GB729208A - Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof - Google Patents

Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof

Info

Publication number
GB729208A
GB729208A GB24848/51A GB2484851A GB729208A GB 729208 A GB729208 A GB 729208A GB 24848/51 A GB24848/51 A GB 24848/51A GB 2484851 A GB2484851 A GB 2484851A GB 729208 A GB729208 A GB 729208A
Authority
GB
United Kingdom
Prior art keywords
antibiotic
solvent
ammonium
chloroform
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB24848/51A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Charles Pfizer and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc, Charles Pfizer and Co Inc filed Critical Pfizer Inc
Publication of GB729208A publication Critical patent/GB729208A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A new antibiotic named P.A. 95, effective against mycobacteria, is produced by growing in a nutrient medium a P.A. 95 producing strain of Streptomyces roseochromogenus of the type described with reference to isolate No. 7143-3, or a mutant thereof. PA95 is acidic forming metal, ammonium and organic amine salts; melting-point 138 DEG C.; empirical formula C9H15NSO3; sparingly soluble in water, soluble in methanol, acetone, chloroform and butanol; <FORM:0729208/IV (b)/1> (2 per cent aqueous sodium bicarbonate); characteristic infra-red absorption spectrum. Nutrient medium consists of carbohydrate source, e.g. starch, glycerol or sugar; organic nitrogen source, e.g. soybean meal, wheat gluten, cottonseed meal or protein hydrolysates; growth promoter, e.g. distillers' solubles, yeast extract, vitamin source; buffer, e.g. calcium carbonate; and antifoaming agent, e.g. lard oil, soybean oil or silicone. The micro-organism is grown under submerged conditions of agitation and aeration at 24 DEG to 30 DEG C., preferably 28 DEG C., for 2 to 7 days at approximately pH 7.0. The antibiotic may be isolated by (a) treating the filtered broth with activated carbon and eluting the antibiotic from the separated carbon by means of aqueous-solvent mixtures, e.g. acetone-water; (b) adsorption on strongly basic or strongly acidic ion-exchange resins, e.g. "Amberlite" (R.T.M.) I.R. 120 and I.R.A. 400, and eluting therefrom with a solution of an inorganic salt, e.g. ammonium chloride or ammonium sulphate; and (c) extracting the broth at a pH 2.0 with a water-immiscible solvent, e.g. butanol, amyl alcohol or benzyl alcohol, and thereafter extracting at a pH 9.0. After the removal of the solvent, the eluate of (a) may be further purified by method (b). The eluate of (b) may be freeze dried, or the pH adjusted to 4.5, dried and extracted with chloroform. The crystalline antibiotic may be obtained by evaporation of the chloroform extract, or the addition thereto of a non-solvent, e.g. hexane. The antibiotic may be dissolved in hot ethylene dichloride filtered through charcoal and cooled. The ammonium salt is produced by bubbling anhydrous ammonia through a solution of the acid form of P.A. 95 in ether. The sodium salt may be prepared by dissolving the antibiotic in sodium bicarbonate solution.
GB24848/51A 1951-06-15 1951-10-24 Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof Expired GB729208A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US729208XA 1951-06-15 1951-06-15

Publications (1)

Publication Number Publication Date
GB729208A true GB729208A (en) 1955-05-04

Family

ID=22110559

Family Applications (1)

Application Number Title Priority Date Filing Date
GB24848/51A Expired GB729208A (en) 1951-06-15 1951-10-24 Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof

Country Status (1)

Country Link
GB (1) GB729208A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2938836A (en) * 1954-08-19 1960-05-31 Lab Francais Chimiotherapie Process of preparing omicron-carbamyl-d-serine
WO1997033905A1 (en) * 1996-03-14 1997-09-18 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material
US6031082A (en) * 1996-03-14 2000-02-29 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2938836A (en) * 1954-08-19 1960-05-31 Lab Francais Chimiotherapie Process of preparing omicron-carbamyl-d-serine
WO1997033905A1 (en) * 1996-03-14 1997-09-18 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material
US6031082A (en) * 1996-03-14 2000-02-29 Novo Nordisk A/S Increased yields of a crystallized protein by using a solid adsorption material

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