GB995362A - Improvements in or relating to freeze-drying biological material - Google Patents
Improvements in or relating to freeze-drying biological materialInfo
- Publication number
- GB995362A GB995362A GB18284/62A GB1828462A GB995362A GB 995362 A GB995362 A GB 995362A GB 18284/62 A GB18284/62 A GB 18284/62A GB 1828462 A GB1828462 A GB 1828462A GB 995362 A GB995362 A GB 995362A
- Authority
- GB
- United Kingdom
- Prior art keywords
- freeze
- drying
- drying medium
- dried
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000012620 biological material Substances 0.000 title abstract 7
- 238000004108 freeze drying Methods 0.000 title abstract 5
- 238000001035 drying Methods 0.000 abstract 12
- 241000894006 Bacteria Species 0.000 abstract 8
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 abstract 6
- 239000001768 carboxy methyl cellulose Substances 0.000 abstract 6
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 abstract 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 abstract 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 4
- 229930006000 Sucrose Natural products 0.000 abstract 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 4
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 abstract 4
- 235000013923 monosodium glutamate Nutrition 0.000 abstract 4
- 229940073490 sodium glutamate Drugs 0.000 abstract 4
- 239000005720 sucrose Substances 0.000 abstract 4
- 241000607132 Salmonella enterica subsp. enterica serovar Gallinarum Species 0.000 abstract 2
- 208000037386 Typhoid Diseases 0.000 abstract 2
- 241000700605 Viruses Species 0.000 abstract 2
- 239000007864 aqueous solution Substances 0.000 abstract 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract 2
- 239000003223 protective agent Substances 0.000 abstract 2
- 239000011369 resultant mixture Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- DLYUQMMRRRQYAE-UHFFFAOYSA-N tetraphosphorus decaoxide Chemical compound O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 abstract 2
- 231100000331 toxic Toxicity 0.000 abstract 2
- 230000002588 toxic effect Effects 0.000 abstract 2
- 201000008297 typhoid fever Diseases 0.000 abstract 2
- 229960005486 vaccine Drugs 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
Living or killed biological material such as bacteria, viruses, fowl-typhoid and B.C.G. vaccines is mixed with a drying medium comprising an aqueous solution of sodium carboxymethylcellulose and the resultant mixture is freeze-dried. The drying medium preferably includes in the case of living bacteria &c. from 2-10% by weight based on the total volume of the drying medium, of an inert substance capable of retaining a residual amount of water, e.g. a sugar such as sucrose, and from 0.5-10% of a protective agent such as sodium glutamate (thought to act against carbonyl groups toxic to bacteria in a dried state). The sodium carboxymethylcellulose should be one whose aqueous 1% w/v solution has a viscosity in the range 5-55 centipoises at 20 DEG C. and is employed in a concentration of from 0.5-2.0% by weight based on the total volume of the drying medium. The biological material is placed in vials or ampoules, is cooled to a temperature within the range -5 to -45 DEG C. and then freeze-dried to a moisture content below 1.2% w/w; secondary drying over phosphorous pentoxide may be additionally utilized. The freeze-dried biological material may be sealed in oxygen-free nitrogen or under vacuum. Examples are directed to freeze-drying live cells of a strain designated 9R of Salmonella gallinarum suspended in a drying medium containing between 0.5% and 2.0% w/v of sodium carboxymethylcellulose, between 2% and 10% w/v of sucrose and between 0.5% and 10% w/v of sodium glutamate. Between 20% and 35% of the live bacteria cells survived the freeze-drying process according to the various examples.ALSO:Living or killed biological material such as bacteria, viruses, fowl-typhoid and B.C.G. vaccines is mixed with a drying medium comprising an aqueous solution of sodium carboxymethylcellulose and the resultant mixture is freeze-dried. The drying medium preferably includes in the case of living bacteria &c. from 2 to 10% by weight based on the total volume of the drying medium, of an inert substance capable of retaining a residual amount of water e.g. a sugar such as sucrose, and from 0.5 to 10% of a protective agent such as sodium glutamate (thought to act against carbonyl groups toxic to bacteria in a dried state). The sodium carboxymethylcellulose should be one whose aqueous 1% w/v solution has a viscosity in the range 5-55 centipoises at 20 DEG C., and is employed in a concentration of from 0.5 to 2.0% by weight based on the total volume of the drying medium. The biological material is placed in vials or ampoules, is cooled to a temperature within the range -5 to -45 DEG C. and then freeze-dried to a moisture content below 1.2% w/w; secondary drying over phosphorous pentoxide may be additionally utilised. The freeze-dried biological material may be sealed in oxygen-free nitrogen or under vacuum. Examples are directed to freeze-drying live cells of a strain designated 9R of Salmonella gallinarum suspended in a drying medium containing between 0.5 and 2% w/v of sodium carboxymethylcellulose, between 2 and 10% w/v of sucrose and between 0.5 and 10 w/v of sodium glutamate. Between 20 and 35% of the live bacteria cells survived the freeze-drying process according to the various examples.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB18284/62A GB995362A (en) | 1962-05-11 | 1962-05-11 | Improvements in or relating to freeze-drying biological material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB18284/62A GB995362A (en) | 1962-05-11 | 1962-05-11 | Improvements in or relating to freeze-drying biological material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB995362A true GB995362A (en) | 1965-06-16 |
Family
ID=10109823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB18284/62A Expired GB995362A (en) | 1962-05-11 | 1962-05-11 | Improvements in or relating to freeze-drying biological material |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB995362A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2748132A1 (en) * | 1977-10-27 | 1979-05-03 | Behringwerke Ag | STABILIZER FOR POLYSACCHARIDE |
| EP0523130B1 (en) * | 1990-03-24 | 1997-11-12 | ENVIRONMENTAL & MEDICAL PRODUCTS LTD | Enzyme stabilisation |
-
1962
- 1962-05-11 GB GB18284/62A patent/GB995362A/en not_active Expired
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2748132A1 (en) * | 1977-10-27 | 1979-05-03 | Behringwerke Ag | STABILIZER FOR POLYSACCHARIDE |
| FR2406999A1 (en) * | 1977-10-27 | 1979-05-25 | Behringwerke Ag | STABILIZER FOR MENINGOCOCCAL POLYSACCHARIDES |
| EP0523130B1 (en) * | 1990-03-24 | 1997-11-12 | ENVIRONMENTAL & MEDICAL PRODUCTS LTD | Enzyme stabilisation |
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