GB9106007D0 - Improvements in,or relating to,volume measurement of microbial organisms - Google Patents

Improvements in,or relating to,volume measurement of microbial organisms

Info

Publication number
GB9106007D0
GB9106007D0 GB9106007A GB9106007A GB9106007D0 GB 9106007 D0 GB9106007 D0 GB 9106007D0 GB 9106007 A GB9106007 A GB 9106007A GB 9106007 A GB9106007 A GB 9106007A GB 9106007 D0 GB9106007 D0 GB 9106007D0
Authority
GB
United Kingdom
Prior art keywords
relating
volume measurement
microbial organisms
image
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB9106007A
Other versions
GB2254414A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College London
Original Assignee
University College London
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University College London filed Critical University College London
Priority to GB9106007A priority Critical patent/GB2254414A/en
Publication of GB9106007D0 publication Critical patent/GB9106007D0/en
Priority to JP4506551A priority patent/JPH06508021A/en
Priority to PCT/GB1992/000508 priority patent/WO1992016614A1/en
Priority to EP19920907138 priority patent/EP0575450A1/en
Publication of GB2254414A publication Critical patent/GB2254414A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method and apparatus for cell volume and biomass estimation by sampling a fermentation broth and analyzing an image of the broth using brightness thresholds to distinguish between background, cellular regions and cytoplasmic and degenerated regions and summing corresponding areas in the image. Volumes can be calculated from the areas by treating the organisms as geometrical bodies and biomass derived by multiplying the volume by a density.
GB9106007A 1991-03-21 1991-03-21 Volume measurement of microbial organisms. Withdrawn GB2254414A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GB9106007A GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.
JP4506551A JPH06508021A (en) 1991-03-21 1992-03-20 Improvements in measuring biomass and differentiation in microbial organisms or related matters
PCT/GB1992/000508 WO1992016614A1 (en) 1991-03-21 1992-03-20 Improvements in, or relating to, biomass and differentiation measurements on microbial organisms
EP19920907138 EP0575450A1 (en) 1991-03-21 1992-03-20 Improvements in, or relating to, biomass and differentiation measurements on microbial organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9106007A GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.

Publications (2)

Publication Number Publication Date
GB9106007D0 true GB9106007D0 (en) 1991-05-08
GB2254414A GB2254414A (en) 1992-10-07

Family

ID=10691956

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9106007A Withdrawn GB2254414A (en) 1991-03-21 1991-03-21 Volume measurement of microbial organisms.

Country Status (4)

Country Link
EP (1) EP0575450A1 (en)
JP (1) JPH06508021A (en)
GB (1) GB2254414A (en)
WO (1) WO1992016614A1 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4095811B2 (en) * 2002-02-20 2008-06-04 正仁 田谷 Exfoliated cell sorting apparatus, exfoliated cell sorting method and program thereof
DE10347123B4 (en) 2003-10-02 2005-07-28 Petra Dr.-Ing. Perner Method for the automatic determination of airborne particles collected on a carrier, in particular fungal spores
WO2006057444A1 (en) * 2004-11-25 2006-06-01 National University Corporation Hokkaido University Method of automatically evaluating differentiation degree of cells
JP5332610B2 (en) 2006-05-22 2013-11-06 株式会社ニコン Cell detachment determination apparatus, cell detachment determination method, and cell culture apparatus
JP5239128B2 (en) * 2006-05-22 2013-07-17 株式会社ニコン Observation device
JP2011516833A (en) 2008-03-21 2011-05-26 アボット・ポイント・オブ・ケア Method and apparatus for analyzing individual cells or particulate matter using fluorescence quenching and / or fluorescence fading
US7903241B2 (en) 2008-03-21 2011-03-08 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
CA2719004C (en) 2008-03-21 2013-06-18 Abbott Point Of Care, Inc. Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample
ES2392380T3 (en) 2008-03-21 2012-12-10 Abbott Point Of Care, Inc. Procedure and apparatus for determining the hematocrit of a blood sample using the intrinsic pigmentation of the hemoglobin contained in the red blood cells
ES2560098T3 (en) 2008-03-21 2016-02-17 Abbott Point Of Care, Inc. Method and apparatus for detecting and counting platelets individually and in aggregates
CN102047118A (en) 2008-04-02 2011-05-04 艾博特健康公司 Method for serologic agglutination and other immunoassays performed in a thin film fluid sample
EP2269033B1 (en) 2008-04-09 2016-09-21 Abbott Point Of Care, Inc. Method for measuring the area of a sample disposed within an analysis chamber
ES2438841T3 (en) 2009-12-31 2014-01-20 Abbott Point Of Care, Inc. Method and apparatus for determining the average cell volume of red blood cells in the blood
US8472693B2 (en) 2010-03-18 2013-06-25 Abbott Point Of Care, Inc. Method for determining at least one hemoglobin related parameter of a whole blood sample
JP2016116461A (en) * 2014-12-19 2016-06-30 パナソニック株式会社 Cell determination method, and cell number measurement method

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1385679A (en) * 1971-04-17 1975-02-26 Image Analysing Computers Ltd Density measurement by image analysis
US4045655A (en) * 1973-10-15 1977-08-30 Hitachi, Ltd. Automatic cyto-screening device
GB1519704A (en) * 1975-04-09 1978-08-02 Zeiss Stiftung Method and means for measuring substance volumes in light-trasnmitting samples
US4453266A (en) * 1980-04-21 1984-06-05 Rush-Presbyterian-St. Luke's Medical Center Method and apparatus for measuring mean cell volume of red blood cells
DE3211314C2 (en) * 1982-03-26 1987-04-23 Vsesojuznyj naučno-issledovatel'skij institut biosinteza belkovych veščestv, Moskau/Moskva Method for determining characteristics of suspensions of n-paraffin oxidizing yeasts
JPH07114685B2 (en) * 1983-07-28 1995-12-13 株式会社日立製作所 Sewage treatment equipment
FR2555754A1 (en) * 1983-11-28 1985-05-31 Inter Inf METHOD AND DEVICE FOR AUTOMATICALLY ANALYZING BIOLOGICAL SAMPLES
DD231573A1 (en) * 1984-12-21 1986-01-02 Akad Wissenschaften Ddr METHOD AND ARRANGEMENT FOR DETERMINING PARTICLE CONCENTRATION IN LIQUIDS

Also Published As

Publication number Publication date
JPH06508021A (en) 1994-09-14
EP0575450A1 (en) 1993-12-29
WO1992016614A1 (en) 1992-10-01
GB2254414A (en) 1992-10-07

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)