GB2357778B - An apparatus for the hybridization of cells - Google Patents

An apparatus for the hybridization of cells

Info

Publication number
GB2357778B
GB2357778B GB0027557A GB0027557A GB2357778B GB 2357778 B GB2357778 B GB 2357778B GB 0027557 A GB0027557 A GB 0027557A GB 0027557 A GB0027557 A GB 0027557A GB 2357778 B GB2357778 B GB 2357778B
Authority
GB
United Kingdom
Prior art keywords
hybridization
chamber
frit
filter
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0027557A
Other versions
GB2357778A (en
GB0027557D0 (en
Inventor
Lothar Poschen
Peter Klauth
Ralf Wilhelm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Forschungszentrum Juelich GmbH
Original Assignee
Forschungszentrum Juelich GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Juelich GmbH filed Critical Forschungszentrum Juelich GmbH
Publication of GB0027557D0 publication Critical patent/GB0027557D0/en
Publication of GB2357778A publication Critical patent/GB2357778A/en
Application granted granted Critical
Publication of GB2357778B publication Critical patent/GB2357778B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B01L2300/0618—Holders integrated in container to position an object for removable separation walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Mechanical Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

An apparatus for the hybridization of cells comprises a hybridization chamber (1) which is equipped at its base with a frit (4) and with a filter (3) which rests on the frit (4). The filter is fixed, via a sealing ring (8), by a bushing with an external screw thread which can be screwed into a second chamber (2). The second chamber (2) can be heated and the hybridization chamber (1) can be closed so that it is light-tight. Means for removing liquids (6) by suction adjoin the underside of the glass frit (4).
GB0027557A 1999-11-13 2000-11-10 An apparatus for the hybridization of cells Expired - Fee Related GB2357778B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE1999154713 DE19954713B4 (en) 1999-11-13 1999-11-13 Device for hybridization of cells

Publications (3)

Publication Number Publication Date
GB0027557D0 GB0027557D0 (en) 2000-12-27
GB2357778A GB2357778A (en) 2001-07-04
GB2357778B true GB2357778B (en) 2003-09-03

Family

ID=7928978

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0027557A Expired - Fee Related GB2357778B (en) 1999-11-13 2000-11-10 An apparatus for the hybridization of cells

Country Status (3)

Country Link
DE (1) DE19954713B4 (en)
GB (1) GB2357778B (en)
NL (1) NL1016565C2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10223884B4 (en) * 2002-05-29 2004-06-03 Christian Germer Variable multiple filtration and incubation device for processing microbiological and comparable samples

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0122581A2 (en) * 1983-04-15 1984-10-24 TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION Process for isolating bacteria in blood
US5848977A (en) * 1996-02-16 1998-12-15 Inphocyte, Inc. Sample holder for cells
US5874004A (en) * 1996-06-19 1999-02-23 Sheila H. Dewitt Phase separation filter device
WO1999023468A1 (en) * 1997-11-04 1999-05-14 Monogen, Inc. Method and apparatus for mixing and separating particulate matter from a liquid specimen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19725894A1 (en) * 1997-06-19 1998-12-24 Biotechnolog Forschung Gmbh Differential vacuum chamber

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0122581A2 (en) * 1983-04-15 1984-10-24 TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION Process for isolating bacteria in blood
US5848977A (en) * 1996-02-16 1998-12-15 Inphocyte, Inc. Sample holder for cells
US5874004A (en) * 1996-06-19 1999-02-23 Sheila H. Dewitt Phase separation filter device
WO1999023468A1 (en) * 1997-11-04 1999-05-14 Monogen, Inc. Method and apparatus for mixing and separating particulate matter from a liquid specimen

Also Published As

Publication number Publication date
NL1016565C2 (en) 2001-06-05
DE19954713B4 (en) 2006-02-09
DE19954713A1 (en) 2001-05-31
GB2357778A (en) 2001-07-04
NL1016565A1 (en) 2001-05-15
GB0027557D0 (en) 2000-12-27

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20091110