GB1108533A - Water-insoluble enzymes - Google Patents

Water-insoluble enzymes

Info

Publication number
GB1108533A
GB1108533A GB12399/66A GB1239966A GB1108533A GB 1108533 A GB1108533 A GB 1108533A GB 12399/66 A GB12399/66 A GB 12399/66A GB 1239966 A GB1239966 A GB 1239966A GB 1108533 A GB1108533 A GB 1108533A
Authority
GB
United Kingdom
Prior art keywords
acid
enzyme
amino
macromolecular compound
norleucine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB12399/66A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of GB1108533A publication Critical patent/GB1108533A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B11/00—Preparation of cellulose ethers
    • C08B11/02—Alkyl or cycloalkyl ethers
    • C08B11/04—Alkyl or cycloalkyl ethers with substituted hydrocarbon radicals
    • C08B11/14—Alkyl or cycloalkyl ethers with substituted hydrocarbon radicals with nitrogen-containing groups
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F8/00—Chemical modification by after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A water-insoluble enzyme comprises the reaction product of a bacterial acylase and a macromolecular compound having a functional group capable of coupling with an enzyme protein without substantially decreasing the activity thereof. The enzyme may be produced by cultivation of Micrococcus glutamicus or Pseudomonas cruciviae. The macromolecular compound may be polyaminostyrene, p-amino-benzyl cellulose, a nitrated copolymer of methacrylic acid and methacrylic acid-3-fluoroanilide or any compound containing aminophenyl, acid anhydride, acid chloride, isocyanate, acid azide or nitro-fluorophenyl groups.ALSO:Racemic amino acids are resolved by acylating the amino group and selectively hydrolysing the acyl group of the L-form with an enzyme which is rendered insoluble by reaction with a macromolecular compound. Acylated amino acids which may be so hydrolysed includ N-acetyl - L - methionine, - L - alanine, - L - valine, - L - leucine, - L - threonine, - L - norvaline and - L - norleucine, and N - chloroacetyl - L - isoleucine, - L - norleucine, - L - serine, - L - alanine, - L - phenylalanine and - L - valine. Other acid groups which may be removed are benzoyl, dichloroacetyl, chloropropionyl and formyl. The O-form remains unattacked and may be recovered and hydrolysed with acid to give the O-form of the amino acid.
GB12399/66A 1965-03-20 1966-03-21 Water-insoluble enzymes Expired GB1108533A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1592965 1965-03-20

Publications (1)

Publication Number Publication Date
GB1108533A true GB1108533A (en) 1968-04-03

Family

ID=11902447

Family Applications (1)

Application Number Title Priority Date Filing Date
GB12399/66A Expired GB1108533A (en) 1965-03-20 1966-03-21 Water-insoluble enzymes

Country Status (2)

Country Link
DE (1) DE1517761A1 (en)
GB (1) GB1108533A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2059566A1 (en) * 1969-07-28 1971-06-04 Cancer Res Center
FR2073443A1 (en) * 1969-12-02 1971-10-01 Baxter Laboratories Inc
FR2490241A1 (en) * 1980-09-17 1982-03-19 Grace W R Ltd PROCESS FOR THE PREPARATION OF L-ASPARTIC ACID, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR USE AND METHOD FOR THE PREPARATION THEREOF
FR2490240A1 (en) * 1980-09-17 1982-03-19 Grace W R Ltd PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING IMMOBILIZED MICROORGANISMS, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR IMMOBILIZING THEM AND METHOD FOR THE PREPARATION THEREOF
WO2001090203A1 (en) * 2000-05-25 2001-11-29 Institut Francais De Recherche Pour L'exploitation De Mer (Ifremer) Insoluble random copolymers having a specific affinity for a given protist, uses thereof and method for selecting them

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2352827A1 (en) * 1976-05-26 1977-12-23 Rhone Poulenc Ind SUPPORT-AMINOACYLASE COMPLEXES

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2059566A1 (en) * 1969-07-28 1971-06-04 Cancer Res Center
FR2073443A1 (en) * 1969-12-02 1971-10-01 Baxter Laboratories Inc
FR2490241A1 (en) * 1980-09-17 1982-03-19 Grace W R Ltd PROCESS FOR THE PREPARATION OF L-ASPARTIC ACID, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR USE AND METHOD FOR THE PREPARATION THEREOF
FR2490240A1 (en) * 1980-09-17 1982-03-19 Grace W R Ltd PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING IMMOBILIZED MICROORGANISMS, HYDROPHILIC POLYETHER POLYURETHANE FOAM FOR IMMOBILIZING THEM AND METHOD FOR THE PREPARATION THEREOF
WO2001090203A1 (en) * 2000-05-25 2001-11-29 Institut Francais De Recherche Pour L'exploitation De Mer (Ifremer) Insoluble random copolymers having a specific affinity for a given protist, uses thereof and method for selecting them
FR2809405A1 (en) * 2000-05-25 2001-11-30 Ifremer INSOLUBLE STATISTIC COPOLYMERS HAVING SPECIFIC AFFINITY TO A GIVEN PROTIST, USES THEREOF AND METHOD OF SELECTING THEM

Also Published As

Publication number Publication date
DE1517761A1 (en) 1970-01-29

Similar Documents

Publication Publication Date Title
Inoue et al. Enzymatic deacetylation of histone
FUKUMOTO et al. Studies on lipase IV. Purification and properties of a lipase secreted by Rhizopus delemar
HUP9901198A2 (en) Process for producing l-amino acids
US3620925A (en) Process for purifying l-asparaginase
LV10727A (en) Potato alpha-amylase gens
Cole Properties of the penicillin deacylase enzyme of Escherichia coli
ATE141952T1 (en) ENZYME CATALYSED PROCESS FOR PRODUCING OPTICALLY ACTIVE CARBOXIC ACIDS
GB1108533A (en) Water-insoluble enzymes
Kaufmann et al. Variety of substrates for a bacterial benzyl penicillin-splitting enzyme
SE7607907L (en) ENZYME COMPLEX FOR THE CONVERSION OF RACEMIC HYDANTOINS TO OPTICALLY ACTIVE AMINO ACIDS
GB1414634A (en) Processes for the enzymatic hydrolysis of protein and skin rag
GB1409002A (en) Culture medium for bacteria
ATE1612T1 (en) STEREOSELECTIVE CLEAVAGE OF PHENYLGLYCINE DERIVATIVES WITH ENZYME RESINS.
DE3665408D1 (en) Process for reprocessing the solution which is left after the removal of the enzymes upon the enzymatic racematic splitting of an n-acetyl-dl-amino acid in the presence of an l-amino acid acylase
GB1369462A (en) Enzymic resolution of racemic n-acyl-dl-amino acids
GB1407488A (en) Process for the enzymatic depilation of animal skins
FR2188608A5 (en) Penicillins prepn by acylating 6-apa - in presence of catalytic enzymes using free acids
IE44288L (en) Preparation of amino acid mixture.
SU731935A1 (en) Method for destructing cell membrane of chlorella
GB1111738A (en) Improvement in interferon production
DE68915568D1 (en) Process for obtaining the protease from Endothia parasitica.
GB1029978A (en) Improvements in or relating to the production of proteolytic enzymes
BG89739A (en) METHOD FOR INCREASE ENZYME ACTIVITY AND SYNTHETIC PRODUCTIVITY OF ORGANISMS
GB1293563A (en) Enzymes and methods for their preparation, isolation and purification
GB1349498A (en) Water-insoluble bound enzyme