GB2399820A - Recovery of biofilament proteins from biological fluids - Google Patents

Recovery of biofilament proteins from biological fluids Download PDF

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Publication number
GB2399820A
GB2399820A GB0416692A GB0416692A GB2399820A GB 2399820 A GB2399820 A GB 2399820A GB 0416692 A GB0416692 A GB 0416692A GB 0416692 A GB0416692 A GB 0416692A GB 2399820 A GB2399820 A GB 2399820A
Authority
GB
United Kingdom
Prior art keywords
proteins
recovery
biological fluids
biofilament
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0416692A
Other versions
GB0416692D0 (en
Inventor
Costas N Karatzas
Yue Huang
Carl Turcotte
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nexia Biotechnologies Inc
Original Assignee
Nexia Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nexia Biotechnologies Inc filed Critical Nexia Biotechnologies Inc
Publication of GB0416692D0 publication Critical patent/GB0416692D0/en
Publication of GB2399820A publication Critical patent/GB2399820A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to methods of purifying and recovering biofilament proteins, such as spider silk proteins, from complex biological solutions, including, for example, cell culture media, plant extract, milk, urine, saliva, sweat, tears, seminal fluid, and blood. The process comprises steps of concentration and purification. Additional clarification steps and polishing procedures may also be added. The process employs precipitation, filtration, centrifugation and chromatography methods.

Description

GB 2399820 A continuation (74) Agent and/or Address for Service: Marks &
Clerk 19 Royal Exchange Square, GLASGOW, G1 3AE, United Kingdom
GB0416692A 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids Withdrawn GB2399820A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34747102P 2002-01-11 2002-01-11
PCT/CA2003/000025 WO2003057720A2 (en) 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids

Publications (2)

Publication Number Publication Date
GB0416692D0 GB0416692D0 (en) 2004-09-01
GB2399820A true GB2399820A (en) 2004-09-29

Family

ID=23363832

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0416692A Withdrawn GB2399820A (en) 2002-01-11 2003-01-13 Recovery of biofilament proteins from biological fluids

Country Status (3)

Country Link
AU (1) AU2003202330A1 (en)
GB (1) GB2399820A (en)
WO (1) WO2003057720A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050161058A1 (en) * 2004-01-26 2005-07-28 Israel Yerushalmy Spider silk dental floss
CA2635660C (en) 2005-12-30 2016-02-23 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
JP6081908B2 (en) 2010-03-31 2017-02-15 アーエムシルク ゲーエムベーハー Separation of insoluble target proteins
FR2958496A1 (en) * 2010-04-09 2011-10-14 Bioprotein Technologies Sa PREPARATION OF RECOMBINANT HUMAN PHOSPHOLIPID PHOSPHOLIPID (PLTP) PLASMA PROTEIN FROM MILK OF TRANSGENIC ANIMALS.
CN102167724B (en) * 2011-01-04 2013-10-16 复旦大学 Controllable preparation method for high-concentration regenerated silk protein aqueous solution
WO2017106631A1 (en) * 2015-12-18 2017-06-22 Tufts University Silk solution purification system, concentrating system, and methods thereof
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
CN112566767A (en) * 2018-08-10 2021-03-26 保尔特纺织品公司 Composition for molded bodies
CN109520785B (en) * 2018-11-09 2021-06-04 董艳 A middle section urine collection system for nephrology dept
JP2022513628A (en) * 2018-11-28 2022-02-09 ボルト スレッズ インコーポレイテッド Alkaline purification method for spider silk protein
WO2022177949A1 (en) * 2021-02-16 2022-08-25 Connor Alexander Joseph Upcycling of plastic waste to recombinant silk proteins via pseudomonas bacteria

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156956A (en) * 1987-03-04 1992-10-20 Ajinomoto Co., Inc. Transgultaminase
WO1997008315A1 (en) * 1995-08-22 1997-03-06 Basel Richard M Cloning methods for high strength spider silk proteins
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
US5989894A (en) * 1990-04-20 1999-11-23 University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA
DE10113781A1 (en) * 2000-06-09 2001-12-13 Ipk Inst Fuer Pflanzengenetik New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5156956A (en) * 1987-03-04 1992-10-20 Ajinomoto Co., Inc. Transgultaminase
US5989894A (en) * 1990-04-20 1999-11-23 University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the DNA
WO1997008315A1 (en) * 1995-08-22 1997-03-06 Basel Richard M Cloning methods for high strength spider silk proteins
WO1999047661A2 (en) * 1998-03-17 1999-09-23 Nexia Biotechnologies, Inc. Production of biofilaments in transgenic animals
DE10113781A1 (en) * 2000-06-09 2001-12-13 Ipk Inst Fuer Pflanzengenetik New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nature Biotechnology 2001, 19(6), pp.573-577 - Scheller et al. *
Science 2002, 295(5554), pp.472-476 - Lazaris et al. *

Also Published As

Publication number Publication date
GB0416692D0 (en) 2004-09-01
WO2003057720A2 (en) 2003-07-17
WO2003057720A3 (en) 2004-03-04
AU2003202330A1 (en) 2003-07-24

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Legal Events

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WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)