HK1249133A1 - 一種用於無創產前檢測的文庫構建方法及試劑盒 - Google Patents

一種用於無創產前檢測的文庫構建方法及試劑盒

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Publication number
HK1249133A1
HK1249133A1 HK18108558.7A HK18108558A HK1249133A1 HK 1249133 A1 HK1249133 A1 HK 1249133A1 HK 18108558 A HK18108558 A HK 18108558A HK 1249133 A1 HK1249133 A1 HK 1249133A1
Authority
HK
Hong Kong
Prior art keywords
kit
construction method
invasive prenatal
prenatal detection
detection
Prior art date
Application number
HK18108558.7A
Other languages
English (en)
Inventor
張艷艷
江媛
李巧玲
魏漢敏
趙霞
董超
沈寒婕
田凱
于競
傅書錦
蘇小珊
耿春雨
Original Assignee
深圳華大基因科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 深圳華大基因科技有限公司 filed Critical 深圳華大基因科技有限公司
Publication of HK1249133A1 publication Critical patent/HK1249133A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK18108558.7A 2016-11-21 2018-07-03 一種用於無創產前檢測的文庫構建方法及試劑盒 HK1249133A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/106609 WO2018090373A1 (zh) 2016-11-21 2016-11-21 一种dna末端修复与加a的方法

Publications (1)

Publication Number Publication Date
HK1249133A1 true HK1249133A1 (zh) 2018-10-26

Family

ID=62146014

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18108558.7A HK1249133A1 (zh) 2016-11-21 2018-07-03 一種用於無創產前檢測的文庫構建方法及試劑盒

Country Status (3)

Country Link
CN (2) CN109689872B (zh)
HK (1) HK1249133A1 (zh)
WO (1) WO2018090373A1 (zh)

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CN110551794B (zh) * 2018-06-04 2023-05-30 完整基因有限公司 对rna分子进行处理的方法及试剂盒和复合体
CN109055518A (zh) * 2018-07-06 2018-12-21 天津华大医学检验所有限公司 用于常见遗传病检测的探针、基因芯片及制备方法和应用
CN110791813B (zh) * 2018-08-01 2023-06-16 广州华大基因医学检验所有限公司 对单链dna进行处理的方法及应用
CN109321567A (zh) * 2018-10-10 2019-02-12 菲鹏生物股份有限公司 测序用dna文库试剂盒以及测序用dna文库构建方法
EP3865584A4 (en) * 2018-10-11 2021-12-08 Beijing Euler Technology Limited Company PROCEDURE FOR CREATING A SEQUENCING LIBRARY
CN109610008A (zh) * 2018-11-08 2019-04-12 广州华大基因医学检验所有限公司 基于高通量测序的中枢***感染病原检测文库构建方法、检测方法和试剂盒
TW202039838A (zh) * 2018-12-28 2020-11-01 大陸商江蘇金斯瑞生物科技有限公司 一種單鏈dna合成方法
CN111073952B (zh) * 2018-12-29 2023-09-22 浙江安诺优达生物科技有限公司 构建dna文库的方法及其应用
CN109957605A (zh) * 2019-03-20 2019-07-02 嘉兴菲沙基因信息有限公司 适用于PacBio测序平台的海洋动物与软体动物的建库测序方法
CN109971827B (zh) * 2019-03-25 2020-05-01 纳昂达(南京)生物科技有限公司 血浆dna的建库方法和建库试剂盒
CN112176028B (zh) * 2019-07-05 2024-04-16 深圳华大生命科学研究院 一种基于核酸内切酶的快速wgs建库方法
CN112301103B (zh) * 2019-08-02 2023-04-11 北京贝瑞和康生物技术有限公司 一种非特异性扩增天然短片段核酸的方法和试剂盒
CN112342627A (zh) * 2019-08-09 2021-02-09 深圳市真迈生物科技有限公司 一种核酸文库的制备方法及测序方法
CN116144735A (zh) * 2019-08-12 2023-05-23 深圳市真迈生物科技有限公司 核酸样本处理方法、测序方法和试剂盒
CN112553298B (zh) * 2019-09-10 2023-06-23 深圳华大生命科学研究院 一种可以改善全基因组甲基化测序偏向性的建库方法
CN111139533B (zh) * 2019-09-27 2021-11-09 上海英基生物科技有限公司 稳定性增加的测序文库接头
CN110656156A (zh) * 2019-10-14 2020-01-07 湖南大地同年生物科技有限公司 一种超低频突变核酸片段检测方法、文库构建方法、引物设计方法和试剂
CN110846382B (zh) * 2019-11-29 2023-02-28 北京科迅生物技术有限公司 胎儿游离dna的富集方法
CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN111394799A (zh) * 2020-03-11 2020-07-10 广州赛哲生物科技股份有限公司 一种脑膜炎病原体宏基因组二代测序文库的构建方法及其试剂盒
CN111378632B (zh) * 2020-03-19 2021-03-19 江苏康为世纪生物科技股份有限公司 cfDNA末端修复的酶组合物、缓冲液试剂和测序文库的构建方法
CN113774120B (zh) * 2020-06-09 2024-05-14 深圳华大智造科技股份有限公司 Dnb双末端测序方法
WO2021253372A1 (zh) * 2020-06-19 2021-12-23 深圳华大智造科技有限公司 一种高兼容性的PCR-free建库和测序方法
CN111763664B (zh) * 2020-06-28 2021-03-26 江苏康科斯医疗科技有限公司 一种用于构建测序文库的酶反应液及其应用
CN112226821B (zh) * 2020-10-16 2024-02-06 鲲羽生物科技(江门)有限公司 一种基于双链环化的mgi测序平台测序文库的构建方法
CN112708939A (zh) * 2020-12-30 2021-04-27 深圳海普洛斯医学检验实验室 构建dna文库的试剂盒及方法
CN112708619B (zh) * 2020-12-30 2022-05-17 纳昂达(南京)生物科技有限公司 Mgi平台的建库用接头、试剂盒及建库方法
CN112779315A (zh) * 2021-01-04 2021-05-11 深圳海普洛斯医学检验实验室 一种基于Illumina测序平台的游离DNA文库构建用试剂盒及其应用
CN113355379A (zh) * 2021-02-04 2021-09-07 康龙化成(北京)新药技术股份有限公司 一种经济实用的核酸链5’-羟基磷酸化方法
CN113512766A (zh) * 2021-08-25 2021-10-19 广州达安基因股份有限公司 一种dna二代测序文库的构建方法
CN113832549A (zh) * 2021-11-03 2021-12-24 纳昂达(南京)生物科技有限公司 低频引入突变的酶切打断建库方法和试剂盒
CN115125292A (zh) * 2022-06-02 2022-09-30 武汉大学 一种内切酶辅助的rna中肌苷修饰的单碱基分辨率定位分析方法
CN114774411B (zh) * 2022-06-16 2022-10-21 序康医疗科技（苏州）有限公司 大片段dna环化连接方法
WO2024036445A1 (zh) * 2022-08-15 2024-02-22 深圳华大智造科技股份有限公司 一种测序文库的制备方法及用于制备测序文库的试剂盒

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CN102459592B (zh) * 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
CN102409042B (zh) * 2010-09-21 2014-05-14 深圳华大基因科技服务有限公司 一种高通量基因组甲基化dna富集方法及其所使用标签和标签接头
CN101967476B (zh) * 2010-09-21 2012-11-14 深圳华大基因科技有限公司 一种基于接头连接的DNA PCR-Free标签文库构建方法
CN103290104B (zh) * 2013-01-23 2016-03-02 北京诺禾致源生物信息科技有限公司 一种应用于第二代测序的简捷廉价的基因组样品破碎方法
CN105400864B (zh) * 2014-09-12 2020-04-14 深圳华大基因股份有限公司 用于基于血液样品构建测序文库的方法及其在确定胎儿遗传异常中的用途
WO2016037358A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
CN107124888B (zh) * 2014-11-21 2021-08-06 深圳华大智造科技股份有限公司 鼓泡状接头元件和使用其构建测序文库的方法
WO2016078096A1 (zh) * 2014-11-21 2016-05-26 深圳华大基因科技有限公司 使用鼓泡状接头元件构建测序文库的方法
CN105603535B (zh) * 2016-01-27 2018-11-27 北京诺禾致源科技股份有限公司 构建dna文库的试剂盒和方法

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CN108085315A (zh) 2018-05-29
CN109689872B (zh) 2022-12-23
CN109689872A (zh) 2019-04-26
WO2018090373A1 (zh) 2018-05-24

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