GB2613660A - Laundry sanitizing and softening composition - Google Patents

Abstract

A laundry composition comprising a cationic biocidal active substance, a cationic softener and an unsaturated fatty alcohol emulsifier. The emulsifier may be a mono-unsaturated C8-C26 fatty alcohol and may comprise oleyl, palmitoleyl or erucyl alcohol. The biocidal active substance may be selected from dialkyl dimethyl ammonium chloride (DDAC), alkyl dimethyl benzyl ammonium chloride (ADBAC), alkyl ethylbenzyl ammonium chloride, (ADEBAC, EDC) or combinations thereof. The cationic softener may be an esterquat. The composition may comprise 1 to 5 wt. % of the cationic active substance, 1 to 25 wt. % of the cationic softener and 0.5 to 4 wt. % of the emulsifier. The weight ratio of biocide to softener to emulsifier may be 1-40:1-40:1. The composition may further comprise a solvent or non-ionic surfactant.

Description

LAUNDRY SANITIZING AND SOFTENING COMPOSITION

The present invention relates to a laundry sanitizing and softening composition to be added to a laundry washing process to provide improved reduction in bacteria and viruses during a laundry washing process while also providing fabric softening.

Background

Consumers have increased interest in washing laundry under energy and water efficient conditions due to environmental concerns, and also washing with laundry compositions with reduced environmental impact, such as less environmentally harmful laundry detergents. Consumers are therefore interested in washing laundry at cooler temperatures, with less water and with milder laundry detergents. Washing laundry under these conditions raises concerns that the resulting items may be visibly clean but may be unhygienic because they may still harbour pathogens. Therefore, there is a growing demand for laundry sanitizing compositions to ensure that clean laundry harbours fewer pathogens. However, laundry sanitizing compositions are added to the fabric softener drawer of the washing machine, making it difficult to use both laundry sanitizing compositions and fabric softener compositions in the same wash cycle.

Liquid fabric softener (also known as fabric conditioner) compositions are typically a dispersion of cationic quaternary ammonium surfactants in an aqueous carrier. These cationic quaternaly ammonium surfactants form positively charged vesicles dispersed in the carrier. Adding further cations to the system may affect the stability of the vesicles, leading to flocculation. Thus, it has been difficult to combine in a single composition the actives used in laundry sanitizing compositions, typically cationic biocidal agents, with the cationic quaternary ammonium surfactants used as fabric softening agents in liquid fabric softeners.

WO 96/21715 seeks to provide a means for stabilizing dispersions of cationic fabric softeners in liquid carriers, particularly those containing added electrolytes, by using certain polymers incorporated into concentrated fabric softener compositions to -1 -enhance stability. In particular, when formulating fabric softeners with higher concentrations of cationic surfactant it is usually desirable to incorporate materials such as inorganic electrolytes, e.g. to de-water the softener vesicles and to incorporate perfume with the cationic vesicles to stabilise the perfume. It is suggested that the polymers interact with the cationic softener vesicles, inhibiting their coalescence in the presence of high ionic strength, such as when adding further cations to the system Suggested polymers are water-soluble polyesters that can be formed from: (1) ethylene glycol, 1,2-propylene glycol or a mixture thereof; (2) a polyethylene glycol (PEG) capped at one end with a C1-4 alkyl group; and (3) a dicarboxylic acid (or its diester).

US 3,349,033 relates to microbiologically active softeners. It discusses how mixtures of germicidal quaternary ammonium salts with fabric softeners are unstable, remaining homogeneous for at most a few weeks. This instability is allegedly overcome by adding a small amount of an emulsifier (e.g. a long chain fatty alcohol) to the mixture causing the mixture to remain homogenously emulsified for longer and under successive freeze-thaw cycles. The long chain fatty alcohol emulsifiers are disclosed to be C10-18 fatty alcohols, such as "topped fatty alcohols" like "Lorol 5", disclosed to have an alkyl chain distribution of 3% Cm, 61% Cu, 23% C14, 11% C16 and 2% C18. The cationic quaternary ammonium softeners discussed are of the formula NR'R"R"R-X, where R and R' are C10-20 alkyl groups; R" is methyl; R" is methyl, ethyl or (CH2-CH0)H where n>0, and X is chlorine, bromine, sulfate, methosulfate or ethosulfate. An example of a common contemporaneous cationic quaternary ammonium softener is di-(hydrogenated tallow) dimethyl ammonium methosulfate, which has since been superseded by more biodegradable compounds, such as esterquats. The germicidal quaternary ammonium salts used in US 3,349,033 are of the single-chain variety, such as Alkyl Dimethyl Benzyl Ammonium Chloride ("ADBAC" or "BKC"). Germicidal quaternary ammonium salts have developed considerably since US 3,349,033 with Alkyl Dimethyl EthylBenzyl Ammonium Chloride ("ADEBAC" or "EBC") and Dialkyl Dimethyl Ammonium Chloride ("DDAC", a twin-chain quat) becoming available, as well as mixtures of these quaternary ammonium salts. DDAC is the most efficacious germicidal quaternary ammonium and has improved tolerance for anionic surfactants, organic loads and hard water compared with the single-chain quats BKC and EBC. EBC -2 -is typically used in combination with BKC. Both the germicidal quaternary ammonium salts and the fabric softener actives have therefore advanced considerably since US 3,349,033.

W02002/072745A I provides a rinse added composition for the conditioning of fabric in a rinse, the composition comprising a fabric softener active, a suds suppressing system and a surfactant scavenger.

W02006/094582A1 discusses how consumers prefer liquid fabric conditioners that appear thick and creamy, which is indicated by having a high viscosity and a high opacity. Thin, translucent, or watery liquid fabric conditioners are perceived as cheap and/or ineffective. W02006/094582A1 suggests that polymeric viscosity modifiers may be used to impart a high viscosity and opacity to the composition and uses water-soluble polysaccharide polymers to this effect.

W097/47716A2 describes aqueous softening dispersions containing 0.5 to 20 wt% of at least one non-ionic fat, 0.2 to 10 wt % of a water-soluble or insoluble cationic emulsifier and possibly up to 10 wt% of a non-ionic emulsifier, in which the weight ratios between fat and emulsifiers is between 10:1 and 0.5:1, and which are alleged to exhibit a good soft-care effect if the zeta potential of the dispersion is at least +30 niV at 25°C and a pH of 7.

GB2207446 relates to aqueous fabric softening compositions based on cationic fabric softeners and fatty alcohols. The weight ratio of cationic softening agent to fatty alcohols is in the range of from about 6:1 to 2,8:1.

W002120706A1 describes a method of preparing an aqueous dispersion of a fabric conditioning composition comprises mixing 7.5 to 60 wt% of a cationic fabric softening compound with 0.01 % -1.5 wt% of a non-ionic surfactant viscosity modifier and up to 1.4 % by weight of a fatty alcohol, the combination of (b) and (c) being no more than 1.5 % by weight, based on the total weight of the composition. Saturated fatty alcohols are preferred as these were considered to deliver greater benefits in terms of stability, especially low temperature stability.

W003/022972A I also describes a method of preparing an aqueous, liquid fabric conditioning composition wherein saturated fatty alcohols are preferred as these were -3 -considered to deliver greater benefits in terms of stability, especially low temperature stability.

Dettol® Laundry Cleanser is one commercially available product that is used for sanitizing clean laundry. It contains the dialkyl (C8-11)) dimethylammonium chloride (DDAC) and benzyl-C12-1s-alkyldimethyl chloride (ADBAC). Typically, about I 20m1 of Dettol® Laundry Cleanser is added to the fabric softener drawer of the washing machine so that it is used in the washing machine rinse cycle. Dettol® Laundry Cleanser kills 99.9% of bacteria (i.e. a 3-log reduction in bacteria) in laboratory tests.

There remains a need for laundry softener compositions that also provide germ kill.

It is an object of the invention to provide a laundry softener composition that also provides germ kill during a laundry rinse cycle.

Statement of invention

In a first aspect, the invention provides a laundry composition comprising: a) at least one cationic biocidal active; b) at least one cationic fabric softener, and c) at least one emulsifier, wherein the at least one emulsifier is an unsaturated fatty alcohol.

In a further aspect, the invention provides the use of an unsaturated fatty alcohol (such as those described herein) for stabilising a cationic liquid dispersion containing at least one cationic surfactant. Preferably, the use of an unsaturated fatty alcohol (such as those described herein) for preventing flocculation in a cationic liquid dispersion containing at least one cationic surfactant. The at least one cationic surfactant may be a cationic biocide (such as a cationic quaternary ammonium surfactant, e.g. those described herein), a fabric softener (such as those described herein), and a combination of a cationic biocide and a fabric softener. The carrier liquid may be aqueous. The liquid dispersion may be a dispersion of vesicles.

In yet a further aspect, the invention provides a method of stabilising a cationic liquid dispersion containing at least one cationic surfactant, comprising adding an unsaturated fatty alcohol emulsifier (such as those described herein) to a liquid dispersion containing the at least one cationic surfactant. The unsaturated fatty alcohol prevents -4 -flocculation in the liquid dispersion, thus stabilising the cationic liquid dispersion. The cationic surfactant may be a cationic biocidal active (such as a cationic quaternary ammonium surfactant, e.g. those described herein), a cationic fabric softener (such as those described herein), and a combination of a cationic biocidal active and a cationic fabric softener. The carrier liquid may be aqueous. The liquid dispersion may be a dispersion of vesicles.

Cationic Fabric Softener Preferably, the at least one fabric softener is a cationic quaternary ammonium surfactant containing ester linkages, commonly called an esterquat. More preferably, the at least one fabric softener is a cationic quaternary ammonium surfactant derivable from the reaction of triethanolamine with fatty alcohols. Yet more preferably, the fatty alcohols are partially hydrogenated. The fatty alcohols may be derived from plant oils (e.g. palm, palm kernel or coconut oil) or animal sources (e.g. tallow). The cationic fabric softener may be a partially hydrogenated palm esterquat (such as Tetranyll) L6/90 available from Kao) or a partially hydrogenated tallow esterquat (e.g. dihydrogenerated tallowethyl, ditallowethyl hydroxyethylmonium methosulfate, such as Tetranyl® AT7590 available from Kao)).

Emulsifier The at least one emulsifier is an unsaturated fatty alcohol. Preferably, the at least one unsaturated fatty alcohol contains at least one cis double bond. More preferably, the at least one unsaturated fatty alcohol is a monounsaturated fatty alcohol. The at least one unsaturated fatty alcohol may be a C8-26 unsaturated fatty alcohol, or a C10-26 unsaturated fatty alcohol. Preferably, the at least one unsaturated fatty alcohol is a Cp_26 unsaturated fatty alcohol, more preferably the at least one unsaturated fatty alcohol is a C16-22 unsaturated fatty alcohol. The unsaturated fatty alcohol may be oleyl alcohol (cis-9-octadecen-1 -ol); palmitoleyl alcohol (cis-9-hexadecen-1 -ol), erucyl alcohol (cis-13-docosen-1 -ol), preferably oleyl alcohol (cis-9-octadecen-1-01).

Cationic biocidal active The at least one cationic biocidal active may be selected from one or more quaternary ammonium compounds. Quaternary ammonium compounds are compounds -5 -of the formula (WRIR2R3R4)X-, where X-is a salt-forming anion which permits water solubility of the quaternary ammonium compounds, and is, for example, chloride, bromide or iodide or a methosulfate anion. Any quaternary ammonium compound with antimicrobial activity may be used in the invention.

The at least one cationic biocidal active agent may be selected from one or more dialkyldimethyl ammonium chlorides (DDAC), where each alkyl group is independently selected from straight chain Cx-C10 alkyl.

The at least one cationic biocidal active agent may be selected from one or more alkyl dimethyl benzyl ammonium chlorides (ADBAC also known as BKC): H3C CH3 CI° 10, where n = 8-18, preferably n = 12-14 as these chain lengths are the most biocidal.

The at least one cationic biocidal active agent may be selected from one or more alkyl dimethyl ethylbenzyl ammonium chlorides (ADEBAC also known as EBC), which are preferably C8-12-alkyl [(ethylphenyl) methyl] dimethyl chlorides.

Preferably, the at least one cationic biocidal active agent is selected from ADBAC, ADEBAC, DDAC and combinations thereof, such as ADBAC and DDAC, or ADBAC and ADEBAC, or ADBAC, ADEBAC and DDAC. More preferably, the at least one cationic biocidal active agent is BKC or DDAC or a combination of ADBAC and DDAC. Most preferably, the at least one cationic biocidal active agent is a mixture of ADBAC and DDAC.

The at least one cationic biocidal active agent and the cationic fabric softener are different.

Additional components The laundry composition may contain chelating agents. In particular, the laundry composition may contain aminopolycarboxylates (such as EDTA); nitrilotriacetic acid (NTA); iminodisuccinic acid (IDS); polyaspartic acid; S,S-ethylenediamine-N,N'-disuccinic acid (EDDS); methylglycinediacetic acid (MGDA); and L-Glutamic acid N,Ndiacetic acid, tetrasodium salt (GLDA).

The laundry composition may contain organic acids and/or salts thereof. In particular, the laundry composition may contain organic acids that have antimicrobial -6 -activity, such as glycolic acid, citric acid, formic acid, sorbic acid, lactic acid, acetic acid, propionic acid, malic acid and mixtures thereof. Preferably, the organic acid is glycolic acid or a salt thereof.

The laundry composition may contain antifoam agents. In particular, the laundry composition may contain silicone antifoam emulsions such as those available from Wacker as SILFOAMV.

The laundry composition may contain pH adjusting agents In particular, the laundry composition may contain sulfuric acid Weights The at least one cationic biocidal active may be present in the laundry composition at about 1 to about 5 wt% of the composition. Preferably, the at least one cationic biocidal active is present at about 2 to about 4 wt% of the laundry composition. More preferably, the laundry composition comprises about 2.4 wt% or about 3.36wt% of the at least one cationic biocidal active.

The at least one cationic fabric softener may be present in the laundry composition at about 1 to about 25 wt% of the composition. Preferably, the at least one cationic fabric softener is present at about 1 to about 10 wt% of the laundry composition, more preferably at about Ito about 5wt%, yet more preferably at about 2 to about 4 wt% of the laundry composition. More preferably, the laundry composition comprises about 2.7wt% of the at least one cationic fabric softeners. Alternatively, the at least one cationic fabric softener may preferably be present in the laundry composition at about 3 to about 15 wt% of the laundry composition, more preferably at about 3 to about 10wt%. The at least one emulsifier may be present in the laundry composition at about 0.5 to about 4 wt% of the composition. Preferably, the at least one emulsifier is present at about 1 to about 3 wt% of the laundry composition. More preferably, the laundry composition comprises about 2wt% of the at least one emulsifier.

The weight ratio of the at least one cationic biocidal active: at least one cationic fabric softener: at least one emulsifier may be 1-40: 1-40: 1, preferably, about 1.2:1.35:1.

In one embodiment, the laundry composition comprises: about 1.44wt% or about 2.4wt% of at least one dialkyldimethyl ammonium chloride (DDAC), where each alkyl group is independently selected from straight chain Cs-Cio alkyl; ii. about 0.96wt% of one or more alkyl dimethyl benzyl ammonium chloride: CD,--CnE12ntl Cis H3C CH3 n=8,10,12:14,16,18 about 2.70wt% of cationic fabric softeners, and iv. about 2.00wt% oleyl alcohol.

The laundry composition may further comprise: v. about 1 wt% solvent, such as propylene glycol, and vi. about lwt% non-ionic surfactant, such as alcohol ethoxylates.

The laundry composition may optionally further comprise a pH adjuster, such as sulphuric acid.

In one embodiment, from about 30 to about 180 ml, preferably about 120 ml, of the laundry composition of the invention at about 1:159 dilution with water achieves at least a 4-log reduction according to the EN1276:2009 protocol, under clean conditions in no more than 22 minutes at 20°C. Preferably, from about 30 to about 180 ml, preferably about 120 ml, of the laundry composition of the invention at about 1:159 dilution with water achieves at least a Slog reduction according to the EN1276:2009 protocol, under clean conditions in no more than 22 minutes at 20°C.

In a second aspect:, the invention provides a method of sanitizing laundry during a laundry washing process comprising adding from about 30 to about 180 ml, preferably about 120 ml, of the laundry composition described herein to the fabric softener compartment of an automatic washing machine and using the automatic washing machine to perform a laundry washing process.

In a third aspect, the invention provides the use of an unsaturated fatty alcohol emulsifier as described herein in stabilising a laundry composition comprising at least one cationic biocidal active and a cationic fabric softener. -8 -

The inventors have surprisingly found that the inc 1 unsaturated fatty alcohol emulsifier in a laundry composition comprising a cationic fabric softener and a cauoncbiocidal agent produced gable laundry fabric softeners compositions that provide germ kill during, a laundry process.

Tr: particular, the inventors have surprisingly found that an unsaturated rafty alcohol improves the stability of a laundry composition comprising a cationic fabric softener and a cationic biocidal agent. Indeed, the laundry composition of the invention is surprisingly stable at 60°C, which is particularly unusual for fabric softener.

Terms and Definitions As used herein: the terms "approximately" and "about" mean plus or minus 10% of the value stated; the term "a" or "an" means one or more; any and all ranges are inclusive of their endpoints, e.g. a range of from lwt% to lOwt% w/w includes lwt% and lOwt% and any concentration between lwt% and lOwt%; all amounts given in % are % by weight (wt%) unless otherwise stated; the term "benzyl group" is C6H5CH2-; the term "comprising" means "includes" and encompasses the terms "consisting of' and "consisting essentially of': i.e. the compositions of the invention comprising the components as herein defined may consist essentially of these components or may consist of these components.

Examples

The following examples illustrate exemplary formulations as well as preferred embodiments of the invention. It is to be understood that these examples are provided by way of illustration only and that further useful formulations falling within the scope of the present invention and the claims may be readily produced by one skilled in the art without deviating from the scope and spirit of the invention.

Samples In the foilc.wng examples the biocidal actives used were:MAC. and BKC. These biocides are typically provided as solutions containing DDAC or RIKti and solvents, typically selected from water, ethanol, isopropyl alcohol, ethylene glycol and mixtures thereof In the following examples the DDAC used was Acticide® DIDQ80 (Thor) or -9 -Bardac® 2080 (Lonza), both are twin chain dimethyl ammonium chloride compositions comprising 80% actives with the balance comprising water and ethanol. In the following examples the BKC used was Acticide® BAC 80 (Thor) or BTC 1218-80 (Stepan), which comprise 80% actives (alkyl (CI 2-18) dimethylbenzyl ammonium chloride) with the balance comprising water and ethanol.

In the following examples the fabric softening active used was "letranyl® AT-7590 (Kau), which contained 90% active matter (partially hydrogenated tallow esterquat) and 10% isopropyl alcohol (IPA).

In the following examples the surfactant was Genapol® LA 070 (Alcohol 10 ethoxylate C12-16, 7E0, Clariant) and the solvent was propylene glycol (DOW).

Sample Preparation The following formulations were made by adding the raw materials step wise with mixing, waiting until the mixture was homogenous after each material was added before adding the next raw material. The raw materials were added in the following order.

1. Deionised water 2. Emulsifier (if used) 3. Solvent 4. Surfactant 5. DDAC 6. BKC 7. Esterquat 8. Deionised water The final step of deionised water was added in an amount sufficient to achieve the desired amount of active, e.g 1.8wt% DDAC, 1.2wt% BKC and 3wt% esterquat and included a fragrance, if used.

-10 -Ingredient A B C D Deionized Water 92.00 90.00 90.00 90.00 Propylene Glycol 1.00 1.00 1.00 1.00 Genapol ® LA 070 1.00 1.00 1.00 1.00 DDAC 1.80 1.80 1.80 1.80 BKC 1.20 1.20 1.20 1.20 Esterquat 3.00 3.00 3.00 3.00 Emulsifier Cetyl alcohol n/a. 2.00 Stearyl alcohol 2.00 Lauryl alcohol 2.00 Ingredient 1 2 3 4 Deionized Water 89.75 90.00 90.00 90.00 Propylene Glycol 1.00 2.00 1.00 1.00 Genapol® LA 070 1.00 1.00 1.00 1.00 DDAC 1.80 1.80 1.80 1.80 BKC 1.20 1.20 1.20 1.20 Esterquat 3.00 3.00 3.00 3.00 Emulsifier Oleyl alcohol 2.00 2.00 Cis-9-hexadecenol 2.00 Cis-13-docosenol 2.00 Fragrance 0.25 Stability Tests The above samples were tested for stability. The stability was quantified by 5 measuring the zeta potential of the sample using a Malvern Zetasizer Nano Series ZEN 3600.

Sample Emulsifier Stability Zeta potential at 25 °C / mV A None Unstable even at RT 55.5 B Cetyl alcohol Unstable, very dependent on process 56.6 C Stearyl alcohol Unstable even at RT 56.6 D Lauryl alcohol Stable but translucent 72.7 2 Oleyl alcohol Stable over 1 year at all conditions 72.0 3 cis-9-hexadecen-1-ol Stable over 26 weeks at all conditions 73.0 4 cis-I 3-docosen-I -ol Stable over 26 weeks at all conditions 71.3 Results inple A containing no emulsifier was unstable even at room temperature The samples containing the saturated emulsifiers cen,71 alcohol (Sample B) and stearyl alcohol (Sample C) were unstable, even at room temperature, and had comparable zeta potential values to the sample without an emulsifier, Sample A. Sample D containing the short-chain saturated emulsifier lauryl alcohol was stable but was translucent; consumers prefer opaque laundry softeners and so Sample D was not considered commercially viable. The samples containing unsaturated emulsifiers. Samples 2-4, were found to be stable over prolonged periods of time and at all conditions. A zeta potential of greater than 60 mV was considered necessary.

Two samples were prepared usimg a different order of mixing to those above. Both samples contained 4% biocide:, rather than the 3% used above. Both samples contained oley1 alcohol as the emulsifier One sample used the same esterquat as the samples above, i.e. Tetranyl© AT7590. The other sample used a partially hydrogenated palm esterquat, Tetranyl®L6/90 (Kao), which was similar to the Tetranyl® AT7590 and contained 90 % active matter and 10% [PA. Both samples were stable over 12 weeks at -12 -all conditions. The tallow-based esterquat had a zeta potential of 716 mV at 25 'V and the palm-based esterquat had a zeta potential of 74.6 triV at 25 ct Thus,, the samples containing unsaturated emulp,ifiers were mind to be stable over prolonged periods of time and at all conditions even using a different method of manufacture.

Antimicrobial Efficacy (British Standard) Two samples according to the invention (Sample 5 and Sample 6) were evaluated for their antimicrobial efficacy against Staphylococcus auretts (ATCC 6538), Escherichia co/i (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442) and Enterococcus hirae (ATCC 10541) using an antimicrobial suspension test based on British Standard reference No: EN 1276:2009 method entitled "Chemical Disinfectants and Antiseptics -Quantitative Suspension Test for the Evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -Test method and requirements (Phase 2, step 1)". A greater than 5-log reduction of all organisms (Pseudonionas aeruginosa"Staphylococcus mucus, Enterococcus hirae, Escherichia colt) for a contact time of I to 60 minutes (determined by product specification) at a testing temperature ranging from 4°C to 60°C+1.0°C (determined by product specification) is required to pass the EN1276. Additional and optional contact times, temperatures, and test organisms can be used.

The test protocol tests the efficacy of the compositions of the present invention and the comparative examples against gram-negative and gram-positive bacteria. The test organisms used were Staphylococcus (litmus (ATCC 6538), Escherichia colt (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442) and Enterococcus hirae (ATCC 10541).

The bacterial strains were cultured on tryptic soy agar (TSA) slant from frozen stock and incubated for 18-24 hours. Following incubation, 21 and 3' generation transfers were prepared and used to prepare test suspensions as described in the BS EN 1276:2009 test method. The working culture must be either a second or third generation subculture on TSA slants from a cryogenic stock. The cell suspensions were adjusted to produce approximately 1.5 -5.0 x 108 CFU mL1. Subcultures were prepared on TSA -13 -slants and incubated at 36+1°C for 18-24 hours. The growth medium and temperature used were TSA and 361°C. Test solutions and test cultures are equilibrated to a test temperature of 20±1°C in a water bath.

The tests were performed using "clean experimental conditions, i.e. 0.3g/I Bovine Albumin (BA) in-test concentration, rather than "dirty" experimental conditions, i.e. 3g/1 BA in-test concentration. For laundry sanitizers/softeners, the desired experimental conditions are "clean conditions" because the laundry sanitizer/softener is used after a laundry detergent in a laundry washing process, i.e. on clean laundry. Experimentally, a 1:1 dilution of the test culture and test conditions was 10 performed and allowed to react for 2 minutes. 2.0mL of the test culture was exposed to 8.0mL of the test product for a 22-minute contact time and then a 1.0mL aliquot was neutralized in a verified neutralizer. After a 5-minute neutralization time, the neutralized sample was serially diluted, plated on TSA and incubated at 36+1°C for 48 hours. The average Logi() CFUlnaL for the test suspension was calculated and used to compute the log reduction post-treatment. At least a 5-log reduction of all organisms tested for a 22-minute contact time was chosen to indicate that the tested formulation has the desired level of antimicrobial properties against tested organisms. Log reduction greater than 4 means >99.99% reduction in the number of cells; log reduction greater than 5 means >99.999% reduction in the number of cells and denotes complete kill.

Sample 5 was prepared as follows.

I. 439.52g of soft water was added to the beaker and heated to 40°C.

2. The following were then added while constantly mixing in between each addition: 0.03g of Silfoam® SE 39 (antifoam agent, Wacker), - 2.50g of ROKAnol® L7A (C12-16 Laureth-7, PCC), 5.00g propylene glycol, 10.00g of oleyl alcohol, - I 2.75g of DDAC (Arquad0 I -80, Nouryon) and 5.40g of BKC (Coco(fractionated)benzyldimethylammonium chloride, Arquad® MCB-80, Nouryon).

-14 - 3. The beaker was heated to 45°C.

4. 15.00g of Tetranyl® AT7590 was pre-melted at 50°C and added to the beaker once the temperature was at 50°C.

5. The mixing speed was increased as the viscosity built and mixing continued for ID minutes.

6. the following were then added while mixing: 2.00g of dye, 2.80g of fragrance and 5.00g of soft water.

7. The batch was cooled before pouring into a container for storage. The final amounts are given in the table below.

Ingredient Function % wiw Soft Water Solvent 88.904% Silfoam® SE 39 A ntifoam 0.006% ROKAno10 L7A Surfactant 0.50% Propylene glycol Solvent 1.00% Oleyl alcohol Emulsifier 2.00% Arquad® 2.1-80 Active 2.550% Arquad® MCB-80 Active I.080% Tetranyl® AT7590 Softener 3.00% Dye 0.40% Fragrance 0.56% Total: 100. 00% Sample 6 contained 3% Tetranyl® L6/90 in place of the Tetranyl® AT7590 used in Sample 5 and 0.96% BKC and 2.4% DDAC, rather than the 2.550% BKC and 1.080%, DDAC used in Sample 5.

I 5 The results from the antimicrobial efficacy tests are given in the table below.

-15 -Sample Dilution Contact time Log reduction E. coli S. aureus E hirae P aeruginosa 1:159 22min >5.09 >5.65 >5.41 >5.28 6 1:159 22min >5.07 >5.09 >5.10 >5.03 Antimicrobial Efficacy (China Technical Standard) Sample 1 above was evaluated for its antimicrobial efficacy against Staphylococcus aureus, Escherichia coil, and Candida albicans according to China Technical Standard TSD 2.1.1.9/2.1.1.7. The results from the antimicrobial efficacy tests are given in the table below.

Dilution Contact time Log reduction E. coil S. aureus C albicans >5.45 min - - 5.04 5.11 1:50 >6.66 >6.62 min >6.68 >6.57 >6.70 >6.56 >6.66 >6.62 I: 125 5 min >6.68 >6.57 >6.70 >6.56 >6.66 6.62 1:416 5 min >6.68 6.57 ->6.70 6.56 Fabric Softening, Two fabric softeners according to the invention were tested against existing laundry products, including two laundry softeners, a laundry detergent and a laundry -16 -sanitizer. The test was performed in accordance with ASTM D5237 -14 "Standard Guide for Evaluating Fabric Softeners (modified)".

Method A fabric bundle consisting of poly/cotton, cotton, and cotton terry looped towels, 5 was washed with AATCC standard detergent and rinsed using the samples. After wash/rinse completion, towels were removed and conditioned overnight at 23°C, 50% Relative Humidity (RH). Substrates were then prepared for panel evaluation.

individual panellists evaluated the blinded samples based on a Ranking Comparison. Prior to evaluations, each panellist was instructed to wash and dry hands. If re-evaluations were needed, panellists were instructed to evaluate different sections of the towel. Panellists were presented with a group of six test fabrics on a table, with each fabric presented randomly and containing a different softener treatment and a blinded control. Each of the substrates were ranked from least to most soft, with no ties allowed, using a rating scale ranging from 1-6, with 1 being the least soft and 6 being the softest.

The evaluations were all completed within the span of 2 hours. Fisher's LSD for ranked sums was performed to determine statistically significant differences, if any, between all products. Based on Fisher's LSD, any two samples can be described as significantly different if their ranked sums differ by more than the value calculated in LSDrank. All data and results can be found below.

Samples:

Sample Description

7 composition containing BKC, DDAC and Tetranyle AT7590 8 composition containing: BKC, DDAC and Tetrany10 L6/90 E commercially available fabric softener containing: 5-15% cationic surfactants; <5% perfume; DMDM hydantoin, and potassium sorbate F commercially available laundry detergent containing: alcohol ethoxylate 7E0; sodium laureth sulfate; ethanol; sodium citrate; sodium alkylbenzene sulfonate; protease; propylene glycol; sodium cocoate; sodium iminodisuccinate; triethanolamine; disodium distrylbiphenyl dissulfonate; sodium polyacrylate; calcium chloride; bronopol; sodium nitrate; 5-chloro-2-methyl-4-isothiazolin-3 -one, and 2-methy1-4- -17 -isothiazolin-3-one G commercially available laundry sanitizer containing: BKC and DDAC H commercially available fabric softener containing: 5-15% cationic surfactants; <5% perfume; alpha-isomethylionone; citronellol; eugenol; hexyl cinnamal; limonene; DMDM hydantoin, and potassium sorbate Results Subject 7 8 E F G 11 1 5 4 3 2 1 6 2 5 2 4 3 6 1 3 2 5 3 I 6 4 4 5 2 6 1 3 4 3 2 5 1 1 6 6 6 2 I 3 5 4 7 2 5 3 1 4 6 8 1 3 4 5 2 6 9 6 4 3 1 2 5 6 4 5 1 2 3 11 4 5 6 1 2 3 12 3 4 6 I 5 2 13 2 3 4 6 5 1 14 5 1 6 2 4 3 5 6 4 2 3 1 16 3 5 2 1 4 6 17 6 4 I 2 3 5 18 6 2 4 1 3 5 19 5 I 6 2 3 4 6 5 3 2 1 4 Average 4.3 3.45 3.95 1.95 3.25 3.95 Sum 86 69 79 39 65 79 -18 -Results At a significance level of a=0.05, the LSDrank was determined to be 23.19. All five Samples performed significantly better in softness than Sample F (control). Samples 7, 8, E and H, the softener samples, performed better than the sanitizer Sample F. Sample 7, a softener according to the invention, outperformed the other samples. Samples 7, 8, E, G and H did not perform significantly better than each other.

Conclusion

Thus, the laundry compositions comprising cationic b ocidal actives, cationic fabric softeners, and an unsaturated fatty alcohol emulsifier were surprisingly found to be stable over prolonged periods of time and at all conditions even using a different method of manufacture. These compositions provided a 5-log reduction in the number of bacteria cells, i.e. killed 99.999% of bacteria, while also providing a fabric softening effect comparable to existing commercially available fabric softeners.

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Claims (15)

1. Claims 1 2. 4.A laundry composition comprising: a) at least one cationic biocidal active; b) at least one cationic fabric softener, and c) at least one emulsifier, wherein the at least one emulsifier is an unsaturated fatty alcohol.
2. A laundry composition according to claim 1, wherein the at least one emulsifier is an unsaturated fatty alcohol containing at least one cis double bond, or wherein the at least one emulsifier is a monounsaturated fatty alcohol.
3. A laundry composition according to claim 1 or claim 2, wherein the at least one emulsifier is a Cg-26 unsaturated fatty alcohol, or a C10-26 unsaturated fatty alcohol, preferably the at least one unsaturated fatty alcohol is a C12-26 unsaturated fatty alcohol, more preferably a C16_7/ unsaturated fatty alcohol, for example: oleyl alcohol (cis-9-octadecen-l-ol); palm itoleyl alcohol (cis-9-hexadecen-l-ol), erucyl alcohol (cis-13-docosen-l-ol), particularly oleyl alcohol (cis-9-octadecen-1-01).
4. A laundry composition according to any preceding claim, wherein the at least one cationic biocidal active is selected from one or more quaternary ammonium compounds, for example, wherein the at least one cationic biocidal active agent is selected from: - one or more dialkyldimethyl ammonium chloride (DDAC), where each alkyl group is independently selected from straight chain Cx-Cio alkyl; - one or more alkyl dimethyl benzyl ammonium chloride (ADBAC also N CIE) HaC CHa where n = 8-18, preferably n = known as BKC) 12-14; -20 -6. 7. 8.one or more alkyl dimethyl ethylbenzyl ammonium chloride (ADEBAC also known as EBC), which are preferably C8_12-alkyl [(ethylphenyl) methyl] dimethyl chlorides; combinations of ADBAC, ADEBAC, DDAC, such as ADBAC and DDAC, or ADBAC and ADEBAC, or ADBAC, ADEBAC and DDAC.
5. A laundry composition according to any preceding claim, wherein the at least one cationic biocidal active is BKC or DDAC or a combination of ADBAC and DDAC, preferably a combination of ADBAC and DDAC.
6. A laundry composition according to any preceding claim, wherein the at least one cationic fabric softener is a cationic quaternary ammonium surfactant containing ester linkages (esterquat), preferably, the at least one fabric softener is a cationic quaternary ammonium surfactant derivable from the reaction of triethanolamine with fatty alcohols, more preferably, the fatty alcohols are partially hydrogenated, for instance the cationic fabric softener may be a partially hydrogenated palm esterquat or a partially hydrogenated tallow esterquat (e.g. dihydrogenerated tallowethyl, ditallowethyl hydroxyethylmonium methosulfate).
7. A laundry composition according to any preceding claim, wherein the laundry composition comprises: - about 1 to about 5 wt% of the at least one cationic bioc dal active, preferably about 2 to about 4 wt%; - about 1 to about 25 wt% of the at least one cationic fabric softeners, preferably about 1 to about 10 wt%, more preferably about 1 to about 5 wt%, yet more preferably about 2 to about 4 wt%, and about 0.5 to about 4 wt% of the at least one emulsifier, preferably about I to about 3 wt%.
8. A laundry composition according to any preceding claim, wherein the laundry composition comprises: -21 -about 2.4 wt% or about 3.36wt% of the at least one cationic biocidal active; -about 2.7wt% of the at least one cationic fabric softeners, and about 2wt% of the at least one emulsifier.
9. 9. A laundry composition according to any preceding claim, wherein the weight ratio of the at least one cationic biocidal active: at least one cationic fabric softeners: at least one emulsifier is 1-40: 1-40: 1.
10. 10. A laundry composition according to any preceding claim, wherein the weight ratio of the at least one cationic biocidal active: at least one cationic fabric softeners: at least one emulsifier is about 1.2:1.35:1.
11. 11 A laundry composition according to any preceding claim, wherein the laundry composition comprises: i. about 1.44wt9/o or about 2.8wt% of at least one dialkyldimethyl ammonium chloride (DDAC), where each alkyl group is independently selected from straight chain C8-Cio alkyl; ii. about 0.96wt% of one or more alkyl dimethyl benzyl ammonium chloride: Hac CfrisCi n = 8; 10, 12, 14, 16, 18 about 2.70wt% of cationic fabric softeners, and iv. about 2.00wt% oleyl alcohol.
12. 12. A laundry composition according to claim 11, wherein the laundry composition further comprises: v. about I wt% solvent, such as propylene glycol, and vi. about I wt% non-ionic surfactant, such as alcohol ethoxylates.
13. -22 - 13 A method of sanitizing and softening laundry during a laundry washing process comprising adding from about 30 to about 180 ml, preferably about 120 ml, of the laundry composition according to any preceding claim to the fabric softener compartment of an automatic washing machine and using the automatic washing machine to perform a laundry washing process.
14. 14. Use of an unsaturated fatty alcohol emulsifier in stabilising a laundry composition comprising at least one cationic biocidal active and a cationic fabric softener.
15. 15 A method of stabilising a cationic liquid dispersion containing at least one cationic surfactant, comprising adding an unsaturated fatty alcohol emulsifier to a liquid dispersion containing the at least one cationic surfactant, preferably wherein the at least one cationic surfactant is a mixture of at least one cationic biocide and at least one cationic fabric softener.-23 -