GB2295394A - Vaccine Compositions - Google Patents
Vaccine CompositionsInfo
- Publication number
- GB2295394A GB2295394A GB9601739A GB9601739A GB2295394A GB 2295394 A GB2295394 A GB 2295394A GB 9601739 A GB9601739 A GB 9601739A GB 9601739 A GB9601739 A GB 9601739A GB 2295394 A GB2295394 A GB 2295394A
- Authority
- GB
- United Kingdom
- Prior art keywords
- fusion proteins
- protein
- vaccine compositions
- tetc
- het
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 4
- 108020001507 fusion proteins Proteins 0.000 abstract 4
- 241000894006 Bacteria Species 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108010055044 Tetanus Toxin Proteins 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000002238 attenuated effect Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- KZNQNBZMBZJQJO-YFKPBYRVSA-N glyclproline Chemical compound NCC(=O)N1CCC[C@H]1C(O)=O KZNQNBZMBZJQJO-YFKPBYRVSA-N 0.000 abstract 1
- 108010077515 glycylproline Proteins 0.000 abstract 1
- 229940118376 tetanus toxin Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1088—Glutathione transferase (2.5.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a DNA construct comprising a DNA sequence encoding a fusion protein of the formula: TetC-(Z)a-Het, wherein: TetC is the C fragment of tetanus toxin, or a protein comprising the epitopes thereof; Het is a heterologous protein, Z is an amino acid, and a is zero or a positive integer, provided that (Z)a does not include the sequence Gly-Pro. The invention also provides replicable expression vectors containing the constructs, bacteria transformed with the constructs, the fusion proteins per se and vaccine compositions formed from the fusion proteins or attenuated bacteria expressing the fusion proteins. <IMAGE>
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9601739A GB2295394B (en) | 1994-01-31 | 1994-07-29 | DNA encoding a fusion protein comprising the C fragment of tetanus toxin |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9401787A GB9401787D0 (en) | 1994-01-31 | 1994-01-31 | Vaccine compositions |
GB9601739A GB2295394B (en) | 1994-01-31 | 1994-07-29 | DNA encoding a fusion protein comprising the C fragment of tetanus toxin |
PCT/GB1994/001647 WO1995004151A2 (en) | 1993-07-30 | 1994-07-29 | Vaccine compositions containing recombinant tetc-fusion proteins |
Publications (3)
Publication Number | Publication Date |
---|---|
GB9601739D0 GB9601739D0 (en) | 1996-03-27 |
GB2295394A true GB2295394A (en) | 1996-05-29 |
GB2295394B GB2295394B (en) | 1997-09-24 |
Family
ID=26304238
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB9601739A Revoked GB2295394B (en) | 1994-01-31 | 1994-07-29 | DNA encoding a fusion protein comprising the C fragment of tetanus toxin |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB2295394B (en) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989006974A2 (en) * | 1988-02-01 | 1989-08-10 | Praxis Biologics, Inc. | T-cell epitope as carriers molecule for conjugate vaccines |
EP0432965A1 (en) * | 1989-12-08 | 1991-06-19 | Smithkline Beecham Corporation | Malaria vaccine |
WO1991009621A1 (en) * | 1989-12-29 | 1991-07-11 | Institut Pasteur | PEPTIDE FRAGMENT COMPRISING A SEQUENCE FROM THE 28kDa PROTEIN OF SCHISTOSOMA MANSONI, AND VACCINATING AND/OR THERAPEUTIC COMPOSITIONS COMPRISING SAID FRAGMENT |
WO1992015689A1 (en) * | 1991-03-05 | 1992-09-17 | The Wellcome Foundation Limited | Expression of recombinant proteins in attenuated bacteria |
WO1993008290A1 (en) * | 1991-10-16 | 1993-04-29 | University Of Saskatchewan | Enhanced immunogenicity using leukotoxin chimeras |
WO1994003615A1 (en) * | 1992-07-31 | 1994-02-17 | Medeva Holdings B.V. | Expression of recombinant fusion proteins in attenuated bacteria |
-
1994
- 1994-07-29 GB GB9601739A patent/GB2295394B/en not_active Revoked
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1989006974A2 (en) * | 1988-02-01 | 1989-08-10 | Praxis Biologics, Inc. | T-cell epitope as carriers molecule for conjugate vaccines |
EP0432965A1 (en) * | 1989-12-08 | 1991-06-19 | Smithkline Beecham Corporation | Malaria vaccine |
WO1991009621A1 (en) * | 1989-12-29 | 1991-07-11 | Institut Pasteur | PEPTIDE FRAGMENT COMPRISING A SEQUENCE FROM THE 28kDa PROTEIN OF SCHISTOSOMA MANSONI, AND VACCINATING AND/OR THERAPEUTIC COMPOSITIONS COMPRISING SAID FRAGMENT |
WO1992015689A1 (en) * | 1991-03-05 | 1992-09-17 | The Wellcome Foundation Limited | Expression of recombinant proteins in attenuated bacteria |
WO1993008290A1 (en) * | 1991-10-16 | 1993-04-29 | University Of Saskatchewan | Enhanced immunogenicity using leukotoxin chimeras |
WO1994003615A1 (en) * | 1992-07-31 | 1994-02-17 | Medeva Holdings B.V. | Expression of recombinant fusion proteins in attenuated bacteria |
Non-Patent Citations (4)
Title |
---|
BIO/TECHNOLOGY, Vol. 10, No. 8, 1992, pages 888-892 * |
FEMS Symposium, No. 73, "Bacterial Protein Toxins", published 1994, Freer, J. et al (Ed.) * |
J. IMMUNOLOGY, Vol. 141, No. 5, 1988, pages 1687-1694 * |
NUCLEIC ACIDS RESEARCH, Vol. 19, No. 11, 1991, pages 2889-2892 * |
Also Published As
Publication number | Publication date |
---|---|
GB9601739D0 (en) | 1996-03-27 |
GB2295394B (en) | 1997-09-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
773K | Patent revoked under sect. 73(2)/1977 |
Free format text: PATENT REVOKED ON 20030915 |