GB2179448B - Improved sandwich hybridisation technique for the detection of nucleotide sequences - Google Patents

Improved sandwich hybridisation technique for the detection of nucleotide sequences

Info

Publication number
GB2179448B
GB2179448B GB8617718A GB8617718A GB2179448B GB 2179448 B GB2179448 B GB 2179448B GB 8617718 A GB8617718 A GB 8617718A GB 8617718 A GB8617718 A GB 8617718A GB 2179448 B GB2179448 B GB 2179448B
Authority
GB
United Kingdom
Prior art keywords
detection
nucleotide sequences
hybridisation technique
improved sandwich
sandwich hybridisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB8617718A
Other versions
GB2179448A (en
GB8617718D0 (en
Inventor
Alan David Blair Malcolm
Jane Alison Langdale
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blair Malcolm A D
Original Assignee
Blair Malcolm A D
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB848432118A external-priority patent/GB8432118D0/en
Application filed by Blair Malcolm A D filed Critical Blair Malcolm A D
Priority to GB8617718A priority Critical patent/GB2179448B/en
Publication of GB8617718D0 publication Critical patent/GB8617718D0/en
Publication of GB2179448A publication Critical patent/GB2179448A/en
Application granted granted Critical
Publication of GB2179448B publication Critical patent/GB2179448B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB8617718A 1984-12-19 1985-12-19 Improved sandwich hybridisation technique for the detection of nucleotide sequences Expired GB2179448B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8617718A GB2179448B (en) 1984-12-19 1985-12-19 Improved sandwich hybridisation technique for the detection of nucleotide sequences

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB848432118A GB8432118D0 (en) 1984-12-19 1984-12-19 Sandwich hybridisation technique
PCT/GB1985/000591 WO1986003782A1 (en) 1984-12-19 1985-12-19 Improved sandwich hybridisation technique for the detection of nucleotide sequences
GB8617718A GB2179448B (en) 1984-12-19 1985-12-19 Improved sandwich hybridisation technique for the detection of nucleotide sequences

Publications (3)

Publication Number Publication Date
GB8617718D0 GB8617718D0 (en) 1986-08-28
GB2179448A GB2179448A (en) 1987-03-04
GB2179448B true GB2179448B (en) 1989-06-07

Family

ID=26288596

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8617718A Expired GB2179448B (en) 1984-12-19 1985-12-19 Improved sandwich hybridisation technique for the detection of nucleotide sequences

Country Status (1)

Country Link
GB (1) GB2179448B (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0079139A1 (en) * 1981-10-16 1983-05-18 Orion-yhtymä Oy A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor
US4395486A (en) * 1981-08-19 1983-07-26 Medical College Of Ga. Research Inst., Inc. Method for the direct analysis of sickle cell anemia
EP0124221A1 (en) * 1983-03-09 1984-11-07 Alan David Blair Malcolm Method of detecting a polynucleotide sequence and labelled polynucleotides useful therein
WO1984004332A1 (en) * 1983-04-29 1984-11-08 Nat Res Dev Method of determining nucleotide sequences in cells and of isolating nucleic acids from cells
EP0130515A2 (en) * 1983-07-05 1985-01-09 Molecular Diagnostics, Inc. Testing DNA samples for particular nucleotide sequences
EP0130523A2 (en) * 1983-07-05 1985-01-09 Molecular Diagnostics, Inc. Immobilized nucleic acid probe and solid support for nucleic acids
EP0070687B1 (en) * 1981-07-17 1985-10-16 Amoco Corporation Light-emitting polynucleotide hybridization diagnostic method

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0070687B1 (en) * 1981-07-17 1985-10-16 Amoco Corporation Light-emitting polynucleotide hybridization diagnostic method
US4395486A (en) * 1981-08-19 1983-07-26 Medical College Of Ga. Research Inst., Inc. Method for the direct analysis of sickle cell anemia
EP0079139A1 (en) * 1981-10-16 1983-05-18 Orion-yhtymä Oy A method and reagent combination for the identification of microorganisms and the use of sandwich hybridization of nucleic acids therefor
EP0124221A1 (en) * 1983-03-09 1984-11-07 Alan David Blair Malcolm Method of detecting a polynucleotide sequence and labelled polynucleotides useful therein
WO1984004332A1 (en) * 1983-04-29 1984-11-08 Nat Res Dev Method of determining nucleotide sequences in cells and of isolating nucleic acids from cells
EP0130515A2 (en) * 1983-07-05 1985-01-09 Molecular Diagnostics, Inc. Testing DNA samples for particular nucleotide sequences
EP0130523A2 (en) * 1983-07-05 1985-01-09 Molecular Diagnostics, Inc. Immobilized nucleic acid probe and solid support for nucleic acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Chemical Abstracts, volume 96, no. 23, 7 June 1982 Abstract no. 196141w. *
Proceedings of the National Academy of Science USA volume 80January 1983 pages 278-2 *

Also Published As

Publication number Publication date
GB2179448A (en) 1987-03-04
GB8617718D0 (en) 1986-08-28

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee