HUP0202805A2 - Célzott géneltávolítás - Google Patents

Célzott géneltávolítás

Info

Publication number
HUP0202805A2
HUP0202805A2 HU0202805A HUP0202805A HUP0202805A2 HU P0202805 A2 HUP0202805 A2 HU P0202805A2 HU 0202805 A HU0202805 A HU 0202805A HU P0202805 A HUP0202805 A HU P0202805A HU P0202805 A2 HUP0202805 A2 HU P0202805A2
Authority
HU
Hungary
Prior art keywords
plant
targeted gene
gene removal
removal
genes
Prior art date
Application number
HU0202805A
Other languages
English (en)
Inventor
Peter Meyer
Elena Zubko
Original Assignee
University Of Leeds
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Leeds filed Critical University Of Leeds
Publication of HUP0202805A2 publication Critical patent/HUP0202805A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A találmány tárgyát egy, szelektálható marker gén vagy gének vagyidegen kisegítő nukleinsavak növényből, közelebbről transzgénikusnövényből történő eltávolítására szolgáló rekombináns eljárás képezi,továbbá a találmány szerinti eljárás megvalósításához szükségesvektorok és a találmány szerinti eljárás termékei. A találmányszerinti megoldás a növénytermesztésben és növénynemesítésbenalkalmazható. Ó
HU0202805A 1999-09-17 2000-09-15 Célzott géneltávolítás HUP0202805A2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9921937.0A GB9921937D0 (en) 1999-09-17 1999-09-17 Targeted gens removal
PCT/GB2000/003543 WO2001021780A2 (en) 1999-09-17 2000-09-15 Targeted gene removal

Publications (1)

Publication Number Publication Date
HUP0202805A2 true HUP0202805A2 (hu) 2002-12-28

Family

ID=10861053

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0202805A HUP0202805A2 (hu) 1999-09-17 2000-09-15 Célzott géneltávolítás

Country Status (10)

Country Link
EP (1) EP1212443A2 (hu)
JP (1) JP2003510043A (hu)
CN (1) CN1375010A (hu)
AU (1) AU7532500A (hu)
BR (1) BR0014521A (hu)
CA (1) CA2384932A1 (hu)
GB (1) GB9921937D0 (hu)
HU (1) HUP0202805A2 (hu)
RU (1) RU2002108173A (hu)
WO (1) WO2001021780A2 (hu)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
EP1276884A2 (en) * 2000-04-20 2003-01-22 Btg International Limited Transgenic plants
CA2608636C (en) * 2005-05-17 2015-02-10 Frank Koentgen Sequential cloning system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
NL8701450A (nl) * 1987-06-22 1989-01-16 Solvay Werkwijze voor het transformeren van cellen.
WO1991019796A1 (en) * 1990-06-12 1991-12-26 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
US5736367A (en) * 1992-03-31 1998-04-07 Medimmune, Inc. Vectors and prokaryotes which autocatalytically delete antibiotic resistance
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
ATE355384T1 (de) * 1996-05-09 2006-03-15 Jujo Paper Co Ltd Vektor zum gentransfer in pflanzen, der eine wahlweise deletion des markergens erlaubt
CN100342008C (zh) * 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
AU6364800A (en) * 1999-07-23 2001-02-13 Regents Of The University Of California, The Dna recombination in eukaryotic cells by the bacteriophage phic31 recombination system

Also Published As

Publication number Publication date
CN1375010A (zh) 2002-10-16
GB9921937D0 (en) 1999-11-17
EP1212443A2 (en) 2002-06-12
RU2002108173A (ru) 2004-03-10
AU7532500A (en) 2001-04-24
CA2384932A1 (en) 2001-03-29
BR0014521A (pt) 2002-06-11
WO2001021780A2 (en) 2001-03-29
WO2001021780A3 (en) 2001-10-18
JP2003510043A (ja) 2003-03-18

Similar Documents

Publication Publication Date Title
ATE330007T1 (de) Verfahren zur erhöhung der expression von endogenen oder exogenen genprodukten in pflanzen
DE60335637D1 (de) Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren
HUP0002578A2 (hu) Hímszövet-specifikus szabályzó régió és alkalmazására szolgáló eljárás
DE69939744D1 (de) Verfahren zur herstellung stresstoleranter pflanzen
DE69430993D1 (de) Kontrollierte expression transgener konstrukte in pflanzenplastiden
DE69333529D1 (de) Pflanzenvirus-vektor, plasmid, methode der expression fremder gene und methode zur gewinnung obiger genprodukte
MXPA02004884A (es) Genes de tolerancia a patogenos.
DE60236182D1 (de) Zufällige integration von polynukleotide nach in vivo linearisierung
ATE323163T1 (de) Weizen rht gen zur genetischen kontrolle des pflanzenwachstums und entwicklung
DK1228227T3 (da) Hankönssterile Brassica-planter samt fremgangsmåder til deres fremstilling
Kawakami et al. Excision of the Tol2 transposable element of the medaka fish Oryzias latipes in Xenopus laevis and Xenopus tropicalis
WO2004081173A3 (en) Nucleotide sequences regulating gene expression and constructs and methods utilizing same
HUP0202608A2 (hu) Magpreferenciával jellemzett promóter kukoricából
HUP0002649A2 (hu) Hímsterilitás létrehozása növényekben, avidin magas szintű expresszáltatásával
ATE313636T1 (de) Dna kodierend für pflanzliche lipase, transgene pflanzen und ein verfahren zur kontrolle der seneszenz in pflanzen
DE60332107D1 (de) Saatspezifische usp-promotoren zur expression von genen in pflanzen
WO2001070777A3 (en) Leafy cotyledon2 genes and their uses
DE60225312D1 (de) Samenspezifische 7s-alpha-promoter zur expression von genen in pflanzen
HUP0202805A2 (hu) Célzott géneltávolítás
ATE338814T1 (de) Verfahren zur kultivierung von zellen und zur vermehrung von viren
DE60035892D1 (de) Gene und verfahren zur wachstumsmanipulation
DE60024658D1 (de) Promotor zur regulierung von fremdgenexpression
DE60138972D1 (de) Transgene Pflanzen mit dem Gen des Neoxanthin spaltenden Enzyms
HUP0202852A2 (hu) Módosított ubiquitin-szabályozó rendszer
AU6795201A (en) Nucleic acid sequences and methods for the modification of plant gene expression