GB1459320A - Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation - Google Patents

Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation

Info

Publication number
GB1459320A
GB1459320A GB151374A GB151374A GB1459320A GB 1459320 A GB1459320 A GB 1459320A GB 151374 A GB151374 A GB 151374A GB 151374 A GB151374 A GB 151374A GB 1459320 A GB1459320 A GB 1459320A
Authority
GB
United Kingdom
Prior art keywords
sample
separation
zone
terminating
leading
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB151374A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
LKB Produkter AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LKB Produkter AB filed Critical LKB Produkter AB
Publication of GB1459320A publication Critical patent/GB1459320A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1459320 Isotachophoretic separation LKB-PRODUKTER AB 11 Jan 1974 [15 Jan 1973] 01513/74 Heading C7B In a method of isotachophoretic separation of anions C 1 , C 2 , C 3 in a sample S between leading the terminating anions A and B in respective electrolytes L and T, to detect spectrophotometrically within a wavelength range, preferably within the UV range, the zone boundaries obtained, the counter-ions R utilized within the sample are absorptive to radiation in the wavelength range and are dispersed in the sample with a local concentration depending on the pH value in the sample in the locality. After separation, the absorptivity of a zone within the sample to radiation in the wavelength range depends on the pH value of the zone. In an example in which the leading electrolyte is 0À01 MCl-, the terminating electrolyte is capronate, and the sample ions to be separated are chlorate, oxalate, tartrate, citrate and acetate, the counterion is creatinine instead of #-alanine. In another example in which the leading and terminating electrolytes are respectively (CH 3 ) 4 NCl and Zn acetate in methanol, the counter-ion is sulphanilic acid.
GB151374A 1973-01-15 1974-01-11 Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation Expired GB1459320A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE7300492A SE369279B (en) 1973-01-15 1973-01-15

Publications (1)

Publication Number Publication Date
GB1459320A true GB1459320A (en) 1976-12-22

Family

ID=20316308

Family Applications (1)

Application Number Title Priority Date Filing Date
GB151374A Expired GB1459320A (en) 1973-01-15 1974-01-11 Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation

Country Status (6)

Country Link
US (1) US3912609A (en)
JP (1) JPS49105598A (en)
DE (1) DE2401620C3 (en)
FR (1) FR2214126B1 (en)
GB (1) GB1459320A (en)
SE (1) SE369279B (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4139440A (en) * 1977-06-20 1979-02-13 Government Of The United States Electrofocusing in buffers
JPS55156851A (en) * 1979-05-25 1980-12-06 Olympus Optical Co Ltd Fractionation processing method for electrophoresis
DE3603920A1 (en) * 1985-02-07 1986-08-07 Olympus Optical Co., Ltd., Tokio/Tokyo METHOD FOR RECORDING OR DISPLAYING IMAGES CREATED BY ELECTROPHORESIS
JPH065227B2 (en) * 1985-02-27 1994-01-19 オリンパス光学工業株式会社 Method for quantifying total protein value by electrophoresis
US5156724A (en) * 1990-01-29 1992-10-20 Millipore Corporation Method for analyzing ionic species using capillary electrophoresis
US8414754B1 (en) * 2006-05-31 2013-04-09 The Board Of Trustees Of The Leland Stanford Junior University Electrophoretic sample analysis and approach therefor
CA3012680A1 (en) 2016-01-29 2017-08-03 Purigen Biosystems, Inc. Isotachophoresis for purification of nucleic acids
CN111432919B (en) 2017-08-02 2022-08-02 普瑞珍生物***公司 Systems, devices, and methods for isotachophoresis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2962426A (en) * 1958-09-09 1960-11-29 Cambridge Instr Company Inc Electrochemical method for analyzing materials
SE339122B (en) * 1968-05-10 1971-09-27 Lkb Produkter Ab
GB1333796A (en) * 1970-06-02 1973-10-17 Lkb Produkter Ab Electrophoresis

Also Published As

Publication number Publication date
FR2214126B1 (en) 1980-01-04
DE2401620A1 (en) 1974-07-25
US3912609A (en) 1975-10-14
SE369279B (en) 1974-08-19
DE2401620B2 (en) 1975-02-20
FR2214126A1 (en) 1974-08-09
DE2401620C3 (en) 1975-10-02
JPS49105598A (en) 1974-10-05

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee