GR1005420B - Μιμητικα κυτταρα - Google Patents

Μιμητικα κυτταρα

Info

Publication number
GR1005420B
GR1005420B GR20060100036A GR20060100036A GR1005420B GR 1005420 B GR1005420 B GR 1005420B GR 20060100036 A GR20060100036 A GR 20060100036A GR 20060100036 A GR20060100036 A GR 20060100036A GR 1005420 B GR1005420 B GR 1005420B
Authority
GR
Greece
Prior art keywords
molecules
cells
measurement
determination
under determination
Prior art date
Application number
GR20060100036A
Other languages
English (en)
Inventor
Σπυριδων Ευαγγελου Κιντζιος
Original Assignee
Σπυριδων Ευαγγελου Κιντζιος
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Σπυριδων Ευαγγελου Κιντζιος filed Critical Σπυριδων Ευαγγελου Κιντζιος
Priority to GR20060100036A priority Critical patent/GR1005420B/el
Priority to US12/086,966 priority patent/US20090170068A1/en
Priority to EP07700431.5A priority patent/EP1974211B1/en
Priority to PCT/GR2007/000004 priority patent/WO2007083170A1/en
Publication of GR1005420B publication Critical patent/GR1005420B/el

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Η παρούσα εφεύρεση αναφέρεται στην ανάπτυξη αναλυτικών συσκευών βασισμένων σε ένα ή περισσότερα κύτταρα (κυτταρικών βιοαισθητήρων) των οποίων η επιφάνεια έχει τροποποιηθεί με την τεχνητή εισαγωγή μορίων κατά τέτοιο τρόπο ώστε να αντιδρούν εκλεκτικά με τις ουσίες υπό ανίχνευση ("μιμητικά κύτταρα"). Αυτά τα μόρια-υποδοχείς μπορεί να είναι πρωτεϊνες( όπως ένζυμα, αντιγόνα ή αντισώματα), νουκλεϊκάοξέα, υδατάνθρακες, λιπίδια ή να ανήκουν σε οποιαδήποτε άλλη χημική ομάδα με ικανότητα εκλεκτικής αλληλεπίδρασης με ουσίες-στόχους ("αναλύτες") οι οποίες είναι επιθυμητό να ανιχνευθούν σε ένα ή περισσότερα δείγματα. Η εισαγωγή των μορίων αυτών στην επιφάνεια των κυττάρων μπορεί να πραγματοποιηθεί μέσω ηλακτροεισαγωγής ή άλλης κατάλληλης μεθόδου. Διαφορετικές κατηγορίες μορίων μπορούν να εισαχθούνστη μεμβράνη του ίδιου κυττάρου, ιδιαίτερα αν αυτό εξυπηρετεί την εκλεκτικότητα αλληλεπίδρασης με τουςυπό προσδιορισμό παράγοντες. Η μέθοδος περιλαμβάνει τη χρήση κατάλληλου βιοαισθητήρα ο οποίος περιλαμβάνει το τροποποιημένο κυτταρικό υλικό ελεύθερο ή ακινητοποιημένο μέσα σε πλέγμα ή πάνω σε υπόστρωμα από κατάλληλο υλικό, έτσι ώστε να εξασφαλίζεταιη μέτρηση της εκλεκτικής αντίδρασης με τον υπόπροσδιορισμό παράγοντα. Η μέτρηση της αντίδρασης μπορεί να πραγματοποιηθεί με οποιαδήποτε κατάλληλη μέθοδο αναφερόμενη σε φυσικοχημική ιδιότητα του αισθητήρα, όπως τη μέτρηση της μεταβολής του δυναμικού ή διαφόρων οπτικών ιδιοτήτων (όπως φθορισμός, φωταύγεια, χημειοφωταύγεια ή ηλεκτροχημειοφωταύγεια). Επομένως είναι δυνατός ο προσδιορισμός ενός χημικού ή βιλογικού παράγοντα εφ'όσον είναι γνωστή ηκαμπύλη μεταβολής μίας ορισμένης φυσικοχημικής ιδιότητας του βιοαισθητήρα στις διάφορες συγκετρώσεις τουπαράγοντα αυτού και σε σχέσημε άλλους παρεμφερούςδομής ή δράσης παράγοντες.
GR20060100036A 2006-01-20 2006-01-20 Μιμητικα κυτταρα GR1005420B (el)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GR20060100036A GR1005420B (el) 2006-01-20 2006-01-20 Μιμητικα κυτταρα
US12/086,966 US20090170068A1 (en) 2006-01-20 2007-01-18 Molecular Identification Through Membrane-Engineered Cells
EP07700431.5A EP1974211B1 (en) 2006-01-20 2007-01-18 Molecular identification through membrane-engineered cells
PCT/GR2007/000004 WO2007083170A1 (en) 2006-01-20 2007-01-18 Molecular identification through membrane-engineered cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20060100036A GR1005420B (el) 2006-01-20 2006-01-20 Μιμητικα κυτταρα

Publications (1)

Publication Number Publication Date
GR1005420B true GR1005420B (el) 2007-01-31

Family

ID=37998387

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20060100036A GR1005420B (el) 2006-01-20 2006-01-20 Μιμητικα κυτταρα

Country Status (4)

Country Link
US (1) US20090170068A1 (el)
EP (1) EP1974211B1 (el)
GR (1) GR1005420B (el)
WO (1) WO2007083170A1 (el)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1327174C (en) * 1988-10-05 1994-02-22 Youssef Mouneimne Electro-insertion of proteins into animal cell membranes
US5789152A (en) * 1991-04-30 1998-08-04 Basil T. Hone Composition and method for detecting HIV with baculovirus derived vesicles
US6248542B1 (en) * 1997-12-09 2001-06-19 Massachusetts Institute Of Technology Optoelectronic sensor
GR1003489B (el) * 1999-11-19 2000-11-30 Μεθοδος προσδιορισμου βιολογικων και χημικων παραγοντων μεσω της μετρησης των μεταβολων των ηλεκτρικων ιδιοτητων ακινητοποιημενων κυτταρων ή συστατικων αυτων (βιοηλεκτρικη μεθοδος αναγνωρισης)
ATE506452T1 (de) * 2001-02-07 2011-05-15 Massachusetts Inst Technology Optoelektronisches detektionssystem
WO2003050524A1 (en) * 2001-05-23 2003-06-19 Spiridon Kintzios Method for identifying biochemical material

Also Published As

Publication number Publication date
EP1974211B1 (en) 2017-09-20
US20090170068A1 (en) 2009-07-02
WO2007083170A1 (en) 2007-07-26
WO2007083170B1 (en) 2007-10-18
EP1974211A1 (en) 2008-10-01

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