GB2608507A - Chinese herbal medicine composition for reducing weight and lowering blood sugar and its preparation - Google Patents
Chinese herbal medicine composition for reducing weight and lowering blood sugar and its preparation Download PDFInfo
- Publication number
- GB2608507A GB2608507A GB2208875.1A GB202208875A GB2608507A GB 2608507 A GB2608507 A GB 2608507A GB 202208875 A GB202208875 A GB 202208875A GB 2608507 A GB2608507 A GB 2608507A
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- blood sugar
- weight
- aqueous solution
- lowering blood
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Abstract
A Chinese herbal medicine composition for reducing weight and lowering blood sugar and a preparation thereof are provided, where the composition specifically comprises the following Chinese herbal medicines as raw materials in parts by weight: 25-30 parts of Pericarpium Citri Reticulatae (Chenpi, dried orange peel), 8-12 parts of Cassia obtusifolia (Senna obtusifolia) seed, 10-13 parts of Houttuynia Cordata, 19-24 parts of Exocarpium Benincasae (winter melon peel), 9-12 parts of lotus leaf base, 10-12 parts of Acanthopanax gracilistylus (Eleutherococcus gracilistylus) and 10-15 parts of Vigna umbellata. According to the present application, the effective components in the Chinese medicine ingredients are extracted by using aqueous solutions with different pH values, and the combined extractions are used to prepare the Chinese medicine preparations for reducing weight and lowering blood sugar. The Chinese herbal medicine composition for reducing weight and lowering blood sugar disclosed by the present application not only has the effects of reducing weight and lowering blood sugar, but also can protect blood vessels.
Description
Chinese Herbal Medicine Composition for Reducing Weight and Lowering Blood Sugar and its Preparation
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority to Chinese Patent Application No. 202110695744X, filed on June 23, 2021, the contents of which are hereby incorporated by reference. TECHNICAL FIELD The present application relates to the technical field of traditional Chinese medicine for weight loss, and in particular to a Chinese herbal medicine composition for reducing weight and lowering blood sugar and a preparation thereof
BACKGROUND
Obesity and type 2 diabetes have become a global phenomenon as a result of changes in diet and lifestyle, for instance, high-fat foods such as pizza, burgers, high-fat meat products, chocolate, fried foods, sweets, pastries, milk tea and biscuits are becoming increasingly popular among consumers, while long-term consumption of such food can easily lead to obesity and further induce various chronic diseases; to make it worse, many people have no time to exercise, making drugs becoming more popular in weight control than exercise.
Among various kinds of weight loss drugs available on the market, the only nationally approved drug that is safe for weight loss is orlistat, an inhibitor of gastrointestinal lipase; orlistat binds to gastric lipase and pancreatic lipase in the stomach and small intestine to inactivate enzymes, where inactivated enzymes can no longer decompose the fat in food into absorbable nutrients, and the undigested fat will hence be excreted through the gastrointestinal tract, thus reducing the calorie intake. Orlistat has a relatively good effect on abdominal obesity in men; the drug, however, has some side effects, mainly oily stools, uncontrolled bowel movements, and some patients may also experience gastrointestinal side effects such as nausea.
Chinese herbal medicine is rather safe for weight control and has fewer side effects as comparing to Western medicine; currently, tea-based products for reducing weight are commonly used though the effect of tea-based weight loss products is rather small and less obvious, a Chinese herbal medicine composition with significant weight loss effects is therefore an urgent technical problem in the field to be solved.
SUMMARY
The present application provides a Chinese herbal medicine composition for reducing weight and lowering blood sugar and a preparation thereof; s a herbal medicine composition has the effects of significantly alleviating obesity caused by high-fat diet in addition to lowering blood sugar.
To achieve the above objectives, the present application provides the following technical scheme: one of the technical schemes of the present application provides a Chinese herbal medicine composition for reducing weight and lowering blood sugar, where the composition comprises the following Chinese herbal medicine as raw materials in parts by weight: 25 -30 parts of Per/carpi/1m Citri Retictdatae, 8 -12 parts of Cassia obtusifblia seed, 10 -13 parts of Houttuyniu Cordata, 19 -24 parts of Eicocarpium Benincasae, 9 -12 parts of lotus leaf base, 10 -12 parts of Acanthopanax gracilislylus and 10 -15 parts of fr'igna umbellota.
In an embodiment, the raw materials comprise the following components in parts by weight: 25 parts of Per/carp/urn ('itri Reticulatae, 10 parts of Cassia obtusifblia seed, 10 parts of Houttiollia Cordata, 20 parts of Exoectrpium Benincasae, 10 parts of lotus leaf base, 10 parts of Aectnthopcmax gracilisothrs and 15 parts of Vi pia umbellata. Pericarpium Old Reliculatoe: warm in nature, pungent and bitter in taste; pharmacological effects: regulating Qi and strengthening spleen, regulating middle energizer, eliminating dampness and resolving phlegm; Per/carp/urn Curl Reticularae can be used for abdominal distension, pain and indigestion due to spleen and stomach Qi stagnation, stuffy chest, dullness and loose stools due to dampness and obstruction in the middle energizer, and cough and asthma caused by phlegm and dampness in the lung; the warm nature activate Qi, and the taste of pungent and bitter promotes Qi and water circulation.
Ccrssict obrusiblia seed sweet and bitter in taste, cold in nature, slightly salt; pharmacological effects: clearing liver and improving eyesight, moistening intestines and relaxing bowels; Cassia obrusifolia seed can be used for red eyes, astringent pain, photophobia and tearfulness, headache, dizziness, blurred vision and constipation. Houttuynia Cordata: pungent in taste, slightly cold in nature; pharmacological effects: clearing away heat and toxic materials, eliminating carbuncle, expelling pus, inducing diuresis and treating stranguria; Houttuynia Cordella can be used for pulmonary abscess, pus, phlegm-heat, asthma, cough, dysentery, stranguria, carbuncle, swelling and sore.
Exocarphun Benincasue: sweet in taste and cool in nature; with effects of inducing diuresis and reducing swelling, Exocarpium Benincascre can be used for edema and fullness, unfavourable urination, summer heat and thirst, short and red urination.
Lotus leaf base: bitter and flat in taste and nature; pharmacological effects: clearing summer heat and removing dampness, stopping bleeding and calming the foetus, tonifying the middle energizer and benefiting the Qi.
Accmihopanax gracilislyhis: slightly bitter in taste and pungent in nature; pharmacological effects: invigorating spleen, tonifying kidney and tranquilizing mind. Tigna umbellctta: sweet, sour in taste and flat in nature; pharmacological effects: inducing water and swelling, detoxifying and draining pus; Acanthopanax p.m:ills-Oil:is can be used for edema and swelling, swollen feet, jaundice and urinary redness, rheumatic and febrile paralysis, carbuncles and sores, intestinal canker and abdominal pain.
A further technical scheme of the present application provides a traditional Chinese medicine preparation for reducing weight and lowering blood sugar that taking the extract of the weight-reducing and blood-sugar-lowering Chinese herbal medicine composition as an effective component.
Preferably, the traditional Chinese medicine preparation is prepared into a form of any one of oral decotion, granule, tablet or capsule.
Preferably, the extract is extracted according to following steps: crushing and sieving all raw materials, extracting with an aqueous solution with a pH of 1.8 -2.0, and filtering to obtain filter residue a; continuously extracting filter residue a with an aqueous solution with a pH of 6.0 -6.5, filtering to obtain filter residue b; continuously extracting filter residue b with an aqueous solution with a pH of 8.5-9.0, followed by filtration; combining filtrates obtained after three extractions, removing solvent in the supernatant to obtain an extract.
Preferably, the sieving is sieving through a 20 -40 mesh sieve.
Preferably, the pH values of the aqueous solution with a pH of 1.8 -2.0 and the aqueous solution with a pH of 6.0 -6.5 are adjusted by hydrochloric acid; the pH value of the aqueous solution with the pH of 8.5 -9.0 is adjusted by sodium bicarbonate.
Preferably, when extracted with aqueous solution of pH 1.8 -2.0, an extraction duration is 120 -140 minutes (min), where the aqueous solution is used in an amount of 10 times the dry weight of the Chinese herbal medicine; when extracted with aqueous solution of pH 6.0 -6.5, an extraction duration is 60 -70 min, where the aqueous solution is used in an amount of 8 times the dry weight of the Chinese herbal medicine; when extracted with aqueous solution of pH 8.5 -9.0, an extraction duration is 30 -35 mm, and aqueous solution is used in an amount of 8 times the dry weight of the Chinese herbal medicine.
The present application has the following beneficial technical effects: the Chinese herbal medicine composition for reducing weight and lowering blood sugar can control body weight by inhibiting absorption of fat from food; the Chinese herbal medicine composition for reducing weight and lowering blood sugar also has the functions of reducing blood sugar and protecting blood vessels. BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 shows pictures of three groups of mice after feeding for 12 weeks in vivo experiment, in which a is normal diet group, b is high-fat diet group and c is drug group.
FIG. 2 illustrates the average weight of three groups of mice from 0 to 12 weeks in vivo experiment.
FIG. 3 shows the average food intake of three groups of mice for 1 -12 weeks in vivo experiment.
FIG. 4 shows the food intake and weight gain of three groups of mice in vivo experiment, in which a illustrates the weight gain of three groups of mice, and b illustrates the food efficiency ratio of three groups of mice.
FIG. 5 shows the fat content in the dry feces of mice in high-fat diet group and drug group collected at the 2, 5111 and 101h week in vivo experiment.
FIG. 6 shows the content of total cholesterol in serum of three groups of mice in vivo experiment.
FIG. 7 shows the content of triglyceride in serum of three groups of mice in vivo experiment.
FIG. 8 shows high density lipoprotein (HDL) and low-density lipoprotein (LDL)/yery low-density lipoprotein (VLDL) in serum of three groups of mice in vivo experiment, where a shows HDL in serum of three groups of mice and b shows LDL/VLDL in serum of three groups of mice FIG. 9 shows the weight of liver tissue of three groups of mice in vivo experiment. FIG. 10 shows the weight of white adipose tissue of three groups of mice in viva experiment.
FIG. 11 shows the weight of kidney tissue of three groups of mice in viva experiment. FIG. 12 shows the weight of spleen tissue of three groups of mice in vivo experiment.
FIG. 13 shows liver tissue of a mouse selected from each of three groups of mice in vivo experiment.
FIG. 14 shows slice of liver tissue of a mouse selected from each of three groups of mice in vivo experiment.
FIG. 15 shows a slice of white adipose tissue of one mouse selected from each of the three groups in vivo experiment.
FIG. 16 illustrates a section of kidney tissue of a mouse selected from each of three groups of mice in vivo experiment.
FIG. 17 shows a slice of spleen tissue of one mouse selected from each of the three groups in vivo experiment.
DETAILED DESCRIPTION OF THE EMBODINIENTS
Now, various exemplary embodiments of the present present application will be described in detail. This detailed description should not be taken as a limitation of the present present application, but should be understood as a more detailed description of some aspects, characteristics and embodiments of the present present application. It should be understood that the terms mentioned in the present present application are only used to describe specific embodiments, and are not used to limit the present present application.
In addition, for the numerical range in the present present application, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Every smaller range between any stated value or the intermediate value within the stated range and any other stated value or the intermediate value within the stated range is also included in the present present application. The upper and lower limits of these smaller ranges can be independently included or excluded from the range.
Unless otherwise stated, all technical arid scientific terms used herein have the same meanings commonly understood by those of ordinary skill in the field to which this present application relates. Although the present present application only describes preferred methods and materials, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present present application.
As used in this paper, the terms "comprising", "including", "having" and "containing" are all open terms, meaning including but not limited to.
Embodiment I Drying Per/carp/urn Carl Reticulatae, Cassia abitt.stfolia seed, Houtittynia Gardena, Exocarpium Benincasae, lotus leaf base, Acanthopanax gracilistylus and TIgna umbellata in an oven at 105 degree Celsius (°C) for 4 hours (h), respectively crushing into powders, sieving with a 20-40 mesh sieve; weighing the sieved powders according to a weight ratio of 2.5 kilogams (kg) of Per/carp/urn Carl Renculaiae, kg of Cassia obtusifolia seed, 1 kg of Houttuynict Cordata, 2 kg of Exocarpi urn Benincasae, 1 kg of lotus leaf base, 1 kg of Acatithopanar gracilistylus and 1.5 kg of Vigna umbellata, and adding the powders into a extraction tank; adding an aqueous solution with a pH of 2.0 (adjusted with hydrochloric acid) and amount of 10 times the weight of the medicinal materials, heating and refluxing for 140 minutes (min), and filtering extractive solution; heating residue and refluxing for 70 min with an aqueous solution with pH 6.0 (adjusted by hydrochloric acid) which is 8 times the weight of the medicinal materials, filtering extractive solution; again heating and refluxing residue for 35 min with an aqueous solution of pH 8.5 (adjusted by sodium bicarbonate) which is 8 times the weight of the medicinal materials, filtering extractive solution; combining filtrates obtained from above three extractions, centrifuging at rotation speed of 2,000 revolutions per minute (rpm) for 10 min, then concentrating supernatant under reduced pressure into an extractum, where the extractum is freeze-dried to obtain an extract of 1.48 kg with an extraction rate of 14.8%.
Embodiment 2 Drying Per/carp/urn Curl Retie/Maine, Cassia obtustfidia seed, Houttuynia Cantata, Exocarpium Benincasae, lotus leaf base, Accinthopanax gracilistyhts and Vigna umbellata in an oven at 105°C for 4 h, respectively crushing into powders, and sieving with a 20-40 mesh sieve; weighing the sieved powders according to a weight ratio of 3 kg of Per/carp/um C//r/ Reticutatae, 0.8 kg of Cassia ablustfatia seed, 1.3 kg of HouttuyniaCardata, 1.9 kg of Exocarpium I3enincasae, 1.2 kg of lotus leaf base, 1 kg of Acanthapanax gracilistylus and 1.3 kg of Vigna umbellata, and adding the weighted powders into a extraction tank; adding an aqueous solution with a p1-1 of 1.8 (adjusted by with hydrochloric acid) which is 10 times the weight of the medicinal materials, heating and refluxing for 140 min, and filtering extractive solution; heating residue and refluxing for 60 min with an aqueous solution of pH 6.0 (adjusted by hydrochloric acid) which is 8 times the weight of the medicinal materials, and filtering extractive solution; again heating residue and refluxing for 30 min with an aqueous solution of pH 9.0 (adjusted by sodium bicarbonate) which is 8 times the weight of the medicinal materials, and filtering extractive solution; combining filtrates obtained from above three extractions, centrifuging at rotating speed of 1,800 rpm for 13 min; then concentrating supernatant under reduced pressure into an extractum, where the extractum is freeze-dried to obtain an extract of 1.55 kg with an extraction rate of 14.62%.
Embodiment 3 Drying Pericativium C1117 Reticulatae, Cassia obtusifolia seed, Houttitynict Cordakt, Erocarpium Benincasae, lotus leaf base, Accmthopanar.,7-mcilistylits and Vigna umbellata in an oven at 105°C for 4 h, drying, respectively crushing, and sieving with a 20 -40 mesh sieve; weighing the sieved powder according to the weight ratio of 2.8 kg of Pericarpitatt Citri Reticulcnae, 1.2 kg of Cassia obtustfolia seed, 1.1 kg of Houttuynia (.'orciata, 2.4 kg of Exocarpitim Benincasae, 0.9 kg of lotus leaf base, 1.2 kg of,Alcarnthopanax gracilistylus and 1 kg of Vigna umbellata, and adding the weighted powders into a extraction tank; adding an aqueous solution with a pH of 2.0 (adjusted with hydrochloric acid) which is 10 times the weight of the medicinal materials), heating and refluxing for 120 min, and filtering extractive solution; then heating residue and refluxing for 70 min with an aqueous solution with pH 6.5 (adjusted by hydrochloric acid) which is 8 times the weight of the medicinal materials, filtering extractive solution; again heating residue and refluxing for 35 min with an aqueous solution of pH 8.5 (adjusted by sodium bicarbonate) which is 8 times the weight of the medicinal materials, and filtering extractive solution; combining filtrates obtained from the above three extractions, centrifuging at a rotating speed of 1,500 rpm for 15 min; concentrating supernatant after centrifugation under reduced pressure to obtain an extractum, where the extractum is freeze-dried to obtain an extract of 1.59 kg with an extraction rate of 15.0%.
In vitro experiment preparing solutions of different concentrations using extract obtained in Embodiment 1, and measuring antioxidant activities and anti-lipogenesis activities.
(1) Antioxidant activity: determining DPPH radical scavenging activity (see Table 1 for results), ABTS radical scavenging activity (see Table 2 for results) and half maximal inhibitory concentrations (see Table 3 for results) of DPPH radicals and ABTS radicals in solutions of different concentrations of the extracts obtained in Embodiment 1, respectively; Table 1 Activity of Scavenging DPPH Free Radicals Concentration (gg/mL) DPPH free radical scavenging rate (%) 1,250 98.83 625 90.86 312.5 57.83 156.25 34.17 78.125 18.70 39.0625 10.27 19.5313 5.35 9.7656 3.01 4.8828 1.13 2.4414 -0.74 1.2207 0.20 Table 2 Activity of Scavenging ABTS Free Radicals Concentration (pg/mL) ABTS free radical scavenging rate (%) 98.83 90.86 57.83 34.17 12.5 18.70 6.25 10.27 3.125 5.35 1.5625 3.01 0.7813 1.13 0.3906 -0.74 0.1953 0.20 0.09766 -1.21 0.04882 0.02441 --Table 3 Half Maximal Inhibitory Concentrations of DPPH Free Radical and ABTS Free Radical Sample Half maximal inhibitory Half maximal inhibitory concentration of DPPH (IC50) concentration of ABTS (IC50) (itg/mL) (pg/mL) Embodiment 1 56.86 27.52 it can be seen from Table I -Table 3 that with the increase of the concentration of the extract in Embodiment 1, the scavenging rate of DPPH free radicals and ABTS free radicals is rather high, indicating that the Chinese herbal medicine composition of the present present application has antioxidant activity.
In vivo experiment Dividing sixteen C57BL/6 mice into three groups, namely normal diet group (5 mice), high-fat diet group (6 mice) and drug group (5 mice), where the normal diet group is fed with normal diet formula as shown in Table 4, while the high-fat diet group and drug group are fed with high-fat diet formula as shown in Table 4 (see Table 5 for nutritional ingredients of normal diet formula and high-fat diet formula); mice in each group could are allowed to get food and water at will, the drug group is administered twice daily by gavage, where the drug is the extract obtained in Embodiment 1 at a dose of 100 mg/kg each time, and the three groups of mice are fed for a total of 12 weeks.
Table 4 Composition of Experimental Diet Normal diet formula High-fat diet formula Composition (5L79, PMInutritio, LL, Brentwood, USA) Content Composition (D12492, Content (%) research diet USA) (g/kg) Crude fibre 6.7 Casein 200.0 Amino acid 18.4 L-cystine 3.0 Total saturated fatty acid 1.7 Maltodextrin 10 125.0 Total monosaturated fatty acid 1.4 Sucrose 72.8 Starch 33.9 Lard 245.0 Glucose 0.3 Soybean oil 25.0 Fructose 0.3 Cellulose FCC200 50.0 Sucrose 2.0 Mineral mixture 50.0 S10026B Lactose 0.3 Vitamin (choline tartrate) 2.0 Calcium (Ca) 0.85 Vitamin mixture 1.0 V10001C Phosphorus (P) 0.62 Edible bright blue 0.05 (FD&C blue dye) Cholesterol content 103 ppm Total amount 773.85
Table 5
Nutritional ingredient Normal diet High-fat diet formula formula Protein, % g (w/w) (cl/O kcal) 18.0 (21.03) 26.2 (20.0) Carbohydrate, % g (w/w) (94 kcal) 62.7 (65.30) 26.3 (20.0) Fat, % g (w/w) (% kcal) 5.2 (13.67) 34.9 (60.0) Physiological energy value of food, 3.43 5.21 kcal/g (1) The pictures of three groups of mice after feeding for 12 weeks are shown in FIG. 1, in which a is a normal diet group, b is a high-fat diet group, and b is a drug group (one mouse is excluded from data analysis due to abnormal weight gain). As can be seen from FIG. I, the mice in the high-fat diet group are significantly fatter than those in the normal diet group and the drug group, which indicates that the Chinese herbal medicine composition for reducing weight and lowering blood sugar of the present present application is effective in controlling body weight.
(2) During the experiment, the weight of each group of mice is measured every week, and the results are shown in FIG. 2 (taking the high-fat diet group as the control group, the statistical significance is calculated by one-way analysis of variance (ANOVA) and Dunnett multiple comparison test; * in the figure indicates P < 0.05, ** means P < 0.01, and *** means PC 0.001). As can be seen from FIG. 2, the mice in the high-fat diet group gain weight the fastest, followed by the drug group and the normal diet group the slowest, indicating that the weight-loss and hypoglycemic herbal composition of the present invention is effective in controlling weight.
(3) During the experiment, the average weekly food intake of each of the three groups of mice is recorded, and the results are shown in FIG. 3. As can be seen from FIG. 3, food intakes are significantly lower in the high-fat diet group and the drug group compared to the normal diet group.
(4) Food efficiency ratio of each group of mice is calculated according to the food intake and weight gain of the three groups of mice, the results are shown in FIG. 4, in which a shows the weight gain of the three groups of mice, and b shows the food efficiency ratio of the three groups of mice (taking the high-fat diet group as the control group, using one-way ANOVA and Dunnett multiple comparison test to calculate statistical significance; * in the figure indicates PC 0.05, ** means PC 0.01, and *** means P<0.001). As can be seen from FIG. 4, the mice in the drug group gain weight more slowly and have a relatively lower food efficiency ratio compared to the high-fat diet group, indicating that the herbal compositions of the present invention can act to reduce food intake and lower body weight.
(5) The faeces of mice in the high-fat diet and drug groups are collected at the 2"d, 5th and 10th week of the experiment. 0.5-1 g of dried faeces are taken and moistened with 3m1 of water, the faecal fat is extracted using a mixture of methanol: chloroform (1: 2), the fat content in the dried faeces of mice is calculated and and the results are shown in FIG. 5. It can be seen from FIG. 5 that the fat content in the dry feces of mice in the high-fat diet group and the drug group is similar at the 2" and 511-1 week, and the fat content in the dry feces of mice in the drug group is significantly higher than that in the high-fat diet group at the 10th week, which indicates that the Chinese herbal medicine composition for reducing weight and lowering blood sugar is effective in inhibiting mice from absorbing fat.
(6) The blood glucose levels of the three groups of mice are measured at week 0, 3, 6, 9 and 12 of the experiment, and the results are shown in Table 6. Table 6 Group Blood glucose leve (mg/dL) Week 0 Week 3 Week 6 Week 9 Week 12 Normal 88.80+16.5 92.40+9.1 84.00+7.62* 71.80+.6.26* . 79.40+7.99** diet group 6 5 * High-fat 97.50+20.7 99.67+23. 107.50+13.0 114.00+14.0 138.33+15.90 diet group 6 92 8 9 Drug 100.50+23. 96.00+27. 93.50+16.90 109.00+12.1 116.75+8.85* group 78 68 1 Note: in Table 6, the high-fat diet group is taken as the control group, and the statistical significance is calculated by one-way ANOVA and Dunnett multiple comparison test, where * means P <0.05, and *** means P <0.001, As can be seen from Table 6, the blood glucose level of the mice in the drug group slowly changed from being close to that of the mice in the high-fat diet group at the beginning to being lower than that of the mice in the high-fat diet group as the experiment progressed, indicating that the the Chinese herbal medicine composition for reducing weight and lowering blood sugar is effective in lowering blood glucose of mice.
(7) After the experiment, the serum insulin and homeostatic model assessment (HOMA)-insulin resistance (IR) of three groups of mice are measured with results shown in Table 7.
Table 7
Group Serum insulin Serum insulin Blood glucose HOMA-lift (ng/mL) (gIU/mL) (mmol/L) Normal diet 2.00±0.87* 57.60±25.03* 4.41±0.44*** 1090±3.74** group High-fat diet 4.13+1.04 108.46+37.36 7.69+0.88 40.37+10.86 group Drug group 2.37+1.20* 68.31+34.77* 6.49+0.49* 19.26+8.90** Note: in Table 7, the high-fat diet group is taken as the contro group, and the statistical significance is calculated by one-way ANOVA and Dunnett multiple comparison test, where * means P < 0.05, means P < 0.01, and *** means P < 0.001.
As can be seen from Table 7, the drug group shows a significant reduction in serum insulin levels and fasting blood glucose levels compared to the high-fat diet group, indicating that the herbal combination has significant hypoglycaemic effects.
(8) After the experiment, the contents of total cholesterol and triglyceride in serum of three groups of mice are measured, and the blood lipid spectrum of three groups is analyzed. FIG. 6 shows the content of total cholesterol in serum of three groups of mice; FIG. 7 shows the content of triglyceride in serum of three groups of mice; FIG. 8 shows high density lipoprotein (HDL) and low-density lipoprotein (LDL)/very low-density lipoprotein (VLDL) in senim of three groups of mice, in which a is HDL in serum of three groups of mice, and b is LDL/VLDL in serum of three groups of mice (taking high-fat diet group as the control group, using one-way ANOVA and Dunnett multiple comparison test to calculate statistical significance, where * in the figure indicates P < 0.05, ** means P < 0.01, and *** means P < 0.001). It is clear from FIG. 6 -FIG. 8 that the drug group shows a significant reduction in serum total cholesterol and triglyceride levels, an increase in serum HDL levels and a reduction in LDLNLDL compared to the high-fat diet group, indicating that a lipid-lowering effect is achieved in drug group by promoting lipid metabolism.
(9) After the experiment, the serum total cholesterol and serum high density lipoprotein contents of the three groups of mice are measured, and the atherosclerosis index (AI) and protection rate of the Chinese herbal medicine composition for reducing weight and lowering blood sugar of the present application are calculated with results shown in Table 8.
Table 8
Group Atherosclerosis index (Al) Protection rate (%) Normal diet group 0.92+0.50 -High-fat diet group 1.37+0.21 Drug group 0.84+0.17 38.71 The calculation formula of atherosclerosis index in Table 8 is as follows:
TC -HDL
AI - *
HDL
in the formula, TC is the total cholesterol content in serum, and HDL is the high density lipoprotein content in serum; the protection rate (%) is calculated as follows: High-fat diet group Al -Grug group Al Protection rate (%) = X 100%.
High-fat diet group Al as can be seen from Table 8, Al is significantly reduced in the drug group as comparing to the high-fat diet group with protection rate reaching 38.71%, indicating that the Chinese herbal medicine composition of the present application has the effect of lowering blood fat.
(10) After the experiment, three groups of mice are dissected. The weight of liver tissue, white adipose tissue, kidney tissue and spleen tissue in each group are shown in FIG. 9, FIG. 10, FIG. 11 and FIG. 12, respectively (taking the high-fat diet group as the control group, using one-way ANOVA and Dunnett multiple comparison test to calculate the statistical significance, where * in the figure indicates P < 0.05, ** means p < 0.01, *** means P < 0.001). It can be seen from FIG. 9 -FIG. 12 that the drug group shows significant reduction in the weight of liver tissue, white adipose tissue, kidney tissue and spleen tissue compared to the high-fat diet group, indicating that the herbal composition in the present invention can significantly alleviate the effect of obesity in mice on a high-fat diet.
From each of the three groups, one mouse's dissected tissues and organs are selected for sectioning observation. See FIG. 13 for liver tissue pictures of three mice, FIG. 14 for liver tissue slices, FIG. 15 for white fat slices, FIG. 16 for kidney tissues and FIG. 17 for spleen tissues. It can be seen from FIG. 13 -FIG. 17 that the lipid content in liver, kidney and spleen of mice in the drug group is obviously reduced, and the size of adipocytes is obviously reduced, indicating that the Chinese herbal medicine composition of the present application is effective in promoting lipid metabolism.
The specific effect of the extract obtained in Embodiment I is verified as follows: the extract obtained from the extractions of Embodiment 1 is prepared into an oral decoction, and the effect of the decoction is compared with that of an aqueous decoction obtained by the traditional method of water decoction with the herbal composition configured according to the weight ratio of the herbs in Embodiment 1. 30 obesity patients are selected, 15 of whom take an oral decoction made from the extract obtained in Embodiment 1 at a dose containing 100 mg/kg of extract, and are recorded as the Embodiment 1 group; 15 of whom take a aqueous decoction of the configured herbal composition (corresponding weight of each herb calculated according to the extraction rate of Embodiment 1), and are recorded as the control group. The initial weights of the 30 patients are recorded and after 8 weeks of adherence, the weights of the 30 patients are measured again and the changes in weight are calculated and the results are shown in Table 9.
Table 9 Weight Changes of 30 Obesity Patients Average weight at week Average weight at the 8th week (kg) Weight 0 (kg) change (kg) Embodiment 1 96.2 85.3 -10.9 group Control group 94.5 87.2 -7.3 As can be seen from Table 9, the extract obtained using the extraction method of the present application has a better effect on reducing weight compared to the traditional method of water decoction. The reason for this is that the aqueous solutions of different pH values designed by the present invention are close to the pH values of gastric and intestinal fluids in the human body, and the active ingredients obtained from the extraction are better absorbed by the human body, thus providing better results compared to the traditional method of water decoction.
The above-mentioned embodiments only describe the preferred mode of the present application, but do not limit the scope of the present application. On the premise of not departing from the design spirit of the present application, all kinds of modifications and improvements made by ordinary technicians in the field to the technical scheme of the present application shall fall within the scope of protection determined by the claims of the present application.
Claims (8)
- WHAT IS CLAIMED IS: 1. A traditional Chinese herbal medicine composition for reducing weight and lowering blood sugar comprising Chinese herbal medicine as raw materials in parts by weight of 25 -30 parts of Per/carp/um Citri Renculatae, 8 -12 parts of Cassia obiusifolia seed, 10 -13 parts of Houltuyllia Cordata, 19 -24 parts of hicocarpiwn Benincasae, 9 -12 parts of lotus leaf base, 10 -12 parts of Acanthopcmcvc gracilistylus and 10 -15 parts of Vig7la umbellata.
- 2. The traditional Chinese herbal medicine composition for reducing weight and lowering blood sugar according to claim 1, wherein the raw materials comprise in parts by weight of 25 parts of Per/carp/urn CIO Retictdaiae, 10 parts of CaKsia oblusifolia seed, 10 parts of Min i/40410 Cordala, 20 parts of winter melon peel, 10 parts of lotus leaf base, 10 parts of Acwithopctricix gracilistyhts and 15 parts of Trignct umbellata.
- 3. A traditional Chinese medicine preparation for reducing weight arid lowering blood sugar prepared by using extract of the Chinese herbal medicine composition for reducing weight and blood sugar according to claim 1 or 2 as effective component.
- 4. The traditional Chinese medicine preparation for reducing weight and lowering blood sugar according to claim 3, wherein the Chinese medicine preparation is prepared into a form of any one of oral decoti on, granule, tablet or capsule.
- 5. The traditional Chinese medicine preparation for reducing weight and lowering blood sugar according to claim 3 or 4, wherein the extract is obtained by the following steps: crushing and sieving all raw materials, extracting with an aqueous solution with a pH in a range of 1.8 -2.0, and filtering to obtain filter residue a; further extracting filter residue a with an aqueous solution with a pH ranges between 6.0 and 6.5, filtering to obtain filter residue; further extracting filter residue b with an aqueous solution with a pH ranges from 8.5 to 9.0, filtering; combining filtrates obtained after three extractions, and removing solvent in supernatant to obtain the extract.
- 6. The traditional Chinese medicine preparation for reducing weight and lowering blood sugar according to claim 5, wherein the sieving is sieving through a 20 -40 mesh sieve.
- 7. The traditional Chinese medicine preparation for reducing weight and lowering blood sugar according to claim 5, wherein the pH values of the aqueous solution with a pH in a range of 1.8 -2.0 and the aqueous solution with a pH ranges between 6.0 and 6.5 are adjusted by hydrochloric acid; the pH value of the aqueous solution with the pH ranges from 8.5 to 9.0 is adjusted by sodium bicarbonate.
- 8. The Chinese medicine preparation for reducing weight and lowering blood sugar according to claim 5, wherein when extracted with aqueous solution of pH 1.8 -2.0, an extraction duration is 120 -140 minutes (min), and the aqueous solution is used in an amount of 10 times the dry weight of the Chinese herbal medicine; when extracted with aqueous solution of pH 6.0 -6.5, an extraction duration is 60 -70 min, and the aqueous solution is used in an amount of 8 times the dry weight of the Chinese herbal medicine; when extracted with aqueous solution of pH 8.5 -9.0, an extraction duration is 30 -35 min, and aqueous solution is used in an amount of 8 times the dry weight of the Chinese herbal medicine.
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CN105477614A (en) * | 2015-12-29 | 2016-04-13 | 余勇 | Medicine for treating obesity and preparation method of medicine |
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CN107496786A (en) * | 2017-10-08 | 2017-12-22 | 王风连 | The medicinal liquor of lipid-loweringing detumescence |
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CN107496786A (en) * | 2017-10-08 | 2017-12-22 | 王风连 | The medicinal liquor of lipid-loweringing detumescence |
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