GB2442271B - An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells - Google Patents
An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cellsInfo
- Publication number
- GB2442271B GB2442271B GB0619005A GB0619005A GB2442271B GB 2442271 B GB2442271 B GB 2442271B GB 0619005 A GB0619005 A GB 0619005A GB 0619005 A GB0619005 A GB 0619005A GB 2442271 B GB2442271 B GB 2442271B
- Authority
- GB
- United Kingdom
- Prior art keywords
- directly uses
- assay protocol
- dermal cells
- cellular interactions
- natural cellular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003556 assay Methods 0.000 title 1
- 230000008614 cellular interaction Effects 0.000 title 1
- 210000001339 epidermal cell Anatomy 0.000 title 1
- 238000000338 in vitro Methods 0.000 title 1
- 210000003491 skin Anatomy 0.000 title 1
- 210000004927 skin cell Anatomy 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Dermatology (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0619005A GB2442271B (en) | 2006-09-27 | 2006-09-27 | An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0619005A GB2442271B (en) | 2006-09-27 | 2006-09-27 | An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells |
Publications (3)
Publication Number | Publication Date |
---|---|
GB0619005D0 GB0619005D0 (en) | 2006-11-08 |
GB2442271A GB2442271A (en) | 2008-04-02 |
GB2442271B true GB2442271B (en) | 2011-06-15 |
Family
ID=37434738
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB0619005A Expired - Fee Related GB2442271B (en) | 2006-09-27 | 2006-09-27 | An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB2442271B (en) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1103601A1 (en) * | 1999-11-26 | 2001-05-30 | Menicon Co., Ltd. | A method for culturing cell and a culture vessel |
US20020028192A1 (en) * | 1998-03-24 | 2002-03-07 | S. Dan Dimitrijevich | Non-contracting tissue equivalent |
WO2002046375A1 (en) * | 2000-12-08 | 2002-06-13 | Xgene Corporation | In vitro synthesis of a layered cell sorted tissue |
EP1312669A1 (en) * | 2000-07-21 | 2003-05-21 | Cellseed Inc. | Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same |
EP1314440A2 (en) * | 2001-11-22 | 2003-05-28 | Nipro Corporation | Cultured skin and method of maufacturing the same |
WO2003051419A1 (en) * | 2001-12-19 | 2003-06-26 | Henkel Kommanditgesellschaft Auf Aktien | Skin/hair equivalent with reconstructed papillae |
-
2006
- 2006-09-27 GB GB0619005A patent/GB2442271B/en not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020028192A1 (en) * | 1998-03-24 | 2002-03-07 | S. Dan Dimitrijevich | Non-contracting tissue equivalent |
EP1103601A1 (en) * | 1999-11-26 | 2001-05-30 | Menicon Co., Ltd. | A method for culturing cell and a culture vessel |
EP1312669A1 (en) * | 2000-07-21 | 2003-05-21 | Cellseed Inc. | Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same |
WO2002046375A1 (en) * | 2000-12-08 | 2002-06-13 | Xgene Corporation | In vitro synthesis of a layered cell sorted tissue |
EP1314440A2 (en) * | 2001-11-22 | 2003-05-28 | Nipro Corporation | Cultured skin and method of maufacturing the same |
WO2003051419A1 (en) * | 2001-12-19 | 2003-06-26 | Henkel Kommanditgesellschaft Auf Aktien | Skin/hair equivalent with reconstructed papillae |
Non-Patent Citations (1)
Title |
---|
Int J Exp Path, Vol 83, 2002, DE Solomon, "An in vitro examination of an extracellular matrix scaffold for use in wound healing", 209-216 * |
Also Published As
Publication number | Publication date |
---|---|
GB2442271A (en) | 2008-04-02 |
GB0619005D0 (en) | 2006-11-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 20150927 |