GB1373106A - Triglyceride hydrolysis and assay - Google Patents
Triglyceride hydrolysis and assayInfo
- Publication number
- GB1373106A GB1373106A GB5560971A GB5560971A GB1373106A GB 1373106 A GB1373106 A GB 1373106A GB 5560971 A GB5560971 A GB 5560971A GB 5560971 A GB5560971 A GB 5560971A GB 1373106 A GB1373106 A GB 1373106A
- Authority
- GB
- United Kingdom
- Prior art keywords
- nadh
- protease
- glycerol
- lipase
- ldh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000003556 assay Methods 0.000 title abstract 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 title abstract 2
- 230000007062 hydrolysis Effects 0.000 title 1
- 238000006460 hydrolysis reaction Methods 0.000 title 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 7
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 abstract 7
- 239000004365 Protease Substances 0.000 abstract 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 5
- 108091005804 Peptidases Proteins 0.000 abstract 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 5
- 235000019419 proteases Nutrition 0.000 abstract 5
- 102000003855 L-lactate dehydrogenase Human genes 0.000 abstract 4
- 108700023483 L-lactate dehydrogenases Proteins 0.000 abstract 4
- 239000004367 Lipase Substances 0.000 abstract 4
- 102000004882 Lipase Human genes 0.000 abstract 4
- 108090001060 Lipase Proteins 0.000 abstract 4
- 235000019421 lipase Nutrition 0.000 abstract 4
- WVVOBOZHTQJXPB-UHFFFAOYSA-N N-anilino-N-nitronitramide Chemical compound [N+](=O)([O-])N(NC1=CC=CC=C1)[N+](=O)[O-] WVVOBOZHTQJXPB-UHFFFAOYSA-N 0.000 abstract 3
- 102000013009 Pyruvate Kinase Human genes 0.000 abstract 3
- 108020005115 Pyruvate Kinase Proteins 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 239000011541 reaction mixture Substances 0.000 abstract 3
- 102000006746 NADH Dehydrogenase Human genes 0.000 abstract 2
- 108010086428 NADH Dehydrogenase Proteins 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 abstract 2
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 230000003287 optical effect Effects 0.000 abstract 2
- DTBNBXWJWCWCIK-UHFFFAOYSA-K phosphonatoenolpyruvate Chemical compound [O-]C(=O)C(=C)OP([O-])([O-])=O DTBNBXWJWCWCIK-UHFFFAOYSA-K 0.000 abstract 2
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 abstract 1
- 108010004032 Bromelains Proteins 0.000 abstract 1
- 241000146387 Chromobacterium viscosum Species 0.000 abstract 1
- 108090000317 Chymotrypsin Proteins 0.000 abstract 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 abstract 1
- 102000057621 Glycerol kinases Human genes 0.000 abstract 1
- 108700016170 Glycerol kinases Proteins 0.000 abstract 1
- 102000000587 Glycerolphosphate Dehydrogenase Human genes 0.000 abstract 1
- 108010041921 Glycerolphosphate Dehydrogenase Proteins 0.000 abstract 1
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract 1
- 102000016387 Pancreatic elastase Human genes 0.000 abstract 1
- 108010067372 Pancreatic elastase Proteins 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 abstract 1
- 241000303962 Rhizopus delemar Species 0.000 abstract 1
- 241000187392 Streptomyces griseus Species 0.000 abstract 1
- 108090000631 Trypsin Proteins 0.000 abstract 1
- 102000004142 Trypsin Human genes 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 229960002376 chymotrypsin Drugs 0.000 abstract 1
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 1
- 229940120503 dihydroxyacetone Drugs 0.000 abstract 1
- 150000002148 esters Chemical class 0.000 abstract 1
- 229930195729 fatty acid Natural products 0.000 abstract 1
- 239000000194 fatty acid Substances 0.000 abstract 1
- 150000004665 fatty acids Chemical class 0.000 abstract 1
- -1 glycerol ester Chemical class 0.000 abstract 1
- 229910052739 hydrogen Inorganic materials 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 229910001425 magnesium ion Inorganic materials 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 abstract 1
- 210000004080 milk Anatomy 0.000 abstract 1
- 239000008267 milk Substances 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 125000003831 tetrazolyl group Chemical group 0.000 abstract 1
- 239000012588 trypsin Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/897—Streptomyces griseus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/939—Rhizopus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
1373106 Determination of triglyceride CALBIOCHEM 30 Nov 1971 [16 Dec 1970] 55609/71 Heading G1B A glycerol ester of a fatty acid in an aqueous liquid, for example serum or milk is assayed by hydrolyzing the ester by adding a lipase and a protease to the liquid, then determining the amount of glycerol present in the resulting solution. The lipase may be from Chromobacterium viscosum or Rhizopus delemar; the protease may be chymotrypsin (exemplified), trypsin, a streptomyces griseus protease, elastase, papain or bromelin. The assay may be carried out (a) using a reagent comprising the lipase and protease, glycerol kinase, ATP, phosphoenol pyruvate (PEP), a magnesium ion source, pyruvate kinase (PK), lactate dehydrogenase (LDH) and NADH and measuring the decrease in optical density resulting from the oxidation of the NADH; (b) using the reagent as in (a), without the NADH and LDH, and reacting the pyruvate ion formed with dinitrophenyl hydrazine; (c) using the reagent as in (a), without the PEP, PK NADH and LDH, adding glycerol phosphate dehydrogenase and NAD, and measuring increase in fluorescence produced by formation of NADH, or optionally also including in the reaction mixture dinitrophenyl hydrazine which forms a coloured product with dihydroxyacetone phosphate; or (d) using a reaction mixture which comprises the lipase, protease, NAD and glycerol dehydrogenase and measuring the increase in optical density, resulting from formation of NADH, or alternatively adding dinitrophenylhydrazine to form a coloured product with the dihydroxyacetone in the reaction mixture. In other methods the NADH formed may be determined by reaction with a tetrazolium salt in the presence of a hydrogen transfer agent such as diaphorase or phenazine methosulphate to form a coloured dye.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9890470A | 1970-12-16 | 1970-12-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1373106A true GB1373106A (en) | 1974-11-06 |
Family
ID=22271481
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB5560971A Expired GB1373106A (en) | 1970-12-16 | 1971-11-30 | Triglyceride hydrolysis and assay |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US3703591A (en) |
| JP (2) | JPS549518B1 (en) |
| AU (1) | AU475955B2 (en) |
| BE (1) | BE776034A (en) |
| BR (1) | BR7108185D0 (en) |
| CA (1) | CA955161A (en) |
| CH (2) | CH563404A5 (en) |
| CS (1) | CS212729B2 (en) |
| DE (1) | DE2162325C3 (en) |
| ES (1) | ES395956A1 (en) |
| FR (1) | FR2118454A5 (en) |
| GB (1) | GB1373106A (en) |
| IL (1) | IL38235A (en) |
| IT (1) | IT972079B (en) |
| NL (1) | NL180523C (en) |
| SE (1) | SE389919B (en) |
| ZA (1) | ZA717957B (en) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2101796A1 (en) * | 1970-01-21 | 1971-08-05 | Baxter Laboratories Inc | Method for the determination of glycene in blood serum |
| AT324282B (en) * | 1972-06-19 | 1975-08-25 | Boehringer Mannheim Gmbh | METHOD AND REAGENT FOR DETERMINATION OF TRIGLYCERIDES |
| CS164231B2 (en) * | 1972-09-28 | 1975-11-07 | ||
| JPS49113695A (en) * | 1973-02-27 | 1974-10-30 | ||
| AT339505B (en) * | 1974-03-14 | 1977-10-25 | Boehringer Mannheim Gmbh | ENZYMATIC ANALYSIS PROCEDURE |
| US3898130A (en) * | 1974-03-18 | 1975-08-05 | American Hospital Supply Corp | Rapid enzymatic hydrolysis of triglycerides |
| CA1029644A (en) * | 1974-03-20 | 1978-04-18 | The Dow Chemical Company | Method for determination of triglycerides and glycerol |
| CA1056282A (en) * | 1974-03-25 | 1979-06-12 | Charles T. Goodhue | Multilayer analytical elements for use in the assay of cholesterol |
| US3884764A (en) * | 1974-03-25 | 1975-05-20 | Eastman Kodak Co | Method and composition for blood serum cholesterol analysis |
| JPS50142090A (en) * | 1974-04-30 | 1975-11-15 | ||
| JPS5169691A (en) * | 1974-06-07 | 1976-06-16 | Iatron Lab | Ketsuseichuno chuseishibosokuteiyoshaku |
| US4014744A (en) * | 1975-01-30 | 1977-03-29 | Miles Laboratories Inc. | Processes for measuring tri-, di- and monoglycerides |
| US4011045A (en) * | 1975-02-14 | 1977-03-08 | Bonderman Dean P | Turbidity reduction in triglyceride standards |
| JPS5217085A (en) * | 1975-07-30 | 1977-02-08 | Ono Pharmaceut Co Ltd | Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes |
| US4012287A (en) * | 1975-11-18 | 1977-03-15 | Dr. Bruno Lange Gmbh | Method and reagent for the quantitative analysis of triglycerides |
| US4045297A (en) * | 1975-12-15 | 1977-08-30 | Monsanto Company | Triglycerides determination method |
| CA1100023A (en) * | 1976-08-19 | 1981-04-28 | Charles T. Goodhue | Process and composition for the quantification of glycerol and triglycerides |
| CA1114269A (en) * | 1976-08-19 | 1981-12-15 | Charles D. Warburton | Integral element for the detection of glycerol or triglycerides |
| US4275151A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| US4275152A (en) * | 1977-02-03 | 1981-06-23 | Eastman Kodak Company | Hydrolysis of protein-bound cholesterol esters |
| JPS6058746B2 (en) * | 1977-09-22 | 1985-12-21 | 中外製薬株式会社 | Higher fatty acid ester |
| US4195126A (en) * | 1977-10-04 | 1980-03-25 | The Board Of Trustees Of The University Of Alabama | Albumin-dye complex for fatty acid determination |
| US4245041A (en) * | 1977-12-07 | 1981-01-13 | American Monitor Corporation | Triglycerides assay and reagents therefor |
| US4241178A (en) * | 1978-01-06 | 1980-12-23 | Eastman Kodak Company | Process and composition for the quantification of glycerol ATP and triglycerides |
| US4223090A (en) * | 1978-07-13 | 1980-09-16 | American Hospital Supply Corporation | Reagents for the enzymatic determination of triglycerides |
| DE2831580C2 (en) * | 1978-07-18 | 1980-09-18 | Boehringer Mannheim Gmbh, 6800 Mannheim | Method and reagent for the determination of glycerin |
| DE2834704A1 (en) * | 1978-08-08 | 1980-02-21 | Boehringer Mannheim Gmbh | METHOD FOR THE QUANTITATIVE ENZYMATIC DETERMINATION OF ADP |
| US4302536A (en) * | 1978-08-15 | 1981-11-24 | Longenecker Robert W | Colorimetric immunoassay process |
| US4264589A (en) * | 1978-12-20 | 1981-04-28 | Felts James M | Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system |
| US4178285A (en) * | 1978-12-20 | 1979-12-11 | Felts James M | Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system |
| US4394445A (en) * | 1979-02-22 | 1983-07-19 | Nix Paul T | Enzymatic glyceride hydrolysis |
| US4259440A (en) * | 1979-05-21 | 1981-03-31 | Miles Laboratories, Inc. | Hydrolysis and assay of triglycerides |
| US4322496A (en) * | 1980-04-17 | 1982-03-30 | Eastman Kodak Company | Inhibition of lactate oxidase |
| US4309502A (en) * | 1980-06-30 | 1982-01-05 | Beckman Instruments, Inc. | Enzymatic assay for glycerol and triglycerides and a reagent for use therein |
| US4338395A (en) * | 1980-07-21 | 1982-07-06 | Technicon Instruments Corporation | Method for the analysis of triglycerides |
| EP0045031A1 (en) * | 1980-07-22 | 1982-02-03 | Baker Instruments Corporation | Glycerol detection reagent and its use |
| US4394444A (en) * | 1982-06-21 | 1983-07-19 | Miles Laboratories, Inc. | Cofactor indicator compositions |
| JPS59140900A (en) * | 1983-01-28 | 1984-08-13 | Toyo Jozo Co Ltd | Novel method for highly sensitive, enzymatic determination |
| JPS59196100A (en) * | 1983-04-21 | 1984-11-07 | Amano Pharmaceut Co Ltd | Glycerol determination and composition therefor |
| US4626511A (en) * | 1985-05-13 | 1986-12-02 | Wayne State University | Composition for reducing turbidity in samples of biological fluids |
| US5310679A (en) * | 1985-05-13 | 1994-05-10 | Artiss Joseph D | Composition for reducing turbidity in samples of biological fluids |
| EP1329512A4 (en) * | 2000-09-28 | 2004-09-01 | Arkray Inc | Process for producing protein decomposition product |
| JP4336886B2 (en) | 2001-09-28 | 2009-09-30 | アークレイ株式会社 | Tetrazolium compound storage method, stabilizer used therefor, and tetrazolium compound reagent solution using the method |
| WO2004059315A1 (en) * | 2002-12-24 | 2004-07-15 | Stepan Company | Method for determination of free and combined glycerin in biodiesel |
| US20100296087A1 (en) * | 2007-05-31 | 2010-11-25 | Juergen Gailer | Method for assessing trace element related disorders in blood plasma |
| CN102016073A (en) * | 2008-02-29 | 2011-04-13 | Isis创新有限公司 | Diagnostic methods |
| US9817001B2 (en) | 2008-05-27 | 2017-11-14 | Boston Heart Diagnostics Corporation | Methods for determining LDL cholesterol treatment |
| US8470541B1 (en) | 2008-09-27 | 2013-06-25 | Boston Heart Diagnostics Corporation | Methods for separation and immuno-detection of biomolecules, and apparatus related thereto |
| WO2013056087A2 (en) | 2011-10-13 | 2013-04-18 | Boston Heart Diagnostics | Compositions and methods for treating and preventing coronary heart disease |
| US20140120559A1 (en) | 2012-10-26 | 2014-05-01 | Boston Heart Diagnostics Corporation | Diabetes panel |
| US9828624B2 (en) | 2013-07-24 | 2017-11-28 | Boston Heart Diagnostics Corporation | Driving patient compliance with therapy |
| EP3220810A4 (en) | 2014-11-17 | 2018-05-16 | Boston Heart Diagnostic Corporation | Cardiovascular disease risk assessment |
-
1970
- 1970-12-16 US US98904A patent/US3703591A/en not_active Expired - Lifetime
-
1971
- 1971-10-13 ES ES295956A patent/ES395956A1/en not_active Expired
- 1971-11-12 FR FR7140669A patent/FR2118454A5/fr not_active Expired
- 1971-11-24 CA CA128,467A patent/CA955161A/en not_active Expired
- 1971-11-26 IL IL38235A patent/IL38235A/en unknown
- 1971-11-26 ZA ZA717957A patent/ZA717957B/en unknown
- 1971-11-26 CH CH202475A patent/CH563404A5/xx not_active IP Right Cessation
- 1971-11-26 CH CH1720671A patent/CH566003A5/xx not_active IP Right Cessation
- 1971-11-30 BE BE776034A patent/BE776034A/en not_active IP Right Cessation
- 1971-11-30 GB GB5560971A patent/GB1373106A/en not_active Expired
- 1971-12-02 AU AU36416/71A patent/AU475955B2/en not_active Expired
- 1971-12-09 BR BR8185/71A patent/BR7108185D0/en unknown
- 1971-12-15 DE DE2162325A patent/DE2162325C3/en not_active Expired
- 1971-12-15 JP JP10112371A patent/JPS549518B1/ja active Pending
- 1971-12-15 SE SE7116097A patent/SE389919B/en unknown
- 1971-12-15 IT IT54760/71A patent/IT972079B/en active
- 1971-12-16 CS CS718751A patent/CS212729B2/en unknown
- 1971-12-16 NL NLAANVRAGE7117275,A patent/NL180523C/en not_active IP Right Cessation
-
1978
- 1978-02-04 JP JP1106278A patent/JPS53114493A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL38235A0 (en) | 1972-01-27 |
| IL38235A (en) | 1974-10-22 |
| NL180523B (en) | 1986-10-01 |
| ZA717957B (en) | 1972-08-30 |
| CH563404A5 (en) | 1975-06-30 |
| CH566003A5 (en) | 1975-08-29 |
| AU475955B2 (en) | 1976-09-09 |
| NL7117275A (en) | 1972-06-20 |
| DE2162325A1 (en) | 1972-06-22 |
| ES395956A1 (en) | 1974-09-01 |
| JPS53114493A (en) | 1978-10-05 |
| CA955161A (en) | 1974-09-24 |
| FR2118454A5 (en) | 1972-07-28 |
| BE776034A (en) | 1972-03-16 |
| DE2162325B2 (en) | 1980-04-24 |
| CS212729B2 (en) | 1982-03-26 |
| NL180523C (en) | 1987-03-02 |
| US3703591A (en) | 1972-11-21 |
| AU3641671A (en) | 1973-06-07 |
| SE389919B (en) | 1976-11-22 |
| DE2162325C3 (en) | 1980-12-11 |
| BR7108185D0 (en) | 1973-05-31 |
| IT972079B (en) | 1974-05-20 |
| JPS549518B1 (en) | 1979-04-25 |
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