GR20020100196A - Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j - Google Patents

Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j

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Publication number
GR20020100196A
GR20020100196A GR20020100196A GR2002100196A GR20020100196A GR 20020100196 A GR20020100196 A GR 20020100196A GR 20020100196 A GR20020100196 A GR 20020100196A GR 2002100196 A GR2002100196 A GR 2002100196A GR 20020100196 A GR20020100196 A GR 20020100196A
Authority
GR
Greece
Prior art keywords
serum levels
enzyme
clusterin
apolipoprotein
kit
Prior art date
Application number
GR20020100196A
Other languages
Greek (el)
Other versions
GR1004477B (en
Inventor
Ευσταθιος Γκονος
Ιωαννης Τρουγκακος
Original Assignee
Ευσταθιος Γκονος
Ιωαννης Τρουγκακος
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ευσταθιος Γκονος, Ιωαννης Τρουγκακος filed Critical Ευσταθιος Γκονος
Priority to GR20020100196A priority Critical patent/GR1004477B/en
Publication of GR20020100196A publication Critical patent/GR20020100196A/en
Publication of GR1004477B publication Critical patent/GR1004477B/en

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Abstract

his invention combines various technologies and renders the relative quantitative measurement of the of serum levels of Clusterin/Apolipoprotein J. It concerns a variation - adaptation of the ELISA methodology using two antibodies of which the second is conjugated chemically to the enzyme horseradish peroxidase (HRP, tracing enzyme) via which, by chromatic reaction, the serum levels of ApoJ are detected. Alternatively, it is possible that the second antibody does not bear the HRP enzyme conjugated to it, in which case, a third antibody is used against the Fc region of the species where the second antibody against ApoJ has been developed, which bears the HRP enzyme conjugated to it. The kit may be used for the prognosis in patients with diabetes type II or heart coronary disease, for the detection of vascular damage.
GR20020100196A 2002-04-23 2002-04-23 Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j GR1004477B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20020100196A GR1004477B (en) 2002-04-23 2002-04-23 Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20020100196A GR1004477B (en) 2002-04-23 2002-04-23 Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j

Publications (2)

Publication Number Publication Date
GR20020100196A true GR20020100196A (en) 2003-12-22
GR1004477B GR1004477B (en) 2004-03-08

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ID=29764772

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20020100196A GR1004477B (en) 2002-04-23 2002-04-23 Method and kit for the quantitave measurement of serum levels of clusterin/apolipoprotein j

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GR (1) GR1004477B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104280552A (en) * 2013-07-12 2015-01-14 张曼 Application of urine apolipoprotein J precursor protein

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4722893A (en) * 1983-07-06 1988-02-02 Shiraimatsu Shinyaku Kabushiki Kaisha Reagents for enzyme immunoassay for apolipoprotein B
EP0301667A1 (en) * 1987-07-28 1989-02-01 Istituto Sieroterapico Milanese "S. Belfanti" Method for the immunoassay of apolipoproteins
JPH0244253A (en) * 1988-08-04 1990-02-14 Tosoh Corp Measurement of apolipoprotein a-i
EP0621284A1 (en) * 1992-08-14 1994-10-26 Shino-Test Corporation PEPTIDES CONTAINING RESPECTIVE AMINO ACID SEQUENCES SELECTED FROM AMONG THOSE OF LIPOPROTEIN(a) AND APOLIPOPROTEIN(a), ANTIBODIES RESPECTIVELY RECOGNIZING THESE AMINO ACID SEQUENCES, AND METHOD OF ASSAYING WITH THESE ANTIBODIES
WO1996032502A1 (en) * 1995-04-11 1996-10-17 The Trustees Of Columbia University In The City Of New York Reagent specific for apolipoprotein-j polymorphisms and uses thereof
WO1999004260A1 (en) * 1997-07-15 1999-01-28 Bioprobes, Inc. Determining alcohol intake using sialic acid/apo j
US6197596B1 (en) * 1997-12-04 2001-03-06 Mcgill University Monitoring and/or prognostic of antibody-mediated autoimmune diseases

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4722893A (en) * 1983-07-06 1988-02-02 Shiraimatsu Shinyaku Kabushiki Kaisha Reagents for enzyme immunoassay for apolipoprotein B
EP0301667A1 (en) * 1987-07-28 1989-02-01 Istituto Sieroterapico Milanese "S. Belfanti" Method for the immunoassay of apolipoproteins
JPH0244253A (en) * 1988-08-04 1990-02-14 Tosoh Corp Measurement of apolipoprotein a-i
EP0621284A1 (en) * 1992-08-14 1994-10-26 Shino-Test Corporation PEPTIDES CONTAINING RESPECTIVE AMINO ACID SEQUENCES SELECTED FROM AMONG THOSE OF LIPOPROTEIN(a) AND APOLIPOPROTEIN(a), ANTIBODIES RESPECTIVELY RECOGNIZING THESE AMINO ACID SEQUENCES, AND METHOD OF ASSAYING WITH THESE ANTIBODIES
WO1996032502A1 (en) * 1995-04-11 1996-10-17 The Trustees Of Columbia University In The City Of New York Reagent specific for apolipoprotein-j polymorphisms and uses thereof
WO1999004260A1 (en) * 1997-07-15 1999-01-28 Bioprobes, Inc. Determining alcohol intake using sialic acid/apo j
US6197596B1 (en) * 1997-12-04 2001-03-06 Mcgill University Monitoring and/or prognostic of antibody-mediated autoimmune diseases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 014, no. 204 (P - 1042) 25 April 1990 (1990-04-25) *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104280552A (en) * 2013-07-12 2015-01-14 张曼 Application of urine apolipoprotein J precursor protein

Also Published As

Publication number Publication date
GR1004477B (en) 2004-03-08

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