GB1459320A - Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation - Google Patents
Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separationInfo
- Publication number
- GB1459320A GB1459320A GB151374A GB151374A GB1459320A GB 1459320 A GB1459320 A GB 1459320A GB 151374 A GB151374 A GB 151374A GB 151374 A GB151374 A GB 151374A GB 1459320 A GB1459320 A GB 1459320A
- Authority
- GB
- United Kingdom
- Prior art keywords
- sample
- separation
- zone
- terminating
- leading
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1459320 Isotachophoretic separation LKB-PRODUKTER AB 11 Jan 1974 [15 Jan 1973] 01513/74 Heading C7B In a method of isotachophoretic separation of anions C 1 , C 2 , C 3 in a sample S between leading the terminating anions A and B in respective electrolytes L and T, to detect spectrophotometrically within a wavelength range, preferably within the UV range, the zone boundaries obtained, the counter-ions R utilized within the sample are absorptive to radiation in the wavelength range and are dispersed in the sample with a local concentration depending on the pH value in the sample in the locality. After separation, the absorptivity of a zone within the sample to radiation in the wavelength range depends on the pH value of the zone. In an example in which the leading electrolyte is 0À01 MCl-, the terminating electrolyte is capronate, and the sample ions to be separated are chlorate, oxalate, tartrate, citrate and acetate, the counterion is creatinine instead of #-alanine. In another example in which the leading and terminating electrolytes are respectively (CH 3 ) 4 NCl and Zn acetate in methanol, the counter-ion is sulphanilic acid.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE7300492A SE369279B (en) | 1973-01-15 | 1973-01-15 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1459320A true GB1459320A (en) | 1976-12-22 |
Family
ID=20316308
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB151374A Expired GB1459320A (en) | 1973-01-15 | 1974-01-11 | Method of spectrophotometrically detecting the zone boundaries obtained within a sample during an isotachophoretic separation |
Country Status (6)
Country | Link |
---|---|
US (1) | US3912609A (en) |
JP (1) | JPS49105598A (en) |
DE (1) | DE2401620C3 (en) |
FR (1) | FR2214126B1 (en) |
GB (1) | GB1459320A (en) |
SE (1) | SE369279B (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4139440A (en) * | 1977-06-20 | 1979-02-13 | Government Of The United States | Electrofocusing in buffers |
JPS55156851A (en) * | 1979-05-25 | 1980-12-06 | Olympus Optical Co Ltd | Fractionation processing method for electrophoresis |
DE3603920A1 (en) * | 1985-02-07 | 1986-08-07 | Olympus Optical Co., Ltd., Tokio/Tokyo | METHOD FOR RECORDING OR DISPLAYING IMAGES CREATED BY ELECTROPHORESIS |
JPH065227B2 (en) * | 1985-02-27 | 1994-01-19 | オリンパス光学工業株式会社 | Method for quantifying total protein value by electrophoresis |
US5156724A (en) * | 1990-01-29 | 1992-10-20 | Millipore Corporation | Method for analyzing ionic species using capillary electrophoresis |
US8414754B1 (en) * | 2006-05-31 | 2013-04-09 | The Board Of Trustees Of The Leland Stanford Junior University | Electrophoretic sample analysis and approach therefor |
CA3012680A1 (en) | 2016-01-29 | 2017-08-03 | Purigen Biosystems, Inc. | Isotachophoresis for purification of nucleic acids |
CN111432919B (en) | 2017-08-02 | 2022-08-02 | 普瑞珍生物***公司 | Systems, devices, and methods for isotachophoresis |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2962426A (en) * | 1958-09-09 | 1960-11-29 | Cambridge Instr Company Inc | Electrochemical method for analyzing materials |
SE339122B (en) * | 1968-05-10 | 1971-09-27 | Lkb Produkter Ab | |
GB1333796A (en) * | 1970-06-02 | 1973-10-17 | Lkb Produkter Ab | Electrophoresis |
-
1973
- 1973-01-15 SE SE7300492A patent/SE369279B/xx unknown
-
1974
- 1974-01-11 GB GB151374A patent/GB1459320A/en not_active Expired
- 1974-01-14 FR FR7401192A patent/FR2214126B1/fr not_active Expired
- 1974-01-14 DE DE2401620A patent/DE2401620C3/en not_active Expired
- 1974-01-14 JP JP49007443A patent/JPS49105598A/ja active Pending
- 1974-01-14 US US432836A patent/US3912609A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
FR2214126B1 (en) | 1980-01-04 |
DE2401620A1 (en) | 1974-07-25 |
US3912609A (en) | 1975-10-14 |
SE369279B (en) | 1974-08-19 |
DE2401620B2 (en) | 1975-02-20 |
FR2214126A1 (en) | 1974-08-09 |
DE2401620C3 (en) | 1975-10-02 |
JPS49105598A (en) | 1974-10-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed [section 19, patents act 1949] | ||
PCNP | Patent ceased through non-payment of renewal fee |