GB1108533A - Water-insoluble enzymes - Google Patents
Water-insoluble enzymesInfo
- Publication number
- GB1108533A GB1108533A GB12399/66A GB1239966A GB1108533A GB 1108533 A GB1108533 A GB 1108533A GB 12399/66 A GB12399/66 A GB 12399/66A GB 1239966 A GB1239966 A GB 1239966A GB 1108533 A GB1108533 A GB 1108533A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acid
- enzyme
- amino
- macromolecular compound
- norleucine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B11/00—Preparation of cellulose ethers
- C08B11/02—Alkyl or cycloalkyl ethers
- C08B11/04—Alkyl or cycloalkyl ethers with substituted hydrocarbon radicals
- C08B11/14—Alkyl or cycloalkyl ethers with substituted hydrocarbon radicals with nitrogen-containing groups
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Molecular Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
A water-insoluble enzyme comprises the reaction product of a bacterial acylase and a macromolecular compound having a functional group capable of coupling with an enzyme protein without substantially decreasing the activity thereof. The enzyme may be produced by cultivation of Micrococcus glutamicus or Pseudomonas cruciviae. The macromolecular compound may be polyaminostyrene, p-amino-benzyl cellulose, a nitrated copolymer of methacrylic acid and methacrylic acid-3-fluoroanilide or any compound containing aminophenyl, acid anhydride, acid chloride, isocyanate, acid azide or nitro-fluorophenyl groups.ALSO:Racemic amino acids are resolved by acylating the amino group and selectively hydrolysing the acyl group of the L-form with an enzyme which is rendered insoluble by reaction with a macromolecular compound. Acylated amino acids which may be so hydrolysed includ N-acetyl - L - methionine, - L - alanine, - L - valine, - L - leucine, - L - threonine, - L - norvaline and - L - norleucine, and N - chloroacetyl - L - isoleucine, - L - norleucine, - L - serine, - L - alanine, - L - phenylalanine and - L - valine. Other acid groups which may be removed are benzoyl, dichloroacetyl, chloropropionyl and formyl. The O-form remains unattacked and may be recovered and hydrolysed with acid to give the O-form of the amino acid.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1592965 | 1965-03-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1108533A true GB1108533A (en) | 1968-04-03 |
Family
ID=11902447
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB12399/66A Expired GB1108533A (en) | 1965-03-20 | 1966-03-21 | Water-insoluble enzymes |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE1517761A1 (en) |
GB (1) | GB1108533A (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2059566A1 (en) * | 1969-07-28 | 1971-06-04 | Cancer Res Center | |
FR2073443A1 (en) * | 1969-12-02 | 1971-10-01 | Baxter Laboratories Inc | |
FR2490241A1 (en) * | 1980-09-17 | 1982-03-19 | Grace W R Ltd | PROCESS FOR THE PREPARATION OF L-ASPARTIC ACID, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR USE AND METHOD FOR THE PREPARATION THEREOF |
FR2490240A1 (en) * | 1980-09-17 | 1982-03-19 | Grace W R Ltd | PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING IMMOBILIZED MICROORGANISMS, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR IMMOBILIZING THEM AND METHOD FOR THE PREPARATION THEREOF |
WO2001090203A1 (en) * | 2000-05-25 | 2001-11-29 | Institut Francais De Recherche Pour L'exploitation De Mer (Ifremer) | Insoluble random copolymers having a specific affinity for a given protist, uses thereof and method for selecting them |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2352827A1 (en) * | 1976-05-26 | 1977-12-23 | Rhone Poulenc Ind | SUPPORT-AMINOACYLASE COMPLEXES |
-
1966
- 1966-03-21 GB GB12399/66A patent/GB1108533A/en not_active Expired
- 1966-03-21 DE DE19661517761 patent/DE1517761A1/en active Pending
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2059566A1 (en) * | 1969-07-28 | 1971-06-04 | Cancer Res Center | |
FR2073443A1 (en) * | 1969-12-02 | 1971-10-01 | Baxter Laboratories Inc | |
FR2490241A1 (en) * | 1980-09-17 | 1982-03-19 | Grace W R Ltd | PROCESS FOR THE PREPARATION OF L-ASPARTIC ACID, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR USE AND METHOD FOR THE PREPARATION THEREOF |
FR2490240A1 (en) * | 1980-09-17 | 1982-03-19 | Grace W R Ltd | PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING IMMOBILIZED MICROORGANISMS, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR IMMOBILIZING THEM AND METHOD FOR THE PREPARATION THEREOF |
WO2001090203A1 (en) * | 2000-05-25 | 2001-11-29 | Institut Francais De Recherche Pour L'exploitation De Mer (Ifremer) | Insoluble random copolymers having a specific affinity for a given protist, uses thereof and method for selecting them |
FR2809405A1 (en) * | 2000-05-25 | 2001-11-30 | Ifremer | INSOLUBLE STATISTIC COPOLYMERS HAVING SPECIFIC AFFINITY TO A GIVEN PROTIST, USES THEREOF AND METHOD OF SELECTING THEM |
Also Published As
Publication number | Publication date |
---|---|
DE1517761A1 (en) | 1970-01-29 |
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