GB2442271B - An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells - Google Patents

An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells

Info

Publication number
GB2442271B
GB2442271B GB0619005A GB0619005A GB2442271B GB 2442271 B GB2442271 B GB 2442271B GB 0619005 A GB0619005 A GB 0619005A GB 0619005 A GB0619005 A GB 0619005A GB 2442271 B GB2442271 B GB 2442271B
Authority
GB
United Kingdom
Prior art keywords
directly uses
assay protocol
dermal cells
cellular interactions
natural cellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0619005A
Other versions
GB2442271A (en
GB0619005D0 (en
Inventor
Denis Eon Solomon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to GB0619005A priority Critical patent/GB2442271B/en
Publication of GB0619005D0 publication Critical patent/GB0619005D0/en
Publication of GB2442271A publication Critical patent/GB2442271A/en
Application granted granted Critical
Publication of GB2442271B publication Critical patent/GB2442271B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Dermatology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB0619005A 2006-09-27 2006-09-27 An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells Expired - Fee Related GB2442271B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB0619005A GB2442271B (en) 2006-09-27 2006-09-27 An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0619005A GB2442271B (en) 2006-09-27 2006-09-27 An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells

Publications (3)

Publication Number Publication Date
GB0619005D0 GB0619005D0 (en) 2006-11-08
GB2442271A GB2442271A (en) 2008-04-02
GB2442271B true GB2442271B (en) 2011-06-15

Family

ID=37434738

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0619005A Expired - Fee Related GB2442271B (en) 2006-09-27 2006-09-27 An in vitro human skin assay protocol which directly uses the natural cellular interactions between un-manipulated autologous human epidermal and dermal cells

Country Status (1)

Country Link
GB (1) GB2442271B (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1103601A1 (en) * 1999-11-26 2001-05-30 Menicon Co., Ltd. A method for culturing cell and a culture vessel
US20020028192A1 (en) * 1998-03-24 2002-03-07 S. Dan Dimitrijevich Non-contracting tissue equivalent
WO2002046375A1 (en) * 2000-12-08 2002-06-13 Xgene Corporation In vitro synthesis of a layered cell sorted tissue
EP1312669A1 (en) * 2000-07-21 2003-05-21 Cellseed Inc. Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same
EP1314440A2 (en) * 2001-11-22 2003-05-28 Nipro Corporation Cultured skin and method of maufacturing the same
WO2003051419A1 (en) * 2001-12-19 2003-06-26 Henkel Kommanditgesellschaft Auf Aktien Skin/hair equivalent with reconstructed papillae

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020028192A1 (en) * 1998-03-24 2002-03-07 S. Dan Dimitrijevich Non-contracting tissue equivalent
EP1103601A1 (en) * 1999-11-26 2001-05-30 Menicon Co., Ltd. A method for culturing cell and a culture vessel
EP1312669A1 (en) * 2000-07-21 2003-05-21 Cellseed Inc. Cultured epidermal cell sheet, laminated cultured skin sheet and process for producing the same
WO2002046375A1 (en) * 2000-12-08 2002-06-13 Xgene Corporation In vitro synthesis of a layered cell sorted tissue
EP1314440A2 (en) * 2001-11-22 2003-05-28 Nipro Corporation Cultured skin and method of maufacturing the same
WO2003051419A1 (en) * 2001-12-19 2003-06-26 Henkel Kommanditgesellschaft Auf Aktien Skin/hair equivalent with reconstructed papillae

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Int J Exp Path, Vol 83, 2002, DE Solomon, "An in vitro examination of an extracellular matrix scaffold for use in wound healing", 209-216 *

Also Published As

Publication number Publication date
GB2442271A (en) 2008-04-02
GB0619005D0 (en) 2006-11-08

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20150927