GB1522559A - Enzyme purification - Google Patents
Enzyme purificationInfo
- Publication number
- GB1522559A GB1522559A GB2195176A GB2195176A GB1522559A GB 1522559 A GB1522559 A GB 1522559A GB 2195176 A GB2195176 A GB 2195176A GB 2195176 A GB2195176 A GB 2195176A GB 1522559 A GB1522559 A GB 1522559A
- Authority
- GB
- United Kingdom
- Prior art keywords
- column
- buffer
- gel
- mucor miehei
- coupled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1522559 Purifying ensigns NOVO INDUSTRI AS 29 April 1977 [27 May 1976] 21951/76 Heading C3H A process for the purification of Mucor miehei protease comprises applying a solution of Mucor miehei protease to an equilibrated column, prepared by transferring a coupled gel to a chromatography column, equilibrating the gel with a buffer having a pH between 5À0 and 6À0, rinsing the column with a buffer having a pH in the range of from 5À0 to 6À0, eluating the column with an eluating agent, which is buffer having a pH in the range of from 5À0 to 6À0, in which from 15 to 30% of an organic solvent is dissolved, and isolating pure Mucor miehei protease from the eluate, the coupled gel comprising CNBr-activated agarose gel beads coupled to a ligand prepared by the reaction of 1-chloro- 2,4-dinitrobenzene with hexamethylenediamine.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2195176A GB1522559A (en) | 1976-05-27 | 1976-05-27 | Enzyme purification |
BE175319A BE851896A (en) | 1976-05-27 | 1977-02-28 | IMPROVEMENTS RELATING TO THE PURIFICATION OF ENZYMES |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB2195176A GB1522559A (en) | 1976-05-27 | 1976-05-27 | Enzyme purification |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1522559A true GB1522559A (en) | 1978-08-23 |
Family
ID=10171501
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB2195176A Expired GB1522559A (en) | 1976-05-27 | 1976-05-27 | Enzyme purification |
Country Status (2)
Country | Link |
---|---|
BE (1) | BE851896A (en) |
GB (1) | GB1522559A (en) |
-
1976
- 1976-05-27 GB GB2195176A patent/GB1522559A/en not_active Expired
-
1977
- 1977-02-28 BE BE175319A patent/BE851896A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
BE851896A (en) | 1977-06-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
416 | Proceeding under section 16 patents act 1949 | ||
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19930429 |