GB2525562B - Methods for purification of biological cells - Google Patents

Methods for purification of biological cells

Info

Publication number
GB2525562B
GB2525562B GB1515255.6A GB201515255A GB2525562B GB 2525562 B GB2525562 B GB 2525562B GB 201515255 A GB201515255 A GB 201515255A GB 2525562 B GB2525562 B GB 2525562B
Authority
GB
United Kingdom
Prior art keywords
purification
methods
biological cells
biological
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1515255.6A
Other versions
GB201515255D0 (en
GB2525562A (en
Inventor
T Gjerde Douglas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB201515255D0 publication Critical patent/GB201515255D0/en
Publication of GB2525562A publication Critical patent/GB2525562A/en
Application granted granted Critical
Publication of GB2525562B publication Critical patent/GB2525562B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804Affinity chromatography
    • B01D15/3809Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04Cell isolation or sorting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12Purification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB1515255.6A 2013-02-15 2014-02-15 Methods for purification of biological cells Active GB2525562B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201361765541P 2013-02-15 2013-02-15
US201361832501P 2013-06-07 2013-06-07
US201361858054P 2013-07-24 2013-07-24
US201361873828P 2013-09-04 2013-09-04
US201361913154P 2013-12-06 2013-12-06
PCT/US2014/016637 WO2014127314A1 (en) 2013-02-15 2014-02-15 Devices and methods for purification of biological cells

Publications (3)

Publication Number Publication Date
GB201515255D0 GB201515255D0 (en) 2015-10-14
GB2525562A GB2525562A (en) 2015-10-28
GB2525562B true GB2525562B (en) 2017-10-11

Family

ID=51354602

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1515255.6A Active GB2525562B (en) 2013-02-15 2014-02-15 Methods for purification of biological cells

Country Status (3)

Country Link
EP (1) EP2956541B1 (en)
GB (1) GB2525562B (en)
WO (1) WO2014127314A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2587768B (en) * 2014-04-03 2021-08-18 T Gjerde Douglas Devices and methods for purification, detection and use of biological cells
WO2016031971A1 (en) * 2014-08-29 2016-03-03 日立化成株式会社 Cell trapping method, method for producing specific cell-trapping device, and method for producing specific cell-containing solution
WO2017223313A1 (en) * 2016-06-22 2017-12-28 Salamifar Ehsan Aptamer-based compositions and methods for extraction and detection of microbial contamination
US11162143B2 (en) 2018-10-21 2021-11-02 The University Of Kansas Methods for generating therapeutic delivery platforms
US20200209233A1 (en) * 2018-12-31 2020-07-02 Saint-Gobain Performance Plastics Corporation Containers Containing Degradable Carriers
CN114641580A (en) * 2019-07-23 2022-06-17 斯奈普Dna System and method for detecting and monitoring pathogens
US20210405035A1 (en) * 2020-06-30 2021-12-30 The Secant Group, Llc Multifunctional polymeric microsphere/microparticle cell bioreactor system and sorting process

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016116A1 (en) * 1990-04-23 1991-10-31 Cellpro Incorporated Immunoselection device and method
US5773224A (en) * 1996-02-12 1998-06-30 Grandics; Peter Immunoselection system for cell elution
US20050255604A1 (en) * 2004-05-12 2005-11-17 Gjerde Douglas T Method and device for extracting an analyte

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9114383B2 (en) 2003-07-14 2015-08-25 Phynexus, Inc. Method and device for extracting an analyte
US8727132B2 (en) * 2007-12-07 2014-05-20 Miltenyi Biotec Gmbh Sample processing system and methods
US9140697B2 (en) * 2009-03-18 2015-09-22 The Regents Of The University Of California Device for capturing circulating cells
CA3077531C (en) * 2009-06-16 2022-09-20 Genzyme Corporation Improved methods for purification of recombinant aav vectors
CN108531360A (en) * 2011-05-27 2018-09-14 不列颠哥伦比亚大学 Micro-current controlled cell capture for high throughput analysis and analytical equipment

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016116A1 (en) * 1990-04-23 1991-10-31 Cellpro Incorporated Immunoselection device and method
US5773224A (en) * 1996-02-12 1998-06-30 Grandics; Peter Immunoselection system for cell elution
US20050255604A1 (en) * 2004-05-12 2005-11-17 Gjerde Douglas T Method and device for extracting an analyte

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J Exp Med, vol. 144, 1976, Hellstrom et al, 'Fractionation of human T lymphocytes...' pp. 1381-1385 *

Also Published As

Publication number Publication date
WO2014127314A1 (en) 2014-08-21
EP2956541A1 (en) 2015-12-23
EP2956541B1 (en) 2018-12-26
GB201515255D0 (en) 2015-10-14
GB2525562A (en) 2015-10-28
EP2956541A4 (en) 2016-11-30

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Free format text: REGISTERED BETWEEN 20160310 AND 20160316