1 animals (control group), the photomicrographs show that the esophagus is normal with an intact epithelium and absence of inflammatory cells in the submucosa. FIG. 1 is a representative photomicrograph from a Group 1 animal.

For the Group 2 animals, the photomicrographs show that 'the esophagus exhibits deep ulceration of the epithelial surface and marked submucosal inflammation and vacuolation. FIG. 2 is a representative photomicrograph from a Group 2 animal.

For the Group 3 animals, the photomicrographs show that the esophagus has an intact epithelial surface with very slight submucosal inflammation and vacuolation. FIG. 3 is a representative photomicrograph from a Group 3 animal.

For the Group 4 animals, the photomicrographs show that the esosphagus has an intact epithelium with either minimal inflammation (two of the five animals) or no inflammation (three of the five animals) and no vacuolation. FIG. 4 is a representative photomicrograph from a Group 4 animal exhibiting minimal inflammation.

For the Group 5 animals, the photomicrographs show that the esophagus is normal with an intact epithelium and absence of inflammatory cells in the submucosa. FIG. 5 is a representative photomicrograph from a Group 5 animal.

For the Group 6 animals, the photomicrographs show that the esophagus exhibits deep ulceration of the epithelial surface and marked submucosal inflammation and vacuolation. FIG. 6 is a representative photomicrograph from a Group 6 animal.

For the Grou 7 animals, the photomicrographs show that the esophagus exhibits deep ulceration of the epithelial surface and marked submucosal inflammation and vacuolation. FIG. 7 is a representative photomicrograph from a Group 7 animal.

For the Group 8 animals, the photomicrographs show that the esophagus exhibits slight ulceration of the epithelial surface and slight submucosal inflammation and variolation. FIG. 8 is a representative photomicrograph from a Group 8 animal.

These experiments demonstrate that considerably less esophageal irritation (comparable to control Group l)is observed from the administration of a single high concentration dosage of alendronate (Groups 3 and 4) versus adininistration of low concentration dosages on consecutive days (Group 2). These experiments also demonstrate . 'consideraly less esophageal irritation is observed from the administration of a single high concentration of alendronate on a weekly basis (Group 5) or twice-weekly basis (Group 6) versus acbmnistration of low concentration dosages on consecutive days (Group 2). These experiments also demonstrate that when other bisphosphonates such as risedronate (Group 7) or tiludronate (Group 8) are administered at low dosages on consecutive days that the esophageal irritation potential is high.

Table 1. 6 Alendronate 2X immediate Deep ulceration of (n=6) 0.40 weekly ly after epithelial surface. for 4 last Marked submucosal weeks dosing inflammation and vacuolation. 7 Risedronate IX daily immediate Deep ulceration of (n=8) 0.20 for 5 days ly after epithelial surface (4 last of 8 animals). dosing Marked submucosal inflammation and vacuolation. 8 Tiludronate IX daily 24 hours Slight submucosal (n=4) 4.0 for 5 days after last inflammation and dosing vacuolation (3 of 4 animals, including 1 of these animals with slight ulceration).

EXAMPLE 2 Once-weekly dosing regimen.

Treatment of osteoporosis.

Alendronate tablets or liquid formulations containing about 70 mg of alendronate, on an alendronic acid active basis, are prepared, (see EXAMPLES 7 and 8). The tablets or liquid formulations are orally administered to a human patient once-weekly, i.e. preferably about once every seven days (for example, every Sunday), for a period of at least one year. This method of administration is useful and convenient for treating osteoporosis and for minimizing adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance.

Prevention of osteoporosis.

Alendronate tablets or liquid formulations containing about 35 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and8). The tablets or liquid formulations are orally administered to a human patient once-weekly, i.e. preferably about once every seven days (for example, every Sunday), for a period of at least one year. This method of administration is useful and convenient for preventing osteoporosis and for minimiring adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance.

EXAMPLE 3 Twice- weekly dosing regimen.

Treatment of osteoporosis.

Alendronate tablets or liquid formulations containing about 35 mg of alendronate, on an alendronic acid active basis, are prepared (see.EXAMPLES 7 and 8). The tablets or liquid formulations are orally administered to a human patient twice- weekly, preferably about once every three or four days (for example, every Sunday and Wednesday), for a period of at least one year. This method of administration is useful and convenient for treating osteoporosis and for minimizing adverse gastrointestinal effects, particularly advers*e esophageal effects. This method is also useful for improving patient acceptance and compliance.

Prevention of osteoporosis.

Alendronate tablets or liquid formulations containing about 17.5 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and8). The tablets or liquid. formulations are orall administered to a human patient twice-weekly, preferably about once every three or four days (for example, every Sunday and Wednesday), for a period of at least one year. This method of administration is useful and convenient for preventing osteoporosis and for minimizing adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance.

EXAMPLE 4 Biweekly dosing regimen Treatment of osteoporosis.

Alendronate tablets or liquid formulations containing about 140 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and 8). The tablets or liquid formulations are orally administered to a human patient biweekly, i.e. preferably about once every fourteen days (for example, on alternate Sundays), for a period of at least one year. This method of administration is useful and convenient for treating osteoporosis and for minimizing adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance.

Prevention of osteoporosis.

Alendronate tablets or liquid formulations containing about 70 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and 8). The tablets or liquid formulations are orally administered to a human patient biweekly,_i.e. preferably about once every fourteen days (for example, on alternate Sundays), for a period of at least one year. This method of administration is useful and convenient for preventing osteoporosis and for imriimizihg adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance. ' • EXAMPLE 5 Twice-monthly dosing regimen.

Treatment of osteoporosis.

Alendronate tablets or liquid formulations containing about 140 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and 8). The tablets or liquid' formulations are orally administered to a human twice-monthly, i.e. preferably about once every fourteen to sixteen days (for example, on about the first and fifteenth of each month), for a period of at least one year. This method of adir-inietration is useful and convenient for treating osteoporosis and for rnimmizing adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful for improving patient acceptance and compliance.

Prevention, of osteoporosis.

Alendronate tablets or liquid formulations containing about 70 mg of alendronate, on an alendronic acid active basis, are prepared (see EXAMPLES 7 and 8). The tablets or liquid formulations are orally ad'ministered to a human patient biweekly, i.e. preferably once every fourteen to sixteen days (for example, on about the first and fifteenth of each month), for a period of at least one year. This method of administration is useful and convenient for preventing osteoporosis and for iiiiriimizing adverse gastrointestinal effects, particularly adverse esophageal effects. This method is also useful fop improving patient acceptance and compliance.

EXAMPLE 6 - In further embodiments, alendronate tablets or liquid formulations are orally dosed, at the desired dosage, according to the dosing schedules of EXAMPLES 2-5, for treating or preventing other disorders associated with abnormal bone resorption.

In yet further embodiments, other, bisphosphonate compounds are orally dosed, at the desired dosage, according to the dosing schedules of EXAMPLES 2-5, for treating or preventing osteoporosis or for treating or preventing other conditions associated with abnormal bone resorption.

EXAMPLE 7 Bisphosphonate tablets.

Bispbosphonate containing tablets are prepared using standard mixing and formation techniques as described in U.S. Patent No. 5,358,941, to Bechard et al., issued October 25, 1994, which is incorporated by reference herein in its entirety.

Tablets containing about 35 mg of alendronate, on an . alendronic acid active basis, are prepared using the following relative weights of ingredients.

Ingredient Per Tablet Per 4000 Tablets Alendronate Mono sodium 45.68 mg 182.72 g Trihydrate Anhydrous Lactose, N 71.32 mg 285.28 g Macrocrystalline Cellulose, 80.0 mg 320.0 g NP Magnesium Stearate, NF 1.0 mg 4.0 g Croscarmellose Sodium, NF 2.0 mg 8.0 g The resulting tablets are useful for administration in accordance with the methods of the present invention for inhibiting bone resorption.

Similarly, tablets comprising other relative weights of alendronate, on an alendronic acid active basis are prepared: e.g., about 8.75, 17.5, 70, and 140 mg per tablet. Also, tablets containing other bisphosphonates at appropriate active levels are similarly prepared: e.g., cimadronate, clodronate, tiludronate, etidronate, ibandronate, risedronate, piridronate, pamidronate, zolendronate, and pharmaceutically acceptable salts thereof. Also, tablets containing combinations of bisphosphonates are similarly prepared.

EXAMPLE 8 Liquid Bisphosphonate Formulation.

Liquid bisphosphonate formulations are prepared using standard mixing techniques.

A liquid formulation containing about 70 mg of alendronate monosodium trihydrate, on an alendronic acid active basis, per about 75 mL of liquid is prepared using the following relative weights of ' ingredients.

Ingredient Weight Alendronate Monosodium 91.35 mg Trihydrate Sodium Propylparaben 22.5 mg Sodium Butylparaben 7.5 mg Sodium Citrate Dihydrate 1500 mg Citric Acid Anhydrous 56.25 mg Sodium Saccharin 7.5 mg Water qs 75 mL 1 N Sodium Hydroxide (aq) qs.pH 6.75 The resulting liquid formulation is useful for administration as a unit dosage in .accordance with the methods of the present invention for inhibiting bone resorption.

Similarly, liquid formulations comprising other relative weights of alendronate, on an alendronic acid active basis, per unit dosage are prepared: e.g., about 8.75, 17.5, 35, and 140 mg per 75 mL volume. Also, the liquid formulations are prepared to provide other volumes for the unit dosage, e.g. about .135 mL. Also, the liquid formulations are prepared containing other bisphosphonates at appropriate active levels: e.g., cimadronate, clodronata, tiludronate, etidronate, ibandronate, risedronata, piridronats, pamidronata, zolendronate, and pharmaceutically acceptable salts thereof. Also, liquid formulations containing combinations of bisphosphonates are similarly prepared.

Claims (17)

153109/2 WHAT IS CLAIMED IS:

1. A pharmaceutical composition useful for inhibiting bone resorption in a human comprising from about 70 mg to about 140 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral administration as a unit dosage form according to a continuous schedule having a periodicity selected from once-weekly dosing and bi-weekly dosing.

2. The pharmaceutical composition according to Claim 1, wherein said composition comprises a sodium, potassium, calcium, magnesium or ammonium salt of alendronic acid.

3. The pharmaceutical composition according to Claim 1 , wherein said composition comprises a sodium salt of alendronic acid.

4. The pharmaceutical composition according to Claim 3, wherein said composition comprises the monosodium salt of alendronic acid.

5. The pharmaceutical composition according to Claim 4, wherein the monosodium salt of alendronic acid is hydrated.

6. The pharmaceutical composition according to Claim 1 , wherein said composition comprises the monosodium trihydrate salt of alendronic acid.

7. The pharmaceutical composition according to any one of Claims 1 to 6, useful for treating osteoporosis in a human in need thereof.

8. The pharmaceutical composition according to any one of Claims 1 to 6, useful for preventing osteoporosis in a human in need thereof.

9. The pharmaceutical composition according to any one of Claims 1 to 8, wherein said composition is in the form of a tablet. -36- 153109/2

10. The pharmaceutical composition according to any one of Claims 1 to 8, wherein said composition is in the form of a capsule.

11. 1 1. The pharmaceutical composition according to any one of Claims 1 to 8, wherein said composition is in the form of a liquid.

12. The pharmaceutical composition according to any one of Claims 1 to 1 1 , comprising about 70 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier.

13. The pharmaceutical composition according to any one of Claims 1 to 12, wherein said composition is adapted for oral administration as a unit dosage form, according to a continuous schedule having a periodicity of once-weekly dosing.

14. The pharmaceutical composition according to any one of Claims 1 to 12, wherein said composition is adapted for oral administration as a unit dosage form, according to a continuous schedule having a periodicity of bi-weekly dosing.

15. A pharmaceutical composition useful for inhibiting bone resorption in a human comprising from about 70 mg to about 140 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral administration as a unit dosage form, according to a continuous schedule having a periodicity selected from once-weekly dosing and bi-weekly dosing, essentially as described in the specification.

16. A pharmaceutical composition useful for treating or preventing osteoporosis in a human comprising about 70 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral -37- 153109/1 administration as a unit dosage form, according to a continuous schedule having a periodicity of once-weekly dosing.

17. A pharmaceutical composition useful for treating or preventing osteoporosis in a human comprising about 70 mg of alendronic acid or a pharmaceutically acceptable salt thereof, or a mixture thereof, on an alendronic acid active weight basis, in association with a pharmaceutically acceptable carrier, wherein said composition is adapted for oral administration as a unit dosage form, according to a continuous schedule having a periodicity of bi-weekly dosing. -38-