HK1220735A1 - Multiplex pcr primers for detection of -thalassemia mutation based on next-generation sequencing technology and method and application thereof - pcr - Google Patents

Multiplex pcr primers for detection of -thalassemia mutation based on next-generation sequencing technology and method and application thereof - pcr

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Publication number
HK1220735A1
HK1220735A1 HK16108731.9A HK16108731A HK1220735A1 HK 1220735 A1 HK1220735 A1 HK 1220735A1 HK 16108731 A HK16108731 A HK 16108731A HK 1220735 A1 HK1220735 A1 HK 1220735A1
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HK
Hong Kong
Prior art keywords
detection
application
pcr
generation sequencing
sequencing technology
Prior art date
Application number
HK16108731.9A
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Chinese (zh)
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Publication date
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Publication of HK1220735A1 publication Critical patent/HK1220735A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
HK16108731.9A 2015-11-04 2016-07-20 Multiplex pcr primers for detection of -thalassemia mutation based on next-generation sequencing technology and method and application thereof - pcr HK1220735A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510740909.5A CN105331694B (en) 2015-11-04 2015-11-04 It is a kind of to detect β-thalassaemia mutations multiple PCR primer and method and application based on next-generation sequencing technologies

Publications (1)

Publication Number Publication Date
HK1220735A1 true HK1220735A1 (en) 2017-05-12

Family

ID=55282457

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16108731.9A HK1220735A1 (en) 2015-11-04 2016-07-20 Multiplex pcr primers for detection of -thalassemia mutation based on next-generation sequencing technology and method and application thereof - pcr

Country Status (3)

Country Link
CN (5) CN106868187A (en)
HK (1) HK1220735A1 (en)
WO (1) WO2017076299A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106868187A (en) * 2015-11-04 2017-06-20 深圳市瀚海基因生物科技有限公司 Multiple PCR primer, kit and purposes
CN105886617A (en) * 2016-04-16 2016-08-24 广州市达瑞生物技术股份有限公司 Thalassemia gene detection method based on high-throughput sequencing technology
CN109112197A (en) * 2017-06-23 2019-01-01 陈治中 A kind of chip, amplifing reagent and kit directly detecting α and beta Thalassemia simultaneously
CN109112198A (en) * 2017-06-23 2019-01-01 陈治中 It is a kind of for quickly detecting chip, amplifing reagent and the kit of 21 kinds of point mutation beta Thalassemia allele
CN107365846B (en) * 2017-08-04 2020-04-14 亚能生物技术（深圳）有限公司 Kit for rapidly detecting common β thalassemia mutation allele of Chinese population
CN107267648B (en) * 2017-08-04 2020-04-14 亚能生物技术（深圳）有限公司 Kit for rapidly detecting common mutant genes of thalassemia
CN107245528B (en) * 2017-08-04 2020-04-21 亚能生物技术（深圳）有限公司 Rapid detection kit for common thalassemia mutant genes of Chinese population
CN107988320A (en) * 2017-11-10 2018-05-04 至本医疗科技（上海）有限公司 A kind of molecular label connector and its preparation method and application
CN108796042B (en) * 2018-05-23 2022-04-05 北昊干细胞与再生医学研究院有限公司 Nucleic acid composition for genetic anemia detection, detection kit and use method
CN109182526A (en) * 2018-10-10 2019-01-11 杭州翱锐生物科技有限公司 Kit and its detection method for early liver cancer auxiliary diagnosis
CN110628895A (en) * 2019-10-16 2019-12-31 重庆市人口和计划生育科学技术研究院 Method for screening multiple genetic disease SNPs (Single nucleotide polymorphisms) based on nucleic acid flight mass spectrometry and primers used in method
CN111471752A (en) * 2020-03-27 2020-07-31 广州辉园苑医药科技有限公司 Primer and kit for detecting HBB gene locus mutation
CN114686579B (en) * 2020-12-28 2024-04-30 广东菲鹏生物有限公司 Compositions, kits, methods and systems for nucleic acid sample amplification
CN114686580B (en) * 2020-12-28 2024-04-30 广东菲鹏生物有限公司 Compositions, kits, methods and systems for nucleic acid sample amplification
CN114686562B (en) * 2020-12-28 2024-04-30 广东菲鹏生物有限公司 Compositions, kits, methods and systems for nucleic acid sample amplification
CN114686561B (en) * 2020-12-28 2024-04-30 广东菲鹏生物有限公司 Compositions, kits, methods and systems for nucleic acid sample amplification
CN113981059B (en) * 2021-06-29 2023-08-22 上海真固生物科技有限公司 Primer composition for detecting thalassemia mutant gene and reagent thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5940458B2 (en) * 2010-01-12 2016-06-29 アーラム バイオシステムズ インコーポレイテッド Three-stage thermal convection device and method of use
CN102409100B (en) * 2011-11-29 2014-06-04 潮州市中心医院 Real-time fluorescence quantitative PCR(Polymerase Chain Reaction) detection primers and kit for thalassemia
CN103255225B (en) * 2013-05-27 2014-09-10 钦州市妇幼保健院 Thalassemia gene detection method based on fluorescence labeling quantitation PCR (Polymerase Chain Reaction) technology
CN103602752B (en) * 2013-12-06 2015-03-04 亚能生物技术（深圳）有限公司 Primer set and kit for detecting rare deletion type thalassemia
CN104212910B (en) * 2014-09-28 2016-02-17 广东凯普生物科技股份有限公司 Single tube amplification detects α and beta Thalassemia kit gene simultaneously
CN104293937B (en) * 2014-09-28 2016-12-07 广东省妇幼保健院 One group of liquid-phase chip based on lock nucleic acid enhanced sensitivity detects the probe of thalassemia point mutation, detection kit and detection method
CN104894279B (en) * 2015-06-25 2016-10-19 北京嘉宝仁和医疗科技有限公司 Gene of alpha thalassemia mutation detection kit
CN106868187A (en) * 2015-11-04 2017-06-20 深圳市瀚海基因生物科技有限公司 Multiple PCR primer, kit and purposes

Also Published As

Publication number Publication date
CN106834530A (en) 2017-06-13
CN106868187A (en) 2017-06-20
WO2017076299A1 (en) 2017-05-11
CN107058307A (en) 2017-08-18
CN105331694A (en) 2016-02-17
CN107043768A (en) 2017-08-15
CN105331694B (en) 2019-05-07

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