GEP19971033B - Method of preparing lysozyme dimers - Google Patents

Method of preparing lysozyme dimers

Info

Publication number
GEP19971033B
GEP19971033B GEAP1993760A GEAP1993000760A GEP19971033B GE P19971033 B GEP19971033 B GE P19971033B GE AP1993760 A GEAP1993760 A GE AP1993760A GE AP1993000760 A GEAP1993000760 A GE AP1993000760A GE P19971033 B GEP19971033 B GE P19971033B
Authority
GE
Georgia
Prior art keywords
lysozyme
dimer
monomer
dimerization
preparing
Prior art date
Application number
GEAP1993760A
Inventor
Peter Herrmann
Original Assignee
Nika Health Products Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1990/000140 external-priority patent/WO1991010731A1/en
Application filed by Nika Health Products Ltd filed Critical Nika Health Products Ltd
Publication of GEP19971033B publication Critical patent/GEP19971033B/en

Links

Landscapes

  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out using ion exchange resin column chromatography. Monomeric lysozyme remaining undimerized by the initial dimerization step is recycled into the process in order to increase yield. The present system is advantageous in that large amounts of purified lysozyme dimer can be prepared efficiently and relatively inexpensively, and the lysozyme dimer so produced is useful in treating viral and/or bacterial diseases without causing the cytotoxic effects associated with the lysozyme monomer.
GEAP1993760A 1990-01-08 1993-05-19 Method of preparing lysozyme dimers GEP19971033B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1990/000140 WO1991010731A1 (en) 1990-01-08 1990-01-08 Method of preparing lysozyme dimers

Publications (1)

Publication Number Publication Date
GEP19971033B true GEP19971033B (en) 1997-12-11

Family

ID=82484076

Family Applications (1)

Application Number Title Priority Date Filing Date
GEAP1993760A GEP19971033B (en) 1990-01-08 1993-05-19 Method of preparing lysozyme dimers

Country Status (1)

Country Link
GE (1) GEP19971033B (en)

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