GB2549819A - Method and device for detection of whole organism bacteria - Google Patents

Method and device for detection of whole organism bacteria

Info

Publication number
GB2549819A
GB2549819A GB1621723.4A GB201621723A GB2549819A GB 2549819 A GB2549819 A GB 2549819A GB 201621723 A GB201621723 A GB 201621723A GB 2549819 A GB2549819 A GB 2549819A
Authority
GB
United Kingdom
Prior art keywords
detection
whole organism
organism bacteria
bacteria
whole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1621723.4A
Other versions
GB2549819B (en
GB201621723D0 (en
Inventor
Govindasamy Klariska
Potgieter Suretha
Land Kevin
Kumar Shavon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council for Scientific and Industrial Research CSIR
Original Assignee
Council for Scientific and Industrial Research CSIR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council for Scientific and Industrial Research CSIR filed Critical Council for Scientific and Industrial Research CSIR
Publication of GB201621723D0 publication Critical patent/GB201621723D0/en
Publication of GB2549819A publication Critical patent/GB2549819A/en
Application granted granted Critical
Publication of GB2549819B publication Critical patent/GB2549819B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides for lateral flow devices, microfluidic devices and methods for detecting whole-organism bacteria in a water sample. The devices and methods provide for the rapid on-site detection of whole-organism bacteria in a water sample.
GB1621723.4A 2014-05-30 2015-05-29 Method and device for detection of E. coli Expired - Fee Related GB2549819B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA201403971 2014-05-30
PCT/IB2015/054060 WO2015181790A1 (en) 2014-05-30 2015-05-29 Method and device for detection of whole organism bacteria

Publications (3)

Publication Number Publication Date
GB201621723D0 GB201621723D0 (en) 2017-02-01
GB2549819A true GB2549819A (en) 2017-11-01
GB2549819B GB2549819B (en) 2019-04-17

Family

ID=53499046

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1621723.4A Expired - Fee Related GB2549819B (en) 2014-05-30 2015-05-29 Method and device for detection of E. coli

Country Status (4)

Country Link
ES (2) ES2696757R1 (en)
GB (1) GB2549819B (en)
WO (1) WO2015181790A1 (en)
ZA (1) ZA201607845B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106526176A (en) * 2016-12-05 2017-03-22 无锡艾科瑞思产品设计与研究有限公司 Multifunctional fluorescence-immunochromatography rapid quantitative detection strip
CN110806477A (en) * 2018-08-06 2020-02-18 国家纳米科学中心 Pathogenic bacterium detection test strip, sensor and application thereof
CN115232779A (en) * 2022-07-18 2022-10-25 浙江省农业科学院 Method for inducing salmonella enteritidis to enter VBNC state by utilizing sodium hypochlorite

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065010A2 (en) * 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
US20090253119A1 (en) * 2004-07-29 2009-10-08 Siliang Zhou Lateral flow system and assay
US20130130404A1 (en) * 2011-11-21 2013-05-23 Abaxis, Inc Signal amplification in lateral flow and related immunoassays
WO2013105090A1 (en) * 2012-01-11 2013-07-18 Aptateck Bio Ltd. A versatile lateral flow strip device
US20130236899A1 (en) * 2012-03-09 2013-09-12 Invisible Sentinel, Inc. Methods And Compositions For Detecting Multiple Analytes With A Single Signal
WO2014035270A1 (en) * 2012-08-31 2014-03-06 Wrocławskie Centrum Badań Eit + Spółka Z Organiczona A method of identifying gram-negative bacteria using the maldi-tof ms method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065010A2 (en) * 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
US20090253119A1 (en) * 2004-07-29 2009-10-08 Siliang Zhou Lateral flow system and assay
US20130130404A1 (en) * 2011-11-21 2013-05-23 Abaxis, Inc Signal amplification in lateral flow and related immunoassays
WO2013105090A1 (en) * 2012-01-11 2013-07-18 Aptateck Bio Ltd. A versatile lateral flow strip device
US20130236899A1 (en) * 2012-03-09 2013-09-12 Invisible Sentinel, Inc. Methods And Compositions For Detecting Multiple Analytes With A Single Signal
WO2014035270A1 (en) * 2012-08-31 2014-03-06 Wrocławskie Centrum Badań Eit + Spółka Z Organiczona A method of identifying gram-negative bacteria using the maldi-tof ms method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN-ZHONG LI ET AL, "Paper based point-of-care testing disc for multiplex whole cell bacteria analysis", BIOSENSORS AND BIOELECTRONICS, vol. 26, no. 11, (2011), pages 4342 - 4348 *
Hanno Teiwes, "A PAPER-BASED LATERAL FLOW DEVICE FOR THE DETECTION OF I[alpha]IP VIA ELISA", [2014-01-01)], URL: http://digitalcommons.uri.edu/cgi/viewcontent.cgi?article=1364&context=theses, *
Hans Jorbeck ET AL, "Immunochemistry of Salmonella O-Antigens: Specificity of Rabbit Antibodies against the O-Antigen 4 Determinant Elicited by Whole Bacteria and O-Antigen 4 Specific Saccharide-Protein Conjugates", Journal of Immunology, (1979), page 1376 *

Also Published As

Publication number Publication date
ES2696757R1 (en) 2019-01-21
ES2628677R1 (en) 2017-11-29
ES2696757A2 (en) 2019-01-17
GB2549819B (en) 2019-04-17
GB201621723D0 (en) 2017-02-01
ES2628677B2 (en) 2018-08-09
ZA201607845B (en) 2019-07-31
WO2015181790A1 (en) 2015-12-03
ES2628677A2 (en) 2017-08-03

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20200529