GB736919A - Preservation of microbial spores or cells - Google Patents
Preservation of microbial spores or cellsInfo
- Publication number
- GB736919A GB736919A GB16967/53A GB1696753A GB736919A GB 736919 A GB736919 A GB 736919A GB 16967/53 A GB16967/53 A GB 16967/53A GB 1696753 A GB1696753 A GB 1696753A GB 736919 A GB736919 A GB 736919A
- Authority
- GB
- United Kingdom
- Prior art keywords
- crystals
- granules
- carrier
- spores
- desiccant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
An animal food supplement consists of a food supplement such as skim milk or vitamins and viable spores or cells of fungi including the subdivision Schizomycetes, and especially the bacterial flora from rumen juice, preserved by mixing a moist but not liquid host medium containing the spores or cells, with a sterile water-soluble non-toxic crystalline carrier in granule form so that the spores or cells adhere to the surfaces of the carrier granules, and dehydrating the mixture thus produced by mixing it with a sterile desiccant in granule form. It is preferred that the carrier granules be smaller in size, e.g. 50 to 200 mesh, than the desiccant granules, e.g. 5 to 50 mesh, so that, if desired, the carrier granules may be separated from the desiccant granules after the dehydration step. The preferred carrier is sucrose crystals, which are sterilized at 100 DEG to 110 DEG C. for 12 to 24 hours. Ten grams of the sterile crystals are shaken up in a Petri dish containing the mature spores of a micro-organism grown on a solid agar agar nutrient medium, and the spore-coated crystals so produced are then shaken with 10 grams of sterile desiccant granules in another Petri dish. Other carriers specified are sugars such as lactose, fructose and dextrose; sodium chloride, magnesium sulphate, sodium nitrate, agar agar granules, gelatine crystals and egg albumen crystals. Specified dehydrating agents are granules of calcium chloride, mixtures of calcium sulphate and sodium sulphate; and silica gel crystals, preferably containing an indicator which is blue when the crystals are dry and pink when wet. The spores or cells may be transferred as centrifuged sediment from a liquid medium or from any non-liquid, but moist, solid medium, and the quantities of carrier crystals and desiccant particles are chosen to provide the apparently dry, free-flowing mixture.ALSO:Viable spores or cells of Fungi including the subdivision Schizomycetes are preserved by mixing a moist but not liquid host medium containing the spores or cells, with a sterile water-soluble non-toxic crystalline carrier in granule form so that the spores or cells adhere to the surfaces of the carrier granules, and dehydrating the mixture thus produced by mixing it with a sterile desiccant in granule form. It is preferred that the carrier granules be smaller in size, e.g. 50 to 200 mesh, than the desiccant granules, e.g. 5 to 50 mesh, so that, if desired, the carrier granules may be separated from the desiccant granules after the dehydration step. The preferred carrier is sucrose crystals, which are sterilized at 100 DEG to 110 DEG C. for 12 to 24 hours. Ten grams of the sterile crystals are shaken up in a Petri dish containing the mature spores of a micro-organism grown on a solid agar agar nutrient medium, and the spore-coated crystals so produced are then shaken with 10 grams of sterile desiccant granules in another Petri dish. Other carriers specified are sugars such as lactose, fructose and dextrose; sodium chloride, magnesium sulphate, sodium nitrate, agar agar granules, gelatine crystals and egg albumen crystals. Specified dehydrating agents are granules of calcium chloride, mixtures of calcium sulphate and sodium sulphate; and silica gel crystals, preferably containing an indicator which is blue when the crystals are dry and pink when wet. The spores or cells may be transferred as centrifuged sediment from a liquid medium or from any non-liquid, but moist, solid medium, and the quantities of carrier crystals and desiccant particles are chosen to provide the apparently dry, free-flowing mixture. Specified are Penicillium notatum, Aspergillus glaucus, Saccharomyces cerevisiae, Streptomyces griseus, Bacillus subtilis, Clostridium Welchii, Escherichia coli, Lactobacillus leichmanii and Micrococcus aureus.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US736919XA | 1952-07-12 | 1952-07-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB736919A true GB736919A (en) | 1955-09-14 |
Family
ID=22115196
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB16967/53A Expired GB736919A (en) | 1952-07-12 | 1953-06-19 | Preservation of microbial spores or cells |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB736919A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012138656A1 (en) * | 2011-04-04 | 2012-10-11 | Dairy Manufacturers, Inc. | Composition and method for delivery of living cells in a dry mode having a surface layer |
AU2006260947B2 (en) * | 2005-06-23 | 2012-12-13 | Dsm Ip Assets B.V. | Process for obtaining lipid from cells |
CN103109647A (en) * | 2013-03-08 | 2013-05-22 | 北京农学院 | Method for collecting bolete spores |
US11440853B2 (en) | 2017-02-28 | 2022-09-13 | Drylet, Inc. | Systems, methods, and apparatus for increased wastewater effluent and biosolids quality |
-
1953
- 1953-06-19 GB GB16967/53A patent/GB736919A/en not_active Expired
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2006260947B2 (en) * | 2005-06-23 | 2012-12-13 | Dsm Ip Assets B.V. | Process for obtaining lipid from cells |
WO2012138656A1 (en) * | 2011-04-04 | 2012-10-11 | Dairy Manufacturers, Inc. | Composition and method for delivery of living cells in a dry mode having a surface layer |
US9296989B2 (en) | 2011-04-04 | 2016-03-29 | Drylet Llc | Composition and method for delivery of living cells in a dry mode having a surface layer |
US10047339B2 (en) | 2011-04-04 | 2018-08-14 | Drylet, Llc | Composition and method for delivery of living cells in a dry mode having a surface layer |
CN103109647A (en) * | 2013-03-08 | 2013-05-22 | 北京农学院 | Method for collecting bolete spores |
US11440853B2 (en) | 2017-02-28 | 2022-09-13 | Drylet, Inc. | Systems, methods, and apparatus for increased wastewater effluent and biosolids quality |
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