GB1540728A - Polyiodothyronine immunoassay - Google Patents

Polyiodothyronine immunoassay

Info

Publication number
GB1540728A
GB1540728A GB54276A GB54276A GB1540728A GB 1540728 A GB1540728 A GB 1540728A GB 54276 A GB54276 A GB 54276A GB 54276 A GB54276 A GB 54276A GB 1540728 A GB1540728 A GB 1540728A
Authority
GB
United Kingdom
Prior art keywords
polyiodothyronine
enzyme
medium
inhibited
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB54276A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syva Co
Original Assignee
Syva Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syva Co filed Critical Syva Co
Publication of GB1540728A publication Critical patent/GB1540728A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors

Abstract

Physiological amounts of polyiodothyronine in a medium, e.g. serum, are detected or determined by a homogeneous enzyme immunoanalysis. To this end, an enzyme is used which for the most part is reversibly inhibited by polyiodothyronine bound thereto. The medium, the inhibited enzyme and an antibody for polyiodothyronine are brought together in an aqueous zone which contains a substrate for the enzyme. The antibody is employed in an amount such that it substantially increases the enzyme activity of the inhibited enzyme if no free polyiodothyronine is present. If the medium added contains free polyiodothyronine, the increase in the enzyme activity is lower. By determination of the enzyme activity in the aqueous zone, the polyiodothyronine content of the medium can therefore be determined.
GB54276A 1975-02-20 1976-01-07 Polyiodothyronine immunoassay Expired GB1540728A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US55156675A 1975-02-20 1975-02-20

Publications (1)

Publication Number Publication Date
GB1540728A true GB1540728A (en) 1979-02-14

Family

ID=24201792

Family Applications (1)

Application Number Title Priority Date Filing Date
GB54276A Expired GB1540728A (en) 1975-02-20 1976-01-07 Polyiodothyronine immunoassay

Country Status (7)

Country Link
JP (1) JPS5924386B2 (en)
CA (1) CA1028643A (en)
CH (1) CH632343A5 (en)
DE (2) DE2660911C2 (en)
FR (1) FR2301823A1 (en)
GB (1) GB1540728A (en)
IT (1) IT1052946B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8912010B2 (en) 2011-09-26 2014-12-16 Fujifilm Corporation Thyroxine immunoassay using fluorescent particles

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4069105A (en) * 1977-03-03 1978-01-17 Syva Company Lidocaine antigens and antibodies
IT1153999B (en) * 1977-03-15 1987-01-21 Snam Progetti COMPOSITION SUITABLE FOR DETERMINING TRIIODOTHYRONIN AND DIAGNOSTIC METHOD USING THE SAME
US4156081A (en) * 1977-04-15 1979-05-22 Syva Company Theophylline antigens and antibodies
GB2102946B (en) * 1981-06-30 1984-11-28 Wellcome Found Enzyme immunoassay
EP0131096A2 (en) * 1983-05-02 1985-01-16 Miles Laboratories, Inc. Homogeneous immunoassay method for determining binding substances and reagent system therefor
DE3889407T2 (en) * 1987-10-02 1994-11-03 Microgenics Corp Homogeneous method for determining T-3 or T-4 uptake and composition therefor.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3551296A (en) * 1968-06-11 1970-12-29 Calbiochem Reagent and method for assaying malate dehydrogenase
IL39323A (en) * 1971-05-14 1975-08-31 Syva Co Highly sensitive assay method employing an enzyme for amplification
US3852157A (en) * 1971-05-14 1974-12-03 Syva Corp Compounds for enzyme amplification assay
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8912010B2 (en) 2011-09-26 2014-12-16 Fujifilm Corporation Thyroxine immunoassay using fluorescent particles

Also Published As

Publication number Publication date
CA1028643A (en) 1978-03-28
JPS5924386B2 (en) 1984-06-08
FR2301823A1 (en) 1976-09-17
DE2605162C2 (en) 1986-08-28
DE2605162A1 (en) 1976-09-02
FR2301823B1 (en) 1980-05-23
CH632343A5 (en) 1982-09-30
DE2660911C2 (en) 1986-05-28
JPS51106724A (en) 1976-09-21
IT1052946B (en) 1981-08-31

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19950107