GB2521893A - Cell migration assay - Google Patents
Cell migration assay Download PDFInfo
- Publication number
- GB2521893A GB2521893A GB1416623.5A GB201416623A GB2521893A GB 2521893 A GB2521893 A GB 2521893A GB 201416623 A GB201416623 A GB 201416623A GB 2521893 A GB2521893 A GB 2521893A
- Authority
- GB
- United Kingdom
- Prior art keywords
- microchannel
- fluid sample
- cell
- cell migration
- access port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000012292 cell migration Effects 0.000 title abstract 2
- 238000010232 migration assay Methods 0.000 title 1
- 239000012530 fluid Substances 0.000 abstract 6
- 239000000758 substrate Substances 0.000 abstract 3
- 238000005259 measurement Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/025—Displaying results or values with integrated means
- B01L2300/028—Graduation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1027—Determining speed or velocity of a particle
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Urology & Nephrology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Optical Measuring Cells (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A cell migration measurement device comprising a substrate member with: ⢠at least one access port in a surface of the substrate adapted to receive a fluid sample; at least one exit port (5) adapted to output fluid sample from the device; ⢠at least one internal surface of the device defining at least one microchannel in the substrate extending from and connected to the access port and the exit port (5) of the device for flow of the fluid sample through the microchannel from the access port to the exit port (5), a cell receiving portion comprising multiple hopper shaped cell receiving portions (3) provided in fluid connection with the microchannel, wherein each of the cell receiving portions (3) provide cells provided in the fluid sample to a constriction portion (7) which is in fluid connection with a cell measuring microchannel (4).
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB1204848.4A GB201204848D0 (en) | 2012-03-20 | 2012-03-20 | Cell migration assay |
PCT/GB2013/050718 WO2013140164A1 (en) | 2012-03-20 | 2013-03-20 | Cell migration assay |
Publications (2)
Publication Number | Publication Date |
---|---|
GB201416623D0 GB201416623D0 (en) | 2014-11-05 |
GB2521893A true GB2521893A (en) | 2015-07-08 |
Family
ID=46052207
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GBGB1204848.4A Ceased GB201204848D0 (en) | 2012-03-20 | 2012-03-20 | Cell migration assay |
GB1416623.5A Withdrawn GB2521893A (en) | 2012-03-20 | 2013-03-20 | Cell migration assay |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GBGB1204848.4A Ceased GB201204848D0 (en) | 2012-03-20 | 2012-03-20 | Cell migration assay |
Country Status (2)
Country | Link |
---|---|
GB (2) | GB201204848D0 (en) |
WO (1) | WO2013140164A1 (en) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1340810A1 (en) * | 2000-12-07 | 2003-09-03 | Effector Cell Institute | Apparatus for treating sample in microamount |
US20060121600A1 (en) * | 2003-04-09 | 2006-06-08 | Shiro Kanegasaki | Apparatus for detecting cell chemotaxis |
US20090155877A1 (en) * | 2004-07-06 | 2009-06-18 | Agency For Science Technology And Research | Biochip for sorting and lysing biological samples |
US20120064505A1 (en) * | 2010-03-22 | 2012-03-15 | Massachusetts Institute Of Technology | Measurement of material properties and related methods and compositions |
-
2012
- 2012-03-20 GB GBGB1204848.4A patent/GB201204848D0/en not_active Ceased
-
2013
- 2013-03-20 WO PCT/GB2013/050718 patent/WO2013140164A1/en active Application Filing
- 2013-03-20 GB GB1416623.5A patent/GB2521893A/en not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1340810A1 (en) * | 2000-12-07 | 2003-09-03 | Effector Cell Institute | Apparatus for treating sample in microamount |
US20060121600A1 (en) * | 2003-04-09 | 2006-06-08 | Shiro Kanegasaki | Apparatus for detecting cell chemotaxis |
US20090155877A1 (en) * | 2004-07-06 | 2009-06-18 | Agency For Science Technology And Research | Biochip for sorting and lysing biological samples |
US20120064505A1 (en) * | 2010-03-22 | 2012-03-15 | Massachusetts Institute Of Technology | Measurement of material properties and related methods and compositions |
Also Published As
Publication number | Publication date |
---|---|
GB201416623D0 (en) | 2014-11-05 |
GB201204848D0 (en) | 2012-05-02 |
WO2013140164A1 (en) | 2013-09-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |