GB1442677A - Microbial lipase - Google Patents
Microbial lipaseInfo
- Publication number
- GB1442677A GB1442677A GB4394373A GB4394373A GB1442677A GB 1442677 A GB1442677 A GB 1442677A GB 4394373 A GB4394373 A GB 4394373A GB 4394373 A GB4394373 A GB 4394373A GB 1442677 A GB1442677 A GB 1442677A
- Authority
- GB
- United Kingdom
- Prior art keywords
- lipase
- microbial
- bile
- activity
- sept
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1442677 Microbial lipase TAKEDA YAKUHIN KOGYO KK 19 Sept 1973 [20 Sept 1972] 43943/73 Heading C3H [Also in Divisions A5 C5 and G1] A microbial lipase, which accumulates in the culture medium during cultivation of microorganisms of the genus Phycomyces, particularly P. nitens and P. blakesleeanus, and whose enzyme activity is enhanced in the presence of bile, is characterized as follows: maximum ultraviolet absorption spectrum at 275-280 millimicrons, significant infra-red absorption spectra at 3À0, 3À38, 6À05, 6À55, 6À88, 7À15, 8À12, and 9À3 microns, soluble in water and aqueous salt solution of 0À01 to 0À1 of ionic strength, insoluble in alcohols, acetone and ether, optional activity between pH 5 and 8 and 35-41‹ C., stable over pH range 4 to 10 at 23‹ C., stable at temperatures up to 40‹ C. but inactivated by 30% at 45‹ C. and almost completely inactivated at 50‹ C. at pH 7, not inhibited by diisopropyl fluorophosphate, EDTA or p-chloromercuribenzoate (specified conditions) and exhibits an at least 50% increase in lipase activity on olive oil in the presence of dog bile diluted to 1/200. Characteristics from amino acid analysis: no cysteine residue, two S-S bonds, two trytophan residues, no saccharide and no lipid. The enzyme is recovered from the culture medium and purified by conventional methods. The Specification discloses pharmaceutical preparations (see Division A5) including in combination (1) the lipase and bovine bile powder and (2) the lipase, microbial acid protease, amylase and cellulase, and pancreatin.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP47094222A JPS5017559B2 (en) | 1972-09-20 | 1972-09-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1442677A true GB1442677A (en) | 1976-07-14 |
Family
ID=14104274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4394373A Expired GB1442677A (en) | 1972-09-20 | 1973-09-19 | Microbial lipase |
Country Status (19)
| Country | Link |
|---|---|
| JP (1) | JPS5017559B2 (en) |
| AT (1) | AT322489B (en) |
| BE (1) | BE804921A (en) |
| CA (1) | CA996045A (en) |
| CH (1) | CH585792A5 (en) |
| CS (1) | CS185629B2 (en) |
| DE (1) | DE2346335A1 (en) |
| DK (1) | DK135384B (en) |
| ES (1) | ES418898A1 (en) |
| FI (1) | FI51951C (en) |
| FR (1) | FR2199974B1 (en) |
| GB (1) | GB1442677A (en) |
| HU (1) | HU169794B (en) |
| IT (1) | IT1014023B (en) |
| NL (1) | NL7312936A (en) |
| NO (1) | NO139353C (en) |
| PH (1) | PH13554A (en) |
| PL (1) | PL91581B1 (en) |
| SE (1) | SE396963B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0317355A2 (en) * | 1987-11-19 | 1989-05-24 | Oklahoma Medical Research Foundation | Dietary compositions and methods using bile salt-activated lipase |
| EP0968720A2 (en) * | 1998-07-02 | 2000-01-05 | Amano Pharmaceutical Co., Ltd. | Gastric emptying-promoting composition |
| EP1149585A1 (en) * | 2000-04-27 | 2001-10-31 | Warner-Lambert Company | Use of recombinant gastric lipase for treating functional dyspepsia |
| US7718169B2 (en) | 2004-10-14 | 2010-05-18 | Cystic Fibrosis Foundations Therapeutics, Inc. | Compositions and methods for treating pancreatic insufficiency |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3131921B1 (en) * | 2014-04-15 | 2020-06-10 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
| TWI662131B (en) * | 2018-11-27 | 2019-06-11 | 台灣中油股份有限公司 | Method for preparing rice bran lipase |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1332663A (en) * | 1969-10-14 | 1973-10-03 | Sugiura M | Lysosome labilizer preparation thereof and compositions including the same |
-
1972
- 1972-09-20 JP JP47094222A patent/JPS5017559B2/ja not_active Expired
-
1973
- 1973-09-06 NO NO349673A patent/NO139353C/en unknown
- 1973-09-14 DE DE19732346335 patent/DE2346335A1/en active Pending
- 1973-09-17 BE BE135703A patent/BE804921A/en not_active IP Right Cessation
- 1973-09-17 PH PH15029A patent/PH13554A/en unknown
- 1973-09-17 FI FI288073A patent/FI51951C/en active
- 1973-09-17 CS CS639473A patent/CS185629B2/en unknown
- 1973-09-17 HU HUTA001269 patent/HU169794B/hu unknown
- 1973-09-18 CH CH1337173A patent/CH585792A5/xx not_active IP Right Cessation
- 1973-09-19 GB GB4394373A patent/GB1442677A/en not_active Expired
- 1973-09-19 DK DK511573A patent/DK135384B/en unknown
- 1973-09-19 ES ES418898A patent/ES418898A1/en not_active Expired
- 1973-09-19 IT IT6977173A patent/IT1014023B/en active
- 1973-09-19 FR FR7333640A patent/FR2199974B1/fr not_active Expired
- 1973-09-19 CA CA181,452A patent/CA996045A/en not_active Expired
- 1973-09-19 SE SE7312777A patent/SE396963B/en unknown
- 1973-09-19 NL NL7312936A patent/NL7312936A/xx unknown
- 1973-09-20 AT AT809873A patent/AT322489B/en not_active IP Right Cessation
- 1973-09-20 PL PL16532773A patent/PL91581B1/pl unknown
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0317355A2 (en) * | 1987-11-19 | 1989-05-24 | Oklahoma Medical Research Foundation | Dietary compositions and methods using bile salt-activated lipase |
| EP0317355B1 (en) * | 1987-11-19 | 1998-07-08 | Oklahoma Medical Research Foundation | Dietary compositions and methods using bile salt-activated lipase |
| EP0968720A2 (en) * | 1998-07-02 | 2000-01-05 | Amano Pharmaceutical Co., Ltd. | Gastric emptying-promoting composition |
| EP0968720A3 (en) * | 1998-07-02 | 2003-01-22 | Amano Enzyme Inc. | Gastric emptying-promoting composition |
| US6656464B2 (en) | 1998-07-02 | 2003-12-02 | Amano Pharmaceutical Co., Ltd. | Gastric emptying-promoting composition |
| EP1149585A1 (en) * | 2000-04-27 | 2001-10-31 | Warner-Lambert Company | Use of recombinant gastric lipase for treating functional dyspepsia |
| WO2001080878A1 (en) * | 2000-04-27 | 2001-11-01 | Meristem Therapeutics | Use of recombinant gastric lipase for treating functional dyspepsia |
| US7718169B2 (en) | 2004-10-14 | 2010-05-18 | Cystic Fibrosis Foundations Therapeutics, Inc. | Compositions and methods for treating pancreatic insufficiency |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2199974B1 (en) | 1977-01-28 |
| NO139353B (en) | 1978-11-13 |
| ES418898A1 (en) | 1976-03-01 |
| DK135384C (en) | 1977-10-03 |
| PL91581B1 (en) | 1977-03-31 |
| FI51951B (en) | 1977-01-31 |
| DE2346335A1 (en) | 1974-03-28 |
| JPS5017559B2 (en) | 1975-06-21 |
| CA996045A (en) | 1976-08-31 |
| JPS4948887A (en) | 1974-05-11 |
| FI51951C (en) | 1977-05-10 |
| CS185629B2 (en) | 1978-10-31 |
| PH13554A (en) | 1980-06-26 |
| BE804921A (en) | 1974-03-18 |
| DK135384B (en) | 1977-04-18 |
| IT1014023B (en) | 1977-04-20 |
| NL7312936A (en) | 1974-03-22 |
| NO139353C (en) | 1979-02-21 |
| AT322489B (en) | 1975-05-26 |
| AU6040573A (en) | 1975-03-20 |
| SE396963B (en) | 1977-10-10 |
| CH585792A5 (en) | 1977-03-15 |
| FR2199974A1 (en) | 1974-04-19 |
| HU169794B (en) | 1977-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |