IE904517A1 - Process for the preparation of 2-acylamino-9-acyl-6-halopurine - Google Patents
Process for the preparation of 2-acylamino-9-acyl-6-halopurineInfo
- Publication number
- IE904517A1 IE904517A1 IE451790A IE451790A IE904517A1 IE 904517 A1 IE904517 A1 IE 904517A1 IE 451790 A IE451790 A IE 451790A IE 451790 A IE451790 A IE 451790A IE 904517 A1 IE904517 A1 IE 904517A1
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- IE
- Ireland
- Prior art keywords
- compound
- formula
- acid
- molar ratio
- preparation
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D473/00—Heterocyclic compounds containing purine ring systems
- C07D473/40—Heterocyclic compounds containing purine ring systems with halogen atoms or perhalogeno-alkyl radicals directly attached in position 2 or 6
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
Abstract
2-Acylamino-9-acyl-6-halogeno-purine can be prepared by acylating 2-acylamino-6-halogeno-purine in the presence of a catalyst without the contaminating production of 2-diacylamino-9-acyl-6-chloro-purine.
Description
Description Process for the preparation of 2-acylamino-9-acyl-6halopurine 2-Acylamino-9-acyl-6-chloropurines are very widely utilizable intermediates in the pharmaceutical industry for nucleoside syntheses.
The preparation of 2-acetamido-9-acetyl-6-chloropurine by acetylation of 2-acetamido-6-chloropurine has already been described (Synthetic Procedures in Nucleic Acid Chemistry, 1, 1968, W.W. Zorbach and R.S. Tipson, 25 27). In this case, the acetylation in position 9 of the purine ring is preferably carried out under reflux conditions. However, even after 5 to 10 minutes reaction time, 2-diacetamido-9-acetyl-6-chloropurine is also formed as an impurity in addition to 2-acetamido-6-chloropurine. The total yield of the two compounds is 75%.
Some experiments moreover show that under the abovementioned conditions at reflux temperatures of 135°C to 140°C, not only are larger amounts of 2-diacetamido-9acetyl-6-chloropurine formed, but a replacement of chlorine by oxygen in position 6 of the purine ring additionally also takes place. These disadvantages particularly have an influence in the preparation of larger amounts of 2-acetamido-9-acetyl-6-chloropurine. On the production scale, longer heating and cooling times cannot be avoided, so that the high thermal loadings lead to strongly contaminated 2-acetamido-9-acetyl-6-chloropurine batches.
Surprisingly, it has now been found that by the use of a catalyst the purity of 2-acylamino-9-acyl-6-halopurine can be substantially improved in the preparation from 2acylamino-6-halopurine.
The invention thus relates to a process for the preparation of the compound of the formula I in which R1 and R2, which can be the same or different, are a straight-chain or branched acyl group having 2 to carbon atoms or an aroyl group having 7 to 10 carbon atoms and X is fluorine, chlorine, bromine or iodine, by acylation of the compound of the formula II in which R1 and X have the abovementioned meaning, wherein the acylation takes place in the presence of a catalyst.
The invention will now be described in detail in the following, in particular in its preferred embodiments.
The expression acyl group having 2 to 10 carbon atoms is to be understood as meaning, for example, the radicals of the following acids: acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, i-valeric acid, methylethylacetic acid, trimethylacetic acid, caproic acid, caprylic acid, capric acid, heptanoic acid and nonanoic acid. The expression aroyl group having 7 to 10 carbon atoms means, - 3 for example, the radicals of the following compounds: benzoic acid, phenylacetic acid, phenylpropanoic acid, phenyl-n-butyric acid or phenyl-i-butyric acid.
The reaction, according to the invention, of 2-acylamino5 6-halopurine to give 2-acylamino-9-acyl-6-halopurine is carried out in the presence of an acylating agent, with the aid of which the amino group in the 9-position of the compound of the formula II can be substituted by a straight-chain or branched acyl group having 2 to 10 carbon atoms or an aroyl group having 7 to 10 carbon atoms. Acylating agents which can be used are carboxylic anhydrides, such as, for example, acetic anhydride, propionic anhydride, butyric anhydride, valeric anhydride, caprylic anhydride, capric anhydride or caproic anhydride. However, mixed anhydrides can also be employed. In addition, acylating agents which can also be used are carboxylic acid halides, such as acetyl chloride, propionyl chloride, phenylacetyl chloride, phenylbutanoyl chloride, benzoyl chloride, acetyl bromide, or propionyl bromide. The acylating agents can be dissolved in an inert solvent beforehand. Inert solvents which can be used are compounds such as, for example, dimethylacetamide, toluene, benzene or N-methylpyrrolidone. 2-Acylamino-6-halopurine is mixed with the acylating agent and heated with the addition of a catalyst. The following compounds can be used as catalyst: pyridine, pyridine derivatives such as, for example, 4dimethylaminopyridine, 4-(4-methyl-l-piperidinyl)30 pyridine, 4-pyrrolidinopyridine, 4-morpholinopyridine or N-methylimidazole, and also mineral acids such as, for example, hydrochloric acid or sulphuric acid. Preferably, 4-dimethylaminopyridine is used.
The molar ratio of 2-acylamino-6-halopurine to the acylating agent can be varied within a range from 1:1 to 1:40. The reaction is preferably carried out in a range - 4 from 1:5 to 1:30. The molar ratio of catalyst to 2acylamino-6-halopurine can vary within a range from 1:5 to 1:100. Preferably, a molar ratio of 1:35 to 1:80 is maintained.
The reaction temperature is between 10 *C and 9O’C, in particular between 20’C and 70’C. The reaction time is between 0.5 and 7 hours, in particular between 1 and 3 hours. The course of the reaction is determined by sample removal and quantitative analysis of the final product with the aid of known titration methods. After this, the mixture is stirred at temperatures between O’C and 10’C for a further 0.5 to 2 hours. After filtration, the compound of the formula I is obtained in high purity.
The sequence of the individual process steps can also be varied. A person skilled in the art does not find it difficult to find the optimum sequence for the reaction process.
The preparation of 2-acylamino-6-halopurines is carried out in a manner known per se by halogenation and acylation of purines (EP 0,203,685; D.J. Brown, Heterocyclic Compounds, 135-197, 1971; Bowles W.A. et al., J. Med. Chem., 71-74, 6, 1963).
In comparison to the route known hitherto for the acylation of 2-acylamino-6-halopurines, the process according to the invention opens up the advantage of obtaining the final product in high purity and yield. The catalysts used catalyze the acylation in position 9 of the purine ring, but not the formation of 2-diacylamino9-acyl-6-halopurines. All process steps which are needed for the separation of the 2-diacylamino-9-acyl-6-halopurine derivatives thus become unnecessary.
The example shown below is used to illustrate the invention further.
Example: 1.1 kg (5.2 mol) of 2-acetamido-6-chloropurine are suspended in 11 1 of acetic anhydride and 87.0 g (0.71 mol) of 4-dimethylaminopyridine are added. The mixture is stirred at 50°C for two hours, then cooled and stirred at O’C to 5’C for half an hour. The product is filtered off, washed with diisopropyl ether and dried in vacuo at 50°C. 1.16 kg of 2-acetamido-9-acetyl-6-chloropurine are obtained.
Yield: 88% of theory Purity: 99.1% (titration) II 1H-NMR (in DMSO): 6 (ppm): 2.25 2.93 n-c-ch3 9-COCH3 15 8.93 8-H 11.06 HN-CO-C HOE 89/F 395
Claims (12)
1. A process for the preparation of the compound of the formula I Rin which R 1 and R 2 , which can be the same or different, are a straight-chain or branched acyl group having 2 to 10 carbon atoms or an aroyl group having 7 to 10 carbon atoms and X is fluorine, chlorine, bromine or iodine, by acylation of the compound of the formula II HN X I II in which R 1 and X have the abovementioned meaning, wherein the acylation takes place in the presence of a catalyst.
2. The process as claimed in claim 1, wherein carboxylic anhydride or carboxylic acid halide is used as the acylating agent.
3. The process as claimed in one or more of claims 1 and 2, wherein pyridine, pyridine derivatives or mineral acids are used as the catalyst. 4. The process as claimed in claim 3, wherein
4.-dimethylaminopyridine 4-(4-methyl-l-piperidinyl)-pyridine, 4-pyrrolidinopyridine, 4-morpholinopyridine or Nmethylimidazole is used as the pyridine derivative and hydrochloric acid or sulphuric acid is used as the mineral acid.
5. The process as claimed in one or more of claims 1 to 4, wherein the molar ratio of the compound of the formula II to acylating agent is in the range from 1:1 to 1:40.
6. The process as claimed in claim 5, wherein the molar ratio is in the range from 1:5 to 1:30.
7. The process as claimed in one or more of claims 1 to 6, wherein the molar ratio of catalyst to the compound of the formula II is in the range from 1:5 to 1:100.
8. The process as claimed in claim 7, wherein the molar ratio is between 1:35 and 1:80.
9. The process as claimed in one or more of claims 1 to 8, wherein the reaction temperature is between 10’C and 90°C.
10. The process as claimed in claim 9, wherein the reaction temperature is between 20°C and 70°C.
11. A process according to claim 1 for the preparation of a compound of the formula I given and defined therein, substantially as hereinbefore described and exemplified.
12. A compound of the formula I given and defined in claim 1, whenever prepared by a process claimed in a preceding claim.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3941658A DE3941658A1 (en) | 1989-12-16 | 1989-12-16 | METHOD FOR PRODUCING 2-ACYLAMINO-9-ACYL-6-HALOGEN PURIN |
Publications (2)
Publication Number | Publication Date |
---|---|
IE904517A1 true IE904517A1 (en) | 1991-06-19 |
IE67294B1 IE67294B1 (en) | 1996-03-20 |
Family
ID=6395643
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE451790A IE67294B1 (en) | 1989-12-16 | 1990-12-14 | Process for the preparation of 2-acylamino-9-acyl-6-halopurine |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0433845B1 (en) |
JP (1) | JPH0692965A (en) |
AT (1) | ATE120748T1 (en) |
DE (2) | DE3941658A1 (en) |
DK (1) | DK0433845T3 (en) |
ES (1) | ES2071731T3 (en) |
IE (1) | IE67294B1 (en) |
PT (1) | PT96179B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7321035B2 (en) | 2001-04-05 | 2008-01-22 | Sumitomo Chemical Company, Limited | Process for producing 2,6-dihalogenopurine |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9201961D0 (en) * | 1992-01-30 | 1992-03-18 | Smithkline Beecham Plc | Pharmaceuticals |
JPH07133276A (en) * | 1993-09-17 | 1995-05-23 | Jiyuuzen Kagaku Kk | Production of 2-acetylamono-6-chloropurine |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI74468C (en) * | 1982-10-14 | 1988-02-08 | Wellcome Found | FOERFARANDE FOER FRAMSTAELLNING AV TERAPEUTISKT AKTIVA 2-AMINOPURINDERIVAT. |
EP0138683A3 (en) * | 1983-09-30 | 1988-01-20 | Merck & Co. Inc. | Purine derivatives, their application in anti-viral compositions |
-
1989
- 1989-12-16 DE DE3941658A patent/DE3941658A1/en not_active Withdrawn
-
1990
- 1990-12-11 DK DK90123792.5T patent/DK0433845T3/en active
- 1990-12-11 ES ES90123792T patent/ES2071731T3/en not_active Expired - Lifetime
- 1990-12-11 DE DE59008852T patent/DE59008852D1/en not_active Expired - Fee Related
- 1990-12-11 EP EP90123792A patent/EP0433845B1/en not_active Expired - Lifetime
- 1990-12-11 AT AT90123792T patent/ATE120748T1/en not_active IP Right Cessation
- 1990-12-13 PT PT96179A patent/PT96179B/en not_active IP Right Cessation
- 1990-12-14 JP JP2419202A patent/JPH0692965A/en active Pending
- 1990-12-14 IE IE451790A patent/IE67294B1/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7321035B2 (en) | 2001-04-05 | 2008-01-22 | Sumitomo Chemical Company, Limited | Process for producing 2,6-dihalogenopurine |
Also Published As
Publication number | Publication date |
---|---|
DK0433845T3 (en) | 1995-07-31 |
DE3941658A1 (en) | 1991-06-20 |
DE59008852D1 (en) | 1995-05-11 |
ES2071731T3 (en) | 1995-07-01 |
JPH0692965A (en) | 1994-04-05 |
EP0433845A1 (en) | 1991-06-26 |
ATE120748T1 (en) | 1995-04-15 |
PT96179B (en) | 1998-04-30 |
IE67294B1 (en) | 1996-03-20 |
PT96179A (en) | 1991-09-30 |
EP0433845B1 (en) | 1995-04-05 |
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