GB930428A - A method for purification of 5-purine-nucleotides - Google Patents
A method for purification of 5-purine-nucleotidesInfo
- Publication number
- GB930428A GB930428A GB2808061A GB2808061A GB930428A GB 930428 A GB930428 A GB 930428A GB 2808061 A GB2808061 A GB 2808061A GB 2808061 A GB2808061 A GB 2808061A GB 930428 A GB930428 A GB 930428A
- Authority
- GB
- United Kingdom
- Prior art keywords
- water
- methanol
- nucleotides
- purine
- volume
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
51-Purine nucleotides are separated from an admixture thereof with 51 pyrimidine nucleotides by crystallisation of the di-sodium salts from a mixture of water and methanol, ethanol, n-propanol or acetone either by adding organic solvent to an aqueous solution of the di-sodium salts or by dissolution thereof in a hot solvent mixture and cooling the solution. One part by weight of sodium salts is preferably crystallised from 4 to 10 parts by volume of water together with one third to twice the volume (relative to the volume of water) of the organic solvents; in the case of methanol the ratio of volumes of methanol to water preferred is between 0.5 to 2 : 1. The product may be recrystallised from the aqueous solvent system. The starting material may be obtained by hydrolysing nucleic acids or their partial hydrolysates with a suitable enzyme system or an organism producing such a system to yield substantialy equal amounts of 51 purine and 51 pyrimidine nucleotides. A list of suitable organisms is given. The destructive action of phosphomono esterases, if present, is inhibited by a suitable additive and 51 adenylic acid may be deaminated by an enzyme system or diazotisation. The crude hydrolysate may be further purified by contacting with active carbon and/or ion exchange resins.ALSO:51-Purine nucleotides separated from mixtures thereof with 51-pyrimidine nucleotides by crystallizing the disodium salts from a water-methanol, -ethanol, -n-propanol or -acetone mixture (see Group IV (b)) are used as condiments.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2808061A GB930428A (en) | 1961-08-02 | 1961-08-02 | A method for purification of 5-purine-nucleotides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB2808061A GB930428A (en) | 1961-08-02 | 1961-08-02 | A method for purification of 5-purine-nucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB930428A true GB930428A (en) | 1963-07-03 |
Family
ID=10269951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2808061A Expired GB930428A (en) | 1961-08-02 | 1961-08-02 | A method for purification of 5-purine-nucleotides |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB930428A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3278517A (en) * | 1963-05-15 | 1966-10-11 | Kyowa Hakko Kogyo Kk | Method for recovering nucleotide and the salts thereof from fermentation broth |
| US4151349A (en) * | 1977-02-01 | 1979-04-24 | Boehringer Mannheim Gmbh | Crystalline potassium salt of β-nicotinamide adenine dinucleotide phosphoric acid and method for its preparation |
-
1961
- 1961-08-02 GB GB2808061A patent/GB930428A/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3278517A (en) * | 1963-05-15 | 1966-10-11 | Kyowa Hakko Kogyo Kk | Method for recovering nucleotide and the salts thereof from fermentation broth |
| US4151349A (en) * | 1977-02-01 | 1979-04-24 | Boehringer Mannheim Gmbh | Crystalline potassium salt of β-nicotinamide adenine dinucleotide phosphoric acid and method for its preparation |
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