IES20001033A2 - An improved process for producing crystalline atorvastation calcium - Google Patents

Abstract

A factory scale process for producing crystalline atorvastatin trihydrate hemi calcium salt includes the addition of extra methyl tert-butyl ether to the reaction mixture to supersaturate the crystallisation matrix. A seed slurry is made up in a make-up/delivery vessel and delivered, under pressure, to the reaction mixture. The process produces crystalline atorvastatin calcium within a consistent size range on a factory scale.

Description

The invention relates to an improved process for producing crystalline atorvastatin calcium which is known by the chemical name [R-(R*,R*)]-2-(4-fluorophenyl)P,0-dihydroxy-5-(l-methylethyl)-3-phenyl-4~[(phenylamino)carbonyl]-lHpyrrole-1-heptanoic acid hemi calcium salt.

Atorvastatin is useful as a selective and competitive inhibitor of the enzyme 3hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, the ratelimiting enzyme that converts 3-hydroxy-3-methylglutaryl-coenzyme A to mevalonate, a precursor of sterols such as cholesterol. The conversion of HMGCoA to mevalonate is an early and rate-limiting step in cholesterol biosynthesis.

Atorvastatin as well as some of its metabolites are pharmacologically active in humans and are thus useful as a hypolipidemic and hypocholesterolemic agent. The liver is the primary site of action and the principal site of cholesterol synthesis. Clinical and pathological studies show that elevated plasma levels of total cholesterol and associated triglycerides promote human atherosclerosis and are risk factors for developing cardiovascular disease.

United States Patent Number 4,681,893, which is herein incorporated by reference, discloses certain trans-6-[2-(3- or 4-carboxamido-substituted-pyrrol1- yl) alkyl]—4 -hydroxy-pyran-2-ones including trans (±)-5-(4-fluorophenyl)-2(l-methylethyl)-N,4-diphenyl-l-[(2-tetrahydro-4-hydroxy-6-oxo-2H-pyran2— yl)ethy 1]—1 H-pyrrole-3-carboxamide.

United States Patent Number 5,273,995, which is herein incorporated by reference, discloses the enantiomer having the R form of the ring-opened acid of trans -5-(4-fluorophenyl)-2-(l-methylethyl)-N, 4-diphenyl-l-[(2-tetrahydro-430 -2WARN03/C/IESD hydroxy-6-oxo-2H-pyran-2-yl)ethyl]-1 H-pyrr ole-3-carboxamide, i. e., [R(R*,R*)]-2-(4-fluorophenyl)-p, 0-dihydroxy-5-(l-methylethyl)-3-phenyl-4[(phenylamino) carbonyl]-lH-pyrrole-l-heptanoic acid.

The above described atorvastatin compounds have been prepared by a superior convergent route disclosed in the following United States Patent Numbers 5,003,080; 5,097,045; 5,103,024; 5,124,482 and 5,149,837 which are herein incorporated by reference and Baumann K.L., Butler D.E., Deering C.F., et al, Tetrahedron Letters 1992;33:2283-2284.

One of the critical intermediates disclosed in United States Patent Number 5,097,045 has also been produced using novel chemistry, as disclosed in United States Patent Number 5,155,251, which is herein incorporated by reference and Brower P.L., Butler D.E., Deering C.F., et al, Tetrahedron Letters 1992;33:22792282.

United States Patent Numbers 5,216,174; 5,245,047; 5,248,793; 5,280,126; 5,397,792; 5,342,952; 5,298,627; 5,446,054; 5,470,981; 5,489,690; 5,489,691; 5,5109,488; W097/03960; WO98/09543 and WO99/32434 which are herein incorporated by reference, disclose various processes and key intermediates for preparing atorvastatin.

It has been found that when the process for preparing atorvastatin calcium was scaled up to a commercial factory scale drying was slow and difficult to optimise.

It was also found that wet crystalline atorvastatin calcium was susceptible to possible break up with physical attrition and furthermore had a propensity to form rock hard clods on mixing.

The object of the present invention is therefore to provide a process for producing crystalline atorvastatin calcium on a factory scale which routinely and consistently produces high quality material with reduced cycle time. -3WARN03/C/IESD Statements of Invention According to the invention there is provided a process for producing crystalline atorvastatin calcium comprising the steps of reacting a mixture of atorvastatin lactone and methyl tert-butyl ether with sodium hydroxide to form the ring-opened sodium salt; extracting the aqueous layer thus formed with methyl tert-butyl ether; adding a charge of methyl tert-butyl ether to a vessel containing the product rich aqueous layer to supersaturate the crystallisation matrix; sealing the reaction vessel; heating the contents of the vessel to 47 to 57°C; adding calcium acetate hemihydrate to form atorvastatin trihydrate hemi calcium salt; and drying the isolated product in a vacuum pan dryer having an agitator which is continuously rotated at a speed of from 0.5 to 2rpm.

In a particularly preferred embodiment of the invention the agitator is substantially continuously rotated at a speed of approximately lrpm.

In a preferred embodiment the vacuum in the pan dryer is maintained at from -0.80 to -0.99 bar.

IE ο Ο 1 Ο 3 3 WARNO3/C/IESD -4Preferably the isolated product is dried over a period of from 1 to 4 days, ideally over a period of from 1 to 2 days.

Detailed description Atorvastatin lactone is saponified in a water/methyl alcohol/methyl tert-butyl ether (2-methoxy-2-methyl-propane; tert-butyl methyl ether) mixture with sodium hydroxide. The aqueous layer containing the sodium salt of atorvastatin is washed with methyl tert-butyl ether to remove small quantities of process impurities. A small aliquot of methyl tert-butyl ether is added to the crystallisation matrix. Sodium-to-calcium salt metathesis with concurrent crystallisation is accomplished by the slow addition of an aqueous calcium acetate solution to the sodium salt solution. To assure crystallisation simultaneous with addition, the reaction mixture is seeded with crystalline atorvastatin shortly after the start of the calcium acetate addition. The product is isolated by filtration and, after washing with water/methyl alcohol and water is centrifuged and vacuum dried before milling to give crystalline atorvastatin as the trihydrate. The reaction scheme is shown below.

F ΌΗ 2. Ca(OAc) F 1. 2NaOH(aq)/ MTBE/MeOH O' Ca++ ,3H2O + 2NaOAc Scheme 1 WARN03/C/IESD -5The drying of atorvastatin calcium has a sensitive window of moisture content where the drying has to proceed slowly in order to prevent the particles from breaking down.

We have found that continuous agitation at approximately lrpm significantly reduces the drying time compared to an intermittent agitation technique, thereby increasing drying capacity while ensuring that the final dried product is within a consistent range for particle size and bulk density. This is in complete contrast to continuous high speed agitation which results in clod formation and the risk of physical attrition and intermittent agitation which substantially increases the required drying time.

The present invention describes an improved drying system using an agitated pan dryer such as a jacketed Guedu Pan Dryer. The agitator is rotated continuously at a speed of approximately 1 rpm to provide reduced drying time while ensuring uniform drying of the crystals and avoidance of clod formation.

It has been surprisingly found that this continuous agitation at a very low speed provides optimum drying conditions with very significant increased throughput while avoiding clod formation and particle attrition.

Example 1 250kg atorvastatin lactone prepared as described in US 5,273,995 the entire contents of which are incorporated by reference, 1028kg methyl tert- butyl ether and 496kg of methanol are charged to a 6000 litre glass lined reaction vessel. The reaction mixture is agitated and heated to about 30°C to dissolve the lactone. When the lactone is dissolved, approximately 3200 litres of caustic solution is added (19kg of sodium hydroxide 97.5% dissolved in 3165 litres of deionised water). The contents of the vessel are heated to 47 to 57°C and agitated for at least 45 minutes. -6-/ & WARN03/C/IESD After cooling to 25 to 35°C under an inert atmosphere the contents are allowed to settle and the organic layer is discarded. 765kg methyl tek-butyl ether is charged to the aqueous layer, the contents mixed and allowed to settle. The organic layer is discarded. 63kg of extra methyl tert-butyl ether is charged to the product rich aqueous layer in the reaction vessel which is then sealed. The contents of the sealed reaction vessel are heated to 47 to 57°C maintaining a pressurised system.

A solution of calcium acetate (40kg calcium acetate hemihydrate in 1365 litres deionised water) is transferred to the pressurised vessel. Shortly after commencement of the calcium acetate addition the transfer is stopped and atorvastatin trihydrate hemi calcium salt Form I seed, prepared as described in US5,969,156 which is herein incorporated by reference, is introduced.

A seed slurry is prepared by charging 37 litres deionised water and 13kg methanol to a stainless steel make-up/delivery vessel. The solvent mixture is agitated by rocking the vessel back and forth on its cradle. 3.6kg atorvastatin calcium seed crystals are then charged to the solvent mixture. The contents of the delivery vessel are mixed by rocking until a seed slurry is formed. Sufficient pressure is applied to the make-up/delivery delivery vessel so that its pressure is higher than that of the reaction vessel. The make-up/delivery vessel is attached to the reaction vessel via a flexible hose and the seed slurry is charged rapidly over 2 to 3 minutes, under pressure, into the reaction vessel.

After the addition of the seed slurry the calcium acetate addition is immediately resumed to complete the calcium transfer.

The product cake is washed first with a methanol/water solution followed by a water wash.

WARN03/C/IESD Ί - Example 2: Drying of atorvastatin calcium The wet product prepared in Example 1 is loaded into a stainless steel continuous 5 agitation pan dryer such as a Guedu Pan Dryer. A full vacuum of from -0.80 to 0.99 bar is applied, the jacket of the dryer adjusted to 60 to 70°C and the product dried with slow continuous agitation at approximately lrpm for at least 24 hours, preferably for from 1 to 4 days, ideally 1 to 2 days.

The dry product is then loaded into clean poly-lined drums.

The invention is not limited to the embodiments hereinbefore described which may be varied in detail.

Claims (5)

Claims

1. A factory scale process for producing crystalline atorvastatin calcium comprising the steps of reacting a mixture of atorvastatin lactone and methyl tert-butyl ether with sodium hydroxide to form the ring-opened sodium salt; extracting the aqueous layer thus formed with methyl tert-butyl ether; adding a charge of methyl tert-butyl ether to a vessel containing the product rich aqueous layer to supersaturate the crystallisation matrix; sealing the reaction vessel; heating the contents of the vessel to 47 to 57°C; adding calcium acetate hemihydrate to form atorvastatin trihydrate hemi calcium salt; and drying the isolated product in a vacuum pan dryer having an agitator which is continuously rotated at a speed of from 0.5 to 2rpm.

2. A process as claimed in claim 1 wherein the agitator is substantially continuously rotated at a speed of approximately lrpm.

3. A process as claimed in claim 1 or 2 wherein the vacuum in the pan dryer is maintained at from -0.80 to -0.99 bar. WARN03/C/IESD -94. A process as claimed in any preceding claim wherein the isolated product is dried over a period of from 1 to

4. Days, preferably the isolated product is dried over a period of from 1 to 2 days.

5. 5. Crystalline atorvastatin calcium prepared by a process as claimed in any preceding claim.