GB2121410A - 9 alpha -oxo and hydroxy carbacyclins - Google Patents
9 alpha -oxo and hydroxy carbacyclins Download PDFInfo
- Publication number
- GB2121410A GB2121410A GB08314615A GB8314615A GB2121410A GB 2121410 A GB2121410 A GB 2121410A GB 08314615 A GB08314615 A GB 08314615A GB 8314615 A GB8314615 A GB 8314615A GB 2121410 A GB2121410 A GB 2121410A
- Authority
- GB
- United Kingdom
- Prior art keywords
- mixture
- group
- general formula
- oxo
- enyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000002887 hydroxy group Chemical group [H]O* 0.000 title description 2
- -1 carbonyl hydroxymethylene Chemical group 0.000 claims abstract description 117
- 150000001875 compounds Chemical class 0.000 claims abstract description 115
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract description 58
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims abstract description 43
- 125000000217 alkyl group Chemical group 0.000 claims abstract description 36
- 150000003180 prostaglandins Chemical class 0.000 claims abstract description 33
- 238000002360 preparation method Methods 0.000 claims abstract description 31
- 150000003839 salts Chemical class 0.000 claims abstract description 30
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 19
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims abstract description 19
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims abstract description 18
- 125000000753 cycloalkyl group Chemical group 0.000 claims abstract description 17
- 231100000252 nontoxic Toxicity 0.000 claims abstract description 15
- 230000003000 nontoxic effect Effects 0.000 claims abstract description 15
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims abstract description 13
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims abstract description 8
- 125000002947 alkylene group Chemical group 0.000 claims abstract description 7
- 229940006138 antiglaucoma drug and miotics prostaglandin analogues Drugs 0.000 claims abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 6
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000005977 Ethylene Substances 0.000 claims abstract description 5
- 239000001301 oxygen Substances 0.000 claims abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims abstract description 4
- 125000003545 alkoxy group Chemical group 0.000 claims abstract description 3
- 125000005843 halogen group Chemical group 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims description 260
- 238000000034 method Methods 0.000 claims description 42
- 239000002253 acid Substances 0.000 claims description 31
- 230000008569 process Effects 0.000 claims description 27
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 125000005678 ethenylene group Chemical group [H]C([*:1])=C([H])[*:2] 0.000 claims description 10
- 125000000816 ethylene group Chemical group [H]C([H])([*:1])C([H])([H])[*:2] 0.000 claims description 10
- 229910052783 alkali metal Inorganic materials 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 8
- 230000003647 oxidation Effects 0.000 claims description 7
- 238000007254 oxidation reaction Methods 0.000 claims description 7
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 claims description 5
- 238000005903 acid hydrolysis reaction Methods 0.000 claims description 5
- 150000001340 alkali metals Chemical group 0.000 claims description 5
- 238000011065 in-situ storage Methods 0.000 claims description 5
- 239000008194 pharmaceutical composition Substances 0.000 claims description 5
- 239000004480 active ingredient Substances 0.000 claims description 4
- JPJALAQPGMAKDF-UHFFFAOYSA-N selenium dioxide Chemical compound O=[Se]=O JPJALAQPGMAKDF-UHFFFAOYSA-N 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 claims description 3
- 125000002485 formyl group Chemical group [H]C(*)=O 0.000 claims description 3
- 229910052744 lithium Inorganic materials 0.000 claims description 3
- 125000006239 protecting group Chemical group 0.000 claims description 3
- 125000002252 acyl group Chemical group 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- XEYBRNLFEZDVAW-ARSRFYASSA-N dinoprostone Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)CC(=O)[C@@H]1C\C=C/CCCC(O)=O XEYBRNLFEZDVAW-ARSRFYASSA-N 0.000 claims description 2
- 229960002986 dinoprostone Drugs 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims description 2
- 125000001570 methylene group Chemical group [H]C([H])([*:1])[*:2] 0.000 claims description 2
- 125000004430 oxygen atom Chemical group O* 0.000 claims description 2
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 claims description 2
- 239000008016 pharmaceutical coating Substances 0.000 claims description 2
- XEYBRNLFEZDVAW-UHFFFAOYSA-N prostaglandin E2 Natural products CCCCCC(O)C=CC1C(O)CC(=O)C1CC=CCCCC(O)=O XEYBRNLFEZDVAW-UHFFFAOYSA-N 0.000 claims description 2
- 125000004436 sodium atom Chemical group 0.000 claims description 2
- FGEBEFIJERRKAQ-ZWKOTPCHSA-N 5-hydroxy-7-[(1R,2S)-2-oct-1-enylcyclopentyl]hept-5-enoic acid Chemical compound OC(CCCC(=O)O)=CC[C@H]1CCC[C@@H]1C=CCCCCCC FGEBEFIJERRKAQ-ZWKOTPCHSA-N 0.000 claims 2
- 125000005907 alkyl ester group Chemical group 0.000 claims 1
- 150000002641 lithium Chemical group 0.000 claims 1
- LMBFAGIMSUYTBN-MPZNNTNKSA-N teixobactin Chemical compound C([C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H](CCC(N)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)N[C@H]1C(N[C@@H](C)C(=O)N[C@@H](C[C@@H]2NC(=N)NC2)C(=O)N[C@H](C(=O)O[C@H]1C)[C@@H](C)CC)=O)NC)C1=CC=CC=C1 LMBFAGIMSUYTBN-MPZNNTNKSA-N 0.000 claims 1
- 150000003475 thallium Chemical class 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 claims 1
- 101100297345 Caenorhabditis elegans pgl-2 gene Proteins 0.000 abstract description 12
- 239000000543 intermediate Substances 0.000 abstract description 6
- 239000001257 hydrogen Substances 0.000 abstract description 5
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract description 4
- 229940094443 oxytocics prostaglandins Drugs 0.000 abstract description 4
- 230000000144 pharmacologic effect Effects 0.000 abstract description 4
- 206010002383 Angina Pectoris Diseases 0.000 abstract description 3
- 206010003210 Arteriosclerosis Diseases 0.000 abstract description 3
- 206010020772 Hypertension Diseases 0.000 abstract description 3
- 208000007536 Thrombosis Diseases 0.000 abstract description 3
- 208000011775 arteriosclerosis disease Diseases 0.000 abstract description 3
- ABLZXFCXXLZCGV-UHFFFAOYSA-N Phosphorous acid Chemical class OP(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052736 halogen Inorganic materials 0.000 abstract description 2
- 208000010125 myocardial infarction Diseases 0.000 abstract description 2
- 239000005864 Sulphur Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 171
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 168
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 162
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 95
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 93
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 84
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 78
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 72
- NZTVVUIIJPWANB-UHFFFAOYSA-N 3,3a,4,5,6,6a-hexahydro-2h-pentalen-1-one Chemical compound C1CCC2C(=O)CCC21 NZTVVUIIJPWANB-UHFFFAOYSA-N 0.000 description 71
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 58
- 229920006395 saturated elastomer Polymers 0.000 description 52
- ZPVFWPFBNIEHGJ-UHFFFAOYSA-N 2-octanone Chemical compound CCCCCCC(C)=O ZPVFWPFBNIEHGJ-UHFFFAOYSA-N 0.000 description 49
- 239000011780 sodium chloride Substances 0.000 description 47
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 42
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 42
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 40
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 39
- 229910052938 sodium sulfate Inorganic materials 0.000 description 39
- 235000011152 sodium sulphate Nutrition 0.000 description 39
- 239000000741 silica gel Substances 0.000 description 37
- 229910002027 silica gel Inorganic materials 0.000 description 37
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 36
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 33
- 229960000583 acetic acid Drugs 0.000 description 32
- 239000000284 extract Substances 0.000 description 31
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 24
- 239000012362 glacial acetic acid Substances 0.000 description 24
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 22
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 21
- 238000005055 short column chromatography Methods 0.000 description 20
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 18
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 18
- 235000017557 sodium bicarbonate Nutrition 0.000 description 18
- HEDRZPFGACZZDS-MICDWDOJSA-N Trichloro(2H)methane Chemical compound [2H]C(Cl)(Cl)Cl HEDRZPFGACZZDS-MICDWDOJSA-N 0.000 description 16
- 239000012298 atmosphere Substances 0.000 description 16
- 239000007858 starting material Substances 0.000 description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 15
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 15
- 239000003921 oil Substances 0.000 description 14
- 235000019198 oils Nutrition 0.000 description 14
- LEHBURLTIWGHEM-UHFFFAOYSA-N pyridinium chlorochromate Chemical compound [O-][Cr](Cl)(=O)=O.C1=CC=[NH+]C=C1 LEHBURLTIWGHEM-UHFFFAOYSA-N 0.000 description 14
- 239000003960 organic solvent Substances 0.000 description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 12
- WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 12
- 229910052786 argon Inorganic materials 0.000 description 12
- 238000003756 stirring Methods 0.000 description 11
- RHLVCLIPMVJYKS-UHFFFAOYSA-N 3-octanone Chemical compound CCCCCC(=O)CC RHLVCLIPMVJYKS-UHFFFAOYSA-N 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 10
- 239000012300 argon atmosphere Substances 0.000 description 10
- 238000004587 chromatography analysis Methods 0.000 description 10
- 238000001816 cooling Methods 0.000 description 10
- 239000000725 suspension Substances 0.000 description 10
- AEBWATHAIVJLTA-UHFFFAOYSA-N 1,2,3,3a,4,5,6,6a-octahydropentalene Chemical group C1CCC2CCCC21 AEBWATHAIVJLTA-UHFFFAOYSA-N 0.000 description 9
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 9
- 239000010410 layer Substances 0.000 description 9
- 239000011541 reaction mixture Substances 0.000 description 9
- JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- TVMXDCGIABBOFY-UHFFFAOYSA-N octane Chemical compound CCCCCCCC TVMXDCGIABBOFY-UHFFFAOYSA-N 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 230000009467 reduction Effects 0.000 description 8
- 125000003003 spiro group Chemical group 0.000 description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 7
- 235000019341 magnesium sulphate Nutrition 0.000 description 7
- 238000010992 reflux Methods 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- AVZFPOGLYLUAIQ-FOWTUZBSSA-N 4-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-3-cyclohexylprop-1-enyl]-3,3a,4,5,6,6a-hexahydro-2h-pentalen-1-one Chemical compound C1CC(C(CC2)=O)C2C1/C=C/C(O[Si](C)(C)C(C)(C)C)C1CCCCC1 AVZFPOGLYLUAIQ-FOWTUZBSSA-N 0.000 description 6
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 150000007513 acids Chemical class 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- ZCSHNCUQKCANBX-UHFFFAOYSA-N lithium diisopropylamide Chemical compound [Li+].CC(C)[N-]C(C)C ZCSHNCUQKCANBX-UHFFFAOYSA-N 0.000 description 6
- QARBMVPHQWIHKH-UHFFFAOYSA-N methanesulfonyl chloride Chemical compound CS(Cl)(=O)=O QARBMVPHQWIHKH-UHFFFAOYSA-N 0.000 description 6
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 6
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 5
- 230000006978 adaptation Effects 0.000 description 5
- 239000002585 base Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- YBTZROCKNUIONO-UHFFFAOYSA-N methyl 5-oxopentanoate Chemical compound COC(=O)CCCC=O YBTZROCKNUIONO-UHFFFAOYSA-N 0.000 description 5
- 229910000029 sodium carbonate Inorganic materials 0.000 description 5
- 239000012312 sodium hydride Substances 0.000 description 5
- 229910000104 sodium hydride Inorganic materials 0.000 description 5
- 239000008247 solid mixture Substances 0.000 description 5
- LQZCYXCHWNQBKX-UHFFFAOYSA-N 1-dimethoxyphosphorylheptan-2-one Chemical compound CCCCCC(=O)CP(=O)(OC)OC LQZCYXCHWNQBKX-UHFFFAOYSA-N 0.000 description 4
- LSMKBKNQAHPBMR-ACCUITESSA-N 4-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-4-phenoxybut-1-enyl]-3,3a,4,5,6,6a-hexahydro-2h-pentalen-1-one Chemical compound C1CC(C(CC2)=O)C2C1/C=C/C(O[Si](C)(C)C(C)(C)C)COC1=CC=CC=C1 LSMKBKNQAHPBMR-ACCUITESSA-N 0.000 description 4
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- MZRVEZGGRBJDDB-UHFFFAOYSA-N N-Butyllithium Chemical compound [Li]CCCC MZRVEZGGRBJDDB-UHFFFAOYSA-N 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- 241000282898 Sus scrofa Species 0.000 description 4
- 150000001412 amines Chemical class 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000005984 hydrogenation reaction Methods 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000003701 inert diluent Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 4
- 229910052753 mercury Inorganic materials 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- CBENYEJILPJOOQ-CFXALYHXSA-N methyl (5e)-5-[6-[(e)-3-cyclohexyl-3-hydroxyprop-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]pentanoate Chemical compound C1CC2C(=O)C(=C/CCCC(=O)OC)/CC2C1\C=C\C(O)C1CCCCC1 CBENYEJILPJOOQ-CFXALYHXSA-N 0.000 description 4
- GBDDLCNCLVMOPG-BMRADRMJSA-N methyl 5-[6-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-4-phenoxybut-1-enyl]-3-oxo-2,3a,4,5,6,6a-hexahydro-1h-pentalen-2-yl]-5-hydroxypentanoate Chemical compound C1CC2C(=O)C(C(O)CCCC(=O)OC)CC2C1\C=C\C(O[Si](C)(C)C(C)(C)C)COC1=CC=CC=C1 GBDDLCNCLVMOPG-BMRADRMJSA-N 0.000 description 4
- 239000012044 organic layer Substances 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- 238000012746 preparative thin layer chromatography Methods 0.000 description 4
- KAQKFAOMNZTLHT-OZUDYXHBSA-N prostaglandin I2 Chemical class O1\C(=C/CCCC(O)=O)C[C@@H]2[C@@H](/C=C/[C@@H](O)CCCCC)[C@H](O)C[C@@H]21 KAQKFAOMNZTLHT-OZUDYXHBSA-N 0.000 description 4
- 239000012047 saturated solution Substances 0.000 description 4
- 159000000000 sodium salts Chemical class 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- BCNZYOJHNLTNEZ-UHFFFAOYSA-N tert-butyldimethylsilyl chloride Chemical compound CC(C)(C)[Si](C)(C)Cl BCNZYOJHNLTNEZ-UHFFFAOYSA-N 0.000 description 4
- KLBIUKJOZFWCLW-UHFFFAOYSA-N thallium(iii) nitrate Chemical compound [Tl+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O KLBIUKJOZFWCLW-UHFFFAOYSA-N 0.000 description 4
- GQHTUMJGOHRCHB-UHFFFAOYSA-N 2,3,4,6,7,8,9,10-octahydropyrimido[1,2-a]azepine Chemical compound C1CCCCN2CCCN=C21 GQHTUMJGOHRCHB-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 230000001476 alcoholic effect Effects 0.000 description 3
- 210000001772 blood platelet Anatomy 0.000 description 3
- 239000011203 carbon fibre reinforced carbon Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- GNOIPBMMFNIUFM-UHFFFAOYSA-N hexamethylphosphoric triamide Chemical compound CN(C)P(=O)(N(C)C)N(C)C GNOIPBMMFNIUFM-UHFFFAOYSA-N 0.000 description 3
- YNESATAKKCNGOF-UHFFFAOYSA-N lithium bis(trimethylsilyl)amide Chemical compound [Li+].C[Si](C)(C)[N-][Si](C)(C)C YNESATAKKCNGOF-UHFFFAOYSA-N 0.000 description 3
- OQZPXCPZYUHHNZ-KMBKYLCOSA-N methyl (5e)-5-[6-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-4-phenoxybut-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]pentanoate Chemical compound C1CC2C(=O)C(=C/CCCC(=O)OC)/CC2C1\C=C\C(O[Si](C)(C)C(C)(C)C)COC1=CC=CC=C1 OQZPXCPZYUHHNZ-KMBKYLCOSA-N 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 125000003884 phenylalkyl group Chemical group 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910000027 potassium carbonate Inorganic materials 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- SGUKUZOVHSFKPH-YNNPMVKQSA-N prostaglandin G2 Chemical compound C1[C@@H]2OO[C@H]1[C@H](/C=C/[C@@H](OO)CCCCC)[C@H]2C\C=C/CCCC(O)=O SGUKUZOVHSFKPH-YNNPMVKQSA-N 0.000 description 3
- 238000007086 side reaction Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- JABYJIQOLGWMQW-UHFFFAOYSA-N undec-4-ene Chemical compound CCCCCCC=CCCC JABYJIQOLGWMQW-UHFFFAOYSA-N 0.000 description 3
- 239000000080 wetting agent Substances 0.000 description 3
- DSZGRQPWBMFUAR-UHFFFAOYSA-N (4-formyl-1,2,3,3a,4,5,6,6a-octahydropentalen-1-yl) acetate Chemical compound O=CC1CCC2C(OC(=O)C)CCC21 DSZGRQPWBMFUAR-UHFFFAOYSA-N 0.000 description 2
- CZKVJWJIZCCNKK-UHFFFAOYSA-N (5-methoxy-2,3,3a,4,7,7a-hexahydro-1h-inden-1-yl) acetate Chemical compound C1C(OC)=CCC2C(OC(C)=O)CCC21 CZKVJWJIZCCNKK-UHFFFAOYSA-N 0.000 description 2
- FTZNMXSYERMMBC-UHFFFAOYSA-N 1-cyclohexyl-2-dimethoxyphosphorylethanone Chemical compound COP(=O)(OC)CC(=O)C1CCCCC1 FTZNMXSYERMMBC-UHFFFAOYSA-N 0.000 description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- YJKWLBQFRJUZOR-JXMROGBWSA-N 4-[(e)-3-cyclohexyl-3-hydroxyprop-1-enyl]-3,3a,4,5,6,6a-hexahydro-2h-pentalen-1-one Chemical compound C1CC(C(CC2)=O)C2C1/C=C/C(O)C1CCCCC1 YJKWLBQFRJUZOR-JXMROGBWSA-N 0.000 description 2
- BXRLCILKEXDBMP-SOFGYWHQSA-N 4-[(e)-3-hydroxy-4-phenoxybut-1-enyl]-3,3a,4,5,6,6a-hexahydro-2h-pentalen-1-one Chemical compound C1CC(C(CC2)=O)C2C1/C=C/C(O)COC1=CC=CC=C1 BXRLCILKEXDBMP-SOFGYWHQSA-N 0.000 description 2
- PCPHGSPHIDFZGD-UHFFFAOYSA-N 4-acetyloxy-1,2,3,3a,4,5,6,6a-octahydropentalene-1-carboxylic acid Chemical compound C1CC(C(O)=O)C2C1C(OC(=O)C)CC2 PCPHGSPHIDFZGD-UHFFFAOYSA-N 0.000 description 2
- ASEKSZLFLXQGBG-UHFFFAOYSA-N 5-methoxy-2,3,3a,4,7,7a-hexahydroinden-1-one Chemical compound C1C(OC)=CCC2C(=O)CCC21 ASEKSZLFLXQGBG-UHFFFAOYSA-N 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 2
- BZKFMUIJRXWWQK-UHFFFAOYSA-N Cyclopentenone Chemical compound O=C1CCC=C1 BZKFMUIJRXWWQK-UHFFFAOYSA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 2
- IYTXKIXETAELAV-UHFFFAOYSA-N Nonan-3-one Chemical compound CCCCCCC(=O)CC IYTXKIXETAELAV-UHFFFAOYSA-N 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 2
- NFFWAUONZFDLNM-UHFFFAOYSA-N [4-(hydroxymethyl)-1,2,3,3a,4,5,6,6a-octahydropentalen-1-yl] acetate Chemical compound OCC1CCC2C(OC(=O)C)CCC21 NFFWAUONZFDLNM-UHFFFAOYSA-N 0.000 description 2
- DDVPUYBOTNWVSP-VQHVLOKHSA-N [4-[(e)-3-oxooct-1-enyl]-1,2,3,3a,4,5,6,6a-octahydropentalen-1-yl] acetate Chemical compound CC(=O)OC1CCC2C(/C=C/C(=O)CCCCC)CCC21 DDVPUYBOTNWVSP-VQHVLOKHSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 229910000102 alkali metal hydride Inorganic materials 0.000 description 2
- 150000008046 alkali metal hydrides Chemical class 0.000 description 2
- 150000008044 alkali metal hydroxides Chemical class 0.000 description 2
- 150000001350 alkyl halides Chemical class 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 210000001367 artery Anatomy 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 150000001735 carboxylic acids Chemical class 0.000 description 2
- 239000003054 catalyst Substances 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- DQYBDCGIPTYXML-UHFFFAOYSA-N ethoxyethane;hydrate Chemical compound O.CCOCC DQYBDCGIPTYXML-UHFFFAOYSA-N 0.000 description 2
- HUFSCCBFZSKJMB-XZGRKIOCSA-N ethyl 4-[6-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-3-cyclohexylprop-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]cyclohexane-1-carboxylate Chemical compound C1CC(C(=O)OCC)CCC1=C1C(=O)C2CCC(\C=C\C(O[Si](C)(C)C(C)(C)C)C3CCCCC3)C2C1 HUFSCCBFZSKJMB-XZGRKIOCSA-N 0.000 description 2
- GFSFEZJBVDTARZ-JQIJEIRASA-N ethyl 4-[6-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-3-cyclohexylprop-1-enyl]-3-oxo-2,3a,4,5,6,6a-hexahydro-1h-pentalen-2-yl]-4-hydroxycyclohexane-1-carboxylate Chemical compound C1CC(C(=O)OCC)CCC1(O)C1C(=O)C2CCC(\C=C\C(O[Si](C)(C)C(C)(C)C)C3CCCCC3)C2C1 GFSFEZJBVDTARZ-JQIJEIRASA-N 0.000 description 2
- WKVJIAKSBBEBOP-PKEFVBLTSA-N ethyl 4-[6-[(e)-3-cyclohexyl-3-hydroxyprop-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]cyclohexane-1-carboxylate Chemical compound C1CC(C(=O)OCC)CCC1=C1C(=O)C2CCC(\C=C\C(O)C3CCCCC3)C2C1 WKVJIAKSBBEBOP-PKEFVBLTSA-N 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 125000000623 heterocyclic group Chemical group 0.000 description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-O hydron piperazine Chemical compound [H+].C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-O 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- HGPZXSXOHRATGT-ADPRPDBTSA-N methyl (5e)-5-[6-[(e)-3-[tert-butyl(dimethyl)silyl]oxy-3-cyclohexylprop-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]pentanoate Chemical compound C1CC2C(=O)C(=C/CCCC(=O)OC)/CC2C1\C=C\C(O[Si](C)(C)C(C)(C)C)C1CCCCC1 HGPZXSXOHRATGT-ADPRPDBTSA-N 0.000 description 2
- DUXXFYRHJLFFCN-NXEYJXPOSA-N methyl (5e)-5-[6-[(e)-3-hydroxy-4-phenoxybut-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]pentanoate Chemical compound C1CC2C(=O)C(=C/CCCC(=O)OC)/CC2C1\C=C\C(O)COC1=CC=CC=C1 DUXXFYRHJLFFCN-NXEYJXPOSA-N 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 150000007522 mineralic acids Chemical class 0.000 description 2
- 239000006199 nebulizer Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000012074 organic phase Substances 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- YIBNHAJFJUQSRA-YNNPMVKQSA-N prostaglandin H2 Chemical compound C1[C@@H]2OO[C@H]1[C@H](/C=C/[C@@H](O)CCCCC)[C@H]2C\C=C/CCCC(O)=O YIBNHAJFJUQSRA-YNNPMVKQSA-N 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000001117 sulphuric acid Substances 0.000 description 2
- 235000011149 sulphuric acid Nutrition 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 125000001981 tert-butyldimethylsilyl group Chemical group [H]C([H])([H])[Si]([H])(C([H])([H])[H])[*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- YIORLTKEPLJAQS-UHFFFAOYSA-N (5-oxo-1,2,3,3a,4,6,7,7a-octahydroinden-1-yl) acetate Chemical compound C1C(=O)CCC2C(OC(=O)C)CCC21 YIORLTKEPLJAQS-UHFFFAOYSA-N 0.000 description 1
- SQFRSHOMYSOBIG-OXKWAKDTSA-N (5e)-5-[6-[(e)-3-cyclohexyl-3-hydroxyprop-1-enyl]-3-oxo-1,3a,4,5,6,6a-hexahydropentalen-2-ylidene]pentanoic acid Chemical compound C1CC(C(C(=C/CCCC(O)=O)/C2)=O)C2C1/C=C/C(O)C1CCCCC1 SQFRSHOMYSOBIG-OXKWAKDTSA-N 0.000 description 1
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- AXWLKJWVMMAXBD-UHFFFAOYSA-O 1-butylpiperidin-1-ium Chemical compound CCCC[NH+]1CCCCC1 AXWLKJWVMMAXBD-UHFFFAOYSA-O 0.000 description 1
- NQTSTBMCCAVWOS-UHFFFAOYSA-N 1-dimethoxyphosphoryl-3-phenoxypropan-2-one Chemical compound COP(=O)(OC)CC(=O)COC1=CC=CC=C1 NQTSTBMCCAVWOS-UHFFFAOYSA-N 0.000 description 1
- LPCWDBCEHWHJGX-UHFFFAOYSA-N 1-ethyl-2-methylpiperidine Chemical compound CCN1CCCCC1C LPCWDBCEHWHJGX-UHFFFAOYSA-N 0.000 description 1
- PAMIQIKDUOTOBW-UHFFFAOYSA-O 1-methylpiperidin-1-ium Chemical compound C[NH+]1CCCCC1 PAMIQIKDUOTOBW-UHFFFAOYSA-O 0.000 description 1
- YQOPNAOQGQSUHF-UHFFFAOYSA-N 1-propan-2-ylpyrrolidine Chemical compound CC(C)N1CCCC1 YQOPNAOQGQSUHF-UHFFFAOYSA-N 0.000 description 1
- XEZNGIUYQVAUSS-UHFFFAOYSA-N 18-crown-6 Chemical compound C1COCCOCCOCCOCCOCCO1 XEZNGIUYQVAUSS-UHFFFAOYSA-N 0.000 description 1
- LJDSTRZHPWMDPG-UHFFFAOYSA-N 2-(butylamino)ethanol Chemical compound CCCCNCCO LJDSTRZHPWMDPG-UHFFFAOYSA-N 0.000 description 1
- NNWUEBIEOFQMSS-UHFFFAOYSA-N 2-Methylpiperidine Chemical compound CC1CCCCN1 NNWUEBIEOFQMSS-UHFFFAOYSA-N 0.000 description 1
- MWGATWIBSKHFMR-UHFFFAOYSA-N 2-anilinoethanol Chemical compound OCCNC1=CC=CC=C1 MWGATWIBSKHFMR-UHFFFAOYSA-N 0.000 description 1
- LYGKSUOGJYYSOI-UHFFFAOYSA-N 2-methoxybuta-1,3-diene Chemical compound COC(=C)C=C LYGKSUOGJYYSOI-UHFFFAOYSA-N 0.000 description 1
- OZYXJZDQDFYNKS-UHFFFAOYSA-N 5-methoxy-2,3,3a,4,7,7a-hexahydro-1h-inden-1-ol Chemical compound C1C(OC)=CCC2C(O)CCC21 OZYXJZDQDFYNKS-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical group [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 240000004246 Agave americana Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 101000862089 Clarkia lewisii Glucose-6-phosphate isomerase, cytosolic 1A Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 229940126062 Compound A Drugs 0.000 description 1
- BWLUMTFWVZZZND-UHFFFAOYSA-N Dibenzylamine Chemical compound C=1C=CC=CC=1CNCC1=CC=CC=C1 BWLUMTFWVZZZND-UHFFFAOYSA-N 0.000 description 1
- 239000004150 EU approved colour Substances 0.000 description 1
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 238000003820 Medium-pressure liquid chromatography Methods 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- AKNUHUCEWALCOI-UHFFFAOYSA-N N-ethyldiethanolamine Chemical compound OCCN(CC)CCO AKNUHUCEWALCOI-UHFFFAOYSA-N 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 1
- NQRYJNQNLNOLGT-UHFFFAOYSA-O Piperidinium(1+) Chemical compound C1CC[NH2+]CC1 NQRYJNQNLNOLGT-UHFFFAOYSA-O 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- RWRDLPDLKQPQOW-UHFFFAOYSA-O Pyrrolidinium ion Chemical compound C1CC[NH2+]C1 RWRDLPDLKQPQOW-UHFFFAOYSA-O 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- TXAWWIFWKQIBNO-DHZHZOJOSA-N [4-[(e)-3-cyclohexyl-3-oxoprop-1-enyl]-1,2,3,3a,4,5,6,6a-octahydropentalen-1-yl] acetate Chemical compound C1CC2C(OC(=O)C)CCC2C1\C=C\C(=O)C1CCCCC1 TXAWWIFWKQIBNO-DHZHZOJOSA-N 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 125000003668 acetyloxy group Chemical group [H]C([H])([H])C(=O)O[*] 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 125000004423 acyloxy group Chemical group 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- SMZOGRDCAXLAAR-UHFFFAOYSA-N aluminium isopropoxide Chemical compound [Al+3].CC(C)[O-].CC(C)[O-].CC(C)[O-] SMZOGRDCAXLAAR-UHFFFAOYSA-N 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000002376 aorta thoracic Anatomy 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WGQKYBSKWIADBV-UHFFFAOYSA-O benzylaminium Chemical compound [NH3+]CC1=CC=CC=C1 WGQKYBSKWIADBV-UHFFFAOYSA-O 0.000 description 1
- AZWXAPCAJCYGIA-UHFFFAOYSA-N bis(2-methylpropyl)alumane Chemical compound CC(C)C[AlH]CC(C)C AZWXAPCAJCYGIA-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 125000001589 carboacyl group Chemical group 0.000 description 1
- 150000001721 carbon Chemical group 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000747 cardiac effect Effects 0.000 description 1
- 239000003729 cation exchange resin Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002026 chloroform extract Substances 0.000 description 1
- 229940117975 chromium trioxide Drugs 0.000 description 1
- WGLPBDUCMAPZCE-UHFFFAOYSA-N chromium trioxide Inorganic materials O=[Cr](=O)=O WGLPBDUCMAPZCE-UHFFFAOYSA-N 0.000 description 1
- GAMDZJFZMJECOS-UHFFFAOYSA-N chromium(6+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[Cr+6] GAMDZJFZMJECOS-UHFFFAOYSA-N 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- JNGZXGGOCLZBFB-IVCQMTBJSA-N compound E Chemical compound N([C@@H](C)C(=O)N[C@@H]1C(N(C)C2=CC=CC=C2C(C=2C=CC=CC=2)=N1)=O)C(=O)CC1=CC(F)=CC(F)=C1 JNGZXGGOCLZBFB-IVCQMTBJSA-N 0.000 description 1
- 239000007891 compressed tablet Substances 0.000 description 1
- 230000001595 contractor effect Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- PAFZNILMFXTMIY-UHFFFAOYSA-O cyclohexylammonium Chemical compound [NH3+]C1CCCCC1 PAFZNILMFXTMIY-UHFFFAOYSA-O 0.000 description 1
- NLUNLVTVUDIHFE-UHFFFAOYSA-N cyclooctylcyclooctane Chemical group C1CCCCCCC1C1CCCCCCC1 NLUNLVTVUDIHFE-UHFFFAOYSA-N 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 150000001983 dialkylethers Chemical class 0.000 description 1
- 230000035487 diastolic blood pressure Effects 0.000 description 1
- 239000002027 dichloromethane extract Substances 0.000 description 1
- SIPUZPBQZHNSDW-UHFFFAOYSA-N diisobutylaluminium hydride Substances CC(C)C[Al]CC(C)C SIPUZPBQZHNSDW-UHFFFAOYSA-N 0.000 description 1
- XXBDWLFCJWSEKW-UHFFFAOYSA-N dimethylbenzylamine Chemical compound CN(C)CC1=CC=CC=C1 XXBDWLFCJWSEKW-UHFFFAOYSA-N 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 229960001123 epoprostenol Drugs 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- ZXYAWONOWHSQRU-UHFFFAOYSA-N ethyl 4-oxocyclohexanecarboxylate Chemical compound CCOC(=O)C1CCC(=O)CC1 ZXYAWONOWHSQRU-UHFFFAOYSA-N 0.000 description 1
- MGPSIDGTLFKDEY-UHFFFAOYSA-N ethyl 5-oxohexanoate Chemical compound CCOC(=O)CCCC(C)=O MGPSIDGTLFKDEY-UHFFFAOYSA-N 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
- 229940093471 ethyl oleate Drugs 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 238000001640 fractional crystallisation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- FVIZARNDLVOMSU-UHFFFAOYSA-N ginsenoside K Natural products C1CC(C2(CCC3C(C)(C)C(O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O FVIZARNDLVOMSU-UHFFFAOYSA-N 0.000 description 1
- ZTQSADJAYQOCDD-UHFFFAOYSA-N ginsenoside-Rd2 Natural products C1CC(C2(CCC3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC(C(C(O)C1O)O)OC1COC1OCC(O)C(O)C1O ZTQSADJAYQOCDD-UHFFFAOYSA-N 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002430 hydrocarbons Chemical group 0.000 description 1
- RCBVKBFIWMOMHF-UHFFFAOYSA-L hydroxy-(hydroxy(dioxo)chromio)oxy-dioxochromium;pyridine Chemical compound C1=CC=NC=C1.C1=CC=NC=C1.O[Cr](=O)(=O)O[Cr](O)(=O)=O RCBVKBFIWMOMHF-UHFFFAOYSA-L 0.000 description 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 229910003002 lithium salt Inorganic materials 0.000 description 1
- 159000000002 lithium salts Chemical class 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003975 mesenteric artery Anatomy 0.000 description 1
- RPNNPZHFJPXFQS-UHFFFAOYSA-N methane;rhodium Chemical compound C.[Rh] RPNNPZHFJPXFQS-UHFFFAOYSA-N 0.000 description 1
- 230000003228 microsomal effect Effects 0.000 description 1
- 210000001589 microsome Anatomy 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- YNAVUWVOSKDBBP-UHFFFAOYSA-O morpholinium Chemical compound [H+].C1COCCN1 YNAVUWVOSKDBBP-UHFFFAOYSA-O 0.000 description 1
- DAZXVJBJRMWXJP-UHFFFAOYSA-N n,n-dimethylethylamine Chemical compound CCN(C)C DAZXVJBJRMWXJP-UHFFFAOYSA-N 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- YCBSHDKATAPNIA-UHFFFAOYSA-N non-3-ene Chemical compound CCCCCC=CCC YCBSHDKATAPNIA-UHFFFAOYSA-N 0.000 description 1
- 239000012457 nonaqueous media Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 229940006093 opthalmologic coloring agent diagnostic Drugs 0.000 description 1
- 150000002895 organic esters Chemical class 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- WEXRUCMBJFQVBZ-UHFFFAOYSA-N pentobarbital Chemical compound CCCC(C)C1(CC)C(=O)NC(=O)NC1=O WEXRUCMBJFQVBZ-UHFFFAOYSA-N 0.000 description 1
- 229960001412 pentobarbital Drugs 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 150000003174 prostaglandin I2 derivatives Chemical class 0.000 description 1
- LVTJOONKWUXEFR-FZRMHRINSA-N protoneodioscin Natural products O(C[C@@H](CC[C@]1(O)[C@H](C)[C@@H]2[C@]3(C)[C@H]([C@H]4[C@@H]([C@]5(C)C(=CC4)C[C@@H](O[C@@H]4[C@H](O[C@H]6[C@@H](O)[C@@H](O)[C@@H](O)[C@H](C)O6)[C@@H](O)[C@H](O[C@H]6[C@@H](O)[C@@H](O)[C@@H](O)[C@H](C)O6)[C@H](CO)O4)CC5)CC3)C[C@@H]2O1)C)[C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](CO)O1 LVTJOONKWUXEFR-FZRMHRINSA-N 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical compound [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000012258 stirred mixture Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 125000000037 tert-butyldiphenylsilyl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1[Si]([H])([*]C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
- XWVKTOHUMPLABF-UHFFFAOYSA-N thallium(3+) Chemical class [Tl+3] XWVKTOHUMPLABF-UHFFFAOYSA-N 0.000 description 1
- DSNBHJFQCNUKMA-SCKDECHMSA-N thromboxane A2 Chemical compound OC(=O)CCC\C=C/C[C@@H]1[C@@H](/C=C/[C@@H](O)CCCCC)O[C@@H]2O[C@H]1C2 DSNBHJFQCNUKMA-SCKDECHMSA-N 0.000 description 1
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 1
- 125000004665 trialkylsilyl group Chemical group 0.000 description 1
- ZMANZCXQSJIPKH-UHFFFAOYSA-O triethylammonium ion Chemical compound CC[NH+](CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-O 0.000 description 1
- 125000002023 trifluoromethyl group Chemical group FC(F)(F)* 0.000 description 1
- RKBCYCFRFCNLTO-UHFFFAOYSA-N triisopropylamine Chemical compound CC(C)N(C(C)C)C(C)C RKBCYCFRFCNLTO-UHFFFAOYSA-N 0.000 description 1
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 1
- 125000000026 trimethylsilyl group Chemical group [H]C([H])([H])[Si]([*])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- YFTHZRPMJXBUME-UHFFFAOYSA-N tripropylamine Chemical compound CCCN(CCC)CCC YFTHZRPMJXBUME-UHFFFAOYSA-N 0.000 description 1
- DRDCQJADRSJFFD-UHFFFAOYSA-N tris-hydroxymethyl-methyl-ammonium Chemical compound OC[N+](C)(CO)CO DRDCQJADRSJFFD-UHFFFAOYSA-N 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D317/00—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms
- C07D317/08—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3
- C07D317/72—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 spiro-condensed with carbocyclic rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C405/00—Compounds containing a five-membered ring having two side-chains in ortho position to each other, and having oxygen atoms directly attached to the ring in ortho position to one of the side-chains, one side-chain containing, not directly attached to the ring, a carbon atom having three bonds to hetero atoms with at the most one bond to halogen, and the other side-chain having oxygen atoms attached in gamma-position to the ring, e.g. prostaglandins ; Analogues or derivatives thereof
- C07C405/005—Analogues or derivatives having the five membered ring replaced by other rings
- C07C405/0075—Analogues or derivatives having the five membered ring replaced by other rings having the side-chains or their analogues or derivatives attached to a condensed ring system
- C07C405/0083—Analogues or derivatives having the five membered ring replaced by other rings having the side-chains or their analogues or derivatives attached to a condensed ring system which is only ortho or peri condensed, e.g. carbacyclins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C43/00—Ethers; Compounds having groups, groups or groups
- C07C43/02—Ethers
- C07C43/18—Ethers having an ether-oxygen atom bound to a carbon atom of a ring other than a six-membered aromatic ring
- C07C43/196—Ethers having an ether-oxygen atom bound to a carbon atom of a ring other than a six-membered aromatic ring containing hydroxy or O-metal groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/56—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds from heterocyclic compounds
- C07C45/57—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds from heterocyclic compounds with oxygen as the only heteroatom
- C07C45/59—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds from heterocyclic compounds with oxygen as the only heteroatom in five-membered rings
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
- C07C45/61—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups
- C07C45/67—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton
- C07C45/68—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by increase in the number of carbon atoms
- C07C45/69—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by isomerisation; by change of size of the carbon skeleton by increase in the number of carbon atoms by addition to carbon-to-carbon double or triple bonds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
- C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
- C07C49/703—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups
- C07C49/743—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups having unsaturation outside the rings, e.g. humulones, lupulones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
- C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
- C07C49/753—Unsaturated compounds containing a keto groups being part of a ring containing ether groups, groups, groups, or groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic Table
- C07F7/02—Silicon compounds
- C07F7/08—Compounds having one or more C—Si linkages
- C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
- C07F7/1804—Compounds having Si-O-C linkages
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/28—Phosphorus compounds with one or more P—C bonds
- C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
- C07F9/40—Esters thereof
- C07F9/4003—Esters thereof the acid moiety containing a substituent or a structure which is considered as characteristic
- C07F9/4006—Esters of acyclic acids which can have further substituents on alkyl
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Prostaglandin analogues: <IMAGE> (wherein R<1> represents hydrogen or alkyl group, Y<1> represents carbonyl hydroxymethylene, ---- represents a single or double bond, A<1> represents an alkylene linkage, R<2> represents hydrogen methyl or ethyl and R<3> represents hydrogen or else R<2> and R<3> form an alkylene linkage such that the symbols A<1>, R<2> and R<3>, together with the carbon atoms through which they are connected, form a cycloalkyl ring of 6,7 or 8 carbon atoms, optionally bearing one or two methyl or ethyl substituents, X<1> represents ethylene or trans-vinylene Y<2> represents carbonyl or hydroxymethylene and either (i) A<2> represents an alkylene chain Z<1> represents a direct bond, oxygen or sulphur and R<4> represents alkyl, optionally substituted by cycloalkyl atoms, or R<4> represents cycloalkyl or R<4> represents phenyl optionally substituted by halogen, a trifluoromethyl alkyl or alkoxy or (ii) A<2> and Z<1> both represent direct bonds and R<4> represents alkyl optionally substituted by cycloalkyl, or R<4> represents cycloalkyl and cyclodextrin clathrates thereof and, when R<1> represents a hydrogen atom, non-toxic salts thereof, possess useful pharmacological properties. The compounds are more stable than PGl2 and have utility in regard to hypertension, thrombosis, arteriosclerosis, athersclerosis, angina and myocardial infarction- intermediates including dialkyl phosphonates useful in the preparation of other prostaglandins are also claimed.
Description
SPECIFICATION
New prostaglandin analogues
This invention relates to new prostaglandin 12 analogues, to processes for their preparation, to pharmaceutical compositions containing them, and tokes intermediates which can be used in their preparation.
Prostaglandin 12 (otherwise known as PGIz or prostacyclin) is a physiologically active natural substance having the formula shown in Figure I of the drawings assembled at the end of this specification and its chemical name is (5Z,1 3E)-(9S,1 1R,1 5S)-6,9-epoxy-1 1,1 5-dihydroxyprosta- 5,13-dienoic acid [Nature, 263, 663 (1976), Prostaglandins,12,685 (1976), ibid, 12, 915 (1976), ibid, 13,3 (1977), ibid, 13, 375 (1977) and Chemical and Engineering News, Dec. 20, 17 (1976)].
It is well known that PGl2 can be prepared by incubation of prostaglandin G2 (PGG2) or prostaglandin H2 (PG H2) with microsomal fractions prepared from thoracic aorta of swine, mesenteric artery of swine, rabbit aorta or the stomach fundus of rats. PGl2 has a strong relaxing activity on the artery and some other kinds of smooth muscle. Furthermore, PGl2 strongly inhibits arachidonic acidinduced blood platelet aggregation of the human.
Taking into consideration that thromboxane A2, prepared by incubation of PGG2 or PGH2 with blood platelet microsome, has a contracting activity on the artery and an aggregating activity on blood platelets, the properties of PGl2 heretofore mentioned show that PGI2 fulfils a very important physiological part in a living body. PGl2 may be useful in the treatment of arteriosclerosis, atherosclerosis, cardiac failure, thrombosis, hypertension, angina or asthma.
Natural PGl2 is so unstable (being deactivated in a buffer solution at pH 7.6 after 20 minutes at 220C, or after 10 minutes at 370C) that application of PGl2 for medical purposes is difficult.
Widespread investigations have been carried out in order to discover processes for the chemical preparation of more stable analogues of PIG 12, and their products possessing the pharmacological properties of the 'natural' PGl2 or one or more of such properties to an, enhanced degree, or hitherto unknown pharmacological properties. As a result of extensive research and experimentation it has been discovered that in certain analogues of PGl2 and derivatives thereof the properties of the 'natural' PGl2 are, in some aspects of its activities, improved or modified.
The present invention accordingly provides new prostaglandin analogues of the general formula shown in Figure II [wherein R' represents a hydrogen atom or a straight- or branched-chain alkyl group preferably containing from 1 to 6 carbon atoms, Y' represents a carbonyl or hydroxymethylene group, - - - - - represents a single or double bond, A' represents an alkylene linkage containing 2 or 3 carbon atoms and optionally bearing a methyl or ethyl substituent, R2 represents a hydrogen atom or a methyl
or ethyl group and R3 represents a hydrogen atom or else R2 and R3 form an alkylene linkage containing 2 or 3 carbon atoms, optionally bearing a methyl or ethyl substituent, such that the symbols Al, R2 and
R3, together with the carbon atoms through which they are connected, form a cycloalkyl ring of 6, 7 or 8 carbon atoms, optionally bearing one or two methyl or ethyl substituents, X1 represents an ethylene or trans-vinylene group, Y2 represents a carbonyl or hydroxymethylene group, and either (i) A2 represents a straight- or branched-alkylene chain containing from 1 to 3 carbon atoms, Z' represents a direct bond or an oxygen or sulphur atom, and R4 represents a straight- or branched-chain alkyl group containing from 1 to 1 2 carbon atoms, optionally substituted by a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a phenyl group optionally substituted by a halogen (e.g. chlorine, bromine, fluorine or iodine) atom or by a trifluoromethyl group or by a straight- or branched-chain alkyl or alkoxy group containing from 1 to 6 carbon atoms, or (ii) A2 and Z' both represent direct bonds and R4 represents a straight- or branchedchain alkyl group containing from 1 to 12 carbon atoms, optionally substituted by a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a cycloalkyl group containing from 3 to 8 carbon atoms] as well as the cyclodextrin clathrates and, when R1 represents a hydrogen atom, non-toxic salts thereof.
In this specification, whenever reference is made to compounds of the formula shown in Figure II, it is intended to refer also to the said cyclodextrin clathrates and non-toxic salts thereof whenever the context so permits.
As will be apparent to those skilled in the art, the compounds of the formula shown in Figure II have at least three centres of chirality, these three centres of chirality being at the carbon atoms in positions 8, 9 and 12. In addition to these three centres of chirality, a further centre of chirality will occur when Y' or Y2 represents a hydroxymethylene group and still further centres of chirality may occur in the groups A', R' and R2 and in positions 2, 5 and 6. The presence of centres of chirality, as is well known, leads to the existence of isomerism. However, the compounds of the formula shown in
Figure II all have such a configuration that the hydrogen atoms attached to the bridgehead carbon atom in positions 8 and 9 are cis with respect to each other.Accordingly, all isomers of the formula shown in Figure II, and mixtures thereof, which have those hydrogen atoms, attached to the bridgehead carbon atoms in positions 8 and 9, in the cis-configuration are within the scope of the present invention. Preferably the hydrogen atoms attached to the 8 and 9 positions are in the same configurations as those in PIG 12, viz. beta and beta respectively. Particularly preferred compounds are those wherein the sidechain attached to the 12-position is cis with respect to the said hydrogen atoms attached to the bridgehead carbon atoms in positions 8 and 9.
Those skilled in the art will also appreciate that # can be in the (E) or the (Z)-configuration when it represents a vinylene group,
According to a feature of the present invention compounds of the formula shown in Figure II in the (5Z)-configuration can be prepared by the irradiation of corresponding compounds of the formula shown in Figure II in the (SE)-configuration. Usually ultra-violet radiation is used, and it is convenient to use the 254nm rays emitted by a low pressure mercury vapour lamp.
Compounds of the formula shown in Figure II which are of especial importance are those of the general formula shown in Figure lil (wherein A1, A2, R1, R2, R3, R4, - - - - -, X1, y', y2 and Z' are as hereinbefore defined) and also their enantiomers and non-toxic salts and cyclodextrin clathrates thereof.
Especially preferred classes of compounds of the formula shown in Figure II are those which exhibit one or more of the following features:- (i) A' represents an ethylene group;
(ii) A2 represents a direct bond or a methylene group;
(iii) R4 represents a straight- or branched-chain alkyl group containing from 1 to 12, preferably from 4 to 8, carbon atoms, more especially a pentyl group, or represents a cycloalkyl group containing from 3 to 8 carbon atoms, more especially a cyclohexyl group, or a phenyl group;
(iv) R' represents a hydrogen atom or a methyl or ethyl group;
(v) R2 represents a hydrogen atom or a methyl group;
(vi) R3 represents a hydrogen atom;
(vii) Al, R2 and R3, together with the carbon atoms through which they are connected, form a cyclohexyl ring; (viii) # represents a vinylene group;; (ix) X1 represents a trans-vinylene group;
(x) Y2 represents a hydroxymethylene group;
(xi) Z' represents a direct bond or an oxygen atom.
Individual compounds of particular importance include the following: (+)-methyl (5E,1 3E)-(9S,1 5S)-6a-oxo-6,9-methano-15-cyclohexyl-15- hydroxy-1 6,1 7,18,1 9,20-pentanorprosta-S, 1 3-dienoate; A (+)-(5E, 1 3E)-(9S, 1 5S)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy- 16,17,18,1 9,20-pentanorprosta-5,1 3-dienoic acid; B (+)-[mixture of (5E) and (5Z)],(13E)-(9S,15S)-6a-oxo-6,9-methano-15- cyclohexyl-1 S-hydrnxy-1 6,17,18,1 9,20-pentanorprosta-S,13- dienoic acid; C (+)-methyl (5E,1 3E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-
methano-15-hydroxyprosta-5,13-dienoate;D (+)-(5E, 1 3E)-(9S),[mixture of (15R) and (15S)]-6a-oxo-6,9-methano-15- hydroxyprosta-5,13-dienoic acid; E (+)-(5Z,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 S- hydroxyprosta-5,1 3-dienoic acid; (+)-methyl (5Z,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-
methano-15-hydroxyprosta-5,13-dienoate; G (+)-methyl (5E,1 3E)-(9S),[mixture of (1 SR) and (1 5S)]-6a-oxo-6,9- methano-l 5-cyclohexyl-l 5-hydroxy-l 6,1 7,18,19,20- pentanorprosta-5,13-dienoate;H (+)-(5E,1 3E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 5- cyclohexyl-1 S-hydroxy-1 6,17,18,1 9,20-pentanorprosta-S,1 3- dienoic acid; (+)-(5E,1 3E)-(6aS,9S),[mixture of (1 SR) and (15S)]-6a,15-dihydroxy-6,9- methano-1 5-cyclohexyl- 16,17,18,19,20-pentanorprosta-5,13- dienoic acid; J ()-ethyl (13E)-(9S,15S)-6a-oxo-2,5-ethano-6,9-methano-15-hydroxy-
15-cyclohexyl-16,17,18,19,20-pentanorprosta-5,13-dienoate; K (+)-methyl (5E, 1 3E)-(9S, 1 5R)-6a-oxo-6,9-methano-15-cyclohexyl-15- hydroxy-1 6,1 7,1 8,1 9,20-pentanorprosta-S,1 3-dienoate; L (+)-(5E, 13E)-(9S,1 5R)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy- 16,17.18,1 9,20-pentanorprosta-5, 13-dienoic acid M (+)-ethyl [mixture of (5E) and (5Z)],(1 3E)-(9S,1 SR)-6a-oxo-6,9-methano 5-methyl-l 6-cyclohexyl-l 5-hydroxy-l 6,17,18,19.20- pentanorprosta-5,13-dienoate; N ()-ethyl (13E)-(9S,15R)-6a-oxo-2,5-ethano-6,9-methano-15-hydroxy-
15-cyclohexyl-16,17,18,19,20-pentanorprosta-5,13-dienoate;O (+)-methyl (5E,1 3E)-(9S,1 SS)-6a-oxo-6,9-methano-1 S-hydroxy-1 6- phenoxy-l 7,18,1 9,20-tetranorprosta-5, 13-dienoate; P (1)-methyl (5E, 1 3E)-(9S, 1 5R)-6a-oxo-6,9-m etha no- 1 5-hyd roxy-l 6- phenoxy-1 7,1 8,1 9,20-tetranorprosta-5, 1 3-dienoate; and Q (1)-methyl (5E,1 3E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9- methano-l 5-hydroxy-l 6-phenoxy-l 7,18,1 9,20-tetranorprosta- 5,13-dienoate. R
The letters A to R are assigned to the compound for easy reference later in the specification.
The compounds of the formula shown in Figure II and cyclodextrin clathrates and non-toxic salts thereof possess valuable pharmacological properties, for example, properties typical of the related series of natural products known as prostaglandins.
In laboratory screening tests the compounds produced an inhibition of collagen-induced p- thromboglobulin release in vitro in human blood, as shown in Table I below, which indicates the concentrations of the test compounds (ED50) in 84g/ml which produced 50% inhibition of the ,B- thromboglobulin release.
In other laboratory tests, compound B produced a 25% fall in diastolic blood pressure in rats (anaesthetised with pentobarbitone) when administered intravenously at a rate of 45,ug/kg animal body weight.
These results are indicated of utility in the prevention or treatment of conditions such as hypertension, thrombosis, and possibly arteriosclerosis, atherosclerosis, and cardiac conditions such as angina and myocardial infarction.
This utiiity is enhanced by the fact that the compounds of the formula shown in Figure II are far more stable than natural PGl2 and so they and their pharmaceutical compositions may be manipulated, stored and administered relativeiy easily.
Table I
Test compound EDsOyg/ml B 5 E 34 F less than 17 I less than 17 According to a feature of the present invention, compounds of the formula shown in Figure II wherein Y2 represents a hydroxymethylene group are prepared by the acid hydrolysis of compounds of the general formula shown in Figure IV wherein A1, A2, R1, R2, R3, R4, - - - - -, X1, Y1 and Z1 are as hereinbefore defined and R5 represents a suitable acid-labile protecting group.
This process is particularly applicable to the preparation of compounds of the formula shown in Figure II wherein R' represents an alkyl group, - - - - - represents a vinylene group, X' represents a trans-vinyiene group, Y1 represents a carbonyl group, and Y2 represents a hydroxymethylene group, that is to say the preparation of compounds of the general formula shown in Figure V (wherein Al, A2,
R2, R3, R4 and Z' are as hereinbefore defined and R6 represents a straight- or branched-chain alkyl group preferably containing from 1 to 6 carbon atoms) by the acid hydrolysis of compounds of the general formula shown in Figure VI (wherein AX, A2, R2, R3, R4, R5, R5 and Z1 are as hereinbefore defined), within the formula shown in Figure IV.
Hydrolysis of compounds of the formula shown in Figure IV is generally effected in mild acidic conditions, for example by treatment with an aqueous inorganic acid, e.g. dilute hydrochloric acid or a catalytic quantity of perchloric acid, or an aqueous organic acid, for example aqueous acetic acid, e.g.
5080% v/v aqueous acetic acid, preferably in the presence of an inert organic solvent, for example a lower alkanol, e.g. ethanol, or an ether, e.g. diethyl ether or tetrahydrofuran, and optionally in the presence of a cation exchange resin, e.g. Dowex AG50W-X8 H+ resin. The hydrolysis is generally carried out at temperatures from OOC to 100 C; when dilute hydrochloric acid is used, at from 400 to 800 C, preferably from 500 to 600C; when a catalytic quantity of perchloric acid is used, at from 0 to 400C, preferably from 1 50 to 250 C; and when aqueous acetic acid is used, at from 0 to 800C, preferably from 350 to 500C.
Suitable acid labile protecting groups represented by R5 are those which are easily removed by acid hydrolysis and which do not cause side reactions, e.g. a 2-tetrahydropyranyl group unsubstituted or substituted by at least one straight- or branched-chain alkyl group containing from 1 to 4 carbon atoms, or a 2-tetrahydrofuranyl group, or a tert-butyldiphenylsilyl group, or a trialkylsilyl group of the general formula.
SiR7R8R9 VII (wherein R9 and R9, which may the same or different, each represents a methyl or ethyl group and R7 represents a straight- or branched-chain alkyl group containing from 1 to 4 carbon atoms), e.g. a trimethylsilyl, triethylsilyl, dimethylisopropylsilyl or tert-butyldimethylsilyl group, or a 1 -alkoxyalkyl group of the general formula: --CH(CH2R1O)OR1' VIII (wherein R'O represents a hydrogen atom or a straight- or branched-chain alkyl group containing from
1 to 4 carbon atoms and R'1 represents a straight- or branched-chain alkyl group containing from 1 to 4 carbon atoms) e.g. a 1-ethoxyethyl group.
Preferably R5 represents a tert-butyl-dimethylsilyl group.
Compounds of the formula shown in Figure II may be prepared from other compounds of the formula shown in Figure II. Thus, according to a further feature of the present invention, the compounds of the formula sh shown in Figure V are converted to compounds of the formula shown in Figure II, wherein A1, A2, Ra, R2, R3, R4, . - - - -, Xr, yr, y2 and Z1 are as hereinbefore defined but wherein one or more of the symbols R', - - - - -, X1, Y' and Y2 have the following significances:- (a) R' represents a hydrogen atom; (b) -- -- - - represents an ethylene group; (c) X' represents an ethylene group;
(d) Y' represents a hydroxymethylene group;
(e) Y2 represents a carbonyl group; or to salts of compounds of the formula shown in Figure II wherein Ra represents a hydrogen atom, or to cyclodextrin clathrates of the compounds of the formula shown in Figure II, by the application or adaptation of one or more known methods of preparing acids from esters of preparing alcohols from ketones, of preparing ketones from alcohols, of reducing carbon-carbon double bonds, of preparing salts from acids, or of preparing cyclodextrin clathrates. Furthermore, esters of the formula shown in
Figure II wherein R1 represents an alkyl group can be prepared by the esterification of corresponding carboxylic acids of the formula shown in Figure II wherein R1 represents a hydrogen atom.
Thus, (1) compounds of the formula shown in Figure II in which R1 represents a hydrogen atom may be prepared by the hydrolysis of the corresponding compounds of the formula shown in Figure II wherein R1 represents an alkyl group by hydrolysis, for example with an aqueous alkali (e.g. lithium hydroxide, sodium hydroxide or potassium hydroxide), followed by treatment with dilute acid, e.g.
hydrochloric acid, to generate the desired carboxylic acid product from the solution of alkali metal salt produced thereby.
(2) Compounds of the formula shown in Figure II wherein - - - - -, X1, Y1 and Y2 do not simultaneously represent two vinylene groups and two carbonyl groups respectively (hereinafter referred to as "compounds of formula Ila") are prepared by the reduction of compounds of the formula shown in Figure II wherein - - - - -, Xt, Y1 and Y2 do not simultaneously represent two ethylene groups and two hydroxymethylene groups respectively (hereinafter referred to as "compounds of formula llb").
Thus:
(2) (a) Compounds of formula Ila wherein - - - - - and X1 represent ethylene or vinylene groups and one or both of Y' and Y2 represents a hydroxymethylene group are prepared by reduction of the corresponding compounds of formula llb wherein - - - - - and X' each represents an ethylene or vinylene group and one or both of Y1 and Y2 represents carbonyl, using means and conditions capable of reducing carbonyl groups to hydroxymethylene groups without affecting carbon-carbon double bonds. The reduction is preferably effected by a metal borohydride or a metal alkylborohydride (e.g.
sodium borohydride or potassium borohydride or lithium tri-sec-butylborohydride), usually in an aqueous, alcoholic or aqueous alcoholic medium and at between -400 and +300 C., preferably between --50 and +150C., optionally in the presence of a base, for example an alkali metal hydroxide (e.g. aqueous sodium hydroxide or aqueous potassium hydroxide) when a metal borohydride is employed or, especially when potassium borohydride is employed, in aqueous or aqueous alcoholic conditions buffered at a pH of from pH 7 to pH 9, e.g. at pH 8 (e.g. by the addition of aqueous citric acid solution), or, when a metal aikylborohydride is employed, in an ethereal medium (e.g. tetrahydrofuran) at between -780 and OOC.
Alternatively the reduction is carried out by reaction with aluminium isopropoxide, in the presence of isopropanol, preferably as the solvent medium, at an elevated temperature, advantageously at the reflux temperature of the reaction mixture.
(2) (b) Compounds of formula Ila wherein one or both of - - - - - and X1 represents an ethylene group and Y' and Y2 each represents a carbonyl or hydroxymethylene group are prepared by reduction of the corresponding compounds of formula llb wherein one or both of - - - - - and X1 represents a vinylene group and Y' and Y2 each represents a carbonyl or hydroxymethylene group, with means and in conditions capable of reducing carbon-carbon double bonds without affecting carbonyl groups. The reduction is preferably effected by hydrogenation in the presence of a hydrogenation catalyst, for example rhodium on charcoal, in the presence of an inert organic solvent, for example a lower alkanol, e.g. ethanol, generally at ambient temperature and elevated pressure, e.g. at a hydrogen pressure of 1 5 kilograms per square centimetre.
(2) (c) Compounds of formula Ila wherein - - - -- - and X' both represent ethylene groups and Y' and Y2 both represent hydroxy-methylene groups are prepared by reduction of corresponding compounds of formula llb with means and in conditions capable of reducing any carbonyl groups represent to hydroxymethylene groups and any vinylene groups present to ethylene groups. The reduction is preferably effected by hydrogenation in the presence of a hydrogenation catalyst, for example palladium on charcoal, in the presence of an inert organic solvent, for example a lower alkanol, e.g. ethanol, preferably at an elevated pressure, e.g. at a hydrogen pressure of 1 5 kilograms per square centimetre.
(3) Compounds of the formula shown in Figure II wherein one or both of Y1 and Y2 represents a carbonyl group may be prepared by oxidation of the corresponding compounds of the formula shown in
Figure II wherein one or both of Y1 andY2 represents a hydroxymethylene group with means and in conditions capable of oxidising hydroxymethylene groups to form carbonyl groups without affecting the rest of the molecule. The oxidation is preferably effected by means of pyridinium chlorochromate, preferably in dichloromethane, or by means of pyridinium dichromate, preferably in dimethylformamide or dichloromethane, at or near room temperature, or by means of a solution prepared from chromium trioxide, sulphuric acid and water, preferably in the presence of acetone and at or below room temperature.
(4) By the term "non-toxic salts" is meant salts the cations of which are relatively innocuous to the animal organism when used in therapeutic does so that the beneficial properties of the parent compound of the formula shown in Figure II are not vitiated by side-effects ascribable to those cations.
Preferably, the salts are water-soluble. Suitable salts include the alkali metal, e.g. sodium or potassium, and ammonium salts and pharmaceutically-acceptable (i.e. non-toxic) amine salts.
Amines suitable for forming such salts with carboxylic acids are well known and include, for example, amines derived in theory by the replacement of one or more of the hydrogen atoms of ammonia by groups, which may be the same or different when more than one hydrogen atom is replaced, selected from alkyl groups containing from 1 to 6 carbon atoms, hydroxyalkyl groups containing 2 or 3 carbon atoms, cycloalkyl groups containing from 3 to 6 carbon atoms phenyl groups, phenylalkyl groups containing from 7 to 11 carbon atoms and phenylalkyl groups containing from 7 to 1 5 carbon atoms wherein the alkyl moieties are substituted by hydroxy groups. The phenyl groups and
phenyl moieties of such phenylalkyl groups may be unsubstituted or substituted by one or two alkyl
groups containing from 1 to 6 carbon atoms.Suitable amines also include those derived in theory by the replacement of two of the hydrogen atoms of ammonia by a hydrocarbon chain, which may be
interrupted by nitrogen, oxygen or sulphur atoms, to form, together with the nitrogen atom of ammonia to which its terminal groups are attached, a five- or six-membered nitrogen-containing heterocyclic ring, which heterocyclic ring may be unsubstituted or substituted by one or two alkyl groups containing from 1 to 6 carbon atoms.Examples of suitable amine cations include mono- di- and tri-methylammonium,
mono-, di- and tri-ethylammonium, mono-, di- and tri-propylammonium, mono- di- and tri-isopropyl
ammonium, ethyldimethylammonium, mono-, bis- and tris-(2-hydroxyethyl)ammonium, ethylbis(2
hydroxyethyl)ammonium, butylmono-(2-hydroxyethyl)ammonium, tris(hydroxymethyl)methyl- ammonium, cyclohexylammonium, benzylammonium, benzyldimethylammonium, dibenzylammonium, phenyl-2-hydroxyethylammonium, piperidinium, morpholinium, pyrrolidinium, piperazinium, 1 -methylpiperidinium, 4-ethylmorpholiniu m, 1 -isopropylpyrrolidinium, 1 ,4-dimethyl-.
piperazinium, 1 -butylpiperidinium, 2-methylpiperidinium and 1 -ethyl-2-methylpiperidinium.
The non-toxic salts may be prepared from parent compounds of the formula shown in Figure II by known methods, for example by reaction of stoichiometric quantities of compounds of the formula shown in Figure II (wherein Ra represents a hydrogen atom) and the appropriate base, e.g. an alkali metal hydroxide or carbonate, ammonia or an amine, in a suitable solvent which is preferably water in the case of the preparation of alkali metal salts and water or isopropanol in the case of amine salts. The salts may be isolated by lyophilisation of the solution or, if sufficiently insoluble in the reaction medium, by filtration, if necessary after removal of part of the solvent.
As well as being useful in themselves as pharmaceutically useful compounds, salts of the compounds of the formula shown in Figure li wherein R' represents a hydrogen atom are useful for the purpose of purification of the parent acids of the formula shown in Figure II, for example by exploitation of the solubility differences between the salts and the parent acids in water and in organic solvents, by techniques well known to those skilled in the art. The parent acids of the formula shown in Figure II can be regenerated from their salts by known methods, for example by treatment with a mineral acid, e.g.
dilute hydrochloric acid.
(5) Cyclodextrin clathrates of the prostaglandin analogues of the formula shown in Figure II may be prepared by dissolving the cyclodextrin in water or an organic solvent which is miscible with water, in the presence of triethylamine, and adding to the solution the prostaglandin analogue in a water
miscible organic solvent. The mixture is then heated and the desired cyciodextrin clathrate product
isolated by concentrating the mixture under reduced pressure or by cooling and separating the product by filtration or decantation. The ratio of organic solvent to water may be varied according to the solubilities of the starting materials and products.Preferably the temperature is not allowed to exceed 700C during the preparation of the cyclodextrin clathrates. a-, P- or y-Cyclodextnns or mixtures thereof may be used in the preparation of the cyclodextrin clathrates. Conversion into their cyclodextrin clathrates serves to increase the stability of the prostaglandin analogues.
(6) Compounds of the formula shown in Figure II wherein R' represents an alkyl group may be prepared by the reaction of a corresponding carboxylic acid of the formula shown in Figure II in which R' represents a hydrogen atom with an alcohol of the general formula:: R6OH IX (wherein R6 is as hereinbefore defined), an excess of which may be employed as solvent medium, in the presence of an inorganic acid, e.g. hydrochloric acid or sulphuric acid, preferably at a temperature between 500 and 1 600 C, and advantageously at the reflux temperature of the reaction mixture, or, where R6 can be represented by the formula --CHR'2R'3 [wherein the symbols R12 and R'3 are identical or different and each represents an alkyl group (the total number of carbon atoms in the two groups R12 and R'3 being preferably at most 11) or, preferably, a hydrogen atom], with a diazoalkane of the general formula:: R12R13C=N2 X (wherein R12 and R'3 are as hereinbeforn defined) in an inert organic solvent medium, preferably a dialkyl ether (e.g. diethyl ether), preferably at ambient temperature. Alternatively, a silver salt of such a carboxylic acid of the formula shown in Figure II can be reacted with an alkyl halide of the general formula:: R6z2 Xl wherein Z2 represents a halogen atom and R6 is as hereinbefore defined, optionally in the presence of an inert organic solvent such as an aromatic hydrocarbon e.g. benzene) at an elevated temperature and advantageously at the reflux temperature of the reaction mixture, or a sodium salt of a said carboxylic acid of the formula shown in Figure II can be reacted with a said alkyl halide in a polar solvent, such as hexamethylphosphotriamide, preferably at room temperature.
By the term "known methods" as used in this specification is meant methods heretofore used or described in the literature.
As will be readily appreciated by those skilled in the art, the compounds of the formula shown in
Figure II, including their isomers arising from the aforementioned centres of chirality, may be separated by the application or adaptation of known methods. For example, diastereoisomeric forms may be separated by chromatography using selective adsorption from solution or from the vapour phase onto suitable adsorbents, and enantiomeric forms of compounds of the formula shown in Figure II wherein
R' represents a hydrogen atom may be separated by formation of salts with an optically active base, followed by separation of the obtained pair of diastereoisomers by, for example, fractional crystallisation from a suitable solvent system, followed by separate regeneration of the enantiomeric acids.
Compounds of the formula shown in Figure IV may be prepared by the application or adaptation of known methods, for example methods illustrated in the description in the following Reference
Examples for the preparation of compounds of the formula shown in Figure VI.
An essential feature of any such multistage process for the preparation of compounds of the formula shown in Figure II is the formation of the bicyclo[3,3,0]octane skeleton, and Reference
Example 5 illustrates a novel process for the preparation of key intermediates containing that double ring system.
Thus, according to a feature of the present invention, key intermediates of the general formula shown in Figure XII wherein R'4 represents an acyl group, more especially a lower alkanoyl (e.g. acetyl) group are prepared by the oxidation of compounds of the general formula shown in Figure XIII wherein R'4 is as hereinbefore defined.
Preferably the oxidation is carried out by reaction with a thallium (III) salt, e.g. thallic nitrate, preferably in an organic acid medium, more especially glacial acetic acid, and preferably at an elevated temperature, e.g. 40--1200C.
As an alternative, the oxidation is carried out by means of hydrogen peroxide in the presence of selenium dioxide. Conveniently the reaction may be carried out in tert-butanol as a solvent, and at an elevated temperature, e.g. 800 C.
The resulting compounds of the formula shown in Figure XII can then be elaborated to form compounds of the formula shown in Figure II by the application or adaptation of methods illustrated in the following Examples and Reference Examples.
Another important feature of the multistage process for the preparation of compounds of the formula shown in Figure II is the formation of the side-chain in the 12-position, and Reference Example 22 illustrates the use of a novel reagent for the formation of a side-chain from a formyl group in the 12position.
Thus according to a further feature of the present invention, there are provided salts of dialkylphosphonates, of the general formula: [M']+ [(R'5O)2P(O)CHCO(CH2)4CH3]- XIV (wherein M' represents an alkali metal atom such as a lithium or, more particularly, sodium atom, and
R'5 represents an alkyl group of from 1 to 4 carbon atoms, preferably a methyl group) in their solid, isolated, form.
According to a feature of the present invention, compounds of formula XIV may be used in the application or adaptation of known methods for the conversion of formyl groups to 3-oxooct-1 -enyl sidechains, for example, according to a feature of the invention, there is provided a process for the preparation of a compound of the general formula shown in Figure XV (wherein R16 represents a protected oxygen function such as an acyloxy, e.g. acetoxy, group or an ethylenedioxy group) by the reaction of a compound of the general formula shown in Figure XVI (wherein R16 is as hereinbefore defined) with a compound of the general formula XIV (wherein M' and R15 are as hereinbefore defined), preferably in an inert organic solvent such as an ether (e.g. tetrahydrofuran) and preferably at or near room temperature, characterised in that the said compound of formula XIV is provided in its solid, isolated, form and not prepared in situ.
In the past, reagents such as those of formula XIV have been generated in situ by the action of a strong base such as an alkali metal hydride, e.g. sodium hydride, or an alkali metal alkyl, e.g. butyl lithium, on the parent dialkylphosphonates, usually in an inert organic solvent such as an ether, e.g.
tetrahydrofuran.
Compounds of formula XIV may be prepared in their solid, isolated, form by the action of a strong base such as an alkali metal hydride, e.g. sodium hydride, or an alkali metal alkyl, e.g. butyl lithium, on phosphonates of the general formula: (R'5O)2P(O)CHCO(CH2)4CH3 XVII (wherein R15 is as hereinbefore defined) in an inert organic solvent such as an ether, e.g. tetrahydrofuran, at or near room temperature and under an inert atmosphere, followed by filtration of the product under an inert atmosphere.
The use of compounds of formula XIV in their solid, isolated, form offers several important advantages over their preparation in situ, for example measurement of quantities is much easier so the use of an excess is not necessary, and so after the reaction the need to remove the excess is reduced or eliminated, there are fewer side-reactions, and the reaction generally is much cleaner.
It will be apparent to those skilled in the art that compounds of formula XIV may be used in the preparation of other important prostaglandins outside the scope of the formula shown in Figure II, for example a prostaglandin compound having a 3-oxooct-1-enyl, 3-hydroxyoct-1-enyl, 3-oxooctyl or 3hydroxyoctyl sidechain, more particularly PGE" PGE2, PGF2fZ and ca rbocyclin (i.e. the analogue of PGI2 wherein the 6,9-epoxy group is replaced by a 6,9-methylene group), and this forms a further feature of the present invention.
Yet another important feature of the multistage process for the preparation of compounds of the formula shown in Figure II is the formation of the side-chain in the 6-position, and Reference Examples 35, 39,41, 47, 50 and 53 illustrate the novel use of a reagent in the formation of a side-chain in the 6position.
Thus, according to a further feature of the present invention, there is provided a process for the preparation of a compound of the general formula shown in Figure XVI II (wherein A', A2, R2, R3, R4, R5, R6 and Z1 are as hereinbefore defined) from compounds of the general formula shown in Figure XIX (wherein A2, R4, R5 and Z' are as hereinbefore defined) by reaction with a compound of the general formula: : 0=C(R2)A1CH(R3)COOR6 XX
(wherein A', R2, R3 and R6 are as hereinbefore defined), preferably in an inert organic solvent such as an
ether, e.g. tetrahydrofuran, preferably in dry conditions, preferably under an inert atmosphere, and
preferably at a low temperature, e.g. -780C to -650C, characterised in that the reaction is carried out
in the presence of a compound of the general formula: M2[(R'7)3Si]2N XXI (wherein M2 represents an alkali metal, preferably lithium, atom and R" represents an alkyl group of from 1 to 4 carbon atoms, preferably a methyl group).
The presence of a compound of formula XXI in the reaction mixture helps to minimise side reactions and to ensure that the side-chain becomes attached to the bicyclo[3,3,0]octane skeleton in the desired position.
The following Examples illustrate the preparation of the compounds of the present invention, and the Reference Examples illustrate the preparation of the intermediate compounds of the formula shown in Figure VI. Reference Example 5 illustrates the preparation of the key intermediate compounds of the formula shown in Figure XII. Reference Example 22 illustrates the use of a compound of formula XIV in its solid, isolated, form and Reference Example 55 illustrates its preparation. Reference Examples 35, 39, 41, 47, 50 and 53 illustrate the use of a reagent of formula XXI.
The compounds prepared in the Examples and Reference Examples contain various centres of chirality and the products are mixtures of all possible diasterioisomers unless otherwise specified, and each one is accompanied by an equal quantity of its enantiomer. However, the two hydrogen atoms attached to the bridgehead carbon atoms in the bicyclononane and bicyclooctane rings are always cis with respect to each other.According to the convention customarily employed, and with the structures laid out as in the formula drawings, the said hydrogen atoms attached to the bridgehead carbon atoms are said to be in the p-configuration, and the sidechain attached in the 1 2-position is cis with respect to the said hydrogen atoms when the said sidechain is said to be in the s-configuration. The designation (+) indicates that such compounds are accompanied by equal quantities of their enantiomers.
Example 1
Compound A 6-[(E)-3-tert-Butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl]-3-[(E)-4-methoxycarbonyl- butylidene]-bicyclo[3,3,0]octane-2-one (1 2mg), prepared as described in Reference Example 15 and in
the form of (+)-6,B-[(E)-3(r-tert-butyidimethylsilyloxy-3-cycloheXylprop-1 -enyl]-3-[(E)-4-methoxy carbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 5S)-6a oxo-6,9-methano- 1 5-tert-butyldimethylsilyloxy-l 5-cyclohexyl- 16,17,18,1 9,20-pentanorprosta-5, 13- dienoate, and a solution of water, glacial acetic acid and tetrahydrofuran (0.5ml; 35:65:10 by volume) were stirred together at 400C for 4 hours.After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with diethyl ether. The ethereal extracts were washed with aqueous sodium bicarbonate solution (1 M) and then with saturated aqueous sodium chloride solution, and dried over anhydrous sodium sulphate. Concentration under reduced pressure gave 6 [(E)-3-cyclohexyl-3-hydroxyprop- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2- one ( 1 2.7mg), in the form of (+)-6p-[(E)-3-cyclohexyl-3cr-hydroxyprop-1 -enyl]-3-[(E)-4-methoxy carbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 5S)-6aoxo-6,9-methano- 1 5-cyclohexyl- 1 5-hydroxy-l 6,17,18,1 9,20-pentanorprosta-5, 1 3-dienoate.
Example 2
Compound B
A mixture of 6-[(E)-3-cyclohexyl-3-hydroxyprop-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]- bicyclo[3,3,0]octan-2-one (12.7mg), prepared as described in Example 1 and in the form of (+)-6,B- [(E)-3-cyclohexyl-3 a-hydroxyprop- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2- one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 SS)-6a-oxo-6,9-methano-1 5-cyclohexyl-15hydroxy-16,17,18,1 9,20-pentanorprosta-5,1 3-dienoate, lithium hydroxide (21 .5mg), methanol (2ml), and water (0.7ml) was left to stand at the ambient temperature for 1 hour. The mixture was then diluted with water (5ml) and washed with dichloromethane.The aqueous layer was separated and acidified to pH 3 by treatment with aqueous hydrochloric acid (2N). The acidic solution was extracted with dichloromethane, and this extract was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure, to give 3-[(E)-4 carboxybutylidene]-6-[(E)-3-cyclohexyl-3-hydroxyprop-1 -enyl]bicyclo[3 .3 ,0]octan-2-one (6.6mg), in the form of (t)-3-[(E)-4-ca rboxybutylidene]-6p-[(E)-3-cyclohexyl-3a-hydroxyprop-1 -enyl]bicyclo [3,3,0]octan-2-one, otherwise known as (+)-(5E,1 3E)-(9S,1 SS)-6a-oxo-6,9-methano-1 5-cyclohexyl- 15-hydroxy-l 6,17,18,1 9,20-pentanorprosta-5,1 3-dienoic acid.
Example 3
Compound C
A solution of 3-[(E)-4-carboxybutylidenej-6-[(E)-3-cyclohexyl-3-hydroxyprop- 1 -enyl] bicyclo [3,3,0]octan-2-one (6.6mg), prepared as described in Example 2 and in the form of (+)-3-[(E)-4 carboxybutylidene]-6p-[(E)-3-cyclohexyl-3 a-hydroxyprop- 1 -enyl]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-(5E,1 3E)-(9S,l SS)-6a-oxo-6,9-methano-1 5-cyclohexyl-1 5-hydroxy-1 6,17,1 8,1 9,20- pentanorprosta-5,13-dienoic acid in acetone (1 ml) and benzene (4ml) was irradiated in a quartz vessel with ultra-violet rays of wavelength 254nm, emitted by a low pressure mercury vapour lamp, for 20 hours.The solution was then concentrated under reduced pressure and subjected to preparative thin layer chromatography on silica gel, eluting four times with a mixture of ethyl acetate, hexane and formic acid (40:60:1 by volume).
The more polar component (0.8mg) was unchanged starting material [NMR in deuterochloroform: multiplets at 6.5, 5.4, 3.8, 3.6, l.0--3.0p.p.m.].
The less polar component (1.2mg) was (+)3-j[mixture of (E) and (Z)]-4-carboxybutylidene}-6p- [(E)-3-cyclohexyl-3α-hydroxyprop-1-enyl]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-[mixture of (5E) and (5Z)],(1 3E)-(9S,1 5S)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy-16,17,18,19,20- pentanorprosta-5,1 3-dienoic acid (1.2mg). [NMR in deuterochloroform: multiplets at 6.5, 5.9, 5.8, 5.6, 5.4 and 1.0-3.0p.p.m.
The (Z)-isomer in the said less polar component slowly isomerised back to the (E)-isomer at the ambient temperature.
Example 4
Compound D
6-[(E)-(mixture of 3a and 3ss)-tert-Butyldimethylsilyloxyoct-1-enyl]-3-[(E)-4-methoxycarbonyl- butylidene]bicyclo[3,3,0]octan-2-one (14.1 mg), prepared as described in Reference Example 27 or 28 and predominantly in the 6p-configuration, otherwise known as (+)-methyl (5E,1 3E)-(9S),[mixture of (1 5R) and (15S)]-6a-oxosilyloxyprosta-5,13-dienoate, and a solution of water, glacial acetic acid and tetrahydrofuran (0.5ml; 7:13:2 by volume) were stirred together at 400C for 4 hours. After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with diethyl ether.
The ethereal extracts were washed with aqueous sodium bicarbonate solution (1 M) and then with saturated aqueous sodium chloride solution, and then dried over anhydrous sodium sulphate.
Concentration under reduced pressure gave 6-[(E)-(mixture of 3a and 3P)-hydroxyoct-l -enyl]-3-[(E)-4- methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one (11.1 mg), predominantly in the 6p- configuration, otherwise known as (+)-methyl (5E.13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9- methano-1 5-hydroxyprosta-S,1 3-dienoate. [NMR in deuterochloroform: multiplets at 6.5, 5.5,4.1, 3.0-0.7p.p.m., singlet at 3.7p.p.m.; AmaXEtOH 244 nm,
Example 5
Compound E
A stirred solution of 6-[(E)-(mixture of 3a and 3ss)-hydroxyoct-1-enyl]-3-[(E)-4-methoxy- carbonylbutylidene]bicyclo[3,3,0]octan-2-one (90mg), prepared as described in Example 4 and predominantly in the 6p-configuration, otherwise known as (+)-methyl (5E,1 3E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13-dienoate, in methanol (2ml) at 50C was treated with aqueous lithium hydroxide solution (1.5ml; 1.ON). The resulting solution was stirred for 3.3 hours at 10 C and was then treated with glacial acetic acid (0.1 ml).The methanol was removed in vacuo and the residue was diluted with ethyl acetate (1 Oml), washed with hydrochloric acid (2N), then with water, and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated in vacuo, to give 3-[(E)-4-carboxybutylidene]-6-[(E)-(mixture of 3a and 3P)- hydroxyoct-1-enyl]bicyclo[3,3,0]octan-2-one (70.0mg), in the 6p-configuration, otherwise known as (+)-(5E,1 3E)-(9S),[mixture of (1 5R) and (15S)]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13-dienoic
acid [NMR in deuterochloroform: multiplets at 6.6, 5.5, 4.8-3.7, 3.0-1.1, 1 .1-O.7p.p.m.].
Example 6
Compound F
A solution of 3-[(E)-4-carboxybutylidene]-6-[(E)-(mixture of 3a and 3ss)-hydroxyoct-1- enyl]bicyclo[3,3,0]octan-2-one (1 Omg), prepared as described in Example 5 and predominantly in the 6p-configuration, otherwise known as (+)-(5E,1 3E)-(9S),[mixture of (15R) and (15S)]-6a-oxo-6,9- methano-15-hydroxyprosta-5,13-dienoic acid, in benzene (5ml) and acetone (0.5ml) was irradiated for 1 9 hours with ultra-violet rays of wavelength 254nm, emitted by a low pressure mercury vapour lamp.
The solution was then concentrated in vacuo and the residue was subjected to preparative thin layer chromatography on silica gel, using a mixture of ethyl acetate, hexane and glacial acetic acid (50:50:1 by volume) as eluant, to give 3-[(Z)-4-carboxybutylidene]-6-[(E)-(mixture of 3 and 3,B)-hydroxyoct-1- enyl]bicyclo[3,3,0]octan-2-one (2.7mg), predominantly in the 6p-configuration, otherwise known as (+)-(5Z,13E)-(9S),[mixture of (1 5R) and (15S)]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13-dienoic acid [NMR (in deuterochloroform): multiplets at 6.0, 5.5,4.5-3.8, 3.6-1.1, 1.1-0.7 p.p.m.]; and recovered 3-[(E)-4-ca rboxybutylidene]-6-[(E)-( mixture of 3a and 3p)-hydroxyoct- 1 -enyl]bicyclo- [3,3,0]octan-2-one (3.6mg).
Example 7
Compound G
A solution of 6-[(E)-(mixture of 3a and 3P)-hydroxyoct-l -enyl]-3-[(E)-4-methoxycarbonyl- butylidene]bicyclo[3,3,0]octan-2-one (20mg), prepared as described in Example 4 and predominantly in the 6,B-configuration, otherwise known as ()methyl (5E,1 3E)-(9S),[mixture of (15R) and (1 5S)]-6a- oxo-6,9-methano-1 S-hydroxyprosta-S,1 3-dienoate, in methanol (5ml) was irradiated for 20 hours with ultra-violet rays of wavelength 254nm, emitted by a low pressure mercury vapour lamp.The solution was carefully concentrated in vacuo and then subjected to preparative thin layer chromatography on silica gel, using a mixture of ethyl acetate and hexane (1 :4 v/v) as eluant, to give 6-[(E)-(mixture of 3a and 3ss)-hydroxyoct-1-enyl]-3-[(Z)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one (5.0mg), predominantly in the 6/3-configuration, otherwise known as (+)-methyl (5Z,13E)-(9S),[mixture of (1 SR) and (15S]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13-dienoate [NMR in deuterochloroform: multiplets at 5.9, 5.5,4.1, 3.0-1.1, 1 .1-O.7p.p.m., singlet at 3.7p.p.m.]; and recovered 6-[(E) (mixture of 3 and 3ss)-hydroxyoct-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2- one (3.6mg) [NMR in deuterochloroform: multiplets at 6.5,5.5,4.1,3.0-1.1, 1.1-O.7p.p.m., singlet at 3.7p.p.m.].
Example 8
Compound H
A solution of 6-[(E)-(mixture of 3 and 3,B)-tert-butyidimethylsilyloxy-3-cyclohexylprop-1-enyl]- 3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one (43mg), prepared as described in
Reference Example 34 and predominantly in the 6,3-configurntion, otherwise known as (+)-methyl (5E,1 3E)-(9S),[mixture of (I SR) and (15S)]-6a-oxo-6,9-methano-15-tert-butyldimethylsilyloxy-15- cyclohexyl-l 6,1 7,1 8,1 9,20-pentanorprosta-5, 1 3-dienoate, in a mixture of tetrahydrofuran, glacial acetic acid and water (1 ml; 2:13:7 by volume) was stirred for 60 hours at 200C.The solution was then concentrated in vacuo, to give 6-[(E)-3-cyclohexyl-(mixture of 3a and 3P)-hydroxyprop-l -enyl]3-[(E)- 4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one (33mg), predominantly in the 6p- configuration, otherwise known as (+)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9- methano-1 S-cyclohexyl-1 S-hydroxy-1 6,17,1 8,1 9,20-pentanorprosta-S,1 3-dienoate.
Example 9
Compound I
A stirred solution of 6-[(E)-3-cyclohexyl-(mixture of 3a and 3p)-hydroxyprop-1 -enyl]-3-[(E)-4 methoxycarbonylbutylidene]bicyclol3,3,0]octan-2-one (20mg), prepared as described in Example 8 and predominantly in the 6,3-configuration, otherwise known as (+)-methyl (5E,13E)-(9S),[mixture of (15R) and (1 SS)]-6a-oxo-6,9-methano-1 S-cyclohexyl-1 S-hydroxy-1 6,17,18,1 9,20-pentanorprosta- 5,13-dienoate, in methanol (3.2ml) was treated with lithium hydroxide (33.8mg) and water (1.1 ml) at 200C and stirred for 2 hours. The mixture was then concentrated in vacuo.The resulting residue was diluted with water (5ml) and then acidified to pH 3 by treatment with dilute hydrochloric acid (2N). The aqueous layer was then extracted with ethyl acetate (5x5 ml). The combined organic extracts were dried over anhydrous sodium sulphate and concentrated in vacuo.The resulting residue was subjected to chromatography on a silica gel column, using a mixture of ethyl acetate, hexane and formic acid (50:50:1 by volume) as eluant, to give 3-[(E)-4-carboxybutylidene]-6-[(E)-3-cyclohexyl-(mixture of 3a and 3p)-hydroxyprdp-1 -enyl]bicyclo[3,3,0]octan-2-one (1 Omg), predominantly in the 6p-configuration, otherwise known as (+)-(5E,1 3E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 S- cyclohexyl-15-hydroxy-16,17,18,19,20-pentanorprosta-5,13-dienoic acid [NMR in deuterochloroform: multiplets at 6.5, 5.5, 4.2-3.5, 3.O-O.7p.p.m.].
Example 10
Compound J
A stirred solution of 6-[(E)-3-cyclohexyl-(mixture of 3a and 3p)-hydroxyprop-1 -enyl]-3-[(E)-4
methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one (6.7mg), prepared as described in Example 8
and predominantly in the 6,3-configuration, otherwise known as (+)-methyl (5E,1 3E)-(9S),[mixture of
(15R) and (1 SS)]-6a-oxo-6,9-methano-1 S-cyclohexyl-1 S-hydroxy-1 6,17,18,1 9,20-pentanorprosta- 5,13-dienoate, in tetrahydrofuran (0.5ml) at -780C was treated dropwise with a solution of lithium trisec-butylborohydride in tetrahydrofuran (0.0196ml; 1.OM). The stirred solution was allowed to warm to 0 C during 2 hours and then it was stirred for a further period of 2 hours.Glacial acetic acid (0.15ml) and water (0.2ml) were then added to the mixture, which was then concentrated in vacuo.
The resulting residue was dissolved in ethyl acetate (1 Oml), washed with aqueous sodium bicarbonate solution (2M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated in vacuo. The resulting oil was treated with a solution of sodium hydroxide in 50% v/v aqueous methanol (0.1 ml; 2.5N) and stirred for 1 50 minutes at 2O0C. The mixture was then carefully concentrated in vacuo, and diluted with water (6ml) and diethyl ether (3ml). The aqueous phase was separated, acidified to pH 3 by treatment with hydrochloric acid (2N), and extracted with ethyl acetate (4x 1 Oml). The combined organic extracts were dried over sodium sulphate and concentrated in vacuo. The resulting residue was subjected to chromatography on a silica gel column, using a mixture of ethyl acetate, hexane and glacial acetic acid (50:50:1 by volume) as eluant, to give 3-[(E)-4-carboxybutylidene]-6-[(E)-3-cyclohexyl-( mixture of 3 and 3p)-hydroxyprop- 1 -enyl]-2hydroxybicyclo[3,3,0]octane (3.6mg), predominantly in the 6p,6a-configuration, otherwise known as (t)-(SE,1 3E)-(6aS,9S),-[mixture of (1 SR) and (15S)]-6a,15-dihydroxy-6,9-methano-15-cyclohexyl- 16,17,18,19,20-pentanorprosta-5,13-dienoic acid [NMR in deuterochloroform: multiplets at 5.5,3.5, 2.70.7p.p.m.].
Example 11
Compound K (E)-6-(3-tert-Butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxycarbonylcyclo hexylidene)bicyclo[3,3,0]octan-2-one (15mg), prepared as described in Reference Example 36 and in the form of ()-(E)-6ss-(3α-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxyca rbonyl cyclohexylidene)bicyclo[3,3,0]octan-2-one, otherwise known as (+)-ethyl(l 3E)-(9S,1 5S)-6a-oxo-2,5- ethano-6,9-methano-15-tert-butyldimethylsilyloxy-15-cyclohexyl-16,17,18,19,20-pentanorprosta- 5,13-dienoate and a solution of water, glacial acetic acid and tetrahydrofuran (0.59ml; 7:13:2 by volume) were stirred together at 450C for 5 hours.After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with ethyl acetate. The extract was washed with aqueous sodium carbonate solution (2M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.The residue was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (2:1 v/v) as eluant, to give (E)-6-(3-cyclohexyl-3-hydroxyprop-1-enyl)-3-(4-ethoxy- carbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one (8.6mg), in the form of (+)-(E)-6,B-(3-cyclohexyl- 3a-hydroxyprop- 1 -enyl)-3-(4-ethoxycarbonylcyclohexylidene)bicyclo[3,3,O]octan-2-one, otherwise known as ()-ethyl(13E)-(9S,15S)-6a-oxo-2,5-ethano-6,9-methano-15-hydroxy-1 5-cyclohexyl 16,17,18,1 9,2O-pentanorprosta-S,1 3-dienoate [NMR (in deuterochloroform): multiplets at 5.4 5.6, 3.6-4.3 and 0.8--3.0p.p.m.].
Example 12
Compound L
By proceeding in a manner similar to that described in Example 1, but replacing the starting material by the appropriate quantity of 6-[(E)-3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1 -enyl]-3 [(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one, prepared as described in Reference
Example 38 and in the form of ()-6ss-[(E)-3ss-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl]-3- [(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one, otherwise known as (i)-methyl (5E, 1 3E)-(9S. 1 5R)-6a-oxo-6,9-m etha no-l 5-tert-butyldi m ethylsilyloxy-l 5-cyclohexyl- 16,17,18,1 9,20-pentanorprosta-5,1 3-dienoate, there was prepared 6-[(E)-3-cyclohexyl-3 hydroxyprop-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one, in the form of ( +)-6oB-[(E)-3-cyclohexyl-3,B-hydroxyprop-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]-bicyclo- [3,3,0]octan-2-one, otherwise known as (+)-methyl (5E, 1 3E)-(9S,1 5R)-6a-oxo-6,9-m etha no-l 5- cyclohexyl-l 5-hydroxy-l 6,17,18,1 9,20-pentanorprosta-5, 13-dienoate.
Example 13
Compound M
By proceeding in a manner similar to that described in Example 2, but replacing the starting material by the appropriate quantity of 6-[(E)-3-cyclohexyl-3-hydroxyprop- 1 -enyl]-3-[(E)-4-methoxy carbonylbutylidene]bicyclo[3,3,0]octan-2-one, prepared as described in Example 12 and in the form of (+)-6,B-[(E)-3-cyclohexyl-3p-hydroxyprop-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,Oj- octan-2-one, otherwise known as (+)-methyl (5E,13E)-(9S,15R)-6a-oxo-6,9-methano-15-cyclohexyl- 15-hydroxy-l 6,17,18,1 9,20-pentanorprosta-5,1 3-dienoate, there was prepared 3-[(E)-4-carboxy butylidene]-6-[(E)-3-cyclohexyl-3-hydroxyprop-1 -enyl]bicyclo[3,3,O]octan-2-one, in the form of (+)-3 [(E)-4-carboxybutylidene]-6ss-[(E)-3-cyclohexyl-3ss-hydroxyprop-1 -enyl]bicyclo[3,3,0]octan-2-one, otherwise known as ()-(5E,13E)-(9S,15R)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy- 16,17,18,1 9,20-pentanorprosta-S, 1 3-dienoic acid [NMR (in deuterochloroform): multiplets at 6.3 6.6, 5.3-5.6, 4.3-4.8, 4.0-4.3, 1 .O-3.Op.p.m.].
Example 14
Compound N
By proceeding in a manner to that described in Example 11, but replacing the starting material by the appropriate quantity of 6-[(E)-3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl]-3-{[mixture of (E) and (Z)]-S-ethoxycarbonylpentylid-2-enelbicyclo[3,3,O]octan-2-one, prepared as described in
Reference Example 40 and in the form of (+)-6ss-[(E)-3X3-tert-butyidimethylsilyloxy-3-cycloheXylprop-1- enyl]-3-{[mixture of (E) and (Z)1-5-ethoxyca rbonylpentylid-2-ene)bicyclo[3,3 ,0]octan-2-one, otherwise known as (i)-ethyl [mixture of (5E) and (5Z)],(13E)-(9S,15R)-6a-oxo-6,9-methano-5- methyl-1 S-tert-butyldimethylsilyloxy-1 5-cyclohexyl-1 6,1 7,1 8,1 9,2O-pentanorprosta-S,1 3-dienoate, there was prepared 6-[(E)-3-cyclohexyl-3-hydroxyprop-1 -enyl]-3-([mixture of (E) and (Z)]-5-ethoxy carbonylpentylid-2-eneXbicyclo[3,3,0]octan-2-one, in the form of (+)-GP-[(E)-3-cyclohexyl-3P- hydroxyprop-1 -enyl]-3-{[mixture of (E) and (Z)]-5-ethoxyca rbonylpentyl id-2-ene }bicyclo[3,3,0]octan-2- one, otherwise known as (+)-ethyl [mixture of (5E) and (5Z)],(13E)-(9S,15R)-6a-oxo-6,9-methano-5- methyl-l 5-cyclohexyl-l 5-hydroxy-l 6,1 7,18,1 9,20-pentanorprosta-5, 18-dienoate [NMR (in deutero chloroform): 5.3-5.6, 3.9--4.3, 3.6--3.9. 1.0--2.2p.p.m.].
Example 15
Compound O
By proceeding in a manner similar to that described in Example 11, but replacing the starting material with the appropriate quantity of (E)-6-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1 -enyl)- 3-(4-ethoxywarbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one, prepared as described in Reference
Example 42 and in the form of (+)-(E)-625-(3p-tert-butyidimethylsilyloxy-3-cycloheXylprop-1-enyl)-3- (4ethoxycarbonylcycIohexylidene)bicyclo[3,3,O]octan-2-one, otherwise known as (+)-ethyl (13E)- (9S,15R)-6a-oxo-2,5-ethano-6,9-methano- 1 S-tert-butyldimethylsilyloxy-1 5-cyclohexyl- 16.17,18,1 9,20-pentanorprosta-5,1 3-dienoate, there was prepared (E)-6-(3-cyclohexyl-3hydroxyprop-1 -enyl)-3-(4-ethoxyCarbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one, in the form of (+) (E)-6,3-(3-cyclohexyl-3,3-hydroxyprop-1 -enyl)-3-(4-ethoxywarbonylcyclohexyiideneXbicyclo [3,3,0]octan-2-one, otherwise known as (+)-ethyl (1 3E)-(9S,1 SR)-6a-oxo-2,5-ethano-6,9-methano-1 S- hydroxy-l 5-cyclohexyl-l 6,1 7,18,1 9,20-pentanorprosta-5, 13-dienoate [NMR (in deuterochloroform): multiplets at 5.3--5.6, 3.6--4.5, O.7-3.2p.p.m.].
Example 16
Compound P
6-[(E)-3-tert-Butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]- bicyclo[3,3,0]octan-2-one (21 mg), prepared as described in Reference Example 48 and in the form of ( +)-6p-[(E)-3,B-tert-butyidimethylsilyloxy-4-phenoxybut-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]- bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 SS)-6a-oxo-6,9-methano 1 5-tert-butyldimethyisilyloxy-l 6-phenoxy-l 7,18,1 9,20-tetranorprosta-5,1 3-dienoate, and a solution of water, glacial acetic acid and tetrahydrofuran (0.86ml; 7:13:2 by volume) were stirred together at 4O0C for 4 hours.After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with diethyl ether. The ethereal extract was washed with aqueous sodium bicarbonate solution (1 M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated under reduced pressure.The residue was subjected to medium pressure chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1 :1 v/v), to give 6-[(E)-3-hydroxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo- [3,3,0]octan-2-one (15.9mg), in the form of (+)-6iB-[(E)-3p-hydroxy-4-phenoxybut-1-enyl]-3-[(E)-4- methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,13E)- (9s,1 SS)-6a-oxo-6,9-methano-1 S-hydroxy-1 6-phenoxy-1 7,18,1 9,20-tetranorprosta-S,1 3-dienoate [NMR (in deuterochloroform): multiplets at 6.6-7.2, 6.3-6.6, 5.3-5.6, 4.3-4.5, 3.7-3.9, 1.3- 2.8p.p.m., singlet at 3.6p.p.m.].
Example 17 Compounds 6-[(E)-3-tert-Butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidenej- bicyclo[3,3,0]octan-2-one (1 3mg), prepared as described in Reference Example 51 and in the form of (+)-6iB-[(E)-3-tert-butyidimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonyl butylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,13E)-(9S,1 5R)-6a-oxo-6,9 methano-l 5-tert-butyldimethylsilyloxy-l 6-phenoxy-17,18,1 9,20-tetranorprosta-5,1 3-dienoate, and a solution of water, glacial acetic acid and tetrahydrofuran (0.53ml; 7:13::2 by volume) were stirred together at 400C for 4 hours. After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with diethyl ether. The extract was washed with aqueous sodium bicarbonate solution (1 M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.The residue was subjected to medium pressure liquid chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (9:13 v/v), to give 6-[(E)-3-hydroxy-4-phenoxybut-1-enyl]-3-[(E)-4-methoxywarbonyl- butylidene]bicyclo[3,3,0]octan-2-one (9.9mg), in the form of (+)-6p-[(E)-3-hydroxy-4-phenoxybut-1 enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one, otherwise known as (+)-methyl (5E, 13E)-(9S,1 SR)-6a-oxo-6,9-methano- 1 S-hydroxy-1 6-phenoxy- 17,18,1 9,20-tetranorprosta-S, 13- dienoate [NMR (in deuterochloroform): multiplets at 6.8-7.3, 6.4-6.6, 5.6-5.8, 4.3-4.6, 3.8- 4.0, 1.3--2.8p.p.m., singlet at 3.6p.p.m.].
Example 18
Compound R
6-[(E)-3-tert-B utyldimethylsi lyloxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]- bicyclo[3,3,0]octan-2-one (4.1 mg), prepared as described in Reference Example 54, and in the form of (+)-6p-[(E)-(mixture of 3a and 3ss)-tert-butyldimethylsilyloxy-4-phenoxybut-1-enyl]-3-[(E)-4-methoxy- carbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)methyl (5E,1 3E)-(9S), [mixture of (1 5R) and (1 SS)]-6a-oxo-6,9-methano-1 S-tert-butyldimethylsilyloxy-1 6-phenoxy-1 7,18,19,20- tetranorprosta-5,1 3-dienoate, and a solution of water, glacial acetic acid and tetrahydrofuran (1.0ml; 7:13:2 by volume) were stirred together at 40 C for 4 hours. After cooling, the mixture was diluted with saturated aqueous sodium chloride solution and extracted with diethyl ether. The extract was washed with aqueous sodium bicarbonate solution (1 M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated under reduced pressure.The residue (3.0mg) was subjected to preparative thin layer chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (3:2 v/v), to give 6-[(E)-3-hydroxy-4-phenoxybut-1 -enyl]-3-[(E)-4 methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one (0.9mg), in the form of (l)-6,3-[(E)-(mixture of 3a and 3,3)-hydroxy-4-phenoxybut- 1 -enyl)-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan- 2-one, otherwise known as (+)-methyl (5E,13E)-(9S),[mixture of (15R) and (15S)]-6a-oxo-6,9- methano-1 S-hydroxy-1 6-phenoxy-1 7,1 8,1 9 ,20-tetranorprosta-S, 1 3-dienoate.
Reference Example 1
2-Methoxybutadiene (69.9g), 2-cyclopentenone (68.22g) and hydroquinone (0.2g) were heated together at 1 900C for 2 hours. The product was then distilled in vacuo, to give 3-methoxybicyclo [4,3,0]non-3-en-7-one (30g), b.p. 74--920C/1.SmmHg.
Reference Example 2
Diisobutylaluminium hydride (28.5ml) was added dropwise to a stirred solution of 3-methoxy bicyclo[4,3,0]non-3-en-7-one (20g; prepared as described in Reference Example 1) in diethyl ether (600ml), with cooling to -1 00C. The resulting solution was stirred at OOC for 3 hours and then it was treated with methanol (20my). A gelatinous precipitate was formed, and the mixture was then treated with an hydros sodium sulphate (200g) and filtered with the aid of diatomaceous earth. The filtrate was concentrated at reduced pressure, to give 7-hydroxy-3-methoxybicycío[4,3,0]non-3-ene (16g).
Reference Example 3
Acetic anhydride (62.39) was added to a solution of 7-hydroxy-3-methoxybicyclo[4,3,0] non-3- ene (51.49; prepared as described in Reference Example 2) in pyridine (166ml), and the mixture was left to stand at the ambient temperature for 60 hours. Concentration under reduced pressure gave 7 acetoxy-3-methoxybicyclo[4,3,0]non-3-ene (58.9g).
Reference Example 4
Concentrated hydrochloric acid (3.5ml) was added to a solution of 7-acetoxy-3-methoxybicyclo [4,3,0]non-3-ene (58.9g; prepared as described in Reference Example 3) in methanol (500ml), and the mixture was stirred at the ambient temperature for 5 minutes. It was then concentrated under reduced pressure, to give 7-acetoxybicyclo[4,3,0]nonan-3-one (54.79).
Reference Example 5
Thallic nitrate trihydrate (133g) was added to a stirred solution of 7-acetoxybicyclo[4,3,O]nonan3-one (57g; prepared as described in Reference Example 4) in glacial acetic acid (290ml). After 30 minutes the resulting solid was filtered off and the filtrate was heated at reflux for 30 minutes. The acetic acid was distilled off under reduced pressure and the residue was neutraiised by treatment with aqueous sodium hydroxide solution to pH 5 and then with aqueous sodium bicarbonate solution to neutral. The resulting solution was washed with diethyl ether, and then it was acidified by treatment with concentrated hydrochloric acid and extracted with chloroform.The combined chloroform extracts were washed with saturated aqueous sodium chloride solution, then dried over magnesium sulphate, and concentrated under reduced pressure. The residue was purified by medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate, hexane, and acetic acid (45:55:1 by volume), to give 6-acetoxy-2-carboxybicyclo[3,3,0]octane (7.55g), predominantly in the 2,3- configuration.
Reference Example 6
A mixture of 6-acetoxy-2-carboxybicyclo[3,3,O]octane (2.23g; prepared as described in
Reference Example 5 and predominantly in the 2,3-configuration), methanol (25ml), and concentrated hydrochloric acid (0.15ml), was heated at reflux for 16 hours. The mixture was then concentrated under reduced pressure to a third of its previous volume, and it was then added to an excess of water.
The mixture was extracted with dichloromethane. The combined dichloromethane extracts were washed with saturated aqueous sodium bicarbonate solution, and then with saturated aqueous sodium chloride solution, and then it was dried over anhydrous sodium sulphate. Concentration under reduced pressure gave crude 6-hydroxy-2-methoxycarbonylbicyclo[3,3,O]octane (1 .98g), predominantly in the 2,3-configuration, and pure enough for use in the next stage.
Reference Example 7
A solution of crude 6-hydroxy-2-methoxy-carbonylbicyclo[3,3,O]octane (1.98g; prepared as described in Reference Example 6 and predominantly in the 2,3-configuration) in dichloromethane (1 Oml) was added to a stirred suspension of pyridinium chlorochromate (3.5g) in dichloromethane (35ml), and the mixture was stirred for 2 hours. The mixture was then diluted with diethyl ether (200ml), and the supernatant liquid was decanted onto a short column of silica gel. The residue was triturated with diethyl ether several times until it become granular in aspect and then all the ethereal washings were added to the column.The column was eluted with diethyl ether and the eluate was evaporated, to give crude 2-methoxy-carbonybicyclo[3,3,0]octan-6-one (1.71 g) [infra-red (I.R.) 1740cm-11, predominantly in the 2,3-configuration, and pure enough for use in the next stage.
Reference Example 8
A mixture of crude 2-methoxycarbonylbicyclo[3,3,O]octan-6-one (1.35; prepared as described in
Reference Example 7 and predominantly in the 2,3-configuration), ethylene glycol (1.84g), benzene (1 00ml), and a trace of p-toluenesulphonic acid was heated at reflux for 1 6 hours, collecting the water that formed in a Dean and Stark apparatus. After cooling, the mixture was diluted with diethyl ether, and washed successively with aqueous sodium carbonate solution (1 M) and then with saturated aqueous sodium chloride solution, and then it was dried over anhydrous sodium sulphate.
Concentration under reduced pressure gave crude spiro{6-methoxywarbonylbicyclo[3,3,0]octane-2,2'- dioxolanel (1.56g) [I.R. 940, 1730cm-'], predominantly in the 6,3-configuration, and pure enough to use in the next stage.
Reference Example 9
A solution of spiroj6-methoxycarbonylbicyclo[3,3,O]octane-2,2'-dioxolane{ (0.9g; prepared as described in Reference Example 8 and predominantly in the 6,3-configuration) in anhydrous tetrahydrofuran (4ml) was added slowly to a stirred solution of lithium tri-sec-butylborohydride in tetrahydrofuran (12.2ml; 1 M) at -780C in an atmosphere of argon. The solution was stirred at -780C for 1 hour and was then stirred at the ambient temperature for 2 hours.The mixture was then treated with aqueous sodium hydroxide solution (9.3ml; 3M), keeping the temperature at OOC. The mixture was then treated slowly with aqueous hydrogen peroxide solution (6.3ml; 30% w/v), keeping the temperature at OOC, and then the mixture was stirred at OOC for a further period of 30 minutes. The mixture was then diluted with water and treated with sufficient sodium chloride to form a saturated solution and then it was extracted with diethyl ether. The combined extracts were washed with saturated aqueous sodium chloride solution and dried over anhydrous sodium sulphate and then concentrated under reduced pressure.The residue (0.779) was subjected to medium pressure, short column chromatography on silica gel using a mixture of ethyl acetate and methanol (40:1 v/v) as eluant, to give spirof6-hydroxymethylbicyclo[3,3,0]octane-2,2'-dioXolanel (0.459), I.R.3400cm-', predominantly in the 6,3-configuration.
Reference Example 10
A solution of spiro(6-hydroxymethylbicyclo[3,3,O]octane-2,2'-dioxolanei (0.459; prepared as described in Referehce Example 9 and predominantly in the 6,3-configuration) in dichloromethane (2.1 ml) was added to a stirred suspension of pyridinium chlorochromate (0.749) in dichloromethane (7.5ml). The mixture was stirred at the ambient temperature for 3 hours, and was then treated with an excess of diethyl ether. A gum separated, and the supernatant liquid was added carefully to the top of a short silica gel chromatography column. The gum was triturated several times with diethyl ether until it become granular in aspect. The ethereal solutions and the granules were also added to the top of the column.The column was then eluted with diethyl ether, and the combined eluant was concentrated in vacuo, to give spiro{6-formylbicyclo[3,3,0]octane-2,2'-dioxolane} (0.379), predominantly in the 6j- configuration.
Reference Example 11
Dimethyl (2-cyclohexyl-2-oxoethyl)phosphonate (0.53g) was added to a stirred suspension of sodium hydride dispersion (106mg; 50% w/w in mineral oil) in dry tetrahydrofuran (16ml) in an argon atmosphere and the mixture was stirred for 1 6 hours at room temperature. Spiro{6-formylbicyclo [3,3,0]octane-2,2'-dioxolane) (0.379; prepared as described in Reference Example 10 and predominantly in the 6,3-configuration) was then added, and the mixture was stirred for a further period of 2 hours. The mixture was then concentrated under reduced pressure, and the residue was dissolved in diethyl ether. The ethereal solution was washed with water, dried over sodium sulphate and concentrated under reduced pressure.The residue was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (1:3 v/v) as eluant, to give (E)-spirn{ 6-(3-cyclohexyl-3-oxoprop-1 -enyl)bicyclo[3,3,0]octane-2,2'-dioxolanel (0.289), predominantly in the 6,3-configuration.
Reference Example 12
A solution of (E)-spiro( 6-(3-cyclohexyl-3-oxoprop-1 -enyl)bicyclo[3,3,0]octane-2,2"-dioxolan (0.289; prepared as described in Reference Example 11 and predominantly in the 6,3-configuration) in dry tetrahydrofuran (1.Oml) was added to a stirred solution of lithium tri-sec-butylborohydride in tetrahydrofuran (1.88ml; 1 M) at -780C under an atmosphere of argon. The mixture was stirred at -780C for 1 hour and then at ambient temperature for 2 hours.It was then treated with aqueous sodium hydroxide solution (1.34ml; 3M) at OOC, followed by aqueous hydrogen peroxide solution (0.88ml; 30% w/v), and the mixture was stirred for a further period of 30 minutes at OOC. The mixture was treated with water and sufficient sodium chloride to form a saturated solution, and then it was extracted with diethyl ether. The extract was washed with saturated aqueous sodium chloride solution and then dried over anhydrous sodium sulphate, and concentrated under reduced pressure, to give (E) spiro( 6-(3-cyclohexyl-3-hydroxyprop-l -enyl)bicyclo[3.3,0]octane-2,2'-dioxolan (0.28g) predominantly in the 6p-configuration and pure enough for use in the next stage.
Reference Example 13
A mixture of (E)-spi ro { 6-(3-cyclohexyl-3-hydroxyprop- 1 -enyl)bicyclo[3,3,0]octane-2,2'- dioxolane) (0.28g; prepared as described in Reference Example 1 2 and predominantly in the 6p- configuration) and 60% w/v aqueous acetic acid was left to stand at ambient temperature for 1 6 hours.
An excess of water was then added and the mixture was extracted with diethyl ether. The combined ethereal extracts were washed with aqueous sodium carbonate solution (1 M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated under reduced pressure, to give (E)-2-(3-cyclohexyl-3-hydroxyprop- 1 -enyl)bicyclo[3,3,0]octan-6-one (0.239), predominantly in the 2,3-configuration. This material was purified and separated into 2 epimeric pairs of enantiomers by short column chromatography on silica gel eluting with a mixture of ethyl acetate and hexane (1:3 v/v).The less polar component (89mg) was (+)-(E)-2p-(3-cyclohexyl-3p-hydroxyprop- 1 -enyl)bicyclo[3,3,0]octan-6-one and the more polar component (41 mg) was cyclohexyl-3a-hydroxyprop-1 -enyl)bicyclo[3,3,0]octan-6-one.
Reference Example 14
A mixture of (E)-2-(3-cyclohexyl-3-hydroxyprop-1 -enyl)bicyclo[3,3,O]octan-6-one (41 mg), prepared as described in Reference Example 13, and in the form of (+)-(E)-2,3-(3-cycIohexyl-3a- hydroxyprop-1 -enyl)bicyclo[3,3,O]octan-6-one, tert-butyldimethylchlorosilane (35mug), and imidazole (29.5mg) in dimethylformamide (0.25ml) was stirred at ambient temperature for 3 hours. Water (2.5ml) was added and the mixture was extracted with diethyl ether.The combined ethereal extracts were washed with saturated aqueous sodium chloride solution, then dried over anhydrous sodium sulphate, and concentrated under reduced pressure to give (E)-2-(3-tert-butyldimethylsilyloxy-3-cyclo- hexylprop-1 -enyl)bicyclo[3,3,O]octan-6-one (57mg), in the form of (+)-(E)-2ss-(3-tert-butyidimethyl- silyloxy-3-cyclohexylprop-1 -enyl)bicyclo[3 ,3,0]octan-6-one.
Reference Example 15 (E)-2-(3-tert-Butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)bicyclo[3,3,0]octan-6-one (57mug), prepared as described in Reference Example 14 and in the form of (+)-(E)-2,3A3a-tert-butyldimethyl- silyloxy-3-cyclohexylprop- 1 -enyl)bicyclo[3,3,0]octan-6-one, was dissolved in dry diethyl ether (0.6ml). The solution was cooled to -700C under an atmosphere of argon and treated with a solution of lithium diisopropylamide in diethyl ether (0.132ml;; 1 .3M) with stirring, and the mixture was stirred at -700C for 10 minutes. 4-Methoxycarbonylbutanal (29.6mg) was then added to the mixture, which was stirred for a further period of 10 minutes at -700C. The mixture was then treated with a solution of glacial acetic acid (0.05ml) in diethyl ether (1 ml) and the mixture was allowed to warm to ambient temperature. Water and diethyl ether were added and the layers were separated. The aqueous layer was extracted with ethyl acetate. The combined diethyl ether and ethyl acetate solutions were washed with saturated aqueous sodium bicarbonate solution and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.The resulting residue was dissolved in dry benzene (0.6ml). The solution was treated with methanesulphonyl chloride (0.015ml) with stirring at the ambient temperature, and then with triethylamine (0.0255ml), and was stirred for a further period of 2 hours. The mixture was then treated with 1,8diazabicyclo[5,4,0]undec-7-ene (137mg) and stirred for 16 hours at ambient temperature.The crude mixture was then subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1:9 by volume), to give (E)-6-(3-tert-butyidimethyisilyloxy- 3-cyclohexylprop-1 -enyl)-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one (12mug), in the form of (i)-6,3-[(E)-3-tert-butyldi methylsilyloxy-3-cyclohexylprop- 1 -enyl]-3-[(E)-4-methoxy carbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (i)-methyl (5E,1 3E)-(9S,1 5S)-6a oxo-6,9-methano- 1 S-tert-butyldimethylsilyloxy-1 5-cyclohexyl- 1 6,1 7,1 8,1 9,20-pentanorprosta-S, 1 3- dienoate.
Reference Example 16
The sodium salt of dimethyl (2-oxoheptyl)phosphonate (325mg) was added to a stirred solution of spiro{6-formylbicyclo[3,3,0]octane-2,2'-dioxolane| (125 mg; prepared as described in Reference
Example 10 and predominantly in the 6,3-configuration) and the suspension was stirred at the ambient temperature under an argon atmosphere for 16 hours. Glacial acetic acid (6 drops) was then added, and the mixture was concentrated under reduced pressure. The residue was dissolved in water, and extracted with diethyl ether.The extract was dried over anhydrous sodium sulphate and concentrated under reduced pressure, and the residue was subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1:2 v/v to give (E)spiro{6-(3-oxooct-1 -enyl)bicyclo[3,3,0]octane-2,2"-dioxolan (128mg), predominantly in the 6ss- configuration.
Reference Example 17
A solution of (E)-spiro( 6-(3-oxooct-1 -enyl)bicyclo[3,3,0]octane-2,2'-dioxolanej (128mg; prepared as described in Reference Example 1 6 and predominantly in the 6,3-configuration) in dry tetrahydrofuran (0.5ml) was added to a stirred solution of lithium tri-sec-butylborohydride in tetrahydrofuran (0.88mi; 1 M) at -780C under an argon atmosphere. The mixture was stirred at -780C for a further period of one hour and then at the ambient temperature for a period of 2 hours.The mixture was then cooled to OOC and treated with aqueous sodium hydroxide solution (0.67my; 3M), followed by aqueous hydrogen peroxide solution (0.44ml; 30% w/v) and the mixture was stirred for a further period of 30 minutes at OOC. The mixture was treated with water and sufficient sodium chloride to form a saturated solution, and then it was extracted with diethvl ether. The extracts were washed with saturated aqueous sodium chloride solution and dned over anhydrous sodium sulphate, and concentrated under reduced pressure.The resulting residue was subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (3:2 v/v), to give (E)-spiro( 6-[( mixture of 3a and 3,3)-hydroxyoct-1 -enyl]bicyclo[3,3,O]octane-2,2'-dioxolane) (85mug), predominantly in the 6ss-configuration.
Reference Example 18
A mixture of (E)-spiroI 6-[(mixture of 3 a' and 3p)-hydroxyoct-1 -enyl]bicyclo[3,3,0]octane-2,2'- dioxolanej (85mg; prepared as described in Reference Example 17 and predominantly in the 6ss- configuration) and aqueous acetic acid solution (2.5ml; 60% v/v) was left to stand at the ambient temperature for 1 6 hours. An excess of water was then added and the mixture was extracted with diethyl ether.The extract was washed with aqueous sodium carbonate solution (1 M) and then with saturated aqueous sodium chloride solution, then it was dried over an hydros sodium sulphate and concentrated under reduced pressure, to give (E)-2-[(mixture of 3a' and 3,3)-hydroxyoct-1 -enyl]- bicyclo[3,3,0]octan-6-one (67mg), predominantly in the 2,3-configuration.
Reference Example 19
A mixture of (E)-2-[(mixture of 3cr and 3ss)-hydroxyoct-1-enyl]-bicyclo[3,3,0]octan-6-one (67 mg; prepared as described in Reference Example 18 and predominantly in the 2,3-configuration), tert-butyldimethylchlorosilane (60mg), and imidazole (50mg) in dimethylformamide (0.4ml) was stirred at the ambient temperature for 3 hours. Water (5ml) was then added and the mixture was extracted with diethyl ether.The extract was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure, to give (E)-2-[(mixture of 3a and 3P)-tert-butyldimethylsilyloxyoct-l -enyl]bicyclo[3,3,0]octan-6-one (87 mug), predominantly in the 2,3-configuration.
Reference Example 20
A solution of 6-acetoxy-2-carboxybicyclo[3,3,0]octane (3.29; prepared as described in Reference
Example 5 and predominantly in the 2,3-configuration) in tetrahydrofuran (30ml) at -400C was treated with a solution of diborane in tetrahydrofuran (15ml; 1 M) under an argon atmosphere. The solution was stirred for 1 hour at -400C and then it was allowed to warm to OOC during 1 hour and was maintained at OOC for a further period of 150 minutes. The solution was then allowed to warm to 200C during 17 hours. The solution was then treated with aqueous ammonium chloride solution (10% w/v) and the mixture was concentrated in vacuo.The resulting residue was treated with ethyl acetate (100ml) and washed with water (50ml) and then with saturated aqueous sodium bicarbonate solution, and then was dried over anhydrous sodium sulphate. The solution was concentrated in vacuo to give an oil, which was subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and toluene (1 :1 v/v), to give 6-acetoxy-2-hydroxymethylbicyclo [3,3,0]octane (2.89), predominantly in the 2,3-configuration.
Reference Example 21
A stirred solution of 6-acetoxy-2-hydroxymethylbicyclo[3,3,0]octane (0.10g; prepared as described in Reference Example 20 and predominantly in the 2,3-configuration) in dry dichloromethane (2.5ml) was treated with pyridinium chlorochromate (0.229) and anhydrous sodium bicarbonate (0.0839). The resulting suspension was stirred for 1 50 minutes at the ambient temperature and was then placed directly onto a short column of silica gel and subjected to medium pressure, short column chromatography, eluting with a mixture of ethyl acetate and toluene (1:1 v/v) to give 6-acetoxy-2-formylbicyclo[3,3,0]octane (0.070g), predominantly in the 2,3-configuration.
Reference Example 22
A suspension of the sodium salt of dimethyl (2-oxoheptyl)phosphonate (0.105g; prepared as described in Reference Example 55) in tetrahydrofuran (5.3ml) at OOC was treated dropwise with a
solution of 6-acetoxy-2-formylbicyclo[3,3,O]octane (70mg; prepared as described in Reference
Example 21 and predominantly in the 2,3-configuration) in tetrahydrofuran (1.owl) with stirring. The
resulting mixture was stirred for 18 hours at ambient temperature under an atmosphere of argon. The
mixture was then treated dropwise with a solution of glacial acetic acid (200mg) in diethyl ether (1 ml),
and then the solution was concentrated in vacuo.The residue was treated with diethyl ether (20ml) and water (20ml) and the organic layer was separated. The aqueous layer was extracted with diethyl
ether (3 x20ml). The combined extracts were washed with water and then with saturated aqueous
sodium chloride solution, dried over magnesium sulphate and concentrated in vacuo, to give an oil
which was subjected to chromatography on a silica gel column, to give (E)-6-acetoxy-2-(3-oxooct-1
enyl)bicyclo[3,3,0]octane (65.7mg), predominantly in the 2,3-configuration.
Reference Example 23 (E)-6-Acetoxy-2-(3-oxooct- 1 -enyl)bicyclo[3 ,3,0]octane (400mg; prepared as described in
Reference Example 22 and predominantly in the 2,3-configuration) was dissolved in an hydros tetrahydrofuran (1 Oml) at -780C in an argon atmosphere with stirring and then it was treated with
hexamethylphosphotriamide (400mg) and then, dropwise, with a solution of potassium tri-sec
butylborohydride in tetrahydrofuran (1.5ml; 1.OM). The solution was then allowed to warm to --300C during 3 hours and then the mixture was treated with a mixture of ethyl acetate (20ml), water (50ml)
and hydrochloric acid (1 ml; 2N).The organic layer was removed and the aqueous layer was extracted
with ethyl acetate (3x50ml). The combined organic layers were washed with saturated aqueous
sodium bicarbonate solution and then with saturated aqueous sodium chloride solution, dried over
an hydros sodium sulphate, and concentrated in vacuo. The resulting oil was subjected to medium
pressure, short column chromatography on silica gel, eluting with mixtures of ethyl acetate and hexane
of varying proportions (beginning at 1:9 v/v and ending at 1:1 v/v), to give (E)-6-acetoxy-2-[(mixture of 3a and 3,3)-hydroxyoct-1 -enyl]bicyclo[3,3,0]octane (390mg), predominantly in the 2P-configuration.
Reference Example 24
(E)-6-Acetoxy-2-[(mixture of 3a and 3p)-hydroxyoct-1 -enyl]bicyclo[3,3,0]octane (380mug; prepared as described in Reference Example 23 and predominantly in the 2,3-configuration) was dissolved in dry dimethylformamide (8mi), with stirring, at 200 C. The solution was then treated with imidazole (330mug) and tert-butyldimethylchlorosilane (420mug) and stirred for 15 hours at 200C.The
reaction mixture was then treated with ethyl acetate (50ml) and saturated aqueous sodium bicarbonate solution (50ml). The organic layer was separated, washed with water (30ml) and then with saturated aqueous sodium chloride solution, then dried over snhydrous sodium sulphate.
Concentration in vacuo at 14mmHg and then at O.OSmmHg agave (E)-6-acetoxy-2-[(mixture of 3a and 3p)-tert-butyidimethylsilyloxyoct-1-enyl]bicyclo[3,3,0]octane (530mg) in the form of an oil, which slowly solidified. It was predominantly in the 2,3-configuration.
Reference Example 25
A solution of (E)-6-acetoxy-2-[(mixture of 3a and 3p)-tert-butyidimethylsilyloxyoct-1 -enyl]bicyclo[3,3,0]octane (530mg; prepared as described in Reference Example 24 and predominantly in the 2,3-configuration) in methanol (1 Oml) was treated with an hydros potassium carbonate (193mug).
The mixture was heated at 400 C, with stirring, for 24 hours. The methanol was then removed in vacuo and the residue was extracted with dichloromethane (3x 1 Oml). Concentration of the extract in vacuo gave (E)-2-[(mixture of 3 and 3p)-tert-butyidimethylsilyloxyoct-1 -enyl]-6-hydroxybicyclo [3,3,0]octane (430mg), predominantly in the 2,3-configuration.
Reference Example 26
A stirred solution of (E)-2-[(mixture of 3α and 3ss)-tert-butyldimethylsilyloxyoct-1-enyl]-6- hydroxybicyclo[3,3,O]octane (430mg prepared as described in Reference Example 25 and predominantly in the 2,3-configuration) in dry dichloromethane (15ml) was treated with potassium acetate (127mg), potassium carbonate (110mug) and pyridinium chlorochromate (561mg), and the mixture was stirred for 1 50 minutes at 200C. A further quantity of pyridinium chlorochromate (120mg) was then added and the mixture was stirred for a further period of 1 hour.The mixture was then concentrated in vacuo and subjected to chromatography on a silica gel column, using a mixture of ethyl acetate and hexane (1:4 v/v) as eluant, to give (E)-2-[(mixture of 3 < x and 3,3)-tert-butyldimethyl- silyloxyoct-l -enyl]bicyclo[3,3,0]octan-6-one (330mg), predominantly in the 2,3-configuration.
Reference Example 27
(E)-2-[(mixture of 3 and 3P)-tert-Butyl-dimethylsi lyloxyoct-l -enyl]bicyclo[3,3 ,O]octa n-6-one (290mg), prepared as described in Reference Example 19 or 26 and predominantly in the 2,3- configuration, was dissolved, with stirring, in anhydrous diethyl ether (5ml) at -780C in an argon atmosphere, and the solution was then treated with a freshly prepared solution of lithium diisopropylamide in diethyl ether (1.1 ml; O.9M) at -780C and the mixture was stirred at -780C for 10 minutes.
The mixture was then treated with 4-methoxycarbonylbutanal (208mg) at -780C and stirred for a further period of 10 minutes. The reaction mixture was then treated with a solution of glacial acetic acid (100mg) in diethyl ether (0.1 ml) at -780C, and was then warmed to 200 C. The mixture was then diluted with ethyl acetate (25ml), washed with aqueous ammonium chloride solution (2M) and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated in vacuo.The resulting oil (480mg) was dissolved with stirring in dry benzene (semi) and then was treated with methanesuiphonyl chloride (124mug) and then, dropwise, with a solution of triethylamine (101 mg) in benzene (0.5ml) at 200C.The mixture was stirred for 1 hour at 200C and then was treated with 1 ,8-diazabicyclo[5,4,O]undec-7-ene (1.owl) and the resulting mixture was stirred for a further period of 1.5 hours at 200 C. The mixture was concentrated in vacuo to give a residue which was subjected to chromatography on a silica gel column using a mixture of hexane and ethyl acetate (1::9 v/v) as eluant, to give 6-[(E)-(mixture of 3a and 3p)-tert-butyidimethylsilyloxyoct-1- enyl]-3-[(E)-4-methoxycarbonylbutylidene]-bicyclo[3,3,O]octan-2-one (92.4mg), predominantly in the 6,3-configuration, otherwise known as (+)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo- 6,9-methano- 15-tert-butyidimethylsilyloxy-prosta-5,13-dienoate.
Reference Example 28 (E)-2-[( mixture of 3 cur and 3P)-tert-Butyldimethylsi lyloxyoct-l -enyl]bicyclo[3,3 ,Olocta n-6-one (87mg; prepared as described in Reference Example 19 or 26 and predominantly in the 2,3- configuration), pyrrolidine (34mg), cyclohexane (1 ml), and anhydrous magnesium sulphate (20mg) were stirred together and heated at reflux under an argon atmosphere for 8 hours. After cooling, the mixture was filtered, and the magnesium sulphate residue was washed with a further quantity of cyclohexane. The combined filtrate and washing were concentrated under reduced pressure.The residue was stirred with 4-methoxycarbonylbutanal (38mg) and anhydrous magnesium sulphate (20mg) in toluene and in an argon atmosphere at 950C for 5 hours. The mixture was then cooled and filtered, and the filtrate was concentrated under reduced pressure. The resulting residue was stirred with a mixture of tetrahydrofuran and water (2ml; 1:1 by volume) for 16 hours at room temperature.
The mixture was then extracted with diethyl ether, and the combined ethereal extracts were washed with saturated aqueous sodium chloride solution, dried over an hydros sodium sulphate, and concentrated under reduced pressure. The resulting residue was subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1::4 v/v), to give 6-[(E)-(mixture of 3a and 3,3)-tert-butyldi methylsilyloxyoct- 1 -enyl]-3-[(E)-4-methoxycarbonyl- butylidene]bicyclo[3,3,0]octan-2-one (14.1 mg), predominantly in the 6,3-configuration, otherwise known as (*)-methyl (5E,13E)-(9S),[mixture of (15R) and (1 SS)]-6a-oxo-6,9-methano-1 5-tert-butyl- dimethylsilyloxyprosta-5,13-dienoate [NMR in deuterochloroform: multiplets at 6.5, 5.4, 4.1 and 1.03.0p.p.m., singlets at 3.7, 0.8 and O.OSp.p.m.].
Reference Example 29
Dimethyl (2-cyclohexyl-2-oxoethyl)-phosphonate (3.3g) was added to a stirred suspension of sodium hydride (0.349) and 1,4,7,10,13,1 6-hexaoxacyclooctadecane (1 Omg) in dry tetrahydrofuran (9Oml) in an atmosphere of argon and the mixture was stirred for 12 hours. The mixture was then cooled to 0 C and treated dropwise with 6-acetoxy-2-formylbicyclo[3,3,0]octane (1.05g; prepared as described in Reference Example 21 and predominantly in the 2p-configuration). The reaction mixture was allowed to warm to room temperature and then it was stirred for a further period of 24 hours. A solution of glacial acetic acid (400mg) in diethyl ether (1 ml) was then added and the mixture was concentrated in vacuo.The residue was dissolved in ethyl acetate (1 Oml) and the resulting solution was washed with water (1 Oml) and then with saturated aqueous sodium chloride solution (1 Oml), dried over magnesium sulphate, and concentrated in vacuo. The resulting oil was treated with acetic anhydride (1 ml) and pyridine (4ml) at 200C and left to stand for 12 hours. Concentration at 0.01 mmHg gave an oil, which was subjected to chromatography on a silica gel column, eluting with mixtures of ethyl acetate and hexane (1:6 v/v and then 1:1 v/v), to give (E)-6-acetoxy-2-(3-cyclohexyl-3-oxoprop 1-enyl)bicyclo[3,3,0]octane (0.849), predominantly in the 2*configuration.
Reference Example 30
A stirred solution of (E)-6-acetoxy-2-[(mixture of 3a and 3,3)-cyclohexyl-3-oxoprop-1 -enyl] bicyclo[3,3,0]octane (0.84g; prepared as described in Reference Example 29 and predominantly in the 2,3-configuration) in dry tetrahydrofuran (11.oil) and hexamethylphosphotriamide (2ml) in an atmosphere of argon at -780C was treated, dropwise with stirring, with a solution of potassium trisec-butylborohydride in tetrahydrofuran (3.1 ml; 1.OM). The reaction mixture was then allowed to warm to OOC during 2 hours. The mixture was then treated with acetone (1 ml) and then with glacial acetic acid (1 ml) and stirred at 20 C for 30 minutes. The mixture was then concentrated in vacuo. The residue was subjected to chromatography on a silica gel column, using a mixture of ethyl acetate and hexane (1:6 v/v) as eluant, to give (E)-6-acetoxy-2-[(mixture of 3cz and 3,3)-cyclohexyl-3-hydroxyprop- 1 -enyl]bicyclo[3,3,O]octane (0.32 g), predominantly in the 2,3-configuration.
Reference Example 31
A stirred solution of (E)-6-acetoxy-2-[(mixture of 3a and 3)-cyclohexyI-3-hydrnxyprnp-1 -enyl]bicyclo[3,3,0]octane (0.329; prepared as described in Reference Example 30 and predominantly in the 2,3-configuration) and imidazole (0.259), in dry dimethylformamide (1 Oml) was treated with tert-butyldimethylchlorosilane (0.329) and left to stand at 200C for 60 hours. It was then diluted with water (50ml) and extracted with ethyl acetate (4x20ml).The combined organic extracts were washed with water (1 Oml) and with saturated aqueous sodium chloride solution, dried over magnesium sulphate, and concentrated in vacuo at 0.01 mmHg to give (E)-6-acetoxy-2-[(mixture of 3 a and 3p)-tert-butyl- dimethylsilyloxy-3-cyclohexylprop-l -enyl]bicyclo[3,3,0]octane (0.409), predominantly in the 2,3- configuration.
Reference Example 32
A mixture of (E)-6-acetoxy-2-[(mixture of 3a and 3ss)-tert-butyldimethylsilyloxy-3-cyclo- hexylprop-1-enyl]bicyclo[3,3,O]octane (0.49; prepared as described in Example 31 and predominantly in the 2,3-configuration), potassium carbonate (0.15g) and methanol (1 Oml) was heated at 500C for 8 hours. The mixture was then cooled and concentrated in vacuo. The resulting residue was extracted with dichloromethane (3 x5ml).Concentration in vacuo of the combined extracts gave (E)-2-[(mixture of 3a and 3,3)-tert-butyldimethylsilyl6xy-3-cyclohexylprop- 1 -enyl]-6-hydroxybicyclo[3,3,O]octane, predominantly in the 2,3-configuration, in the form of an oil (0.369), sufficiently pure enough in the next stage.
Reference Example 33
A stirred solution of crude (E)-2-[(mixture of 3cr and 3,3)-tert-butyldimethylsilyloxy-3-cyclo- hexylprop-l -enyl]-6-hydroxybicyclo[3,3,0]octane (0.369; prepared as described in Reference Example 32 and predominantly in the 2,3-configuration) in dichloromethane (15ml) was treated with pyridinium chlorochromate (0.42g) at 200C under an argon atmosphere and stirred for a further period of 3.5 hours. The mixture was then treated with silica gel (29) and carefully concentrated in vacuo.The resulting powder was placed onto a column of silica gel and eluted with a mixture of ethyl acetate and hexane (1 :4 v/v), to give (E)-2-[(mixture of 3a and 3p)-tert-butyidimethylsilyloxy-3-cyclohexylprop-1- enyl]bicyclo[3,3,0]octan-6-one (0.229), predominantly in the 2,3-configuration.
Reference Example 34
A stirred solution of (E)-2-[(mixture of 3a and 3,3)-tert-butyldimethylsilyloxy-3-cyclohexylprop-1- enyl]bicyclo[3,3,0]octan-6-one (0.22g; prepared as described in Reference Example 33 and predominantly in the 2,3-configuration) in dry diethyl ether (3ml) at -780C in an atmosphere of argon was treated with a solution of lithium diisopropylamide in diethyl ether (0.5mi; 1.38M). The solution was stirred for 10 minutes at -780C and then treated with 4-methoxycarbonylbutanal (112mg) at -780C.After stirring for 10 minutes at -780C the reaction mixture was treated with a solution of glacial acetic acid (200mg) in diethyl ether (400mg) and the resulting white suspension was allowed to warm to room temperature. The mixture was then diluted with diethyl ether (20ml) and poured into water (10ml). The organic phase was separated and washed with aqueous sodium bicarbonate solution (5ml; 10% w/v) and then with saturated aqueous sodium chloride solution, dried over magnesium sulphate and concentrated in vacuo. The resulting oil (0.49) was dissolved in benzene (0.2ml) at 2O0C and treated with methanesulphonyl chloride (83mg).The stirred mixture was then treated dropwise with triethylamine (0.095ml) and stirred for a further period of 40 minutes in an argon atmosphere. The mixture was then treated with 1,8-diazabicyclo[5,4,0]undec-7-ene (0.519) and it was stirred for a further period of 2 hours. The mixture was then treated with diethyl ether (20ml) glacial acetic acid (2ml) and water (20ml). The organic phase was separated and the aqueous layer was extracted with diethyl ether (2x 1 Oml). The combined organic solutions were washed with saturated aqueous sodium bicarbonate solution and then dried over anhydrous sodium sulphate.
Concentration in vacuo, followed by chromatography on a silica gel column, using a mixture of ethyl acetate and hexane (1:9 v/v) as eluant, gave 6-[(E)-(mixture of 3a and 3p)-tert-butyidimethylsilyloxy-3- cyclohexylprop- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one (54g), predominantly in the 6,3-configuration, otherwise known as (*)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-methano-15-tert-butyldimethylsilyloxy-15-cyclohexyl-1 6,17,18,19,20pentanorprosta-5,13-dienoate.
Reference Example 35
(E)-2-(3-tert-Butyldimethylsilyloxy-3-cyclohexylprop-1-enyl)bicyclo[3,3,0]octan-6-one 65mg; prepared as described in Reference Example 14, and in the form of ()-(E)-2ss-(3α-tert-butyldimethyl- silyloxy-3-cyclohexylprop-1 -enyl)bicyclo[3,3,0]octan-6-one was dissolved in dry tetra hydrofuran (2.63ml). The solution was cooled to -70"C under an atmosphere of argon and treated with a solution of lithium bis(trimethylsilyl)amide in tetrahydrofuran (1 M; 0.212ml), and the mixture was stirred at --700C for 10 minutes. The mixture was then treated with ethyl 4-oxocyclohexylcarboxylate (59mg) and stirred for a further period of 3 hours at -70 C.The mixture was then treated with a solution of glacial acetic acid (0.02ml) in diethyl ether (0.175ml) and allowed to warm to ambient temperature.
The mixture was then treated with water (20ml) and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium bicarbonate solution, and then with saturated aqueous sodium chloride solution, dried over an hydros sodium sulphate, and concentrated under reduced pressure.
The residual oil was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (1:3 v/v) as eluant, to give (E)-6-(3-tert-butyldimethylsilyloxy-3cyclohexyiprop- 1 -enyl)-3-(4-ethoxyca rbonyl-1 -hydroxycyclohex- 1 -yl)bicyclo[3,3,0]octan-2-one (33mg), in the form of ()-(E)-6ss-(3α-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl)-3-(4-ethoxy- carbonyl-1 -hydroxycyclohex- 1 -yl)bicyclo[3,3,0]octan-2-one.
Reference Example 36 (E)-6-(3-tert-Butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxycarbonyl- 1 -hydroxy cyclohex-1-yl)bicyclo[3,3,0]octan-2-one {32mg; prepared as described in Reference Example 35 and in the form of (+)-(E)-6fi-(3cg-tert-butyidimethylsilyloxy-3-cycloheXylprop-1-enyl)-3-(4-ethoxy- carbonyl-1-hydroxycyclohex-1-yl)bicyclo[3,3,0]octan-2-one} was dissolved in benzene (0.234ml) and treated with methanesulphonyl chloride (0.006ml) with stirring at the ambient temperature and was then treated with triethylamine (0.01 Oml) and stirred for 2 hours.The mixture was then treated with
1,8-diazabicyclo[5,4,0]undec-7-ene (0.055ml) and the mixture was stirred for a further period of 1 50 minutes. The mixture was then treated with water(l5ml) and extracted with ethyl acetate. The ethyl acetate extract was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.The residue was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (1:8 v/v) as el uant, to give (E)-6-(3-tert-butyldimethylsilyloxy-3-cyclohexylprnp- 1 -enyl)-3-(4-ethoxy carbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one (15mg), in the form of (+)-(E)-6p-(3cr-tert-butyl- dimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxycarbonylcyclohexylidene)bicyclo[3,3,0]octan-2- one, otherwise known as (+)-ethyl (1 3E)-(9S"1 SS)-6a-oxo-2,S-ethano-6,9-methano- 1 5-tert-butyl- dimethylsilyloxy-1 5-cyclohexyl- 6,17,18,1 9,20-pentanorprosta-5, 13-dienoate.
Reference Example 37
By proceeding in a manner similar to that described in Reference Example 14, but replacing the starting material by the appropriate quantity of (E)-2-(3-cyclohexyl-3-hydroxyprop-1 -enyl)bicyclo- [3,3,0]octan-6-one, the less polar isomer prepared as described in Reference Example 13, in the form of ()-(E)-2ss-(3-cyclohexyl-3ss-hydroxyprop-1-enyl)bicyclo[3,3,0]octan-6-one, there was prepared (E)2-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl)bicyclo[3,3,0]octan-6-one, in the form of (+) (E)-2,3-(3,3-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)bicyclo[3,3,0]octan-6-one.
Reference Example 38
By proceeding in a manner similar to that described in Reference Example 1 5, but replacing the starting material by the appropriate quantity of (E)-2-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 - enyl)bicyclo[3,3,0]octan-6-one, prepared as described in Reference Example 37 and in the form of (+) (E)-2ss-(3ss-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl)bicyclo[3,3,0]octan-6-one, there was prepared 6-[(E)-3-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl]-3-[(E)-4-methoxycarbonyl butylidene]bicyclo[3,3,0]octan-2-one, in the form of (+)-6,B-[(E)-3jB-tert-butyidimethylsilyloxy-3-cyclo- hexylprop- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]octan-2-one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 SR)-6a-oxo-6,9-methano- 1 5-tert-butyldimethylsilyloxy-1 5-cyclohexyl
16,17,18,19,20-pentanorprosta-5,13-dienoate.
Reference Example 39
(E)-2-(3-tert-Butyldimethylsilyloxy-3-cyclohexylprop-1-enyl)bicyclo[3,3,0]octan-6-one, (65mg), prepared as described in Reference Example 37 and in the form of (+)-(E)-2p-(3p-tert-butyidimethyl- silyioxy-3-cyclohexylprop-1 -enyl)bicyclo[3,3,0]octan-6-one was dissolved in dry tetrahydrofuran (2.63ml). The solution was cooled to -700C under an atmosphere of argon and treated with a solution of lithium bis(trimethylsilyl)amide in tetrahydrofuran (0.212ml; 1 M), and the mixture was stirred at -700C for 10 minutes.The mixture was then treated with ethyl 5-oxohexanoate (55mg) and stirred for a further period of 5 hours at 700 C. The solution was treated with a solution of glacial acetic acid (0.02ml) in diethyl ether (0.175ml) and the mixture was allowed to warm to the ambient temperature.
The mixture was then treated with water (20ml) and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium bicarbonate solution and then with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.
The residual oil was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (1 :6 v/v) as eluant, to give 6-[(E)-3-tert-butyldimethylsilyloxy-3 cyclohexylprop-1 -enyl]-3-(S-ethoxycarbonyl-2-hydrnxypent-2yl)bicyclo[3,3,O]octan2one (22mg), in the form of 6,3-[(E)-3,3-tert-butyldimethylsilyloxy-3-cyclohexyiprop- 1 -enyl]-3-( S-ethoxycarbonyl-2- hydroxypent-2-yl)bicyclo[3,3,0]octan-2-one, otherwise known as (+)-ethyl (13E)-(9S,15R)-6a-oxo- 6,9-methano-15-tert-butyidimethylsiiyloxy-1 5-cyclohexyl-5-hydroxy-5-methyi-16,17,18,19,20- pentanorprosta-1 3-dienoate.
Reference Example 40
A stirred solution of 6-[(E)-3-tert-butyidimethylsilyloxy-3-cycloheXylprop-1-enyl]-3-(5-ethOxy- carbonyl-2-hydroxypent-2-yl)bicyclo[3,3,O]octan-2-one (26mg), prepared as described in Reference
Example 39 and in the form of 6p-[(E)-3,B-tert-butyidimethylsilyloxy-3-cycloheXylprop-1-enyl]-3-(5- ethoxycarbonyl-2-hydroxypent-2-yl)bicyclo[3,3,O]octan-2-one, otherwise known as (+)-ethyl (13E)- (9S. 1 5R)-6a-oxo-6.9-m eth a no- 1 S-tert-butyldimethylsilyloxy-1 S-cyclohexyl-S-hydroxy-S-methyl- 16,17,18,1 9,20-pentanorprost-1 3-enoate, in benzene (0.1 9ml) was treated with methanesulphonyl chloride (0.0048ml) at the ambient temperature, followed by triethylamine (0.008ml) and the mixture was stirred for 2 hours. It was then treated with 1 ,8-diazabicyclo[5,4,O]undec-7-ene (0.0446my) and the mixture was stirred for 2 hours. It was then treated with water (1 Oml) and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium chloride solution, dried over an hydros sodium sulphate, and concentrated under reduced pressure.The resulting residue was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (1 :8 v/v) as eluant, to give 6-[(E)-3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1 enyl]-3-{[mixture of (E) and (Z)]-5-ethoxyearbonylpentylid-2-eneXbicyclo[3,3,0]octan-2-one (22mg), in the form of ()-6ss-[(E)-3ss-tert-butyldimethylsilyloxy-3-cyclohexylprop-1-enyl]-3-{[mixture of (E) and (Z)]-5-ethoxycarbonylpentylid-2-eneibicyclo[3,3,O]octan-2-one, otherwise known as (+)-ethyl [mixture of (5E and (5Z)1,(13E)-(9S,1 SR)-Ga-oxo-6,9-methano-5-methyl-l 5-tert-butyldimethyl-silyloxy-l 5- cyclohexyl-l 6,17,18,1 9,20-pentanorprosta-5, 13-dienoate.
Reference Example 41
By proceeding in a manner similar to that described in Reference Example 35, but replacing the starting material by the appropriate quantity of (E)-2-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1 enyl)bicyclo[3,3,0]octan-6-one, prepared as described in Reference Example 37 and in the form of (+) (E)-2,3-(3,3-tert-butyldimethylsilyloxy-3-cyclohexylprop-1 -enyl)bicyclo[3,3,0]octan-6-one, there was prepared (E)-6-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxycarbonyl- 1 -hydroxy cyclohexyl)bicyclo[3,3,0]octan-2-one, in the form of (+)-(E)-6ss-(3/3-tert-butyidimethylsilyloxy-3-cyclo- hexylprop- 1 -enyl)-3-(4-ethoxycarbonyl-1 -hydroxycyclohex-1 -yl)bicyclo[3,3,0]octan-2-one.
Reference Example 42
By proceeding in manner similar to that described in Reference Example 36, but replacing the starting material by the appropriate quantity of (E)-6-(3-tert-butfidimethylsilyloxy-3-cycioheXylprop-1- enyl)-3-(4-ethoxycarbonyl-l -hydroxycyclohex-l -yl)bicyclo[3,3,0]octan-2-one, prepared as described in
Reference Example 41 and in the form of (+)-(E)-6,3-(3,3-tert-butyldimethylsilyloxy-3-cyclohexylprnp-1 enyl)-3-(4-ethoxycarbonyl- l-hydroxycyclohex-l -yl)bicyclo[3,3,0]octan-2-one, there was prepared (E)6-(3-tert-butyldimethylsilyloxy-3-cyclohexylprop- 1 -enyl)-3-(4-ethoxyca rbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one, in the form of (+)-(E)-6S(3p-tert-butyidimethylsilyloxy-3-cycloheXylprop-1- enyl)-3-(4-ethoxycarbonylcyclohexylidene)bicyclo[3,3,0]octan-2-one, otherwise known as (+)-ethyl (13E)-(9S,15R)-6a-oxo-2,5-ethano-6,9-methano- 5-tert-butyldimethylsilyloxy- 15-cyclohexyl16,17,18,19,20-pentanorprosta-5,13-dienoate.
Reference Example 43
A mixture of the lithium salt of dimethyl (2-oxo-3-phenoxypropyl)phosphonate (0.259), spiro{6 formylbicyclo[3,3,O]octane-2,2'-dioxolane) (0.1 5g), prepared as described in Reference Example 10 and predominantly in the 6,3-configuration, and tetrahydrofuran (9ml) was stirred for 16 hours at room temperature. The mixture was then concentrated under reduced pressure, and then treated with water and diethyl ether. The layers were separated, and the aqueous layer was extracted with a further quantity of diethyl ether. The combined extracts were washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure.The resulting residue was subjected to medium pressure, short column chromatography on silica gel, using a mixture of ethyl acetate and hexane (2:3 v/v) as eluant, to give (E)-spiro( 6-(3-oxo-4-phenoxybut-l - enyl)bicyclo[3,3,0]octan-2,2'-dioxolanej (0.1 6g), predominantly in the 6,3-configuration.
Reference Example 44
A stirred solution of lithium tri-sec-butylborohydride in tetrahydrofuran (1.24ml; 1 M), was treated with a solution of (E)-spiro{ 6-(3-oxo-4-phenoxybut-1 -enyl)bicyclo[3,3,O]octan-2,2'-dioxolane I (0.20g; prepared as described in Reference Example 43 and predominantly in the 6,3-configuration) in drytetrahydrofuran (0.7ml) at -780C under an atmosphere of argon. The mixture was stirred at -'780C for one hour and then at the ambient temperature for 2 hours.It was then treated with aqueous sodium hydroxide solution (1.24ml; 3M) at OOC, followed by aqueous hydrogen peroxide solution (0.62ml; 30% w/v) and the mixture was stirred for a further period of 30 minutes at OOC. The mixture was treated with water and sufficient sodium chloride to form a saturated solution, and then it was extracted with diethyl ether. The extract was washed with saturated sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure, to give (E)-spirol6-(3- hydroxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,0]octan-2 ,2"-dioxola ne ) (0.229), predominantly in the 6p- configuration, and sufficiently pure for use in the next stage.
Reference Example 45
A mixture of (E)-spirot 6-(3-hydroxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,0]octan-2,2'-dioxolane (0.229; prepared as described in Reference Example 44 and predominantly in the 6,3-configuration) and aqueous acetic acid (6ml; 60% w/v) was left to stand at the ambient temperature for 16 hours. The mixture was then treated with an excess of water and extracted with diethyl ether.The extract was washed with aqueous sodium carbonate solution (1 M), and then with saturated aqueous sodium chloride solution, dried over an hydros sodium sulphate, and concentrated under reduced pressure, to give (E)-2-(3-hydroxy-4-phenoxybut-1 -enyl)bicyclo[3,3,0]octan-6-one (0.1 6g), predominantly in the 2,3-configuration. This material was purified and separated into two epimeric pairs of enantiomers by short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1:2 v/v).
The less polar component (49mg) was (+)-(E)-2,3-(3,3-hydrnxy-4-phenoxybut- 1 -enyl)bicyclo [3,3,0]- octan-6-one and the more polar component (27mg) was (+)-(E)-2P-(3L-hydroxy-4-phenoxybut- 1- enyl)bicycip[3,3,0]octan-6-one. A mixture of these components (43mg), that is to say (+)-(E)-2/3- [(mixture of 3 a' and 3jB)-hydroxy-4-phenoxybut-1-enyl]bicyclo[3,3,0]octan-6-one, was also obtained from the column.
Reference Example 46 A mixture of (E)-2-(3-hydroxy-4-phenoxybut-l -enyl)bicyclo[3,3,0]octan-6-one (49mg), the less polar component prepared as described in Reference Example 45 and in the form of (+)-(E)-2,B-(3,8- hydroxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,0]octan-6-one, tert-butyldimethylchlorosilane73 1 mg), and imidazole (29mg) in dimethylformamide (0.11 6ml) was stirred at the ambient temperature for 2 hours.
The mixture was then treated with water (1 Oml) and extracted with diethyl ether. The extract was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate and concentrated under reduced pressure, to give (E)-2-(3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 - enyl)bicyclo[3,3,0]octan-6-one (76mg), in the form of (+)-(E)-2p-(3,B-tert-butyidimethylsilyloxy-4- phenoxybut- 1 -enyl)bicyclo[3,3,O]octan-6-one.
Reference Example 47
A stirred solution of (E)-2-(3-tert-butyidimethylsilyloxy-4-phenoxybut-1-enyl)bicyclo[3,3,0]- octan-6-one (75mg), prepared as described in Reference Example 46 and in the form of (+)-(E)-2p-(3ss- tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,O]octan-6-one, in dry tetrahydrofuran (2.55ml) was cooled to -700C under an atmosphere of argon and treated with a solution of lithium bis(trimethylsilyl)amide in tetrahydrofuran (1 M; 0.204ml), and the mixture was stirred at -700C for 10 minutes.The mixture was then treated with 4-methoxycarbonylbutanal (44mg) and stirred for a further period of 1 50 minutes at 700 C. The mixture was then treated with a solution of glacial acetic acid (0.019ml) in diethyl ether (0.17ml) and allowed to warm to the ambient temperature. The mixture was treated with water (25ml) and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium bicarbonate solution, and with saturated aqueous sodium chloride solution, dried over anhydrous sodium.sulphate, and concentrated under reduced pressure.The residual oil was subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (1:2 v/v), to give 6-[(E)-3-tert-butyidimethylsilyloxy-4-phenoxybut-1-enyl]-3-(1-hydroxy-4- methoxycarbonylbut-1 -yl)bicyclo[3,3,0]octan-2-one (47mg), in the form of (i)-6,3-[(E)-3,3-tert-butyl- dimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4-methoxycarbonylbut-1 -yl)bicyclo[3,3,0]octan2-one.
Reference Example 48
A stirred solution of 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4 methoxycarbonylbut-1-yl)bicyclo[3,3,0]octan-2-one (38mg), prepared as described in Reference
Example 47 and in the form of (+)-6p-[(E)-3A-tert-butyidimethylsilyioxy-4-phenoxybut-1-enyl]-3-(1- hydroxy-4-methoxycarbonylbut- 1 -yl)bicyclo[3,3,0]octan-2-one, in dry benzene (0.28ml) was treated with methanesulphonyl chloride (0.0072ml) at the ambient temperature and then with triethylamine (0.012ml) and stirred for 2 hours. It was then treated with 1 ,8-diazabicyclo[5,4,O]undec-7-ene (65mg) and stirred for 150 minutes.The mixture was treated with water (15ml) and extracted with ethyl acetate. The extract was washed with saturated aqueous sodium chloride solution, dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The resulting residue was then subjected to medium pressure, short column chromatography on silica gel, eluting with a mixture of ethyl acetate and hexane (23: :77 v/v), to give 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]- 3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one (24mg), in the form of (1)-6,3-[(E)-3 - tert-butyldimethylsilyloxy-4-phenoxybut- -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,0]- octan-2-one, otherwise known as (+)-methyl (5E,13E)-19S,15S)-6a-oxo-6,9-methano-15-tert-butyl- dimethylsilyloxy-l 6-phenoxy-l 7,18,1 9,20-tetranorprosta-5, 13-dienoate.
Reference Example 49
By proceeding in a manner similar to that described in Reference Example 46, but replacing the starting material by the appropriate quantity of (E)-2-(3-hydroxy-4-phenoxybut-1 -enyl)bicyclo[3,3,O]- octan-6-one, the more polar component prepared as described in Reference Example 45, and in the form of (+)-(E)-2P-(3a-hydroxy-4-phenoxybut-l -enyl)bicyclo[3,3,0]octan-6-one, there was prepared (E)-2-(3-tert-butyldimethylsilyloxy-4-phenoxybut 1 -enyl)bicyclo[3,3,O]octan-6-one, in the form of (+) (E)-2,3-(3a'-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,0]octan-6-one.
Reference Example 50
By proceeding in a manner similar to that described in Reference Example 47, but replacing the starting material by the appropriate quantity of (E)-2-(3-tert-butyldimethylsilyloxy-4-phenoxybut-1
enyl)bicyclo[3,3,0]octan-6-one, prepared as described in Reference Example 49, and in the form of (+) (E)-2p-(3-tert-butyidimethylsilyloxy-4-phenoxybut-1-enyl)bicyclo[3,3,0]octan-2-one, there was prepared 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4-methoxy carbonylbut-1 -yl)bicyclo[3,3,0]octan-2-one, in the form of ( +)-6,B-[(E)-3a-tert-butyidi methylsilyloxy-4- phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4-methoxycarbonylbut- 1 -yl)bicyclo[3,3,0]octan-2-one.
Reference Example 51
By proceeding in a manner similar to that described in Reference Example 48, but replacing the starting material by the appropriate quantity of 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut-1 enyl]-3-(1-hydroxy-4-methoxycarbonylbut-1-yl)bicyclo[3,3,0]octan-2-one, prepared as described in
Reference Example 50, and in the form of (+)-6,B-[(E)-3c-tert-butyidimethylsilyloxy-4-phenoxybut-1 enyl]3-(l -hydroxy-4-methoxycarbonylbut-l -yl)bicyclo[3,3,0]-octan-2-one, there was prepared 6-[(E) 3-tert-butyldimethylsilyloxy-4-phenoxybut-1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo- [3,3,0]octan-2-one, in the form of (+)-6p-[(E)-3ct-tert-butyidimethylsilyloxy-4-phenoxybut-1-enyl]-3- [(E)-4-methoxycarbonylbutylidene]bicyclo[3,3,O]octan-2-one, otherwise known as (+)-methyl (5E,1 3E)-(9S,1 5R)-6a-oxo-6,9-methano-15-tert-butyldimethylsilyloxy-16-phenoxy-17,18,19,20- tetranorprosta-5,13-dienoate.
Reference Example 52
By proceeding in a manner similar to that described in Reference Example 46, but replacing the starting material by the appropriate quantity of (E)-2-(3-hydroxy-4-phenoxybut-1 -enyl)bicyclo[3,3,O]- octan-6-one, the mixture of components prepared as described in Reference Example 45 and in the form of (+)-(E)-2p-[(mixture of 3a and 3,3)-hydroxy-4-phenoxybut-1 -enyl]bicyclo[3,3,O]octan-6-one, there was prepared (E)-2-(3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl)bicyclo[3,3,0]octan-6one, in the form of (+)-(E)-2,3-[(mixture of 3a and 3,3)-tert-butyldimethylsilyloxy-4-phenoxybut-1 -enyl]- bicyclo[3,3,0]octan-6-one.
Reference Example 53
By proceeding in a manner to that described in Reference Example 47, but replacing the starting material by the appropriate quantity of (E)-2-(3-tert-butyldimethylsilyloxy-4-phenoxybut-1 -enyl)bicyclo[3,3,0]octan-6-one, prepared as described in Reference Example 52, and in the form of (+)-(E) 2p-[(mixture of 3a and 3ss)-tert-butyldimethylsilyloxy-4-phenoxybut-1-enyl]bicyclo[3,3,0]octan-6-one, there was prepared 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4 methoxycarbonylbut-1-yl)bicyclo[3,3,0]octan-2-one, in the form of (+)-6ss-[(E)-(mixture of 3a and 3p)- tert-butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-( 1 -hydroxy-4-methoxycarbonylbut- 1 -yl )bicyclo [3,3,0]octan-2-one.
Reference Example 54
By proceeding in a manner similar to that described in Reference Example 48, but replacing the starting material by the appropriate quantity of 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut-1 enyl]3-(l -hydroxy-4-methoxycarbonylbut-l -yl)bicyclo[3,3,0]octan-2-one, prepared as described in
Reference Example 53, and in the form of (l)-6,3-[(E)-(mixture of 3a and 3p)-tert-butyidimethyl- silyloxy-4-phenoxybut- 1 -enyl] -3-( 1 -hydroxy-4-methoxycarbonylbut- 1 -yI)bicyclo[3,3,O]octan-2-one, there was prepared 6-[(E)-3-tert-butyldimethylsilyloxy-4-phenoxybut-1-enyl]-3-[(E)-4-methoxy- carbonylbutylidene]bicyclo[3,3,0]octan-2-one, in the form of (+)-6,B-[(E)-(mixture of 3a and 3,3)-tert- butyldimethylsilyloxy-4-phenoxybut- 1 -enyl]-3-[(E)-4-methoxycarbonylbutylidene]bicyclo[3.3,0]octan-2- one, otherwise known as (+)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-methano- 15-tert-butyldi methylsiiyloxy-l 6-phenoxy-l 7,18,1 9,20-tetranorprosta-5 , 13-dienoate.
Reference Example 55
A suspensipn of sodium hydride in mineral oil (50% w/w; 96mg) was mixed with tetrahydrofuran (15ml) and treated with dimethyl (2-oxoheptyl)phosphonate (O.48g), dropwise, at OOC under an atmosphere of argon. After 90 minutes the precipitated solid was filtered off under an atmosphere of nitrogen, washed with tetrahydrofuran (2x5ml) and dried in vacuo at 200C/O.01 mmHg, to give the sodium salt of dimethyl (2-oxoheptyl)phosphonate (180mg), m.p. 130-1320C [Elemental analysis:
C,44.2; H,7.4; P,12.4%; calculated: C,44.2; H,7.4; P,12.7%].
The present invention includes within its scope pharmaceutical compositions which comprise at least one compound of the general formula shown in Figure II or, when R' represents a hydrogen atom, a non-toxic salt thereof, or a cyclodextrin clathrate thereof, together with a pharmaceutical carrier or coating. In clinical practice the compounds of the formula shown in Figure II will normally be administered orally, rectally, vaginally or parenterally.
Methods of presentation of pharmaceutically active compounds are well known in the art and a suitable vehicle may be determined by the physician, pharmacist or veterinarian, depending upon such factors as the effect sought, the size, age, sex and condition of the patient and, for veterinary uses, the species of the animal to be treated, and on the physical properties of the active compound. The compositions may also contain, as is usual in the art, such materials as solid or liquid diluents, wetting agents, preservatives, flavouring and colouring agents and the like.
Solid compositions for oral administration include compressed tablets, pills, dispersible powders, and granules. In such solid compositions one or more of the active compounds is, or are, admixed with at least one inert diluent such as calcium carbonate, potato starch, alginic acid, or lactose. The compositions may also comprise, as is normal practice, additional substances other than inert diluents, e.g. lubricating agents, such as magnesium stearate. Liquid compositions for oral administration include pharmaceutically-acceptable emulsions, solutions, suspensions, syrups and elixirs containing inert diluents commonly used in the art, such as water and liquid paraffin. Besides inert diluents such compositions may also comprise adjuvants, such as wetting and suspending agents, and sweetening, flavouring, perfuming and preserving agents.The compositions according to the invention, for oral administration, also include capsules of absorbable material such as gelatin containing one or more of the active substances with or without the addition of diluents or excipients.
Solid compositions for vaginal administration include pessaries formulated in manner known per se and containing one or more of the active compounds.
Solid compositions for rectal administration include suppositories formulated in manner known perse and containing one or more of the active compounds.
Preparations according to the invention for parenteral administration include sterile aqueous or non-aqueous solutions, suspensions, or emulsions. Examples of non-aqueous solvents or suspending media are propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable organic esters such as ethyl oleate. These compositions may also include adjuvants such as preserving, wetting, emulsifying and dispersing agents. They may be sterilised, for example, by filtration through a bacteria-retaining filter, by incorporation of sterilising agents in the compositions, by irradiation, or by heating. They may also be manufactured in the form of sterile solid compositions, which can be dissolved in sterile water or some other sterile injectable medium immediately before use.
The compounds of the formula shown in Figure II may alternatively be administered orally by any method known per se for administration by inhalation of drugs which are not themselves gaseous under normal conditions of administration. Thus, a solution of the active ingredient in a suitable pharmaceutically-acceptable solvent, for example water, can be nebulized by a mechanical nebulizer, for example a Wright Nebulizer, to give an aerosol of finely-divided liquid particles suitable for inhalation. The active ingredients may also be administered orally by inhalation in the form of aerosols generated from self-propelling pharmaceutical compositions.
The percentage of active ingredient in the compositions of the invention may be varied, it being necessary that it should constitute a proportion such that a suitable dosage for the therapeutic effect desired shall be obtained. Obviously several unit dosage forms may be administered at about the same time. In general, the preparations should normally contain at least 0.025% by weight of active substance when required for administration by injection; for oral administration the preparations will normally contain at least 0.1% by weight of active substance. The dose employed depends upon the desired therapeutic effect, the route of administration and the duration of the treatment. In the adult, the doses are generally between 0.0002 and 2.0mg/kg body weight. If necessary these doses may be repeated as and when required.
Claims (46)
1. A prostaglandin analogue of the general formula:
(wherein R' represents a hydrogen atom or a straight- or branched-chain alkyl group, Y1 represents a carbonyl or hydroxymethylene group, - - - - - represents a single or double bond, Al represents an alkylene linkage containing 2 or 3 carbon atoms and optionally bearing a methyl or ethyl substituent,
R2 represents a hydrogen atom or a methyl or ethyl group and R3 represents a hydrogen atom ol else R2 and R3 form an alkylene linkage containing 2 or 3 carbon atoms, optionally bearing a methyl or ethyl substituent, such that the symbols A', R2 and R3, together with the carbon atoms through which they are connected, form a cycloalkyl ring of 6, 7 or 8 carbon atoms, optionally bearing one or two methyl or ethyl substituents, X1 represents an ethylene or trans-vinylene group, Y2 represents a carbonyl or hydroxymethylene group, and either (i) A2 represents a straight- or branched-alkylene chain containing from 1 to 3 carbon atoms, Z' represents a direct bond or an oxygen or sulphur atom, and R4 represents a straight- or branched-chain alkyl group containing from 1 to 1 2 carbon atoms, optionally substituted by a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a phenyl group optionally substituted by a halogen atom or by a trifiuoromethyl group or by a straight- or branched-chain alkyl or alkoxy group containing from 1 to 6 carbon atoms, or (ii) A2 and Z' both represent direct bonds and R4 represents a straight- or branchedchain alkyl group containing from 1 to 12 carbon atoms, optionally substituted by a cycloalkyl group containing from 3 to 8 carbon atoms, or represents a cycloalkyl group containing from 3 to 8 carbon atoms), and cyclodextrin clathrates thereof and, when R' represents a hydrogen atom, non-toxic salts thereof.
2. A prostaglandin analogue according to claim 1 wherein R' represents a hydrogen atom or a straight- or branched-chain alkyl group containing from 1 to 6 carbon atoms.
3. A prostaglandin analogue according to claim 1 or 2 wherein the hydrogen atoms attached to the 8- and 9-positions are both in beta configuration.
4. A prostaglandin analogue according to claim 1,2 or 3 wherein the sidechain attached to the
12-position is cis with respect to the hydrogen atoms attached to the 8- and 9-positions.
5. A prostaglandin analogue according to claim 1 conforming to the general formula:
(wherein A', A2, R', R2, R3, R4, - - - - -, X1, y1, Y2 and Z1 are as hereinbefore defined) and their
enantiomers, and non-toxic salts and cyclodextrin clathrates thereof.
6. A prostaglandin analogue according to any one of the preceding claims wherein Al represents an ethylene group.
7. A prostaglandin analogue according to any one of the preceding claims wherein A2 represents a direct bond or a methylene group.
8. A prostaglandin analogue according to any one of the preceding claims wherein R4 represents a straight- or branched-chain alkyl group containing from 1 to 12 carbon atoms or represents a cycioalkyl group containing from 3 to 8 carbon atoms or a phenyl group.
9. A prostaglandin analogue according to claim 8 wherein the straight- or branched-chain alkyl group contains from 4 to 8 carbon atoms.
10. A prostaglandin analogue according to claim 8 wherein the straight- or branched-chain alkyl group is pentyl and the cycloalkyl group is cyclohexyl.
11. A prostaglandin analogue according to any one of the preceding claims wherein R' represents a hydrogen atom or a methyl or ethyl group.
1 2. A prostaglandin analogue according to any one of the preceding claims wherein R2 represents a hydrogen atom or a methyl group.
1 3. A prostaglandin analogue according to any one of the preceding claims wherein R3 represents a hydrogen atom.
14. A prostaglandin analogue according to any one of the preceding claims wherein A1, R2 and R3, together with the carbon atoms through which they are connected. form a cvclohexyl ring.
15. A prostaglandin analogue according to any one of the preceding claims wherein represents a vinylene group.
1 6. A prostaglandin analogue according to any one of the preceding claims wherein X' represents a trans-vinylene group.
17. A prostaglandin analogue according to any one of the preceding claims wherein Y2 represents a hydroxymethylene group.
18. A prostaglandin analogue according to any one of the preceding claims wherein Z' represents a direct bond or an oxygen atom.
19. (+)-Methyl (5E,13E)-(9S,15S)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy- 16,17,18,1 9,20-pentanorprosta-5,1 3-dienoate, (+)-(5E,1 3E)-(9S,1 5S)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy-16,17,18,19,20- pentanorprosta-S,1 3-dienoic acid,
(+)-[mixture of (5E) and (5Z)],(1 3E)-(9S,1 SS)-6a-oxo-6,9-methano- 1 5-cyclohexyl- 1 5-hydroxy- 16,1 7,18,1 9,20-pentanorprosta-5,1 3-dienoic acid, (1)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano- 1 5-hydroxyprosta- 5,13-dienoate, (+)-(5E,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13- dienoic acid, (+)-(5Z,13E)-(9S),[mixture of (1 SR) and (15S)]-6a-oxo-6,9-methano-15-hydroxyprosta-5,13- dienoic acid,
(+)-methyl (5Z,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 5-hydroxyprosta- 5,13-dienoate, (1)-methyl (5E,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 5-cyclohexyl-1 S- hydroxy-l 6,1 7,18,1 9,20-pentanorprosta-5, 13-dienoate, (1)-(SE,1 3E)-(9S),[mixture of (1 SR) and (1 SS)j-6a-oxo-6,9-methano-1 5-cyclohexyl-1 5-hydroxy- 16,17,18,19,20-pentanorprosta-5,13-dienoic acid, and
(+)-(5E,1 3E)-(6aS,9S),[mixture of (1 SR) and (15S)]-6a,15-dihydroxy-6,9-methano-15- cyclohexyl-16,17,18,19,20-pentanorprosta-5,13-dienoic acid.
20. (+)-Ethyl (13E)-(9S,15S)-6a-oxo-2,5-ethano-6,9-methano-15-hydroxy-15-cyclohexyl- 16,17,18,1 9,20-pentanorprosta-5, 13-dienoate, (+)-methyl (5E,1 3E)-(9S,1 5R)-6a-oxo-6,9-methano-15-cyclohexyl-15-hydroxy-16,17,18,19,20- pentanorprosta-5,1 3-dienoate, (1)-(SE,1 3E)-(9S,1 SR)-6a-oxo-6,9-methano-1 S-cyclohexyl-1 S-hydroxy-1 6,17,18,1 9,20- pentanorprosta-5,1'3-dienoic acid, (+)-ethyl[mixture of (5E) and (5Z)],(13E)-(9S,15R)-6a-oxo-6,9-methano-5-methyl-15- cyclohexyl-15-hydroxy-16,17,18,19,20-pentanorprosta-5,13-dienoate, (1)-ethyl (1 3E)-(9S,1 5R)-6a-oxo-2,5-ethano-6,9-methano-15-hydroxy-15-cyclohexyl- 16,17,18,1 9,20-pentanorprosta-5, 13-dienoate, (+)-methyl (SE, 1 3E)-(9S, 1 5S)-6a-oxo-6,9-methano-15-hydroxy-16-phenoxy-17,18,19,20- tetranorprosta-5,13-dienoate, (+)-methyl (5E,1 3E)-(9S,1 5R)-6a-oxo-6,9-methano-15-hydroxy-16-phenoxy-17,18,19,20- tetranorprosta-5, 1 3-dienoate and
()-methyl (5E,13E)-(9S),[mixture of (1 SR) and (1 SS)]-6a-oxo-6,9-methano-1 S-hydroxy-1 6- phenoxy-l 7,1 8,1 9,20-tetranorprosta-5,1 3-dienoate.
21. A process for the preparation of prostaglandin analogues of general formula II shown in claim 1, wherein Y2 represents a hydroxymethylene group and the other symbols are as defined in claim 1, which comprises the acid hydrolysis of a compound of the general formula:
wherein A', A2, R', R2, R3, R4, - - - - -, X', Y and Z1 are as defined in claim 1 and R5 represents an acid-labile protecting group.
22. A process according to claim 21 for the preparation of prostaglandin analogues of general formula II shown in claim 1, wherein R' represents an alkyl group, - - - - - represents a vinylene group,
X1 represents a trans-vinylene group, Y' represents a carbonyl group, and Y2 represents a hydroxy- methylene group which comprises the acid hydrolysis of a compound of the general formula:
(wherein A', A2, R2, R3, R4 and Z' are as defined in claim 1, R5 is as defined in claim 21 and R6 represents a straight- or branched-chain alkyl group containing from 1 to 6 carbon atoms.
23. A process for the preparation of a prostaglandin analogue of general formula II shown in claim 1 in the (5Z)-configuration which comprises the ultra-violet irradiation of a corresponding prostaglandin analogue of general formula II shown in claim 1 in the (SE)-configuration.
24. A process according to claim 21,22 or 23 followed by the conversion by methods known per se of a prostaglandin analogue of formula II into a corresponding compound of formula II wherein Al, A2, R', R2, R3, R4, - - - - -, X1, Y1, Y2 and Z' are as defined in claim 1, but wherein one or more of the symbols R', - - - --, - X', Y1 and Y2 have the following significances: R' represents a hydrogen atom;, - - - - - represents an ethylene group; X1 represents an ethylene group; Y' represents a hydroxy
methylene group; Y2 represents a carbonyl group; or the conversion of a prostaglandin analogue of general formula II wherein R1 represents a hydrogen atom into a non-toxic salt or alkyl ester thereof, or the conversion of a prostaglandin analogue of general formula II into a cyclodextrin clathrate thereof.
25. A process according to any one of claims 21 to 24 substantially as hereinfore described with especial reference to any one of the foregoing Examples.
26. A prostaglandin analogue of general formula II shown in claim 1 or, when R' represents a hydrogen atom, a non-toxic salt thereof or a cyclodextrin clathrate thereof, when prepared by a process claimed in any one of claims 21 to 25.
27. A pharmaceutical composition which comprises, as active ingredient, a prostaglandin analogue according to any one of claims 1 to 20 and 26, or a non-toxic salt or cyclodextrin clathrate thereof, in association with pharmaceutical carrier or coating.
28. A prostaglandin analogue of general formula II shown in claim 1, or, when R1 represents a hydrogen atom, a non-toxic salt thereof, or cyclodextrin clathrate thereof, for use in therapy.
29. A process for the preparation of compounds of the general formula:
wherein R'4 represents an acyl group which comprises the oxidation of a compound of the general formula:
wherein R'4 is as hereinbefore defined.
30. A process according to claim 29 in which the oxidation is effected using a thallium(lll) salt or by means of hydrogen peroxide in the presence of selenium dioxide.
31. A process according to claim 29 or 30 substantially as hereinbefore described.
32. A compound of general formula XII shown in claim 29, wherein R'4 is as defined in claim 29.
33. A compound of general formula XII shown in claim 29 wherein R'4 is as defined in claim 29 when prepared by the process claimed in claim 29, or 31.
34. A dialkylphosphonate salt of the general formula: [M']+[(R'50)2P(O)CHCO(CH2)4CH3]- XIV (wherein M' represents an alkali metal atom and R'5 represents an alkyl group of from 1 to 4 carbon atoms) in solid, isolated, form.
35. A dialkylphosphonate salt according to claim 34 wherein M' represents a sodium atom.
36. A dialkylphosphonate salt according to claim 34 or 35 wherein R'5 represents a methyl group.
37. A process for the conversion by known methods of a formyl group to a 3-oxooct-1 -enyl group using a dialkylphosphonate salt as claimed in claim 34, 35 or 36.
38. A process for the preparation of a compound of the general formula:
(wherein R'3 represents a protected oxygen function) which comprises the reaction of a compound of the general formula:
(wherein R16 is as hereinbefore defined) with a compound of general formula XIV (wherein M' and R15 are as defined in claim 34), the compound of general formula XIV used being as claimed in claim 34 and not prepared in situ.
39. A process according to claim 37 or 38 substantially as hereinbefore described.
40. A prostaglandin compound having a 3-oxooct-1 -enyl, 3-hydroxyoct-1 -enyl, 3-oxooctyl or 3hydroxyoctyl sidechain when prepared by a process using a compound of general formula XIV as claimed in claim 34 and not prepared in situ.
41. A prostaglandin compound according to claim 40 which is PGE" PGE2, PGF2Xr or carbocyclin.
42. A prostaglandin analogue according to claim 40 which is a prostaglandin analogue of general formula II.
43. A process for the preparation of a compound of the general formula:
(wherein A', A2, R2, R3, R4 and Z' are as defined in claim 1, R5 is as defined in claim 21 and Re is as defined in claim 22) which comprises the reaction of a compound of the general formula:
(wherein A2, R4 and Z' are as defined in claim 1 and R5 is as defined in claim 21) with a compound of the general formula: O=C(R2)A'CH(R3)COOR6 XX (wherein A', R2 and R3 are as defined in claim 1 and R6 is as defined in claim 22) in the presence of a compound of the general formula: M2[(R'7)3Si]2N XXI wherein M2 represents an alkali metal atom and R'7 represents an alkyl group of from 1 to 4 carbon atoms.
44. A process according to claim 43 in which M2 represents a lithium atom and R'7 represents a methyl group.
45. A process according to claim 43 substantially as hereinbefore described.
46. A compound of general formula XVIII (wherein Ai, A2, R2, R3, R4 and Z1 are as defined in claim 1, R5 is as defined in claim 21 and R6 is as defined in claim 22), when prepared by a process as claimed in claim 43, 44 or 45.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB08314615A GB2121410B (en) | 1982-05-28 | 1983-05-26 | 9a-oxo and hydroxy carbacyclins |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB8215742 | 1982-05-28 | ||
GB08314615A GB2121410B (en) | 1982-05-28 | 1983-05-26 | 9a-oxo and hydroxy carbacyclins |
Publications (3)
Publication Number | Publication Date |
---|---|
GB8314615D0 GB8314615D0 (en) | 1983-06-29 |
GB2121410A true GB2121410A (en) | 1983-12-21 |
GB2121410B GB2121410B (en) | 1985-09-04 |
Family
ID=26282987
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB08314615A Expired GB2121410B (en) | 1982-05-28 | 1983-05-26 | 9a-oxo and hydroxy carbacyclins |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB2121410B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2140418A (en) * | 1983-05-24 | 1984-11-28 | May & Baker Ltd | Carbacyclin analogues |
FR2547818A1 (en) * | 1983-05-24 | 1984-12-28 | May & Baker Ltd | New 6,9-methano-prostanoic acid derivs. |
-
1983
- 1983-05-26 GB GB08314615A patent/GB2121410B/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2140418A (en) * | 1983-05-24 | 1984-11-28 | May & Baker Ltd | Carbacyclin analogues |
FR2547818A1 (en) * | 1983-05-24 | 1984-12-28 | May & Baker Ltd | New 6,9-methano-prostanoic acid derivs. |
Also Published As
Publication number | Publication date |
---|---|
GB2121410B (en) | 1985-09-04 |
GB8314615D0 (en) | 1983-06-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4567195A (en) | Azaprostacyclins, their preparation and pharmaceutical use | |
CA1264320A (en) | Prostaglandin analogues, process for their preparation and pharmaceutical compositions containing them | |
JPS6147459A (en) | 11-substituted-16-phenoxyprostatrienoic acid derivative | |
GB2099814A (en) | 15-deoxy-16-hydroxy prostaglandins | |
JPH06145085A (en) | Carbacyclin analogue | |
US3751463A (en) | Cyclopentane derivatives | |
US4178367A (en) | Prostaglandin I2 analogues | |
AU6475900A (en) | Prostaglandin derivatives | |
US3932479A (en) | Lithium 3-triphenylmethoxy-1-trans-alkenyl-dialkyl alanates | |
US4024174A (en) | Tranes-delta 2-prostaglandins | |
US4034003A (en) | 15-Cycloalkyl-prostaglandins | |
US4294849A (en) | Prostaglandin analogues | |
JPH0368849B2 (en) | ||
US4517202A (en) | Prostaglandin analogues | |
GB2121410A (en) | 9 alpha -oxo and hydroxy carbacyclins | |
JPS5922699B2 (en) | Prostaglandin-like compounds | |
GB2048254A (en) | 19,20-didehydro, 19-hydroxy and 19-oxo-prostaglandin derivatives | |
US4698363A (en) | Prostacyclin analogues | |
JPS6034931A (en) | Prostaglandins | |
JPS61227544A (en) | Novel (4,2,0)bicyclooctane derivative | |
JPS6126970B2 (en) | ||
GB2140418A (en) | Carbacyclin analogues | |
US4123456A (en) | Novel 11-hydroxy-9-keto-5,6-cis-13,14-cis-prostadienoic acid derivatives | |
JPS6032763A (en) | Novel 11-oxoprostanoic acid derivative and its use | |
CA1098125A (en) | Process for the preparation of new prostaglandin analogues |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PCNP | Patent ceased through non-payment of renewal fee |