GB2594339B - Enzymes with RUVC domains - Google Patents
Enzymes with RUVC domains Download PDFInfo
- Publication number
- GB2594339B GB2594339B GB2010770.2A GB202010770A GB2594339B GB 2594339 B GB2594339 B GB 2594339B GB 202010770 A GB202010770 A GB 202010770A GB 2594339 B GB2594339 B GB 2594339B
- Authority
- GB
- United Kingdom
- Prior art keywords
- enzymes
- ruvc domains
- ruvc
- domains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2219441.9A GB2617659B (en) | 2020-02-14 | 2020-07-13 | Enzymes with RuvC domains |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US2020/018432 WO2020168291A1 (en) | 2019-02-14 | 2020-02-14 | Enzymes with ruvc domains |
US202063022320P | 2020-05-08 | 2020-05-08 |
Publications (3)
Publication Number | Publication Date |
---|---|
GB202010770D0 GB202010770D0 (en) | 2020-08-26 |
GB2594339A GB2594339A (en) | 2021-10-27 |
GB2594339B true GB2594339B (en) | 2023-02-08 |
Family
ID=72140085
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2010770.2A Active GB2594339B (en) | 2020-02-14 | 2020-07-13 | Enzymes with RUVC domains |
GB2219441.9A Active GB2617659B (en) | 2020-02-14 | 2020-07-13 | Enzymes with RuvC domains |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2219441.9A Active GB2617659B (en) | 2020-02-14 | 2020-07-13 | Enzymes with RuvC domains |
Country Status (1)
Country | Link |
---|---|
GB (2) | GB2594339B (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190249200A1 (en) * | 2018-02-15 | 2019-08-15 | Sigma-Aldrich Co., Llc | Engineered cas9 systems for eukaryotic genome modification |
WO2020168291A1 (en) * | 2019-02-14 | 2020-08-20 | Metagenomi Ip Technologies, Llc | Enzymes with ruvc domains |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9944912B2 (en) * | 2015-03-03 | 2018-04-17 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases with altered PAM specificity |
-
2020
- 2020-07-13 GB GB2010770.2A patent/GB2594339B/en active Active
- 2020-07-13 GB GB2219441.9A patent/GB2617659B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190249200A1 (en) * | 2018-02-15 | 2019-08-15 | Sigma-Aldrich Co., Llc | Engineered cas9 systems for eukaryotic genome modification |
WO2020168291A1 (en) * | 2019-02-14 | 2020-08-20 | Metagenomi Ip Technologies, Llc | Enzymes with ruvc domains |
Non-Patent Citations (4)
Title |
---|
Frontiers in Plant Science, Vol. 7, 2016, Ding et. al., "Recent Advances in Genome Editing Using CRISPR/Cas9" * |
Molecular cell, vol. 60, 2015, Shmakov et. al., Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems, pp. 385-397. * |
Nature reviews microbiology, vol. 18, 2020, Makarova et. al., "Evolutionary classification of CRISPRCas systems: a burst of class 2 and derived variants", pp. 67-83 * |
Science, vol. 337, 2012, Jinek at. al., A Programmable Dual-RNA Guided DNA Endonuclease in Adaptive Bacterial Immunity, pp. 816-821. * |
Also Published As
Publication number | Publication date |
---|---|
GB2594339A (en) | 2021-10-27 |
GB2617659A (en) | 2023-10-18 |
GB202219441D0 (en) | 2023-02-01 |
GB2617659B (en) | 2024-05-01 |
GB202010770D0 (en) | 2020-08-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB202217378D0 (en) | Enzymes with ruvc domains | |
CA3241703A1 (en) | Enzymes with ruvc domains | |
EP3875469A4 (en) | Novel crispr/cas12f enzyme and system | |
EP3882345A4 (en) | Crispr-cas12j enzyme and system | |
EP3870697A4 (en) | Engineered enzymes | |
IL289202A (en) | Uridine diphosphate-dependent glycosyltransferase enzyme | |
EP3924477A4 (en) | Enzymes with ruvc domains | |
IL281171A (en) | Cellulose-synthase-like enzymes and uses thereof | |
EP3846938A4 (en) | Immobilized enzymes and microsomes on magnetic scaffolds | |
EP3821000A4 (en) | Engineered galactose oxidase variant enzymes | |
EP3802827A4 (en) | Modified rna agents with reduced off-target effect | |
EP3843774A4 (en) | Cardiac cell reprogramming with myocardin and ascl1 | |
EP3840582A4 (en) | Modification of protein glycosylation in microorganisms | |
EP3821026A4 (en) | Engineered phosphopentomutase variant enzymes | |
EP3938400A4 (en) | Cd22 antibodies and methods of using the same | |
EP4093863A4 (en) | Crispr-cas enzymes with enhanced on-target activity | |
EP3954774A4 (en) | Rna site-directed editing using artificially constructed rna editing enzymes and related uses | |
EP4074837A4 (en) | Modified daao enzyme and application thereof | |
EP3625335A4 (en) | Immobilized enzyme complexes and related methods | |
GB201917688D0 (en) | SHC enzymes and enzyme variants | |
GB2617659B (en) | Enzymes with RuvC domains | |
EP3775255A4 (en) | Metabolic assay for bacterial growth and gram typing | |
EP3827078A4 (en) | Encapsulated microorganisms and methods of using same | |
EP3917970A4 (en) | Engineered microorganisms and methods of making and using same | |
EP3864149A4 (en) | Protease enzyme variants and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
732E | Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977) |
Free format text: REGISTERED BETWEEN 20220317 AND 20220323 |