IL116559A - Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence - Google Patents

Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence

Info

Publication number
IL116559A
IL116559A IL11655995A IL11655995A IL116559A IL 116559 A IL116559 A IL 116559A IL 11655995 A IL11655995 A IL 11655995A IL 11655995 A IL11655995 A IL 11655995A IL 116559 A IL116559 A IL 116559A
Authority
IL
Israel
Prior art keywords
myelin basic
basic protein
mbp
moiety
amino acids
Prior art date
Application number
IL11655995A
Other versions
IL116559A0 (en
Original Assignee
Yissum Res Dev Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL11604495A external-priority patent/IL116044A0/en
Application filed by Yissum Res Dev Co filed Critical Yissum Res Dev Co
Priority to IL11655995A priority Critical patent/IL116559A/en
Publication of IL116559A0 publication Critical patent/IL116559A0/en
Priority to EP96937486A priority patent/EP0862637B1/en
Priority to CNB961994703A priority patent/CN1196791C/en
Priority to DE69634263T priority patent/DE69634263T2/en
Priority to AT96937486T priority patent/ATE287963T1/en
Priority to US09/077,028 priority patent/US6531133B1/en
Priority to JP51956297A priority patent/JP3906447B2/en
Priority to PCT/IL1996/000151 priority patent/WO1997019179A1/en
Priority to KR1019980703699A priority patent/KR100530817B1/en
Priority to AU75040/96A priority patent/AU717617B2/en
Publication of IL116559A publication Critical patent/IL116559A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4713—Autoimmune diseases, e.g. Insulin-dependent diabetes mellitus, multiple sclerosis, rheumathoid arthritis, systemic lupus erythematosus; Autoantigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S424/00—Drug, bio-affecting and body treating compositions
    • Y10S424/81—Drug, bio-affecting and body treating compositions involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, immunotolerance, or anergy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/868—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance

Abstract

A chimeric protein comprising a Pseudomonas aeruginosa exotoxin (PE) moiety linked to a myelin basic protein (MBP) moiety is disclosed. The MBP moiety is selected from the group comprising: (a) MBP; (b) amino acids 69-88 of guinea-pig myelin basic protein or an antigenic portion thereof; (c) amino acids 84-102 of human myelin basic protein or an antigenic portion thereof; (d) amino acids 143-168 of human myelin basic protein or an antigenic portion thereof; and (e) an amino acid sequence in which one or more amino acids have been deleted, added, substituted or mutated in the amino acid sequences of (a), (b), (c) or (d), the modified sequence of (e) retaining at least 75% homology with the amino acid sequences of (a), (b), (c) or (d), respectively. Each of the MBP moieties of (b), (c) and (d) are linked to the PE moiety by a pentapeptide linker repeated 1-3 times. The chimeric protein is useful in treating autoimmune diseases, and especially multiple sclerosis.
IL11655995A 1995-11-17 1995-12-26 Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence IL116559A (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
IL11655995A IL116559A (en) 1995-11-17 1995-12-26 Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence
AU75040/96A AU717617B2 (en) 1995-11-17 1996-11-17 Pseudomonas exotoxin - myelin basic protein chimeric proteins
KR1019980703699A KR100530817B1 (en) 1995-11-17 1996-11-17 Pseudomonas Exotoxin-Myelin Basic Protein Chimeric Proteins
DE69634263T DE69634263T2 (en) 1995-11-17 1996-11-17 Pseudomonas Exotoxin - Myelin Basic Protein Chimeric Proteins
CNB961994703A CN1196791C (en) 1995-11-17 1996-11-17 Psudomonas exotoxin-myelin basic protein chimeric proteins
EP96937486A EP0862637B1 (en) 1995-11-17 1996-11-17 Pseudomonas exotoxin - myelin basic protein chimeric proteins
AT96937486T ATE287963T1 (en) 1995-11-17 1996-11-17 PSEUDOMONAS EXOTOXIN - MYELIN BASIC PROTEIN CHIMERIC PROTEINS
US09/077,028 US6531133B1 (en) 1995-11-17 1996-11-17 Pseudomonas exotoxin-myelin basic protein chimeric proteins
JP51956297A JP3906447B2 (en) 1995-11-17 1996-11-17 Pseudomonas exotoxin-myelin basic protein chimeric protein
PCT/IL1996/000151 WO1997019179A1 (en) 1995-11-17 1996-11-17 Pseudomonas exotoxin - myelin basic protein chimeric proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL11604495A IL116044A0 (en) 1995-11-17 1995-11-17 Chimeric proteins
IL11655995A IL116559A (en) 1995-11-17 1995-12-26 Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence

Publications (2)

Publication Number Publication Date
IL116559A0 IL116559A0 (en) 1996-03-31
IL116559A true IL116559A (en) 2005-11-20

Family

ID=26323172

Family Applications (1)

Application Number Title Priority Date Filing Date
IL11655995A IL116559A (en) 1995-11-17 1995-12-26 Chimeric protein consisting of a bacterial toxin and a myelin basic protein sequence

Country Status (10)

Country Link
US (1) US6531133B1 (en)
EP (1) EP0862637B1 (en)
JP (1) JP3906447B2 (en)
KR (1) KR100530817B1 (en)
CN (1) CN1196791C (en)
AT (1) ATE287963T1 (en)
AU (1) AU717617B2 (en)
DE (1) DE69634263T2 (en)
IL (1) IL116559A (en)
WO (1) WO1997019179A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6750329B1 (en) * 1989-05-05 2004-06-15 Research Development Foundation Antibody delivery system for biological response modifiers
US6252040B1 (en) * 1991-10-22 2001-06-26 The Governors Of The University Of Alberta Peptide specificity of anti-myelin basic protein and the administration of myelin basic protein peptides to multiple sclerosis patients
IL116436A (en) 1995-12-18 2006-12-31 Yissum Res Dev Co Fc?Á-PE CHIMERIC PROTEIN FOR TARGETED TREATMENT OF ALLERGY RESPONSES AND
US20030054012A1 (en) * 2000-05-12 2003-03-20 Fitzgerald David J. Pseudomonas exotoxin a-like chimeric immunogens for eliciting a secretory iga-mediated immune response
ATE307208T1 (en) * 1997-07-11 2005-11-15 Us Gov Health & Human Serv PSEUDOMONAS EXOTOXIN-A-LIKE CHIMERIC IMMUNOGENS
US7314632B1 (en) 1997-07-11 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pseudomonas exotoxin A-like chimeric immunogens
US6645490B2 (en) 1998-03-02 2003-11-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Chimeric proteins with cell-targeting specificity and apoptosis-inducing activities
ZA200305980B (en) 2001-02-12 2007-01-31 Res Dev Foundation Modified proteins, designer toxins, and methods of making thereof
JP2004535202A (en) * 2001-07-17 2004-11-25 リサーチ　ディベロップメント　ファンデーション Therapeutic agents containing pro-apoptotic proteins
WO2003105761A2 (en) * 2002-06-12 2003-12-24 Research Development Foundation Immunotoxin as a therapeutic agent and uses thereof
AU2004234191A1 (en) 2003-04-30 2004-11-11 University Of Zurich Methods for treating cancer using an immunotoxin
EP1855724A2 (en) * 2005-02-01 2007-11-21 Research Development Foundation Blys fusion proteins for targeting blys receptor and methods for treatment of b-cell proliferative disorders
WO2011101870A1 (en) * 2010-02-22 2011-08-25 Transgene Biotek Ltd. Fusion proteins for the treatment of multiple sclerosis and other autoimmune diseases
CN102167732B (en) * 2010-12-10 2013-08-21 厦门大学 Protein type algicidal compound and preparation method thereof
EP4029937A1 (en) 2012-10-04 2022-07-20 Research Development Foundation Serine protease molecules and therapies
US11193117B2 (en) 2016-02-16 2021-12-07 Research Development Foundation Cell-targeted cytotoxic constructs
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL36670A (en) 1971-04-21 1974-09-10 Sela M Therapeutic basic copolymers of amino acids
FR2521010B1 (en) * 1982-02-09 1985-07-19 Sanofi Sa MEDICINAL PRODUCTS COMPRISING AT LEAST ONE IMMUNOTOXIN AND AT LEAST ONE MONOVALENT CARBOXYLIC IONOPHORE
US5849298A (en) * 1987-06-24 1998-12-15 Autoimmune Inc. Treatment of multiple sclerosis by oral administration of bovine myelin
US5571499A (en) * 1987-06-24 1996-11-05 Autoimmune, Inc. Treatment of autoimmune diseases by aerosol administration of autoantigens
US5869054A (en) * 1987-06-24 1999-02-09 Autoimmune Inc. Treatment of multiple sclerosis by oral administration of autoantigens
US5571500A (en) * 1987-06-24 1996-11-05 Autoimmune, Inc. Treatment of autoimmune diseases through administration by inhalation of autoantigens
JP2849632B2 (en) * 1988-04-08 1999-01-20 社団法人北里研究所 Vaccine preparation
US5468481A (en) * 1988-06-23 1995-11-21 Amergen, Inc. MHC class II-peptide conjugates useful in ameliorating autoimmunity
DE69027210T2 (en) 1989-04-21 1997-01-23 Us Health RECOMBINANT ANTIBODY TOXIN FUSION PROTEIN
WO1991018018A1 (en) 1990-05-14 1991-11-28 The United States Of America, Represented By The Secretary, United States Department Of Commerce Improved pseudomonas chimeric protein cytotoxic to human cells bearing il2 receptors
US5734023A (en) * 1991-11-19 1998-03-31 Anergen Inc. MHC class II β chain/peptide complexes useful in ameliorating deleterious immune responses
DK0863155T3 (en) 1992-04-09 2002-05-13 Autoimmune Inc Suppression of T cell proliferation using peptide fragments of myelin basic protein
US6045796A (en) * 1992-12-17 2000-04-04 Anergen, Inc. Vaccination with peptide of MHC class II molecules for treatment of autoimmune disease
US5681571A (en) * 1993-10-08 1997-10-28 Duotol Ab Immunological tolerance-inducing agent
US5763585A (en) * 1993-10-13 1998-06-09 Anergen, Inc. Method of making MHC-peptide complexes using metal chelate affinity chromatography
US5824315A (en) * 1993-10-25 1998-10-20 Anergen, Inc. Binding affinity of antigenic peptides for MHC molecules
US5759546A (en) * 1994-02-04 1998-06-02 Weinberg; Andrew D. Treatment of CD4 T-cell mediated conditions
US5935577A (en) * 1998-01-23 1999-08-10 Autoimmune Inc. Treatment of autoimmune disease using tolerization in combination with methotrexate

Also Published As

Publication number Publication date
DE69634263T2 (en) 2005-12-22
CN1207132A (en) 1999-02-03
DE69634263D1 (en) 2005-03-03
IL116559A0 (en) 1996-03-31
US6531133B1 (en) 2003-03-11
AU717617B2 (en) 2000-03-30
EP0862637B1 (en) 2005-01-26
JP3906447B2 (en) 2007-04-18
JP2000513562A (en) 2000-10-17
KR100530817B1 (en) 2006-01-27
ATE287963T1 (en) 2005-02-15
EP0862637A1 (en) 1998-09-09
WO1997019179A1 (en) 1997-05-29
KR19990067671A (en) 1999-08-25
CN1196791C (en) 2005-04-13
AU7504096A (en) 1997-06-11

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Legal Events

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FF Patent granted
KB Patent renewed
MM9K Patent not in force due to non-payment of renewal fees