GB1414235A - Method for purifying cyclodextrin-producing enzymes - Google Patents

Method for purifying cyclodextrin-producing enzymes

Info

Publication number
GB1414235A
GB1414235A GB5038173A GB5038173A GB1414235A GB 1414235 A GB1414235 A GB 1414235A GB 5038173 A GB5038173 A GB 5038173A GB 5038173 A GB5038173 A GB 5038173A GB 1414235 A GB1414235 A GB 1414235A
Authority
GB
United Kingdom
Prior art keywords
starch
enzyme
solution
cyclodextrin
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5038173A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hayashibara Kk Okada S
KITAHATA S
Original Assignee
Hayashibara Kk Okada S
KITAHATA S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hayashibara Kk Okada S, KITAHATA S filed Critical Hayashibara Kk Okada S
Publication of GB1414235A publication Critical patent/GB1414235A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

1414235 Purifying cyclodextrin-producing enzymes HAYASHIBARA KK S OKADA and S KITAHATA 30 Oct 1973 [30 Oct 1972] 50381/73 Heading C3H A method of purifying a cyclodextrinproducing enzyme involves contacting an aqueous solution of the contaminated enzyme, e.g. the filtrate of a culture broth of Bacillus macerous or Bacillus megaterium, with starch and/or cyclodextrin (cycloheptadextrin, cyclohexadextrin or crude cyclodextrins) and then eluting the enzyme that has been absorbed thereon. In a preferred procedure, there is added to the initial impure solution ammonium sulphate in a concentration of 25-30% saturation or a hydrophilic organic solvent (ethanol, acetone or isopropanol) up to 30% v./v., as a result of which any co-existing protein is rendered turbid and may be filtered off, starch is introduced and the resulting starch suspension is passed through a layer or column of starch including a small amount of diatomaceous earth. The starch layer is washed with an aqueous solution of ammonium sulphate and/or a hydrophilic organic solvent, the washed starch is suspended in dilute aqueous sodium hydrogen phosphate solution and a solution of the purified enzyme is recovered by filtration.
GB5038173A 1972-10-30 1973-10-30 Method for purifying cyclodextrin-producing enzymes Expired GB1414235A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP10869872A JPS5147795B2 (en) 1972-10-30 1972-10-30

Publications (1)

Publication Number Publication Date
GB1414235A true GB1414235A (en) 1975-11-19

Family

ID=14491358

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5038173A Expired GB1414235A (en) 1972-10-30 1973-10-30 Method for purifying cyclodextrin-producing enzymes

Country Status (2)

Country Link
JP (1) JPS5147795B2 (en)
GB (1) GB1414235A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0017242A1 (en) * 1979-04-07 1980-10-15 Sanraku-Ocean Co., Ltd. A process for producing cyclodextrins
EP0118547A1 (en) * 1982-09-13 1984-09-19 Forsyth Dental Infirmary Method for the preparation of glucosyltransferase.
GB2169902A (en) * 1984-12-03 1986-07-23 Hayashibara Biochem Lab Polypeptide possessing cyclomaltodextrin glucanotransferase activity
US5278059A (en) * 1985-12-04 1994-01-11 Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo Polypeptide possessing cyclomaltodextrin glucanotransferase activity

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0017242A1 (en) * 1979-04-07 1980-10-15 Sanraku-Ocean Co., Ltd. A process for producing cyclodextrins
EP0118547A1 (en) * 1982-09-13 1984-09-19 Forsyth Dental Infirmary Method for the preparation of glucosyltransferase.
EP0118547A4 (en) * 1982-09-13 1986-04-15 Forsyth Dental Infirmary Method for the preparation of glucosyltransferase.
GB2169902A (en) * 1984-12-03 1986-07-23 Hayashibara Biochem Lab Polypeptide possessing cyclomaltodextrin glucanotransferase activity
GB2169902B (en) * 1984-12-03 1989-09-06 Hayashibara Biochem Lab Polypeptide possessing cyclomaltodextrin glucanotransferase activity
US5278059A (en) * 1985-12-04 1994-01-11 Kabushiki Kaisha Hayashibara Seibutsu Kagaki Kenkyujo Polypeptide possessing cyclomaltodextrin glucanotransferase activity

Also Published As

Publication number Publication date
JPS4966884A (en) 1974-06-28
JPS5147795B2 (en) 1976-12-16

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee