GB2594339B - Enzymes with RUVC domains - Google Patents

Enzymes with RUVC domains Download PDF

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Publication number
GB2594339B
GB2594339B GB2010770.2A GB202010770A GB2594339B GB 2594339 B GB2594339 B GB 2594339B GB 202010770 A GB202010770 A GB 202010770A GB 2594339 B GB2594339 B GB 2594339B
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United Kingdom
Prior art keywords
enzymes
ruvc domains
ruvc
domains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB2010770.2A
Other versions
GB2594339A (en
GB202010770D0 (en
Inventor
Thomas Brian
Brown Christopher
Kantor Rose
Devoto Audra
Butterfield Cristina
Alexander Lisa
S A Goltsman Daniela
Liu Jason
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Metagenomi Inc
Original Assignee
Metagenomi Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2020/018432 external-priority patent/WO2020168291A1/en
Application filed by Metagenomi Inc filed Critical Metagenomi Inc
Priority to GB2219441.9A priority Critical patent/GB2617659B/en
Publication of GB202010770D0 publication Critical patent/GB202010770D0/en
Publication of GB2594339A publication Critical patent/GB2594339A/en
Application granted granted Critical
Publication of GB2594339B publication Critical patent/GB2594339B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB2010770.2A 2020-02-14 2020-07-13 Enzymes with RUVC domains Active GB2594339B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2219441.9A GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains
US202063022320P 2020-05-08 2020-05-08

Publications (3)

Publication Number Publication Date
GB202010770D0 GB202010770D0 (en) 2020-08-26
GB2594339A GB2594339A (en) 2021-10-27
GB2594339B true GB2594339B (en) 2023-02-08

Family

ID=72140085

Family Applications (2)

Application Number Title Priority Date Filing Date
GB2010770.2A Active GB2594339B (en) 2020-02-14 2020-07-13 Enzymes with RUVC domains
GB2219441.9A Active GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB2219441.9A Active GB2617659B (en) 2020-02-14 2020-07-13 Enzymes with RuvC domains

Country Status (1)

Country Link
GB (2) GB2594339B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190249200A1 (en) * 2018-02-15 2019-08-15 Sigma-Aldrich Co., Llc Engineered cas9 systems for eukaryotic genome modification
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9944912B2 (en) * 2015-03-03 2018-04-17 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases with altered PAM specificity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190249200A1 (en) * 2018-02-15 2019-08-15 Sigma-Aldrich Co., Llc Engineered cas9 systems for eukaryotic genome modification
WO2020168291A1 (en) * 2019-02-14 2020-08-20 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Frontiers in Plant Science, Vol. 7, 2016, Ding et. al., "Recent Advances in Genome Editing Using CRISPR/Cas9" *
Molecular cell, vol. 60, 2015, Shmakov et. al., Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems, pp. 385-397. *
Nature reviews microbiology, vol. 18, 2020, Makarova et. al., "Evolutionary classification of CRISPRCas systems: a burst of class 2 and derived variants", pp. 67-83 *
Science, vol. 337, 2012, Jinek at. al., A Programmable Dual-RNA Guided DNA Endonuclease in Adaptive Bacterial Immunity, pp. 816-821. *

Also Published As

Publication number Publication date
GB2594339A (en) 2021-10-27
GB2617659A (en) 2023-10-18
GB202219441D0 (en) 2023-02-01
GB2617659B (en) 2024-05-01
GB202010770D0 (en) 2020-08-26

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