GB2506425A - Electrophoresis imaging system - Google Patents

Electrophoresis imaging system Download PDF

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Publication number
GB2506425A
GB2506425A GB1217435.5A GB201217435A GB2506425A GB 2506425 A GB2506425 A GB 2506425A GB 201217435 A GB201217435 A GB 201217435A GB 2506425 A GB2506425 A GB 2506425A
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GB
United Kingdom
Prior art keywords
base
disposed
electrophoresis
taking device
enlargements
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1217435.5A
Other versions
GB201217435D0 (en
Inventor
Wen-Liang Chuu
Chian-Fa Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Major Science Co Ltd
Original Assignee
Major Science Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Major Science Co Ltd filed Critical Major Science Co Ltd
Priority to GB1217435.5A priority Critical patent/GB2506425A/en
Publication of GB201217435D0 publication Critical patent/GB201217435D0/en
Publication of GB2506425A publication Critical patent/GB2506425A/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G01N27/44778—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis on a common gel carrier, i.e. 2D gel electrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

An electrophoresis system includes a pedestal D1 including a base, two enlargements at both ends of the base each with a cavity therein, and a transparent plate on top of the base; a photographic filter provided on top of the base; a control panel on one enlargement; an electrophoresis tank 5 provided between the enlargements (Fig 9); a covering member A1 releasably provided on the pedestal for concealing the electrophoresis tank; a gel block 7 in the electrophoresis tank for holding DNA samples in place; LED lamps D331 (Fig 7) in each cavity for emitting blue light toward the DNA samples; a hollow mount on the covering member A1 and including a sliding tray; a photographic filter releasably disposed on the tray; and a picture taking device C11 mounted on the hollow mount. A digital camera, CMOS or CCD image sensor may be used.

Description

ELECTROPHORESIS SYSTEM WITH PICTURE TAKING DEVICE
BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention relates to electrophoresis and more particularly to an S electrophoresis system having a picture taking device.
2. Description of Related Art
Electrophoresis is used for a great number of applications. For example, electrophoresis is used for DNA sequencing to determine the genetic composition of a DNA sample. Many existing electrophoresis applications are undertaken using running tanks wherein a sample-containing gel is disposed in an electrophoresis tank in the running tank. Electric current is then applied to the gel by means of an electrode in the running tank to cause electrophoresis of the sample.
Typically, the electrophoresis tank is covered by a lid when energized. Otherwise, it may be unsafe for person operating the electrophoresis system due to radiation concerns. However, the typical safety arrangement is not reliable. Further, step details of the electrophoresis system in operation are not visually available. This means that a person performing the electrophoresis system is not aware of any errors occurred.
As a result, successful electrophoresis cannot be ensured. Thus, the need for improvement still exists.
SUMMARY OF THE INVENTION
It is therefore one object of the invention to provide an electrophoresis system comprising a pedestal comprising a base, two enlargements at both ends of the base respectively, two cavities provided in the enlargements respectively, and a transparent plate provided on a top of the base; a photographic filter member provided on the top of the base; a control panel provided on one enlargement; an electrophoresis tank provided between the enlargements; a covering member releasably provided on the pedestal for concealing the electrophoresis tank; a gel block provided in the electrophoresis tank for holding a plurality of DNA samples in place; a plurality of LED lamps provided in each of the cavities, the LED lamps being for emitting blue light toward the DNA samples; a hollow mount provided on the covering member and comprising a tray slidably disposed in the hollow mount, and a photographic filter S releasably disposed on the tray; and a picture taking device mounted on the hollow mount.
The above and other objects, features and advantages of the invention will become apparent from the following detailed description taken with the accompanying drawings.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a perspective view of an electrophoresis system with a picture taking device according to a first preferred embodiment of the invention; FIG. 2 is an exploded view of FIG. 1 with a pedestal separated; FIG. 3 is a perspective view of a covering member, a hollow mount and a digital cameraofFlG.2; FIG. 4 is an exploded view of FIG. 3; FIG. 5 is a perspective view of the pedestal; FIG. 6 is another perspective view of the pedestal; FIG. 7 is a fragmentary view of FIG. 5 with a second photographic filter and a transparent plate mounted on the base; FIG. 8 is a perspective view of the picture taking device on a table; FIG. 9 is an exploded view similar to FIG. 2 showing an electrophoresis tank mounted on the base; FIG. 10 is a perspective view of DNA samples on top of a gel block of FIG. 9; FIG. 11 is a perspective view of an electrophoresis system with a picture taking device according to a second preferred embodiment of the invention; FIG. 12 is an exploded view of FIG. 11 with a pedestal separated; FIG. 13 is a perspective view of a covering member, a hollow mount and a camera member of FIG. 12; FIG. 14 isan exploded view of FIG. 13; FIG. 15 is a perspective view of the picture taking device and a laptop computer S connected to the picture taking device on a table; and FIG. 16 is an exploded view similar to FIG. 12 showing an electrophoresis tank mounted on the base.
DETAILED DESCRIPTION OF THE INVENTION
Referring to FIGS. ito 10, a picture taking device 1 for electrophoresis system accordance with a first preferred embodiment of the invention comprises the following components as discussed in detail below.
A pedestal Dl is provided and a covering member Al is releasably provided on the pedestal Dl. A hollow mount Bl is provided on top of the covering member Al and comprises a top opening B2, a sliding tray B3 adapted to insert into the hollow mount Bl, a first photographic filter B31 adapted to place on the tray B3, and a support B4 projecting upward from one side of the hollow mount B1. A digital camera Cli has a lower portion inserted into the hollow mount Bl through the opening B2 and is secured by the support B4.
The pedestal Dl has a longitudinal section of U and comprises a rectangular base D2, two enlargements D3 at both ends of the base D2 respectively. A power supply (not shown) is mounted in the base D2. A cavity D33 is provided on an inner surface of each enlargement D2. A plurality of light-emitting diode (LED) lamps D331 for emitting blue light are provided in the cavity D33. A rectangular transparent plate D332 is provided on top of the base D2 and parallel to the enlargements D2. A rectangular second photographic filter D333 is provided on the top of the base D2 and parallel to the transparent plate D332. A control panel D31 powered by the power supply is provided on an inclined top of one enlargement D3. Two openings D32 are provided on a top of the other enlargement D3.
An electrophoresis tank 5 is provided between the enlargements on the base D2 and has electrophoresis electrodes electrically connect to the pedestal Dl by wiring 6 through the openings D32 (i.e., being powered by the power supply). A gel block 7 is S provided on a rectangular top recess of the electrophoresis tank 5 and has a fluorescent dye adapted to be excited by the blue light emitted by the LED lamps D331. A plurality of Deoxyribonucleic acid (DNA) samples 71 are provided on top of the gel block 7 in rows. State changes of the DNA samples 71 can be visually observed by watching the color change of the fluorescent dye in the electrophoresis system. The second photographic filter D333 can eliminate light outer than the blue light out of the illumination of the LED lamps D331 because only blue light is necessary for the excitation of the fluorescent dye.
The covering member Al is secured onto the electrophoresis tank 5 for covering so that the color change of the fluorescent dye of the gel block 7 can be seen clearly for determining whether there are state changes of the DNA samples 71. The provision of the second photographic filter D333 can further ensure correctness of the determination. Lens of the digital camera Cli is disposed in the hollow mount Bi to face the first photographic filter B31 through the opening B2. In the electrophoresis system, a person may view the DNA samples 71 through the lens of the digital camera Cli and further take a picture of the DNA samples 71 as record. Furthermore, the person may immediately stop the electrophoresis system if there is any error. As a result, a successful electrophoresis system can be ensured.
Referring to FIGS. ii to 16, a picture taking device 1 for electrophoresis system in accordance with a second preferred embodiment of the invention is shown. The characteristics of the second preferred embodiment are substantially the same as that of the first preferred embodiment except the following: The digital camera of the second preferred embodiment is replaced with a camera member C12 implemented as a complementary-metal-oxide-semiconductor (CMOS) image sensor or a charge-coupled device (CCD) image sensor. Further, a Universal Serial Bus (USB) cable C2 is provided to connect the camera member Cl 2 to a laptop computer 9. In short, a person operating the picture taking device 1 can S understand the step details of the electrophoresis system by watching what is shown on the screen ot the laptop computer 9 in a relatively easy manner.
While the invention has been described in terms of preferred embodiments, those skilled in the art will recognize that the invention can be practiced with modifications within the spirit and scope of the appended claims. :5

Claims (4)

  1. WHAT IS CLAIMED IS: 1. An electrophoresis system comprising: a pedestal comprising a base, two enlargements at both ends of the base respectively, two cavities disposed in the enlargements respectively, and a S transparent plate disposed on a top of the base; a photographic filter member disposed on the top of the base; a control panel disposed on one enlargement; an electrophoresis tank disposed on the base between the enlargements; a covering member releasably disposed on the pedestal for concealing the electrophoresis tank; a gel block disposed in the electrophoresis tank for holding a plurality of Deoxyribonucleic acid (DNA) samples in place; a plurality of light-emitting diode (LED) lamps disposed in each of the cavities, the LED lamps being for emitting blue light toward the DNA samples; a hollow mount disposed on the covering member and comprising a sliding tray; a photographic filter releasably disposed on the tray; and a picture taking device mounted on the hollow mount.
  2. 2. The electrophoresis system of claim 1, wherein the picture taking device is a digital camera.
  3. 3. The electrophoresis system of claim 1, wherein the picture taking device is a CMSO image sensor.
  4. 4. The electrophoresis system of claim 1, wherein the picture taking device is a COD mage sensor.
GB1217435.5A 2012-09-28 2012-09-28 Electrophoresis imaging system Withdrawn GB2506425A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1217435.5A GB2506425A (en) 2012-09-28 2012-09-28 Electrophoresis imaging system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1217435.5A GB2506425A (en) 2012-09-28 2012-09-28 Electrophoresis imaging system

Publications (2)

Publication Number Publication Date
GB201217435D0 GB201217435D0 (en) 2012-11-14
GB2506425A true GB2506425A (en) 2014-04-02

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Family Applications (1)

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GB1217435.5A Withdrawn GB2506425A (en) 2012-09-28 2012-09-28 Electrophoresis imaging system

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5147522A (en) * 1987-03-16 1992-09-15 Helena Laboratories Corporation Automatic electrophoresis apparatus and method
WO1998000583A1 (en) * 1996-07-03 1998-01-08 Kenning Gregory G Automated electrophoresis and analysis system
US20050103633A1 (en) * 2003-11-18 2005-05-19 Chih-Yuan Tseng Automatic inspection and imaging electrophoresis device
WO2005086586A2 (en) * 2004-03-15 2005-09-22 D.N.R. - Imaging Systems Ltd. Method and system for monitoring material separation process such as electrophoresis process in a sample
WO2005111587A1 (en) * 2004-05-18 2005-11-24 Kabushiki Kaisya Advance Bio-specimen detection device
WO2006086659A2 (en) * 2005-02-10 2006-08-17 Massachusetts Institute Of Technology Column-end fluorescence detection for capillary array electrophoresis
WO2006109902A1 (en) * 2005-04-15 2006-10-19 Technomart Inc. Built-in convertible filter cabinet in gel image analyzer
WO2011025781A1 (en) * 2009-08-24 2011-03-03 Life Technologies Corporation Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5147522A (en) * 1987-03-16 1992-09-15 Helena Laboratories Corporation Automatic electrophoresis apparatus and method
WO1998000583A1 (en) * 1996-07-03 1998-01-08 Kenning Gregory G Automated electrophoresis and analysis system
US20050103633A1 (en) * 2003-11-18 2005-05-19 Chih-Yuan Tseng Automatic inspection and imaging electrophoresis device
WO2005086586A2 (en) * 2004-03-15 2005-09-22 D.N.R. - Imaging Systems Ltd. Method and system for monitoring material separation process such as electrophoresis process in a sample
WO2005111587A1 (en) * 2004-05-18 2005-11-24 Kabushiki Kaisya Advance Bio-specimen detection device
WO2006086659A2 (en) * 2005-02-10 2006-08-17 Massachusetts Institute Of Technology Column-end fluorescence detection for capillary array electrophoresis
WO2006109902A1 (en) * 2005-04-15 2006-10-19 Technomart Inc. Built-in convertible filter cabinet in gel image analyzer
WO2011025781A1 (en) * 2009-08-24 2011-03-03 Life Technologies Corporation Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials

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Publication number Publication date
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