GB2220003B - A method and device for separating motile cells from less motile cells in a mixed cell sample. - Google Patents

A method and device for separating motile cells from less motile cells in a mixed cell sample.

Info

Publication number
GB2220003B
GB2220003B GB8815376A GB8815376A GB2220003B GB 2220003 B GB2220003 B GB 2220003B GB 8815376 A GB8815376 A GB 8815376A GB 8815376 A GB8815376 A GB 8815376A GB 2220003 B GB2220003 B GB 2220003B
Authority
GB
United Kingdom
Prior art keywords
motile cells
cell sample
mixed cell
separating
less
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB8815376A
Other versions
GB2220003A (en
GB8815376D0 (en
Inventor
Fu-Nan Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
WANG FU NAN
Original Assignee
WANG FU NAN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by WANG FU NAN filed Critical WANG FU NAN
Publication of GB8815376D0 publication Critical patent/GB8815376D0/en
Priority claimed from AU24074/88A external-priority patent/AU2407488A/en
Priority to AU24074/88A priority Critical patent/AU2407488A/en
Priority to FR8814107A priority patent/FR2638323B1/en
Priority to DE3904889A priority patent/DE3904889A1/en
Priority to CA000599317A priority patent/CA1329786C/en
Publication of GB2220003A publication Critical patent/GB2220003A/en
Priority to AU22806/92A priority patent/AU651490B2/en
Publication of GB2220003B publication Critical patent/GB2220003B/en
Application granted granted Critical
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/52Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56Labware specially adapted for transferring fluids
    • B01L3/561Tubes; Conduits
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0832Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838Capillaries
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406Moving fluids with specific forces or mechanical means specific forces capillary forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
GB8815376A 1988-06-28 1988-06-28 A method and device for separating motile cells from less motile cells in a mixed cell sample. Expired - Fee Related GB2220003B (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU24074/88A AU2407488A (en) 1988-06-28 1988-10-20 Sperm/germ and germ-free y/x sperm separating systems
FR8814107A FR2638323B1 (en) 1988-06-28 1988-10-28 PROCESS AND DEVICE FOR TREATING SPERM
DE3904889A DE3904889A1 (en) 1988-06-28 1989-02-17 DEVICE AND METHOD FOR SEPARATING MOVABLE CELLS FROM LESS MOVABLE OR IMMOVABLE CELLS IN A SAMPLE CONTAINING A CELL MIXTURE
CA000599317A CA1329786C (en) 1988-06-28 1989-05-10 Method and/or device for separating motile cells from non-motile, or less motile cells in a mixed cell sample
AU22806/92A AU651490B2 (en) 1988-06-28 1992-09-07 Method and device for separating motile cells from non-motile, or less motile cells in a mixed cell sample

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU24074/88A AU2407488A (en) 1988-06-28 1988-10-20 Sperm/germ and germ-free y/x sperm separating systems
CA000599317A CA1329786C (en) 1988-06-28 1989-05-10 Method and/or device for separating motile cells from non-motile, or less motile cells in a mixed cell sample
AU22806/92A AU651490B2 (en) 1988-06-28 1992-09-07 Method and device for separating motile cells from non-motile, or less motile cells in a mixed cell sample

Publications (3)

Publication Number Publication Date
GB8815376D0 GB8815376D0 (en) 1988-08-03
GB2220003A GB2220003A (en) 1989-12-28
GB2220003B true GB2220003B (en) 1993-03-10

Family

ID=27152763

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8815376A Expired - Fee Related GB2220003B (en) 1988-06-28 1988-06-28 A method and device for separating motile cells from less motile cells in a mixed cell sample.

Country Status (1)

Country Link
GB (1) GB2220003B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
DE69319427T2 (en) * 1992-05-01 1998-12-10 Univ Pennsylvania ANALYSIS OF MEASURING THE FLOW RESISTANCE
US5296375A (en) * 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
EP0683645B1 (en) * 1993-02-09 1997-06-11 ZECH, Josef Device for removing sperm cells from seminal fluid
GB9817795D0 (en) * 1998-08-14 1998-10-14 Genosis Inc A method and device for separation and detection of spermatozoa in a sample
AT410280B (en) * 2001-10-05 2003-03-25 Zech Josef Dr Apparatus for isolating spermatozoa from ejaculate has annular connector with parallel channels, down which spermatozoa swim from annular trough of ejaculate into central container filled with nutrient

Also Published As

Publication number Publication date
GB2220003A (en) 1989-12-28
GB8815376D0 (en) 1988-08-03

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19990628