GB9913694D0 - DNA manipulation methods and applications for synthetic enzymes - Google Patents

DNA manipulation methods and applications for synthetic enzymes

Info

Publication number
GB9913694D0
GB9913694D0 GBGB9913694.7A GB9913694A GB9913694D0 GB 9913694 D0 GB9913694 D0 GB 9913694D0 GB 9913694 A GB9913694 A GB 9913694A GB 9913694 D0 GB9913694 D0 GB 9913694D0
Authority
GB
United Kingdom
Prior art keywords
applications
dna manipulation
manipulation methods
synthetic enzymes
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB9913694.7A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SIMPER ADRIAN M
Original Assignee
SIMPER ADRIAN M
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SIMPER ADRIAN M filed Critical SIMPER ADRIAN M
Priority to GBGB9913694.7A priority Critical patent/GB9913694D0/en
Publication of GB9913694D0 publication Critical patent/GB9913694D0/en
Priority to AU55457/00A priority patent/AU5545700A/en
Priority to PCT/GB2000/002286 priority patent/WO2000077181A2/en
Priority to EP00940533A priority patent/EP1190045A2/en
Priority to CA002376559A priority patent/CA2376559A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/08—Oxygen as only ring hetero atoms containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
GBGB9913694.7A 1999-06-11 1999-06-11 DNA manipulation methods and applications for synthetic enzymes Ceased GB9913694D0 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
GBGB9913694.7A GB9913694D0 (en) 1999-06-11 1999-06-11 DNA manipulation methods and applications for synthetic enzymes
AU55457/00A AU5545700A (en) 1999-06-11 2000-06-12 Dna manipulation methods and applications for synthetic enzymes
PCT/GB2000/002286 WO2000077181A2 (en) 1999-06-11 2000-06-12 Dna manipulation methods, applications for synthetic enzymes and use for polyketide production
EP00940533A EP1190045A2 (en) 1999-06-11 2000-06-12 Dna manipulation methods , applications for synthetic enzymes and use for polyketide production
CA002376559A CA2376559A1 (en) 1999-06-11 2000-06-12 Dna manipulation methods, applications for synthetic enzymes and use for polyketide production

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9913694.7A GB9913694D0 (en) 1999-06-11 1999-06-11 DNA manipulation methods and applications for synthetic enzymes

Publications (1)

Publication Number Publication Date
GB9913694D0 true GB9913694D0 (en) 1999-08-11

Family

ID=10855226

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB9913694.7A Ceased GB9913694D0 (en) 1999-06-11 1999-06-11 DNA manipulation methods and applications for synthetic enzymes

Country Status (5)

Country Link
EP (1) EP1190045A2 (en)
AU (1) AU5545700A (en)
CA (1) CA2376559A1 (en)
GB (1) GB9913694D0 (en)
WO (1) WO2000077181A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10021267A1 (en) * 2000-04-26 2002-01-24 Actinodrug Pharmaceuticals Gmb Process for the production of modular enzyme systems by synthesis of their genes in cyclically repeatable synthesis steps
WO2010070295A1 (en) 2008-12-18 2010-06-24 Iti Scotland Limited Method for assembly of polynucleic acid sequences
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
US11643648B2 (en) 2019-04-01 2023-05-09 National University Corporation Kobe University Method for constructing chimeric plasmid library

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4963487A (en) * 1985-01-03 1990-10-16 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US4713337A (en) * 1985-01-03 1987-12-15 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
FR2738841B1 (en) * 1995-09-15 1997-11-21 Centre Nat Rech Scient PROCESS FOR THE POLYMERIZATION OF NUCLEIC ACID SEQUENCES AND ITS APPLICATIONS
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
AU694393B2 (en) * 1996-09-26 1998-07-16 National Institute Of Agrobiological Resources, Ministry Of Agriculture, Forestry And Fisheries New high capacity binary shuttle vector
AU6443298A (en) * 1997-02-28 1998-09-18 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
CA2288030A1 (en) * 1997-04-30 1998-11-05 Kosan Biosciences, Inc. Combinatorial polyketide libraries produced using a modular pks gene cluster as scaffold
GB9908814D0 (en) * 1999-04-16 1999-06-09 Celltech Therapeutics Ltd Process

Also Published As

Publication number Publication date
WO2000077181A3 (en) 2001-05-10
WO2000077181A2 (en) 2000-12-21
EP1190045A2 (en) 2002-03-27
AU5545700A (en) 2001-01-02
CA2376559A1 (en) 2000-12-21

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)