GEP19971033B - Method of preparing lysozyme dimers - Google Patents
Method of preparing lysozyme dimersInfo
- Publication number
- GEP19971033B GEP19971033B GEAP1993760A GEAP1993000760A GEP19971033B GE P19971033 B GEP19971033 B GE P19971033B GE AP1993760 A GEAP1993760 A GE AP1993760A GE AP1993000760 A GEAP1993000760 A GE AP1993000760A GE P19971033 B GEP19971033 B GE P19971033B
- Authority
- GE
- Georgia
- Prior art keywords
- lysozyme
- dimer
- monomer
- dimerization
- preparing
- Prior art date
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out using ion exchange resin column chromatography. Monomeric lysozyme remaining undimerized by the initial dimerization step is recycled into the process in order to increase yield. The present system is advantageous in that large amounts of purified lysozyme dimer can be prepared efficiently and relatively inexpensively, and the lysozyme dimer so produced is useful in treating viral and/or bacterial diseases without causing the cytotoxic effects associated with the lysozyme monomer.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1990/000140 WO1991010731A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing lysozyme dimers |
Publications (1)
Publication Number | Publication Date |
---|---|
GEP19971033B true GEP19971033B (en) | 1997-12-11 |
Family
ID=82484076
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GEAP1993760A GEP19971033B (en) | 1990-01-08 | 1993-05-19 | Method of preparing lysozyme dimers |
Country Status (1)
Country | Link |
---|---|
GE (1) | GEP19971033B (en) |
-
1993
- 1993-05-19 GE GEAP1993760A patent/GEP19971033B/en unknown
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IL117320A0 (en) | Process for producing a protein | |
DE69434532D1 (en) | Process for the preparation of a high trehalose content product | |
EP0325438B1 (en) | Two-stage process for converson of alkanes to gasoline | |
MY103892A (en) | Interleukin-2 of high purity production and use | |
ES8706101A1 (en) | Process for the preparation of dimerization products from olefins. | |
MX9703727A (en) | Method of producing caprolactam. | |
CA2073351A1 (en) | Lysozyme dimers and method of preparing them | |
ATE131490T1 (en) | METHOD FOR PRODUCING PURIFIED ALBUM SOLUTIONS | |
GEP19971033B (en) | Method of preparing lysozyme dimers | |
OA09707A (en) | Method of preparing ribonuclease dimers | |
DE69212072D1 (en) | Continuous process for the production of sucrose 6 esters | |
GEP19971022B (en) | Method of preparing ribonuclease dimers | |
HUT53377A (en) | Process for producing antibiotic l 17392 (deglucoteicoplanin) and salts thereof | |
CA2073350A1 (en) | Ribonuclease dimers and method of preparing them | |
IL73370A (en) | Process for the purification of dihydroxybenzophenones | |
WO1994004017A3 (en) | New method of treatment | |
MC1627A1 (en) | PROCESS FOR PRODUCING AN IMMUN INTERFERON | |
GEP19960524B (en) | Method of purifying fibrinous interferon of human being | |
JPS6419052A (en) | Production of l-methylosin | |
GB1464647A (en) | Process for the preparation of choleragen | |
ES8302627A1 (en) | 5-Acetyloxy-L-tryptophane and its salts, process for their preparation and their therapeutical uses. | |
ATE77240T1 (en) | FRACTION OF LISTERIA MONOCYTOGENES WITH THERAPEUTIC ACTIVITY, PROCESS FOR THEIR PRODUCTION AND PHARMACEUTICAL COMPOSITION CONTAINING THEM. |