GB2440554A - Prediction of the onset of type 1 diabetes. - Google Patents
Prediction of the onset of type 1 diabetes. Download PDFInfo
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- GB2440554A GB2440554A GB0615063A GB0615063A GB2440554A GB 2440554 A GB2440554 A GB 2440554A GB 0615063 A GB0615063 A GB 0615063A GB 0615063 A GB0615063 A GB 0615063A GB 2440554 A GB2440554 A GB 2440554A
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Abstract
An in vitro method for predicting the onset of type 1 diabetes (T1D) in a subject, comprises the steps of: (a) measuring the concentration of at least one amino acid, amino acid derivative or amino acid metabolite in a biological sample taken from the subject; (b) determining the subject's HLA genotype; (c) assigning the subject's genetic risk of developing T1D on the basis of the subject's HLA genotype; (d) combining the information obtained in step (a) with the information in step (c); and (e) predicting the likelihood of onset of T1D based upon the combination of step (d). The diagnostic method can be used to select target subjects for T1D prophylactic treatment, and as part of a T1D preventative treatment regime for neonates having a likelihood of developing childhood T1D.
Description
<p>Prediction and Prophylactic Treatment of Type 1 Diabetes This invention
relates to methods for the prediction of type 1 diabetes, and in particular, to a method for predicting the probable onset of type I diabetes in children by measuring circulating levels of metabolites in blood, and also to methods for the prophylactic treatment of type 1 diabetes.</p>
<p>Diabetes mellitus ("diabetes") is a medical disorder characterised by persistent variable hyperglycemia (i.e. high blood sugar levels). It can result from either inadequate secretion of the hormone insulin, an inadequate response by the body to insulin, or a combination of these factors. The most common forms of diabetes are type 1, type 2 and gestational diabetes.</p>
<p>Type 1 diabetes (T1D, also known as "childhood", "juvenile" or "insulin-dependent" diabetes) is most commonly diagnosed in children and adolescents. It is an autoimmune disorder, in which the patient's own immune system attacks the beta cells in the Islets of Langerhans of the pancreas -where insulin is produced, and/or the insulin molecule itself. Consequently, patients with clinical Ti 0 require regular insulin replacement therapy, immunosuppression treatment or even more experimental therapies such as islet transplantation and stem cell therapy. However, none of these treatments are ideal. While insulin replacement therapy and immunosuppression require longterm treatment and cannot irradicate all diabetes induced complications, islet transplantation and stem cell therapy are not widely available and are very expensive.</p>
<p>Throughout the world the percentage incidence of Ti D is increasing, and this is especially true of the early onset form of Ti D, which affects children under the age of 4 yrs. Currently in North America Ti D affects approximately i in 300 people and accounts for approximately 10% of all diabetic cases.</p>
<p>Ti D is a multigenetic disorder, with up to 20 genes known to contribute, Of these, the major genetic susceptibility determinants are the human leukocyte antigen (HLA) class II alleles, HLA-DR and HLA-DQ, which are associated with up to 50% of all Ti D cases. However, genetic factors alone cannot account for a person's susceptability to the disease. For instance, less than 10% of new T1D patients have an affected family member, while the concordance between genetically identical twins is only approximately 50%.</p>
<p>In addition, the incidence of Ti D is rising far too rapidly to be attributed merely to the inheritance of diabetogenic genes. Thus, it is widely acknowledged that non-genetic, environmental factors (e.g. nutrition, infectious agents) must also play an important role in either the triggering or progression of the disease process, or both.</p>
<p>Indeed, several prospective studies have suggested that factors operating early in life may play an important role in the etiopathogenesis of liD (Akerblom H. K. et a!., 2002, Am. J. Med. Genet. May, 115(1), 18-29).</p>
<p>Amongst the environmental factors that may be involved, dietary factors operating early in life, i.e. short breast feeding, early exposure to cow's milk, early introduction of gluten, have been suggested to increase the risk of developing the disease (Virtanen S. M. & Knip M. 2003, Am. J. Clin. Nutr., Dec., 78(6), 1053-1067; and Ziegler A.G. et al., 2003, J. Am. Med. Assoc., Oct, 290(13), 1721-1728).</p>
<p>The non-genetic factors that contribute to Ti D susceptibility are even less well defined than the genetic factors. In particular, there is little verifiable evidence of the specific contribution of non-genetic factors to Ti D or how such factors interact with and influence the known genetic predeterminants.</p>
<p>The clinical onset (i.e. the insulin-dependent stage) of Ti D is preceded by a sub-clinical phase, that can last up to several years, during which the insulin-producing islet cells and/or the patient's insulin are progressively destroyed.</p>
<p>The sub-clinical phase is typically characterised by the presence of autoantibodies, which target one or more of the subject's islet cells (islet cell antibodies, ICAs), insulin (insulin autoantibodies, IAAs), glutamic acid decarboxylase (glutamic acid decarboxylase autoantibodies, GADAs) and tyrosine phosphatase (tyrosine phosphatase autoantibodjes, IA-2As).</p>
<p>Accordingly, there is potentially a window of opportunity during which subjects at risk of Ti D may be identified for preventative therapy.</p>
<p>Increasingly, combinations of markers are being used to better define the risk of diabetes. However, while the detection of two or more types of autoantibody (ICA, IAA, GADA and lA-2A) has been linked to the future onset of diabetes, significant problems remain in the selection and identification of subjects having a high probability of developing T1D, and especially of children vulnerable to the early-onset form.</p>
<p>In this regard, until now, the selection of candidates for Ti D predictive testing has largely focussed on those subjects who have a family member already with the disease. However, with a less than 10% familial correlation, this approach is entirely inadequate to provide an effective means of identifying 90% of eventual Ti D patients for intervention therapy. Hence, there is a need for a reliable, effective and simple method for predicting susceptibility or predisposition to Ti 0, that can be readily used for large-scale screening of the general population, rather than of specific genetic sub-groups.</p>
<p>Although children who develop early-onset Ti D (i.e. in the period 0-4 yrs) may show signs of IAAs even from birth, it is not possible to distinguish neonatal autoantibodies from transplacental maternal IgGs in the first year of life.</p>
<p>Accordingly, of those neonatal children tested and found positive for particular autoantigens, the vast majority may not ultimately develop Ti 0.</p>
<p>In view of the rising incidence of Ti D in children, the lack of a suitable method for predicting the likely onset of Ti D in children, and in view of the potential for an early intervention therapy if it is predicted sufficiently early in children, it would be particularly desirable to have an effective method of predicting Ti D in children, especially in the first few days of life.</p>
<p>Moreover, given the current lack of a definitive cure for T1D, the need for frequent, tightly controlled medication, and the potentially fatal consequences of a lapse in the treatment regime, the most important driver for detecting individuals who are at risk of developing Ti D is the potential for a preventative therapy. Thus, it would be desirable to provide a prophylatic treatment regime for preventing or at least delaying the onset of Ti D. This invention aims to overcome or alleviate the problems associated with the</p>
<p>prior art.</p>
<p>In light of the above, in prospective studies into the development of early-onset Ti D, the inventors have surprisingly found that neonatal children who go on to develop Ti D before the age of 4 yrs, display reduced levels of circulating amino acids in the first few days of life (e.g. 0-3 days), compared with children who do not develop Ti D. Significantly, this trend is apparent irrespective of whether the subject has a low, moderate or high genetic predisposition to Ti D based on his or her HLA genotype.</p>
<p>Thus, in accordance with a first aspect of the invention, there is provided an in vitro method for predicting the onset of type 1 diabetes (Ti D) in a subject, comprising the steps of: (a) measuring the concentration of at least one amino acid, amino acid derivative or amino acid metabolite in a biological sample taken from the subject; (b) determining the subject's HLA genotype; (c) assigning the subject's genetic risk of developing Ti D on the basis of the subject's HLA genotype; (d) combining the information obtained in step (a) with the information in step (C); and (e) predicting the likelihood of onset of Ti D based upon the combination of step (d).</p>
<p>The amount of one or more amino acid can be measured in a number of relatively simple, quick and cost-effective ways, making the method of the invention a simple, reliable, quick and economical way of predicting the likelihood of future onset of Ti D in a subject.</p>
<p>Thus, compared with prior art processes for predicting the development of Ti D, the method of the invention has the advantage that it can be performed universally (i.e. on any subject, not just those whom have already been identified as being within a genetically high-risk group).</p>
<p>Indeed, the inventors have surprisingly found that the method of the invention can be used to predict the likelihood of onset of T1D in any member of the population, for example, subjects whom, according to their genetic markers, may be classified as "high", "moderate" / "medium" or "low" risk. Preferably, the genetic risk is classified according to the subject's HLA genotype.</p>
<p>The risk of developing Ti D associated with particular HLA genotypes has been reported by a number of research groups (Redondo M. J. et a!., 2001, Recent Prog. Horm. Res. 56: 69-89; Ilonen J. et a!., 2002, Am. J. Med. Genet.</p>
<p>115(1): 30-6; Mimbacas, A. et a!., 2003, Gen. Mole. Res., 2(1), 29-35; Lambert, A. P. et al., 2004, J. C/in. Endocrinol. Metab., 89(8), 4037-4043; and Buzzetti R. et a!., 2004, Diabetes Metab. Res. Rev., 20(2), 137-143). The skilled person in the art will appreciate that since the method of the invention is applicable to subjects having high, medium or low risk HLA genotypes, the method of assigning such high, medium or low genetic risk categories is not essential to the performance of the invention. Preferably, however, the subject is assigned a genetic risk according to categorisation reported in Buzzettj R. eta!. (2004, Diabetes Metab. Res. Rev., Mar-Apr, 20(2), 137-143), the teaching of which is incorporated herein in its entirety.</p>
<p>Hence, a high risk HLA genotype typically comprises the genetic markers: DRB1 *03 / *Q4 (not 0403), DQB1 0302.</p>
<p>A moderate or medium risk HLA genotype typically comprises the genetic markers: DRB1 *04 (not 0403) / *04 (not 0403), DQB1 0302; DRB1 *04 (not 0403) / X, DQB1 0302 / DQB1 not 0602-3) DRB1 *03 / *03; and DRB1 *03/ X, DQB1 not 0602-3, not 0301, not 0503; wherein X is not DRB1 *03, *04, or 0403.</p>
<p>A low risk HLA genotype comprises any combination of genetic markers not specified above.</p>
<p>In step (a) of the first aspect of the invention, the concentration of one or more amino acid, and/or amino acid derivative and/or amino acid metabolite is measured in a sample taken from a subject.</p>
<p>The term "amino acid" within the scope of the present invention is used in its broadest sense and is meant to include naturally occurring L a-amino acids or residues. The commonly used one and three letter abbreviations for naturally occurring amino acids are used herein (Lehninger, A. L., 1975, Biochemistry, 2d ed., pp. 71-92, Worth Publishers, New York). The general term "amino acid" further includes D-amino acids, where such occur naturally in the human or animal body. The term amino acid also encompasses naturally occurring amino acids that are not usually incorporated into proteins such as norleucine.</p>
<p>There are 20 common, naturally occuring amino acids that form the building blocks of human proteins, namely: alanine (Ala, A), arginine (Arg, R), asparagines (Asn, N), aspartic acid (aspartate, Asp, D), cysteine (Cys, C), glutamic acid (glutamate, Glu, E), glutamine (Gin, Q), glycine (Gly, G), histidine (His, H), isoleucine (lie, I), leucine (Leu, L), lysine (Lys, K), methionjne (Met, M), phenylalanine (Phe, F), proline (Pro, P), serine (Ser, S), threonine (Thr, 1), tryptophan (Trp, W), tyrosine (Tyr, Y), and valine (Val, V).</p>
<p>Eight amino acids are generally regarded as essential for humans: tryptophan, lysine, methjonjne, phenytalanine, threonine, valine, leucine, and isoleucine.</p>
<p>This means that they cannot be synthesised de novo within the human body.</p>
<p>Two others, histidine and arginine are often considered to be essential in children and possibly also in the elderly, because they cannot be synthesised at a sufficient rate to meet the body's needs.</p>
<p>Furthermore, in some case, the distinction between essential and non-essential amino acids is a little blurred, because some amino acids can in fact be produced from others. For example, the sulfur-containing amino acids, methionine and homocysteine, can be converted into each other but neither can be synthesised de novo in humans. Likewise, cysteine can be made from homocysteine, but cannot be synthesised on its own. Hence, for convenience, sulfur-containing amino acids may sometimes be considered as a single pool of nutritionally-equivalent amino acids. Similarly, arginine, ornithine, and citrulline, which are interconvertjble via the urea cycle, are sometimes considered to be a single group of amino acids.</p>
<p>As used herein, the "essential" amino acids are the 8 first listed above, and in the case of a neonate, the essential amino acids also include histidine and argin me.</p>
<p>The remaining 10 or 12 of the above-listed 20 amino acids are considered herein as "non-essential" amino acids in humans, which means that they can be synthesised de novo in the human body.</p>
<p>I</p>
<p>Other examples of amino acids are listed by Roberts and Vellaccio, The Peptides: Analysis, Synthesis, Biology, Gross and Meiehofer, eds., Vol. 5 p. 341, Academic Press, Inc., N.Y. 1983, which is incorporated herein by reference.</p>
<p>As used herein, the terms amino acid "derivative" or "metabolite" refer to naturally occurring, amino acids or small peptides, which do not fall within the amino acids (above) that occur commonly in natural proteins and peptides.</p>
<p>Such derivatives include, for example, chemically modified amino acids, dipeptides (molecules that are based on two covalently bonded amino acids) and tripeptides (molecules that are based on three covalently bonded amino acids), which are typically synthesised by the breakdown of proteins or the assembly of free amino acids in the human body. Examples of amino acid derivatives include camitine, acylcarnitine, glutathione, ornithine, taurine, citrulline, hydroxyproline, gamma-amino-butyric acid, cystine and aminoacetic acid. Amino acids metabolites are naturally occurring compounds that are produced as intermediates or as by-products in the biosynthesis of amino acids or of amino acid derivatives, and include, for example oxoglutarate and creatine. For the purposes of this invention, the terms "derivative" and "metabolite" are not exclusive, in the sense that some amino acid derivatives may also be considered to be amino acid metabolites, e.g. taunne.</p>
<p>Preferably in the methods of the invention, the one or more amino acid, amino acid derivative or amino acid metabolite whose concentration is measured, is selected from the group consisting of: alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalanine, proline, tyrosine and valine.</p>
<p>More preferably, in step (a) of the methods of the invention, the concentration of two or more amino acids, amino acid derivatives or amino acid metabolites are measured. Still more preferably, in step (a) the concentration of essential amino acids or of non-essential amino acids is measured.</p>
<p>Most preferably, the total concentration of all 15 of the amino acids, amino acid derivatives and amino acid metabolites of the above preferable embodiment of the invention is measured.</p>
<p>The concentration of amino acids, amino acid derivatives and amino acid metabolites in a sample taken from a subject can be measured using any suitable means / equipment known to the person of skill in the art.</p>
<p>Conveniently the means will be capable of measuring more than one such amino acids, amino acid derivatives or amino acid metabolites in a single sample at the same time. Advantageously, the technique used will be relatively rapid, for example, capable of generating results (or at least allowing the interpretation of the results) within 24 hours of the start of the procedure.</p>
<p>Preferably, the technique will be capable of analysing samples in a high-throughput manner, to allow the processing of a plurality of samples in a day.</p>
<p>Furthermore, it is beneficial for the equipment used to be widely available so that it is possible to readily analyse samples in appropriate regions of the world. For example, suitable means for measuring the concentrations of amino acids, amino acid derivatives and amino acid metabolites include mass spectrophotometry (e.g. tandem mass spectrometry) and HPLC. Preferably, tandem mass spectrometry is used, for the reasons given above.</p>
<p>In accordance with the invention, a likelihood of onset of Ti D in a subject is predicted on the basis of the measured concentration of one or more amino acid, amino acid derivative or amino acid metabolite in a sample taken from the subject in view of the subject's HLA genotype risk category.</p>
<p>Advantageously, the methods of the invention can be used to predict the onset of Ti D whatever the HLA genotype risk category of the subject, and it is therefore suitable for entire population screening.</p>
<p>The likelihood of onset of Ti D is thus a function of the subject's HLA genotype and the relative concentration of the amino acids, amino acid derivatives or amino acid metabolites. As such, the method provides a distinct advantage over predictions based on a subject's genotype only, which as suggested above can largely ignore non-genetic (e.g. environmental) factors that influence a person's probability of developing Ti D. Thus, where the subject has the high risk HLA genotype (as defined above), a likelihood of onset of Ti D can be predicted when the subject's total concentration of alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, omithine, phenylalanine, proline, tyrosine and valine is less than approximately 1200 j.tmol/L, less than 1100 I.tmol/L, less than 1000 j.imol/L, or less than 900 imol/L.</p>
<p>Preferably, the amino acid concentration in a subject having a high risk HLA genotype is indicative of a likelihood of onset of liD, where the above total amino acid concentration is in the range of 500- 1200 pmol/L, 600-1100 .imol/L, 700-1000 Mmol/L, or 800-900 tmol/L.</p>
<p>Most preferably, the total amino acid concentration for a subject with the high risk HLA genotype is approximately 850.tmoVL.</p>
<p>Where the subject has a medium (moderate) or low risk HLA genotype (as defined above), there is a likelihood of onset of T1D, where the total concentration of the amino acids, amino acid derivatives or amino acid metabolites; alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalan i, proline, tyrosine and valine is less than 1000 i.tmol/L, less than 900 limoVL, less than 800 pmol/L or less than 700 iimol/L.</p>
<p>Preferably, the amino acid concentration in a subject having a medium or low risk HLA genotype is indicative of the likelihood of onset of Ti D, where the above total amino acid concentration is approximately 300-1000 Ilmol/L, 400- 900 limoVL, 500-800 i.tmol/L or 600-700 mol/L.</p>
<p>I Most preferably, the total amino acid concentration for a subject with the medium or low risk HLA genotype is approximately 650 Mmol/L.</p>
<p>The subject's HLA genotype and thus his/her genetic risk of developing Ti D can be determined using any appropriate and available means. For instance, the subject's HLA genotype may already be known, e.g. through familial screening.</p>
<p>Where the HLA genotype must be determined de novo, any suitable means of genetic screening can be used. For example, a sample taken from the subject may be analysed e.g. by PCR, serologic techniques or by DNA microarray technology. Advantageously, the technique used is PCR, for example, as described in Galgani A. et a!. (2004), Human Immunology 65(4) : 366-372. A sample of the subject's DNA may be obtained from the blood and any suitable body tissue or fluid, although preferably the same sample is also used for the measurement of amino acid concentrations.</p>
<p>The inventors have further found that the concentration of camitine (i.e. free carnitine) and/or acylcarnitine can similarly be used in combination with a subjects HLA genotype risk category to predict the likelihood of onset of Ti D. Carnitine (L-carnitine) is a pseudo-amino acid (i.e. a dipeptide) that can be synthesised from protein-bound lysine and methionine residues. However, a human's daily requirement is generally met by food intake. Camitine is known to help regulate fat metabolism and lower cholesterol and triglyceride levels.</p>
<p>It is important in preventing fat build-up in the heart, and it helps to prevent the build-up of ketones (fat waste-products) in the blood. Carnitine is typically interconverted with acylcarnitine (mainly acetyl-L-carnitine), which is involved in the transportation of fatty acids across mitochondrial membranes and is thought to improve cerebral blood flow. Both molecules can be detected in e.g. a human blood sample.</p>
<p>Thus, in a fuher embodiment of the invention, the methods preferably fuher comprise the steps of: (a') measuring the concentration of at least one of, free carnitine, acylcarnitine and total carnitine in a sample taken from said subject; and (d') combining the information obtained in step (a') with the information in step (c); and (e') predicting the likelihood of onset of Ti D based upon the combination of step (d').</p>
<p>The concentration of at least one of, free carnitine, acylcarnitine and total carnitine can be used in the absence of information on the concentration of one or more amino acids to predict the likelihood of onset of Ti D in a subject based on his/her HLA genotype. However, preferably, the method involves the measurement of amino acids (derivatives and metabolites) and of (total) carnitine, such that the prediction of the likelihood of onset of Ti D [step (e')J is based upon the combination of step (d) and the combination of step (d').</p>
<p>More preferably, in step (a') the concentrations of free camitine, acylcamitine and total carnitine are measured.</p>
<p>As in the case of amino acid (derivative and metabolite) concentrations the concentrations of carnitine (free carnitine) and/or acylcarnjtjne can be measured using any appropriate means. Such means are known to the person of skill in the art, and may be selected according to the above-discussed desirable features. Thus, preferably the concentration of carnitine is measured using mass spectrophotomet,.y and more preferably using tandem mass spectrophotometry As in the case of amino acid concentrations, the likelihood of onset of Ti D is a function of the subject's HLA genotype and the relative concentration of his/her carnitine, acylcarnitine and total camitine. Thus, the method provides a further advantage over T1D predictions based solely on a subject's genotype.</p>
<p>Thus, where the subject has the high risk HLA genotype (as defined above), a likelihood of onset of Ti D can be predicted when the subject's total carnitine concentration (which includes the sum of free carnitine and acylcarnitine concentrations) is less than approximately 50.tmol/L, 45 j.imol/L, 42 I.tmol/L, Ilmol/1. or 38 jmol/L.</p>
<p>Preferably, the total carnitine concentration in a subject having a high risk HLA genotype is indicative of a likelihood of onset of Ti D, where the above total amino acid concentration is in the range 20tmol/L, 25-45 tmol/L, 28-42 tmol/L, 30-40 tmol/L or 32-38 mol/L.</p>
<p>Most preferably, the total carnitine concentration for a subject with the high risk HLA genotype is approximately 35 Mmol/L.</p>
<p>Where the subject has the medium or low risk HLA genotype (as defined above), a likelihood of onset of T1D can be predicted when the subject's total carnitine concentration is less than approximately 40 I.lmol/L, 35 pmol/L, 32 imol/L, 30.tmoVL or 28 llmo(/L.</p>
<p>Preferably, where the subject has the medium or low risk HLA genotype, the carnitine concentration is indicative of the likely future onset of Ti D where the total carnitine concentration is in the range 10-40 zmol/L, 15-35 tmol/L, 18-32 .imol/L, 20-30 jmol/L or 22-28 tmol/L.</p>
<p>Most preferably, the total carnitine concentration for a subject with one of the medium or low risk HLA genotypes is approximately 26 p.tmol/L.</p>
<p>Advantageously, a portion or aliquot of the same sample that is used for the measurement of amino acid (derivative and metabolite) concentrations is used</p>
<p>S</p>
<p>for the measurement of free carnitine, acylcarnitine and total carnitine. In this way, multiple samples do not necessarily have to be taken from the same subject. This is particularly beneficial where the subject is a neonate, for whom the taking of multiple samples may be a traumatic experience.</p>
<p>Furthermore, the sample used for measuring amino acid concentrations in step (a), or of carnitine concentrations in step (a') is the same sample that is used for determining the subject's HLA genotype. Thus, preferably a portion or aliquot of the sample used in step (a) is used for genetic screening in step (b).</p>
<p>Preferably, the sample taken from the subject is a body fluid sample, and more preferably the sample is a blood sample. In this regard, the concentrations of amino acids, carnitine and acylcarnitine described herein are reported as tmol per litre of blood. For other body fluids (e.g. urine and saliva) the normal and reduced concentrations of amino acids, camitine and acylcarnitine may have to be adjusted.</p>
<p>Preferably the sample (e.g. blood) has been taken from the subject within 3 days (i.e. 72 hours) of the subject's birth. Thus, the subject is a neonate. As used herein, the term "neonate" refers to a person whom is less than 72 hours old.</p>
<p>A further advantage of the invention is that the measurements of amino acids (derivatives and metabolites) and (total) carnitine concentration can be performed reliably and accurately on a neonate, without the potential inaccuracies / misleading results that can be associated with neonatal levels of, for example, autoantibodies. This offers the potential to more reliably predict the likelihood on onset of Ti D compared to prior art methods.</p>
<p>In addition, the speed and simplicity of the methods described herein mean that it is possible to complete the methods of the invention in the period while the subject remains a neonate. This offers the benefit that where a likelihood of onset of Ti 0 is predicted, possible intervention therapies can be begun soon after birth (e.g. while the subject is still a neonate), which may maximise the chance of attaining an effective treatment that prevents onset of Ti D later in life.</p>
<p>Hence, in accordance with a second aspect of the invention, there is provided a method for identifying a neonatal subject for enrolment onto a potential prophylactic treatment program for the prevention of Ti D, which comprises performing the steps of any method of the invention so as to identify a neonate having a likelihood of onset of Ti 0 and enrolling said neonate into a prophylactic treatment program.</p>
<p>Once a subject has been identified who has a likelihood of developing Ti 0, it is advantageous to begin preventative (i.e. prophylactic) therapies as soon as possible. This is particularly true in cases where the subject is a neonate.</p>
<p>In this regard, during the first few days of life a normal process of thymic selection eliminates virtually all potentially dangerous cytotoxic 1-cells that may otherwise attack self-antigens. This process occurs as animportant and necessary part of the transition from a baby that is physically connected to its mother (via the placenta), to a neonate that must become self-sufficient.</p>
<p>Accordingly, T-cells that are autoreactive to insulin or pancreatic beta cells are typically eliminated in the thymus of normal individuals.</p>
<p>Without being bound by theory, it is thought that circulating amino acids and carnitine may influence the selection, development or destruction of Ti D-relevant cytotoxic T-cells. It is hypothesised that low levels of amino acids and carnitine may interfere with the thymic selection process and therefore, dangerous lymphocytes escape the thymic selection, enter the subject's circulating blood and go to populate the peripheral lymphoid tissue, such as lymph nodes. The presence of such autoreactive 1-cells represents a risk that that individual will develop Ti D (as discussed above). Even then, it is possible that other environmental factors (e.g. viruses, dietary factors etc.) can additionally trigger the progression to the diseased state.</p>
<p>By initiating a T1D prophylactic treatment as early as possible in the subject's life, and particularly during the first 3 days of life, the prospects of eliminating the risk of developing Ti D later in life will be increased.</p>
<p>Thus, in accordance with a third aspect of the invention, there is provided a method for the prophylactic treatment for Ti D, which comprises the steps of: (a) performing the steps of any of the diagnostic methods of the invention; (b) identifying one or more subject having a likelihood of onset of liD; and (c) prescribing to said one or more subject a prophylactic treatment for preventing or delaying the onset of Ti D. Optionally, the prophylactic treatment in step (c) comprises insulin replacement therapy.</p>
<p>In addition or in the alternative, the prophylactic treatment in step (c) may comprise immunomodulation therapy. Preferably, the immunomodulation therapy comprises anti-CD3 treatment and photopheresis.</p>
<p>In particular, it would be beneficial to influence the natural process of thymic selection in a neonate, such that a greater proportion (or all) of the auto-reactive T-cells involved in the development of Ti D are destroyed before disease progression is enabled. In this way, progression to the Ti D disease state may be delayed or even prevented.</p>
<p>In this regard, again without being bound by theory, it is thought that by administering appropriate amount of amino acids, amino acid derivatives, amino acid metabolites, and/or carnitine, to neonates-having relatively low circulating levels of one or more of these molecules (i.e. low levels in view of the subject's HLA genotype risk category), then the process of thymic selection may be returned to a stringency that is sufficient to eliminate Ti D-relevant auto-reactive 1-cells.</p>
<p>Thus, in a particularly preferred embodiment of the third aspect of the invention, the prophylactic treatment in step (c) comprises a dietary supplement. More preferably, the dietary supplement comprises an amino acid, amino acid derivative or amino acid metabolite supplement. In addition or in the alternative, the dietary supplement comprises a carnitine or acylcarnitine supplement.</p>
<p>Accordingly, in a fourth aspect of the invention there is provided a method of treating a neonate identified as having a predisposition to Ti D, comprising: administering a prophylactic composition that comprises one or more amino acid, amino acid derivative or amino acid metabolite in an amount sufficient to increase blood amino acid, amino acid derivative or amino acid metabolite levels to within the normal range.</p>
<p>Preferably, in the methods of the fourth aspect of the invention, the amino acid, amino acid derivative or amino acid metabolite is selected from the group consisting of: alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalanine, proline, tyrosine and valine.</p>
<p>More preferably, the prophylactic composition comprises all of alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalanine, proline, tyrosine and valine, and is administered in amounts sufficient to return / restore total blood amino acid, amino acid derivative or amino acid metabolite levels to within the normal range.</p>
<p>The prophylactic composition may comprise free carnitine and/or acylcamitine instead of the above-listed 15 amino acids. However, in a preferred embodiment, the prophylactic composition further comprises (i.e. in addition to amino acids, derivatives and metabolites) free carnitine and/or acylcarnitine and said administering comprises administering free camitine and/or acylcarnitine in amounts sufficient to return / restore free carnitine, acylcarnitine or total carnitine levels to within the normal range.</p>
<p>As the person of skill in the art will appreciate, the prophylactic compositions of the invention may be administered by any suitable means. In one embodiment, the prophylactic composition is administered orally.</p>
<p>Advantageously, the means of administration is suitable for neonates. In a preferred embodiment, therefore, the composition is administered parenterally, most preferably intravenously. Typically, said administering is in the form of a bolus injection, and still more preferably the composition is formulated as a slow release composition.</p>
<p>The time period over which the prophylactic treatment is administered may vary greatly depending on the type of treatment selected, or on the likely timescale of development of Ti D. For example,, it may be necessary to administer the treatment for the life of the.. subject, or until suitable follow-up tests demonstrate that the subject no longer has a likelihood of developing Ti D. Alternatively, where a particular risk time-period can be identified, it may be sufficient to cease treatment at the end of that period. For instance, In the case of a predisposition to early-onset Ti D (i.e. Ti D that develops within the first 4 years of a person's life), in may be sufficient to cease treatment at the end of this 4 year period.</p>
<p>The skilled person in the art can determine such periods by carrying out tests for Ti D indicators at relevant intervals. For example, the skilled person could</p>
<p>S</p>
<p>repeat the diagnostic methods of the invention at repeated intervals during the individual's life, or simply measure amino acid levels at repeated intervals.</p>
<p>In this regard, the above timeframes of treatment may advantageously be followed for treatments such as immunomodulation therapy and/or insulin replacement therapy and/or photopheresis.</p>
<p>In the case of a neonate who has been identified as having a likelihood of developing Ti D, however, the prophylactic treatment is preferably administered for at least the period during which the subject is classified a neonate (i.e. up to 3 days old). The period of time during which the prophylactic treatment / composition is administered may therefore vary according to when the method of the invention is carried out to predict the likelihood of onset of Ti D. Thus, where the subject is a neonate the prophylactic treatment / composition is preferably administered for at least 24 hours, at least 48 hours or at least 72 hours. Advantageously, the prophylactic treatment / composition is administered for less than 4 years, less than 3 years, less than 2 years or less than 1 year. Preferably, the prophylactic treatment / composition is administered until the subject is at least 3 days old (i.e. during the neonatal period of life).</p>
<p>Advantageously, during the treatment period (e.g. during the neonatal period), the prophylactic treatment is continuously effective.</p>
<p>The preferred form of prophylactic composition for use with neonatal subjects is a dietary supplement, and more preferably, a composition comprising one or more amino acid, amino acid derivative or amino acid metabolite and/or said free carnitine and/or acylcarnitine.</p>
<p>Hence, in a preferred embodiment, there is provided a method according to the fourth aspect of the invention, wherein the prophylactic composition is administered in an amount sufficient to restore to within the normal range the blood level of said one or more amino acid, amino acid derivative or amino acid metaboljte and/or said free carnitine and/or acylcarnitine and maintain said normal range for the duration of the neonatal period of the subject.</p>
<p>Since, each individual / subject may develop at a slightly different rate, it is convenient to sometime continue the prophylactic treatment slightly beyond the defined "neonatal period". Therefore, preferably, the above duration is at least 48 hours, more preferably at least 60 hours, and still more preferably at least 72 hours.</p>
<p>All references cited herein are incorporated by reference in their entirety.</p>
<p>Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.</p>
<p>The invention is further illustrated by the accompanying drawings in which: Figure 1 is a graph showing the relationship between the total concentration of amino acids in blood samples taken from Ti 0 patients and non-diabetic negative controls, in comparison to their HLA genotype risk category. The Medium/moderate and low HLA risk categories are grouped together. The boxes indicate the 75th percentile (top of box) and the 25 percentile (bottom of box).</p>
<p>Figure 2 is a graph showing the relationship between the total concentration of carnitine and acylcarnitine in blood samples taken from T1D patients and non-diabetic negative controls, in comparison to their HLA genotype risk category. Medium/moderate and low HLA risk categories are grouped together. The boxes indicate the 75th percentile (top of box) and the 25th percentile (bottom of box).</p>
<p>Figure 3 is a graph showing the relationship between the total concentration of amino acids in blood samples taken from Ti D patients and non-diabetic negative controls, in comparison to their HLA genotype risk category, plotted in the 95% confidence interval.</p>
<p>Medium/moderate and low HLA risk categories are grouped together.</p>
<p>Figure 4 is a graph showing the relationship between the total concentration of carnitine and acylcarnitine in blood samples taken from Ti D patients and non-diabetic negative controls, in comparison to their HLA genotype risk category, plotted in the 95% confidence interval. Medium/moderate and low HLA risk categories are grouped together.</p>
<p>Example</p>
<p>(I) Methods A prospective study was conducted to determine the relevance of concentration of circulating amino acids, amino acid derivatives, amino acid metabolites, free camitine and acylcarnitine to the future development of type 1 diabetes (Ti D).</p>
<p>Diabetic patients were identified from within the Unit of Diabetology at the Bambino Gesü Paediatric Hospital in Rome. Controls were selected from 1650 children, who were HLA typed at birth for genetic susceptibility to develop Ti D (Buzzetti R. et a!. 2004, Diabetes Metab Res Rev. 20(2): 137-43).</p>
<p>As a criteria of inclusion, children had be born in Lazio between January 2000 and December 2002 and Ti D had to be developed by the age of 4 years.</p>
<p>These criteria were adopted in order to: 1) have the possibility of retrieving dry blood spot samples; 2) reduce the time elapsing between birth and conducting the assays; and 3) identify controls matched for age, sex and genetic HLA risk categories (i.e. high, moderate/medium and low), defined as previously described in Buzzetti R. et a!. 2004, supra.</p>
<p>Overall, 11 diabetic children fulfilled the selection criteria and 44 matched controls were identified. These ii diabetic children represent nearly all of the children who have developed Ti D in the first 4 years of life from a sample size of 150,000 children born in the Lazio region over the relevant time period.</p>
<p>Information regarding the subjects used in the study is provided in Table 1.</p>
<p>Informed consent was obtained from parents of all children.</p>
<p>Information regarding birth weight, gestational age at birth and feeding in the first week of life was recorded.</p>
<p>Blood dry spots were retrieved from the two centers in charge of neonatal screenings (e.g. congenital hypothyroidism and phenylketonuria) in the same region. All spots were collected within the first days after birth (i.e. during the neonatal period) and stored at room temperature until the analysis. Once samples had been identified, they were coded and no information regarding Ti 0 status was available before the results of the study had been obtained.</p>
<p>For each blood spot, concentrations of 15 amino acids (in 13 categories), along with the concentrations of total carnitine (TO), free carnitine (FC) and acylcarnitine (AC) were measured and recorded (see Table 2). Internal AC and amino acid standards were provided by Cambridge Isotopes Laboratories, Andover, MA, USA.</p>
<p>I</p>
<p>Carnitine and amino acids were analyzed on filter paper (W903) blood spots as butyl esters. The butylated samples were tested with a tandem mass spectrometer (Sciex API 365, PE Sciex Instrument, Concord, ON, Canada).</p>
<p>This mass spectrophotometer is routinely used for neonatal mass screening.</p>
<p>Quality control of the methods for measuring amino acids, camitine and acylcarnitine was performed by Dr Piero Rinaldo, (Mayo Clinic, Ronchester, MN, USA).</p>
<p>TC, FC, AC and AC/FC ratio were analyzed. Amino acids were also studied as the algebraic sum of essential, non-essential and total amino acid concentrations. The Mann-Whitney U-Test was used for comparison of continuous variables. Frequencies of qualitative variables were evaluated using Fisher's exact test. A two-tailed P value of less than 0.05 was considered to be significant. All analyses were performed using SPSS 11.5.1 for Windows Package (LEAD Technologies, mc).</p>
<p>(ii) Results Referring to Table 1, among the 11 diabetic children, 4 were found to possess the high risk HLA genotype for developing Ti D; 6 were found to have the medium/moderate risk HLA genotypes; and 1 had a low risk HLA genotype.</p>
<p>The median age at the onset of Ti D was 2.7 years, and onset age ranged from 1.1 to 3.8 years.</p>
<p>No differences were seen in reference to gestational age at birth, birth weight and type of feeding in the first week of life (P=0.438, P=0.408 and P=0.522, respectively). Thus, in this sample it did not appear that feeding on formula milk or breast milk was relevant.</p>
<p>The time elapsing from the collection of blood spots to the performance of the assays was comparable in the two groups (P=O. 191), SO it is considered that any slight differences were insignificant.</p>
<p>Turning to Table 2 and Figures 1 and 3, it can be seen that the diabetic patients displayed lower levels of all of the measured amino acids than did the non-diabetic controls. Moreover, the concentrations of the amino acids Ala, Gly, Glu/GIn, Leu/lle, Orn, Phe and Pro were seen to be significantly lower in diabetic subjects than in the non-diabetic control subjects. Overall, total amino acid concentrations were significantly lower in diabetic patients than in controls, as were the levels of essential amino acids and non-essential amino acids. Although the concentration of the essential amino acids shown in Table 2 does not include that of arginine (which is essential in neonates), if arginine is included in the "essential" amino acid subgroup, rather than in the iS "non-essential" subgroup, the above observation regarding the relative levels of amino acids remains true.</p>
<p>Figure 1 shows the total amino acid concentrations measured in blood samples of either Ti D patients or non-diabetic controls, depending on their HLA genotype risk category. The boxes indicate the 25th to the 75th percentile. In addition to the lower relative concentration of amino acids found in the samples of Ti D patients, the range of total amino acid concentrations measured appears to be narrower in the Ti D patients.</p>
<p>Figure 3 shows the 95% confidence interval (95% Cl) of the total amino acid concentrations measured in blood samples of either Ti D patients or non-diabetic controls, displayed according to the subject's HLA genotype risk category. Again, it can be seen that the amino acid concentrations are significantly lower in the Ti D patients than in the non-diabetic controls.</p>
<p>Also from Table 2 and Figures 2 and 4, it can be seen that circulating TC, FC and AC concentrations were significantly lower in diabetic patients compared to the non-diabetic controls. However, essentially no differences were detected in the AC/FC ratio.</p>
<p>Figure 2 shows the total carnitine concentrations measured in blood samples of either Ti D patients or non-diabetic controls, depending on their HLA genotype risk category. The boxes indicate the 25th to the 75th percentile.</p>
<p>Again, in addition to the lower relative amount of carnitine found in the samples of Ti D patients, the range of total carnitine concentrations measured appears to be narrower in the Ti D patients.</p>
<p>Figure 4 shows the 95% confidence interval (95% Cl) of the total carnitine concentrations measured in blood samples of either Ti 0 patients or non-diabetic controls, displayed according to the subject's HLA genotype risk category. Again, it can be seen that the carnitine concentrations are significantly lower in the Ti 0 patients than in the non-diabetic controls.</p>
<p>The data used in Figures 1 and 2 is displayed in Table 3 and the data used in Figures 3 and 4 is displayed in Table 4.</p>
<p>(iii) Conclusions</p>
<p>This is the first demonstration that neonates who go on to develop Ti D early in life (i.e. in the first 4 years) display reduced concentrations of amino acids and of carnitine during the neonatal period.</p>
<p>Since this investigation was carried out retrospectively on dried blood spots stored at room temperature, as a control, 80 dried blood spots taken from children of the same region, and which have undergone similar storage conditions in the same centers for two and four years, respectively, have been tested using the same tandem mass spectrometer used for the Example.</p>
<p>Values of amino acids and of TO, FC and AC, were measured and found to be very similar in the two sample sets (inventors' unpublished results). In the Example described, since the time elapsing between blood collection and the conduction of the assays was similar between diabetics and controls, and was carried out in the time range of 2-4 years, the differences in amino acid and carnitine concentrations noted between diabetics and non-diabetic controls can be considered not to be due to the latency between blood collection and sample testing.</p>
<p>For the reasons already discussed with regard to Table 1, it is also possible to exclude the differences in the type of feeding, because the proportion of children whom were breast-fed or whom were fed formula milk was comparable between the two groups.</p>
<p>It is therefore concluded that children who later developed T1D early in life have reduced levels of circulating amino acids and carnitine at birth or soon after birth (i.e. during the neonatal period). Thus, low levels of amino acids and of camitine may be viewed as additional markers for predicting the future onset of Ti D in children, irrespective of their perceived genetic susceptibility.</p>
<p>The determination of these compounds is easy to do and can be routinely applied in population screenings. Hence, new perspectives for the testing of all newborn children, to predict the likely onset of Ti D, and if necessary commence a suitable prophylactic treatment regime are apparent.</p>
<p>Table 1. Characteristics of diabetic children and matched controls Diabetic Control Significance Patients children P (N=11) (N=44) M:F 7:4 28:16 -HLA risk category -high 4 16 -moderate 6 24 --low 1 4 Gestational age at birth 39 (37-41) 39 (30-41) 0.438 (weeks) Birth weight (Kg) 3.5 (3.0-4.1) 3.4 (1.6-4.8) 0.408 Feeding during the l week 6 26 0.522 -only breast milk 5 18 -formula or mixed Age at onset of T1D (yrs) 2.7 (1.1-3.8) --Age at metabolic 3.9 (2.5-5.0) 3.2 (1.6-4.9) 0.191 evaluation (yrs) Values are expressed as number of cases or as median (range).</p>
<p>Table 2. Carnitine and amino acid concentrations (jtmol/L) in blood dry spots collected within the first three days of life Diabetic Patients Control children Significance (N=11) (N=44) P Total carnitine 29.9 (14.8-38.9) 39.5 (19.7-96.1) 0.004 Free carnitine 19.0 (6.8-25.6) 24.7(11.7-68.9) 0.009 Acylcarnitine 10.7 (7.8-16.3) 16.5 (8.0-27.2) 0.009 AcylIfree ratio 0.7 (0.4-1.2) 0.6 (0.3-1.2) 0.556 Alanine (Ala) 82.5 (50.7-174.4) 140.8 (50.1-526.7) 0.037 Arginine (Arg) 15.1 (10-34.6) 17.4 (7.3-49.0) 0.599 Aspartate (Asp) 35.9 (15.8-70.4) 42.9 (18.0-1 52.9) 0. 461 Citrulline (Cit) 4.6 (2.9-13.3) 6.8 (2.4-22.1) 0.064 Glycine (Gly) 80.9 (49.8-131.2) 122.1 (66.0-354-7) 0.002 Glutammate/Glutammine (Glu/GIn) 223.8 (114.4-332.1) 314.0 (211.2-578.1) 0.002 *Leucine/lsoleucine (Leu/Xle) 60.9 (42.2-84.6) 86.9 (51.5-191.2) <0.001 *Methionine (Met) 3.6 (1.9.8.2) 4.4 (1.3-10.9) 0.326 Ornitine (Orn) 6.7 (4.0-16.8) 11.0 (3.4-34.8) 0.001 *phenylalanine (Phe) 15.2 (3.9-29.8) 24.9 (12.4-54.8) 0.001 Proline (Pro) 45.3 (24.4-73.7) 73.9 (34.2-128.5) 0.002 Thyrosine (Thy) 26.4 (14.1-65.2) 32.3 (13.7-80.3) 0.323 *Valine (Val) 59.8 (29.8-1 50) 67.8 (10-250) 0.192 Essential aminoacids 134 (91 -242) 177 (120-426) 0.003 Non essential aminoacids 549 (424-852) 781 (465-1805) 0.003 Total aminoacids 677 (515-1016) 954 (618-2230) 0.003 Values are expressed as median (range) * Essential amino acids (excluding arginine)</p>
<p>S</p>
<p>Table 3. Total amino acid and carnitine concentrations measured in samples taken from subjects with either the high risk HLA genotype or with the moderate or low risk HLA genotypes (as plotted in Figures 1 and 2 respectively) high risk high risk moderate or low risk moderate or low risk diabetics controls diabetics controls (Nr=4) (Nr=16) (Nr=7) (Nr=28) Total amino acids (molIL) maximum 1016.4 2231 909.6 1628.3 75th percentile 996.4 1543 677.2 1191.7 median 836.8 1229.6 673.3 849 25th percentile 735.8 960.5 520.5 715.9 minimum 735.2 618.5 515.6 632.2 Total carnitine (pmollL) maximum 38.9 69.6 31.8 96.1 75thpercentile 38.4 57.1 31.78 44.8 median 35.4 49.2 26.4 37.8 25th percentile 30.7 31.8 18.9 30.2 minimum 29.7 19.7 14.8 25.3</p>
<p>I</p>
<p>Table 4. Total amino acid and carnitine concentrations measured in samples taken from subjects with either the high risk HLA genotype or with the moderate or low risk HLA genotypes (as plotted in Figures 3 and 4 respectively) high risk high risk moderate or low risk moderate or low risk diabetics controls diabetics controls (Nr = 4) (Nr = 16) (Nr = 7) (Nr = 28) Total amino acids (pmol/L) 95%CI upper limit 1082.9 1493.4 774.5 1039.7 95%Cl lower limit 629.8 1035.6 523.2 833.2 95%Cl midpoint 856.3 1264.5 648.8 936.5 Total carnitine (pmollL) 95%Cl upper limit 41.3 53.2 31.3 46.9 95%Cl lower limit 28.4 37.1 19.1 35.1 95%Cl midpoint 34.9 45.1 25.2 41</p>
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<p>Virtanen SM, Knip M., Nutritional risk predictors of beta cell autoimmunity and type 1 diabetes at a young age. (2003) Am. J. C/in. Nutr., Dec; 78(6): 1053-67.</p>
<p>Ziegler AG, Schmid S, Huber D, Hummel M, Bonifacio E., Early infant feeding and risk of developing type 1 diabetes-associated autoantibodies. (2003) J. Am. Med. Assoc., Oct 1; 290(1 3): 1721-8.</p>
<p>Redondo MJ, Fain PR, Eisenbarth GS. Genetics of type 1A diabetes.(2001) Recent Prog. Horm. Res. 56: 69-89.</p>
<p>llonen J, Sjoroos M, Knip M, Veijola R, Simell 0, Akerblom HK, Paschou P, Bozas E, Havarani B, Malamitsi-Puchner A, Thymelli J, Vazeou A, Bartsocas CS. (2002) Estimation of genetic risk for type 1 diabetes. Am. J. Med. Genet.</p>
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<p>Mimbacas A, Perez-Bravo F, Hidalgo PC, JavielG, Pisciottano..C, Grignola A, Jorge AM, Gallino JP, Gasagoite J, Cardoso H. (2003) Association between diabetes type 1 and DQB1 alleles in a case-control study conducted in Montevideo, Uruguay. Genet. Mo!. Res. 2(1): 29-35.</p>
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<p>(2004), Diabetes Metab. Res. Rev., Mar-Apr; 20(2): 137-43.</p>
<p>Galgani A, Petrone A, Spoletini M, Hodge A, Del Buono ML, Locatelli M, Buzzetti R and the Diabfin Study Group. HLA class II typing in newborns reveals a low frequency of the DRB1*04 allele and a high frequency of DRB1 *11 allele in three regions of continental Italy. (2004) Hum. Immunol. 65(4): 366-72.</p>
Claims (1)
- <p>S</p><p>CLAIMS: 1. An in vitro method for predicting the onset of type 1 diabetes (Ti D) in a subject, comprising the steps of: (a) measuring the concentration of at least one amino acid, amino acid derivative or amino acid metabolite in a biological sample taken from the subject; (b) determining the subject's HLA genotype; (c) assigning the subject's genetic risk of developing Ti D on io the basis of the subject's HLA genotype; (d) combining the information obtained in step (a) with the information in step (C); and (e) predicting the likelihood of onset of Ti D based upon the combination of step (d).</p><p>2. The method of Claim 1, wherein in step (c) the genetic risk is assigned as "high", "moderate" or "low" according to the subject's HLA genotype.</p><p>3. The method of Claim 2, wherein a high risk HLA genotype comprises the genetic markers: DRB1 *03 / *04 (not 0403), DQB1 0302.</p><p>4. The method of Claim 2, wherein a moderate/medium risk HLA genotype comprises the genetic markers: DRB1 *04 (not 0403) / *04 (not 0403), DQB1 0302; DRB1*04 (not 0403)/ X, DQB1 0302 /DQB1 not 0602-3) DRB1 *03 / *03; DRB1 *03 / X, DQB1 not 0602-3, not 0301, not 0503; wherein X is not DRB1*03, *04, or 0403.</p><p>5. The method of Claim 2, wherein a low risk HLA genotype comprises any combination of genetic markers not specified in Claim 3 or Claim 4.</p><p>S</p><p>6. The method of any preceding claim, wherein the amino acid, amino acid derivative or amino acid metabolite is selected from the group consisting of: alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalanine, proline, tyrosine and valine.</p><p>7. The method of any preceding claim, wherein in step (a) the concentration of two or more amino acids, amino acid derivatives or amino acid metabolites are measured.</p><p>8. The method of any preceding claim, wherein in step (a) the concentration of essential amino acids is measured.</p><p>9. The method of any of Claims 1 to 7, wherein in step (a) the concentration of non-essential amino acids is measured.</p><p>10. The method of Claim 6, wherein in step (a) the total concentration of all of the amino acids, amino acid derivatives and amino acid metabolites is measured.</p><p>11. The method of Claim 10, wherein in step (e) a total amino acid concentration of less than approximately 1200 limol/L in combination with a high risk HLA genotype is indicative of the likely future onset of T1D.</p><p>12. The method of Claim 11, wherein a total amino acid concentration of approximately 500-1200 t.tmol/L is indicative of the likely future onset of T1D.</p><p>13. The method of Claim 10, wherein in step (e) a total amino acid concentration of less than approximately 1000 imol/L in combination</p><p>S</p><p>with a moderate or low risk HLA genotype is indicative of the likely future onset of Ti 0.</p><p>14. The method of Claim 13, wherein a total amino acid concentration of approximately 300-1000 imol/L is indicative of the likely future onset of T1D.</p><p>15. The method of any preceding claim, additionally comprising the steps of: (a') measuring the concentration of at least one of: free carnitine, acylcarnitine and total carnitine in a sample taken from said subject; and (d') combining the information obtained in step (a') with the information in step (c); and (e') predicting the likelihood of onset of Ti D based upon the combination of step (d) and the combination of step (d').</p><p>16. The method of Claim 15, wherein in step (a') the concentrations of free carnitine, acylcamitine and total camitine are measured.</p><p>17. The method of Claim 15 or Claim 16, wherein in step (e') a total carnitine concentration of less than approximately 50 I.tmol/L in combination with a high risk HLA genotype is indicative of the likely future onset of Ti D. 18. The method of Claim 17, wherein a total carnitine concentration of approximately 20-50 i.tmol/L is indicative of the likely future onset of liD.</p><p>19. The method of Claim 15 or Claim 16, wherein in step (e') a total carnitine concentration of less than approximately 40 lAmol/L in combination with a moderate or low risk HLA genotype is indicative of the likely future onset of Ti D. 20. The method of Claim 19, wherein a total carnitine concentration of approximately 10-40 i.imol/L is indicative of the likely future onset of T1D.</p><p>21. The method of any preceding claim, wherein in step (a) said biological sample is a blood sample.</p><p>22. The method of Claim 21, wherein said blood sample is collected within 3 days (i.e. 72 hours) of the subject's birth.</p><p>23. The method of any preceding claim, wherein step (b) is carried out by genetically screening a portion of said sample used in step (a).</p><p>24. The method of any of Claims 15 to 23, wherein step (a') is carried out on the sample used in step (a).</p><p>25. The method of any preceding claim, wherein the subject is a neonate.</p><p>26. A method for identifying a neonatal subject for enrolment onto a potential prophylactic treatment program for Ti D, which comprises performing the steps of Claim 25 to identify a neonate having a likelihood of onset of Ti D and enrolling said neonate into a prophylactic treatment program.</p><p>27. A method for the prophylactic treatment for Ti D, which comprises the steps of: (a) performing the steps of any of Claims 1 to 25; (b) identifying one or more subject having a likelihood of onset of Ti D; and (c) prescribing to said one or more subject a prophylactic treatment for preventing or delaying the onset of Ti D. 28. The method of Claim 27, wherein said prophylactic treatment in step (c) comprises insulin replacement therapy.</p><p>29. The method of Claim 27 or Claim 28, wherein said prophylactic treatment in step (c) comprises immunomodulation therapy.</p><p>30. The method of Claim 29, wherein the immunomodulation therapy comprises anti-CD3 treatment.</p><p>31. The method of Claim 27 or Claim 28, wherein said prophylactic treatment in step (c) comprises photopheresis.</p><p>32. The method of any of Claims 27 to 31, wherein said prophylactic treatment in step (c) comprises a dietary supplement.</p><p>33. The method of Claim 32, wherein said dietary supplement comprises an amino acid, amino acid derivative or amino acid metabolite supplement.</p><p>34. The method of Claim 32 or Claim 33, wherein said dietary supplement comprises a camitine or acylcarnitine supplement.</p><p>35. A method of treating a neonate identified as having a predisposition to T1D, comprising: administering a prophylactic composition that comprises one or more amino acid, amino acid derivative or amino acid metabolite in an amount sufficient to increase blood amino acid, amino acid derivative or amino acid metabolite levels to within the normal range.</p><p>36. The method of Claim 35, wherein said amino acid, amino acid derivative or amino acid metabolite is selected from the group consisting of: alanine, arginine, aspartate, citrulline, glycine, glutamate, glutam in leucine, isoleucine, methionine, ornithine, phenylalanine, proline, tyrosine and valine.</p><p>37. The method of Claim 36, wherein said composition comprises all of alanine, arginine, aspartate, citrulline, glycine, glutamate, glutamine, leucine, isoleucine, methionine, ornithine, phenylalanine proline, tyrosine and valine, in amounts sufficient to restore total blood amino acid, amino acid derivative or amino acid metabolite levels to within the normal range.</p><p>38. The method of any of Claims 35 to 37, wherein said composition further comprises free carnitine and/or acytcamitine and said administering comprises administering free camitine and/or acylcarnitine in amounts sufficient to restore free carnitine, acylcarnitine or total carnitine levels to within the normal range.</p><p>39. The method of any of Claims 35 to 38, wherein said composition is administered parenterally.</p><p>40. The method of Claim 39, wherein said administering is in the form of a bolus injection.</p><p>41. The method of Claim 39 or Claim 40, wherein said composition is a slow release composition.</p><p>42. The method of any of Claims 35 to 38, wherein said composition is administered orally.</p><p>43. The method of any of Claims 35 to 42, wherein said composition is administered in an amount sufficient to restore to within the normal range the blood level of said one or more amino acid, amino acid derivative or amino acid metabolite and/or said free carnitine and/or acylcarnitine and maintain said normal range for a duration of at least 48 hours.</p><p>44. The method of Claim 43, wherein said duration is at least 72 hours.</p>
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GB0615063A GB2440554A (en) | 2006-07-28 | 2006-07-28 | Prediction of the onset of type 1 diabetes. |
CA2691147A CA2691147C (en) | 2006-07-28 | 2007-07-30 | Prediction and prophylactic treatment of type 1 diabetes |
US12/375,411 US8063011B2 (en) | 2006-07-28 | 2007-07-30 | Prediction and prophylactic treatment of type 1 diabetes |
AU2007298649A AU2007298649B2 (en) | 2006-07-28 | 2007-07-30 | Prediction and prophylactic treatment of type 1 diabetes |
EP07858847A EP2047278B1 (en) | 2006-07-28 | 2007-07-30 | Prediction of type 1 diabetes |
PCT/IB2007/003024 WO2008035204A2 (en) | 2006-07-28 | 2007-07-30 | Prediction and prophylactic treatment of type 1 diabetes |
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GB0615063A GB2440554A (en) | 2006-07-28 | 2006-07-28 | Prediction of the onset of type 1 diabetes. |
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Cited By (1)
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CN107121540A (en) * | 2016-02-24 | 2017-09-01 | 中国科学院上海生命科学研究院 | The application of fatty acyl carnitine spectrum or its detection reagent in the prediction of diabetes B onset risk |
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2006
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Non-Patent Citations (2)
Title |
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Diabetes Metab. Res., Vol.20, 2004, Buzzetti, R. et al., "Genetic prediction of type 1 diabetes...", pp.137-143 * |
Diabetologia, Vol.48 Suppl.1, 2005,Locatelli, M. et al., "Children who developtype 1 diabetes...", p.A 114, Abs No.302 * |
Cited By (1)
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CN107121540A (en) * | 2016-02-24 | 2017-09-01 | 中国科学院上海生命科学研究院 | The application of fatty acyl carnitine spectrum or its detection reagent in the prediction of diabetes B onset risk |
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