GB697351A - Improved process for the manufacture of nerve sedatives - Google Patents

Abstract

A sedative for all the peripheral nerves is prepared by inoculating or infecting organs or tissues of animals such as cows or rabbits with cowpox, removing the organs or tissues 3 to 10 days after inoculation or infection, dispersing them in an aqueous medium, completely removing protein ingredients from the dispersion, and recovering the sedative, which contains no virus protein or lipoid, from the liquid so obtained. The aqueous medium is physiological saline solution, water, glycerin water, or a mixture thereof, containing carbolic acid. In the example, a portion of the testicles or skins of animals is removed 4-5 days after inoculation, well-ground and dispersed in a mixture of physiological saline solution, glycerin and carbolic acid. The dispersion is alternatively frozen at -5 to -10 DEG C. and thawed at 15-20 DEG C., allowed to stand, the supernatant liquid decanted and proteins removed in stages by (a) heating to 100 DEG C. for 1 hour (b) adjusting pH to 5 by the addition of hydrochloric acid and (c) filtering through asbestos treated with sodium silicate and magnesium sulphate. The filtrate from (c) is treated with kaolin at pH 4.5-4.7. The absorbate containing the virus and sedative is eluted with N/24-N/26 aqueous ammonia and filtered. The filtrate is adjusted to pH 7.4-7.8 with N/1 acetic acid, concentrated in vacuo, and the sedative precipitated by the addition of a water-miscible organic solvent e.g. acetone or ethylalcohol. The effective material can be isolated by cataphoresis of the clear liquid obtained after heating and freezing a dispersion of the tissues in saline solution. Active material may also be isolated from urine of infected animals or from a cowpox inoculated hatching egg.