GB2235291A - Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics - Google Patents

Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Info

Publication number
GB2235291A
GB2235291A GB8925142A GB8925142A GB2235291A GB 2235291 A GB2235291 A GB 2235291A GB 8925142 A GB8925142 A GB 8925142A GB 8925142 A GB8925142 A GB 8925142A GB 2235291 A GB2235291 A GB 2235291A
Authority
GB
United Kingdom
Prior art keywords
estimation
antibiotics
reduced
analyte
causing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB8925142A
Other versions
GB8925142D0 (en
GB2235291B (en
Inventor
Anthony Atkinson
Julie Miller
Michael Denis Scawen
Robert Stewart Campbell
Christine Helen Morris
Peter Michael Hammond
Christopher Philip Price
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HEALTH LAB SERVICE BOARD
Public Health Laboratory Service Board
Original Assignee
HEALTH LAB SERVICE BOARD
Public Health Laboratory Service Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HEALTH LAB SERVICE BOARD, Public Health Laboratory Service Board filed Critical HEALTH LAB SERVICE BOARD
Publication of GB8925142D0 publication Critical patent/GB8925142D0/en
Publication of GB2235291A publication Critical patent/GB2235291A/en
Application granted granted Critical
Publication of GB2235291B publication Critical patent/GB2235291B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Method for the estimation of an analyte which can be acylated which includes acylating the analyte with an acylcoenzyme A to liberate Co A, causing the Co A to react with 2-oxoglutarate in the presence of NAD and 2-oxoglutarate dehydrogenase to form NADH, and causing the NADH to reduce a further compound, the amount of reduction being measured. Preferably the acylation is an acetylation and the further compound is a tetrazolium salt which is reduced to a formazan. The method is suitable for the estimation of antibiotics which can be acetylated, e.g. thiamphenicol, chloramphenicol and gentamicin. A preferred tetrazolium salt is 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl)-tetrazolium chloride reduced in the presence of 1-methoxy-phenazinium methosulphate.
GB8925142A 1987-05-08 1989-11-03 Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics Expired - Fee Related GB2235291B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878710882A GB8710882D0 (en) 1987-05-08 1987-05-08 Estimation of reduced pyridine nucleotides
PCT/GB1988/000353 WO1988008882A1 (en) 1987-05-08 1988-05-05 Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Publications (3)

Publication Number Publication Date
GB8925142D0 GB8925142D0 (en) 1990-11-14
GB2235291A true GB2235291A (en) 1991-02-27
GB2235291B GB2235291B (en) 1992-01-02

Family

ID=10617004

Family Applications (2)

Application Number Title Priority Date Filing Date
GB878710882A Pending GB8710882D0 (en) 1987-05-08 1987-05-08 Estimation of reduced pyridine nucleotides
GB8925142A Expired - Fee Related GB2235291B (en) 1987-05-08 1989-11-03 Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB878710882A Pending GB8710882D0 (en) 1987-05-08 1987-05-08 Estimation of reduced pyridine nucleotides

Country Status (5)

Country Link
EP (1) EP0357661A1 (en)
JP (1) JPH03500001A (en)
AU (1) AU611684B2 (en)
GB (2) GB8710882D0 (en)
WO (1) WO1988008882A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8908182D0 (en) * 1989-04-12 1989-05-24 Health Lab Service Board Method for assay of albumin
US5126275A (en) * 1990-09-19 1992-06-30 Miles Inc. Analytical method using tetrazolium salt indicators having a reflectance plateau
US6225074B1 (en) * 1997-08-18 2001-05-01 Dennis Wright Direct chloramphenicol acetyl transferase assay

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0007058A1 (en) * 1978-07-18 1980-01-23 Roche Diagnostics GmbH Method and reagent for the determination of glycerol
WO1981001419A1 (en) * 1979-11-13 1981-05-28 Wisconsin Alumni Res Found Method for in activating and monitoring antibiotics and assaying for aminoglycosides
EP0034213A1 (en) * 1980-02-13 1981-08-26 Ivan Endre Modrovich A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay
US4318980A (en) * 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
EP0149853A2 (en) * 1983-12-28 1985-07-31 Wako Pure Chemical Industries, Ltd. Process for measuring activity of dehydrogenase

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3634525A1 (en) * 1986-10-10 1988-04-21 Miles Lab TEST MEANS AND INDICATORS FOR DETECTING THIOL GROUPS AND METHOD FOR THE PRODUCTION THEREOF
GB8728296D0 (en) * 1987-12-03 1988-01-06 Health Lab Service Board Assay of sailicylates/reduced pyridine nucleotides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4318980A (en) * 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
EP0007058A1 (en) * 1978-07-18 1980-01-23 Roche Diagnostics GmbH Method and reagent for the determination of glycerol
WO1981001419A1 (en) * 1979-11-13 1981-05-28 Wisconsin Alumni Res Found Method for in activating and monitoring antibiotics and assaying for aminoglycosides
EP0034213A1 (en) * 1980-02-13 1981-08-26 Ivan Endre Modrovich A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay
EP0149853A2 (en) * 1983-12-28 1985-07-31 Wako Pure Chemical Industries, Ltd. Process for measuring activity of dehydrogenase

Also Published As

Publication number Publication date
AU611684B2 (en) 1991-06-20
GB8925142D0 (en) 1990-11-14
AU1706588A (en) 1988-12-06
WO1988008882A1 (en) 1988-11-17
GB8710882D0 (en) 1987-06-10
EP0357661A1 (en) 1990-03-14
GB2235291B (en) 1992-01-02
JPH03500001A (en) 1991-01-10

Similar Documents

Publication Publication Date Title
KR850000233A (en) 2-enzymatic toothpaste
HUP0101809A3 (en) Inhibitors of type 5 and type 3 17betha-hydroxysteroid dehydrogenase and methods for their use
MY116028A (en) Diagnotics based on tetrazolium compounds
EP0101230A3 (en) Ferrous sulfide dissolution in acid with lowered gas formation
NZ298123A (en) Aqueous composition of hydrogen peroxide or an organic peracid with a ph of 2 or less to remove and/or bleach limescale at a surface
EP0837949A4 (en) Electrochemiluminescent enzyme biosensors
RU92016568A (en) METHOD OF OBTAINING BETA LACTAM DERIVATIVES
GB2204698B (en) Amperometric method for the quantitative determination of 1,4- dihydronicotinamide adenine dinucleotide (nadh) in solution
DE3666226D1 (en) Process for the preparation of 1,2-dithiolan-3-pentanoic acid (thioctic acid)
EP0347864A3 (en) Anti-atherogenic agents
GB2235291A (en) Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics
IL111184A (en) Crystalline inclusion complex of diclofenac with unsubstituted beta-cyclodextrin
AU5291299A (en) Process for preparing n,n,6- trimethyl-2 -(4-methylphenyl)- imidazo-(1,2-a) -pyridine-3- acetamide and salts thereof
AU6452890A (en) Trigylcerides containing saturated fatty acids having 20 to 24 carbon atoms useful in lowering blood cholesterol levels
DK165981C (en) ISOXAZOLD DERIVATIVES, PROCEDURES FOR THEIR PREPARATION AND PHARMACEUTICALS CONTAINING THESE COMPOUNDS
CA2144206A1 (en) Ice Melter with Coating of Alpha-Methyl Glucoside Mixture and Method of Making Same
MX9704309A (en) Cosmetic stick.
EP1106713A3 (en) Method for determining the pickling time of a scaled metal strip
JPS6422865A (en) Fluorine-containing rennin inhibitor
Chang et al. Involvement of lysine residue in the nucleotide binding of pigeon liver malic enzyme: modification with affinity label periodate-oxidized NADP
AU563426B2 (en) 3-alkoxy-2(n-pyrrolidino)-n-pyridyl-n-furyl (or thienyl) methyl propylamines
EP0138530A3 (en) Method for the estimation of salicylates or reduced pyridine nucleotides
ZA846478B (en) Process and reagent for determining transaminasae
EP0351774A3 (en) Determination of blood ammonia levels
GB2104073B (en) Fluorinated diamino-heptene and -heptyne derivatives

Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19930505