GB2235291A - Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics - Google Patents
Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibioticsInfo
- Publication number
- GB2235291A GB2235291A GB8925142A GB8925142A GB2235291A GB 2235291 A GB2235291 A GB 2235291A GB 8925142 A GB8925142 A GB 8925142A GB 8925142 A GB8925142 A GB 8925142A GB 2235291 A GB2235291 A GB 2235291A
- Authority
- GB
- United Kingdom
- Prior art keywords
- estimation
- antibiotics
- reduced
- analyte
- causing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Method for the estimation of an analyte which can be acylated which includes acylating the analyte with an acylcoenzyme A to liberate Co A, causing the Co A to react with 2-oxoglutarate in the presence of NAD and 2-oxoglutarate dehydrogenase to form NADH, and causing the NADH to reduce a further compound, the amount of reduction being measured. Preferably the acylation is an acetylation and the further compound is a tetrazolium salt which is reduced to a formazan. The method is suitable for the estimation of antibiotics which can be acetylated, e.g. thiamphenicol, chloramphenicol and gentamicin. A preferred tetrazolium salt is 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl)-tetrazolium chloride reduced in the presence of 1-methoxy-phenazinium methosulphate.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB878710882A GB8710882D0 (en) | 1987-05-08 | 1987-05-08 | Estimation of reduced pyridine nucleotides |
PCT/GB1988/000353 WO1988008882A1 (en) | 1987-05-08 | 1988-05-05 | Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics |
Publications (3)
Publication Number | Publication Date |
---|---|
GB8925142D0 GB8925142D0 (en) | 1990-11-14 |
GB2235291A true GB2235291A (en) | 1991-02-27 |
GB2235291B GB2235291B (en) | 1992-01-02 |
Family
ID=10617004
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB878710882A Pending GB8710882D0 (en) | 1987-05-08 | 1987-05-08 | Estimation of reduced pyridine nucleotides |
GB8925142A Expired - Fee Related GB2235291B (en) | 1987-05-08 | 1989-11-03 | Method for the estimation of reduced pyridine nucleotides and use of the method for estimation of co-a and antibiotics |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB878710882A Pending GB8710882D0 (en) | 1987-05-08 | 1987-05-08 | Estimation of reduced pyridine nucleotides |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0357661A1 (en) |
JP (1) | JPH03500001A (en) |
AU (1) | AU611684B2 (en) |
GB (2) | GB8710882D0 (en) |
WO (1) | WO1988008882A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8908182D0 (en) * | 1989-04-12 | 1989-05-24 | Health Lab Service Board | Method for assay of albumin |
US5126275A (en) * | 1990-09-19 | 1992-06-30 | Miles Inc. | Analytical method using tetrazolium salt indicators having a reflectance plateau |
US6225074B1 (en) * | 1997-08-18 | 2001-05-01 | Dennis Wright | Direct chloramphenicol acetyl transferase assay |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0007058A1 (en) * | 1978-07-18 | 1980-01-23 | Roche Diagnostics GmbH | Method and reagent for the determination of glycerol |
WO1981001419A1 (en) * | 1979-11-13 | 1981-05-28 | Wisconsin Alumni Res Found | Method for in activating and monitoring antibiotics and assaying for aminoglycosides |
EP0034213A1 (en) * | 1980-02-13 | 1981-08-26 | Ivan Endre Modrovich | A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay |
US4318980A (en) * | 1978-04-10 | 1982-03-09 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a cycling reactant as label |
EP0149853A2 (en) * | 1983-12-28 | 1985-07-31 | Wako Pure Chemical Industries, Ltd. | Process for measuring activity of dehydrogenase |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3634525A1 (en) * | 1986-10-10 | 1988-04-21 | Miles Lab | TEST MEANS AND INDICATORS FOR DETECTING THIOL GROUPS AND METHOD FOR THE PRODUCTION THEREOF |
GB8728296D0 (en) * | 1987-12-03 | 1988-01-06 | Health Lab Service Board | Assay of sailicylates/reduced pyridine nucleotides |
-
1987
- 1987-05-08 GB GB878710882A patent/GB8710882D0/en active Pending
-
1988
- 1988-05-05 WO PCT/GB1988/000353 patent/WO1988008882A1/en not_active Application Discontinuation
- 1988-05-05 JP JP50384788A patent/JPH03500001A/en active Pending
- 1988-05-05 EP EP19880903899 patent/EP0357661A1/en not_active Withdrawn
- 1988-05-05 AU AU17065/88A patent/AU611684B2/en not_active Ceased
-
1989
- 1989-11-03 GB GB8925142A patent/GB2235291B/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4318980A (en) * | 1978-04-10 | 1982-03-09 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a cycling reactant as label |
EP0007058A1 (en) * | 1978-07-18 | 1980-01-23 | Roche Diagnostics GmbH | Method and reagent for the determination of glycerol |
WO1981001419A1 (en) * | 1979-11-13 | 1981-05-28 | Wisconsin Alumni Res Found | Method for in activating and monitoring antibiotics and assaying for aminoglycosides |
EP0034213A1 (en) * | 1980-02-13 | 1981-08-26 | Ivan Endre Modrovich | A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay |
EP0149853A2 (en) * | 1983-12-28 | 1985-07-31 | Wako Pure Chemical Industries, Ltd. | Process for measuring activity of dehydrogenase |
Also Published As
Publication number | Publication date |
---|---|
AU611684B2 (en) | 1991-06-20 |
GB8925142D0 (en) | 1990-11-14 |
AU1706588A (en) | 1988-12-06 |
WO1988008882A1 (en) | 1988-11-17 |
GB8710882D0 (en) | 1987-06-10 |
EP0357661A1 (en) | 1990-03-14 |
GB2235291B (en) | 1992-01-02 |
JPH03500001A (en) | 1991-01-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930505 |