GR1005420B - Μιμητικα κυτταρα - Google Patents
Μιμητικα κυτταραInfo
- Publication number
- GR1005420B GR1005420B GR20060100036A GR20060100036A GR1005420B GR 1005420 B GR1005420 B GR 1005420B GR 20060100036 A GR20060100036 A GR 20060100036A GR 20060100036 A GR20060100036 A GR 20060100036A GR 1005420 B GR1005420 B GR 1005420B
- Authority
- GR
- Greece
- Prior art keywords
- molecules
- cells
- measurement
- determination
- under determination
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5082—Supracellular entities, e.g. tissue, organisms
- G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Η παρούσα εφεύρεση αναφέρεται στην ανάπτυξη αναλυτικών συσκευών βασισμένων σε ένα ή περισσότερα κύτταρα (κυτταρικών βιοαισθητήρων) των οποίων η επιφάνεια έχει τροποποιηθεί με την τεχνητή εισαγωγή μορίων κατά τέτοιο τρόπο ώστε να αντιδρούν εκλεκτικά με τις ουσίες υπό ανίχνευση ("μιμητικά κύτταρα"). Αυτά τα μόρια-υποδοχείς μπορεί να είναι πρωτεϊνες( όπως ένζυμα, αντιγόνα ή αντισώματα), νουκλεϊκάοξέα, υδατάνθρακες, λιπίδια ή να ανήκουν σε οποιαδήποτε άλλη χημική ομάδα με ικανότητα εκλεκτικής αλληλεπίδρασης με ουσίες-στόχους ("αναλύτες") οι οποίες είναι επιθυμητό να ανιχνευθούν σε ένα ή περισσότερα δείγματα. Η εισαγωγή των μορίων αυτών στην επιφάνεια των κυττάρων μπορεί να πραγματοποιηθεί μέσω ηλακτροεισαγωγής ή άλλης κατάλληλης μεθόδου. Διαφορετικές κατηγορίες μορίων μπορούν να εισαχθούνστη μεμβράνη του ίδιου κυττάρου, ιδιαίτερα αν αυτό εξυπηρετεί την εκλεκτικότητα αλληλεπίδρασης με τουςυπό προσδιορισμό παράγοντες. Η μέθοδος περιλαμβάνει τη χρήση κατάλληλου βιοαισθητήρα ο οποίος περιλαμβάνει το τροποποιημένο κυτταρικό υλικό ελεύθερο ή ακινητοποιημένο μέσα σε πλέγμα ή πάνω σε υπόστρωμα από κατάλληλο υλικό, έτσι ώστε να εξασφαλίζεταιη μέτρηση της εκλεκτικής αντίδρασης με τον υπόπροσδιορισμό παράγοντα. Η μέτρηση της αντίδρασης μπορεί να πραγματοποιηθεί με οποιαδήποτε κατάλληλη μέθοδο αναφερόμενη σε φυσικοχημική ιδιότητα του αισθητήρα, όπως τη μέτρηση της μεταβολής του δυναμικού ή διαφόρων οπτικών ιδιοτήτων (όπως φθορισμός, φωταύγεια, χημειοφωταύγεια ή ηλεκτροχημειοφωταύγεια). Επομένως είναι δυνατός ο προσδιορισμός ενός χημικού ή βιλογικού παράγοντα εφ'όσον είναι γνωστή ηκαμπύλη μεταβολής μίας ορισμένης φυσικοχημικής ιδιότητας του βιοαισθητήρα στις διάφορες συγκετρώσεις τουπαράγοντα αυτού και σε σχέσημε άλλους παρεμφερούςδομής ή δράσης παράγοντες.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GR20060100036A GR1005420B (el) | 2006-01-20 | 2006-01-20 | Μιμητικα κυτταρα |
US12/086,966 US20090170068A1 (en) | 2006-01-20 | 2007-01-18 | Molecular Identification Through Membrane-Engineered Cells |
EP07700431.5A EP1974211B1 (en) | 2006-01-20 | 2007-01-18 | Molecular identification through membrane-engineered cells |
PCT/GR2007/000004 WO2007083170A1 (en) | 2006-01-20 | 2007-01-18 | Molecular identification through membrane-engineered cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GR20060100036A GR1005420B (el) | 2006-01-20 | 2006-01-20 | Μιμητικα κυτταρα |
Publications (1)
Publication Number | Publication Date |
---|---|
GR1005420B true GR1005420B (el) | 2007-01-31 |
Family
ID=37998387
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GR20060100036A GR1005420B (el) | 2006-01-20 | 2006-01-20 | Μιμητικα κυτταρα |
Country Status (4)
Country | Link |
---|---|
US (1) | US20090170068A1 (el) |
EP (1) | EP1974211B1 (el) |
GR (1) | GR1005420B (el) |
WO (1) | WO2007083170A1 (el) |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1327174C (en) * | 1988-10-05 | 1994-02-22 | Youssef Mouneimne | Electro-insertion of proteins into animal cell membranes |
US5789152A (en) * | 1991-04-30 | 1998-08-04 | Basil T. Hone | Composition and method for detecting HIV with baculovirus derived vesicles |
US6248542B1 (en) * | 1997-12-09 | 2001-06-19 | Massachusetts Institute Of Technology | Optoelectronic sensor |
GR1003489B (el) * | 1999-11-19 | 2000-11-30 | Μεθοδος προσδιορισμου βιολογικων και χημικων παραγοντων μεσω της μετρησης των μεταβολων των ηλεκτρικων ιδιοτητων ακινητοποιημενων κυτταρων ή συστατικων αυτων (βιοηλεκτρικη μεθοδος αναγνωρισης) | |
ATE506452T1 (de) * | 2001-02-07 | 2011-05-15 | Massachusetts Inst Technology | Optoelektronisches detektionssystem |
WO2003050524A1 (en) * | 2001-05-23 | 2003-06-19 | Spiridon Kintzios | Method for identifying biochemical material |
-
2006
- 2006-01-20 GR GR20060100036A patent/GR1005420B/el active IP Right Grant
-
2007
- 2007-01-18 WO PCT/GR2007/000004 patent/WO2007083170A1/en active Application Filing
- 2007-01-18 EP EP07700431.5A patent/EP1974211B1/en active Active
- 2007-01-18 US US12/086,966 patent/US20090170068A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1974211B1 (en) | 2017-09-20 |
US20090170068A1 (en) | 2009-07-02 |
WO2007083170A1 (en) | 2007-07-26 |
WO2007083170B1 (en) | 2007-10-18 |
EP1974211A1 (en) | 2008-10-01 |
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