GB2399820A - Recovery of biofilament proteins from biological fluids - Google Patents
Recovery of biofilament proteins from biological fluids Download PDFInfo
- Publication number
- GB2399820A GB2399820A GB0416692A GB0416692A GB2399820A GB 2399820 A GB2399820 A GB 2399820A GB 0416692 A GB0416692 A GB 0416692A GB 0416692 A GB0416692 A GB 0416692A GB 2399820 A GB2399820 A GB 2399820A
- Authority
- GB
- United Kingdom
- Prior art keywords
- proteins
- recovery
- biological fluids
- biofilament
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to methods of purifying and recovering biofilament proteins, such as spider silk proteins, from complex biological solutions, including, for example, cell culture media, plant extract, milk, urine, saliva, sweat, tears, seminal fluid, and blood. The process comprises steps of concentration and purification. Additional clarification steps and polishing procedures may also be added. The process employs precipitation, filtration, centrifugation and chromatography methods.
Description
GB 2399820 A continuation (74) Agent and/or Address for Service: Marks &
Clerk 19 Royal Exchange Square, GLASGOW, G1 3AE, United Kingdom
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US34747102P | 2002-01-11 | 2002-01-11 | |
PCT/CA2003/000025 WO2003057720A2 (en) | 2002-01-11 | 2003-01-13 | Recovery of biofilament proteins from biological fluids |
Publications (2)
Publication Number | Publication Date |
---|---|
GB0416692D0 GB0416692D0 (en) | 2004-09-01 |
GB2399820A true GB2399820A (en) | 2004-09-29 |
Family
ID=23363832
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB0416692A Withdrawn GB2399820A (en) | 2002-01-11 | 2003-01-13 | Recovery of biofilament proteins from biological fluids |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2003202330A1 (en) |
GB (1) | GB2399820A (en) |
WO (1) | WO2003057720A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050161058A1 (en) * | 2004-01-26 | 2005-07-28 | Israel Yerushalmy | Spider silk dental floss |
CA2635660C (en) | 2005-12-30 | 2016-02-23 | Spiber Technologies Ab | Spider silk proteins and methods for producing spider silk proteins |
JP6081908B2 (en) | 2010-03-31 | 2017-02-15 | アーエムシルク ゲーエムベーハー | Separation of insoluble target proteins |
FR2958496A1 (en) * | 2010-04-09 | 2011-10-14 | Bioprotein Technologies Sa | PREPARATION OF RECOMBINANT HUMAN PHOSPHOLIPID PHOSPHOLIPID (PLTP) PLASMA PROTEIN FROM MILK OF TRANSGENIC ANIMALS. |
CN102167724B (en) * | 2011-01-04 | 2013-10-16 | 复旦大学 | Controllable preparation method for high-concentration regenerated silk protein aqueous solution |
WO2017106631A1 (en) * | 2015-12-18 | 2017-06-22 | Tufts University | Silk solution purification system, concentrating system, and methods thereof |
EP3263593A1 (en) | 2016-07-01 | 2018-01-03 | Anna Rising | Engineered spider silk proteins and uses thereof |
CN112566767A (en) * | 2018-08-10 | 2021-03-26 | 保尔特纺织品公司 | Composition for molded bodies |
CN109520785B (en) * | 2018-11-09 | 2021-06-04 | 董艳 | A middle section urine collection system for nephrology dept |
JP2022513628A (en) * | 2018-11-28 | 2022-02-09 | ボルト スレッズ インコーポレイテッド | Alkaline purification method for spider silk protein |
WO2022177949A1 (en) * | 2021-02-16 | 2022-08-25 | Connor Alexander Joseph | Upcycling of plastic waste to recombinant silk proteins via pseudomonas bacteria |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5156956A (en) * | 1987-03-04 | 1992-10-20 | Ajinomoto Co., Inc. | Transgultaminase |
WO1997008315A1 (en) * | 1995-08-22 | 1997-03-06 | Basel Richard M | Cloning methods for high strength spider silk proteins |
WO1999047661A2 (en) * | 1998-03-17 | 1999-09-23 | Nexia Biotechnologies, Inc. | Production of biofilaments in transgenic animals |
US5989894A (en) * | 1990-04-20 | 1999-11-23 | University Of Wyoming | Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA |
DE10113781A1 (en) * | 2000-06-09 | 2001-12-13 | Ipk Inst Fuer Pflanzengenetik | New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins |
-
2003
- 2003-01-13 AU AU2003202330A patent/AU2003202330A1/en not_active Abandoned
- 2003-01-13 GB GB0416692A patent/GB2399820A/en not_active Withdrawn
- 2003-01-13 WO PCT/CA2003/000025 patent/WO2003057720A2/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5156956A (en) * | 1987-03-04 | 1992-10-20 | Ajinomoto Co., Inc. | Transgultaminase |
US5989894A (en) * | 1990-04-20 | 1999-11-23 | University Of Wyoming | Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA |
WO1997008315A1 (en) * | 1995-08-22 | 1997-03-06 | Basel Richard M | Cloning methods for high strength spider silk proteins |
WO1999047661A2 (en) * | 1998-03-17 | 1999-09-23 | Nexia Biotechnologies, Inc. | Production of biofilaments in transgenic animals |
DE10113781A1 (en) * | 2000-06-09 | 2001-12-13 | Ipk Inst Fuer Pflanzengenetik | New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins |
Non-Patent Citations (2)
Title |
---|
Nature Biotechnology 2001, 19(6), pp.573-577 - Scheller et al. * |
Science 2002, 295(5554), pp.472-476 - Lazaris et al. * |
Also Published As
Publication number | Publication date |
---|---|
GB0416692D0 (en) | 2004-09-01 |
WO2003057720A2 (en) | 2003-07-17 |
WO2003057720A3 (en) | 2004-03-04 |
AU2003202330A1 (en) | 2003-07-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB2399820A (en) | Recovery of biofilament proteins from biological fluids | |
US7329520B2 (en) | Fructosyl peptide oxidase and utilization thereof | |
DE60009912D1 (en) | METHOD FOR RNASE- AND ORGANIC SOLVENT-FREE PURIFICATION OF PLASMID DNA BY TANGENTIAL FLOW FILTRATION | |
Ware et al. | Evaluation of an alternative IMS dissociation procedure for use with Method 1622: detection of Cryptosporidium in water | |
ATE403671T1 (en) | METHODS FOR THE PURIFICATION OF RECOMBINATE HUMAN ERYTHROPOIETIN FROM CELL CULTURE SUPERNATIVES | |
WO2007031949A3 (en) | Liquid media for chlamydospore production of the fungus pochonia chlamydosporia | |
WO2005030040A8 (en) | Hematopoietic stem cell identification and isolation | |
JPH08228795A (en) | Preparation of sample for analyzing saccharide chain and diagnosis using the same sample | |
Roboz et al. | Elimination of 2-deoxyribose interference in the thiobarbituric acid determination of N-acetylneuraminic acid in tumor cells by pH-dependent extraction with cyclohexanone | |
EP1132483A3 (en) | Method for diagnosing schizophrenia using objective indices | |
JPH02255686A (en) | Production of ellagic acid | |
EP1500430A3 (en) | Separating agent for optical isomers, method of production thereof, and separation column for optical isomers | |
JPH04504665A (en) | Nitrate reductase from yeast, its preparation and use | |
US6773913B2 (en) | Device and process for purifying vectors | |
Walker et al. | The Language of Biotechnology: A Dictionary of Terms. | |
AU4064901A (en) | Detection of human papilloma viruses | |
JP2006515568A (en) | Method for purifying recombinant protein from complex media and purified protein obtained thereby | |
WO2003072807A3 (en) | Sterility testing apparatus | |
CN102209793B (en) | Nucleotide sequences, methods and kits for detecting HPV | |
Swanson et al. | Dipeptidyl peptidase III of human cataractous lenses. Partial purification | |
JP2598676B2 (en) | Protease production method | |
EP1930419A1 (en) | Method for production of enzyme | |
CA2126090C (en) | Process for preparing neuraminidase | |
SU1325076A1 (en) | Method of recovering and purifying urokinase from biological fluids | |
GB2418668A (en) | Anti-inflammatory assay and compounds |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |