GB1105136A - Vaccines comprising living protozoa of the genus theileria - Google Patents
Vaccines comprising living protozoa of the genus theileriaInfo
- Publication number
- GB1105136A GB1105136A GB1263964A GB1263964A GB1105136A GB 1105136 A GB1105136 A GB 1105136A GB 1263964 A GB1263964 A GB 1263964A GB 1263964 A GB1263964 A GB 1263964A GB 1105136 A GB1105136 A GB 1105136A
- Authority
- GB
- United Kingdom
- Prior art keywords
- culture
- protozoa
- mixed
- cell line
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/015—Hemosporidia antigens, e.g. Plasmodium antigens
- A61K39/018—Babesia antigens, e.g. Theileria antigens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
-
- D—TEXTILES; PAPER
- D02—YARNS; MECHANICAL FINISHING OF YARNS OR ROPES; WARPING OR BEAMING
- D02G—CRIMPING OR CURLING FIBRES, FILAMENTS, THREADS, OR YARNS; YARNS OR THREADS
- D02G1/00—Producing crimped or curled fibres, filaments, yarns, or threads, giving them latent characteristics
- D02G1/12—Producing crimped or curled fibres, filaments, yarns, or threads, giving them latent characteristics using stuffer boxes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Mechanical Engineering (AREA)
- Textile Engineering (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
A method for the propagation of protozoa of the genus Theileria comprises the propagation of host cells containing the agamonts of the protozoa in association with a concurrent propagation of a tissue culture cell line not infectible with the protozoa, as a mixed culture carried out in an aqueous nutrient medium. The tissue culture cell line may be the baby hamster kidney cell line BHK 21. The host cells may be blood leucocytes from an infected calf in the case of T. annulata or cells from the spleen or lymph nodes of an infected steer in the case of T. parva. A typical culture medium comprises a mixture of borine serum, tryptose phosphate broth and Eagle's basal medium, preferably with an increased aminoacid and vitamin content. The mixed culture may be sub-cultured by the monolayer culture technique. The mixed culture may be administered to cattle to render them immune to theileriasis.ALSO:A vaccine comprises living protozoa of the germs Theileria which have been propagated in host cells concurrently with a tissue culture cell line not infectible with the protozoa as a mixed culture in an aqueous nutrient medium. The host cells may be blood leucocytes from an infected calf in the case of T. annulata or cells from the spleen of lymph nodes of an infected steer in the case of T. parva. The tissue culture cell line may be baby hamster kidney cells BHK 21, which have been trypsinised. The culture medium contains the usual nutrients required in the p propagation of animal cells, e.g. it may comprise a mixture of bovine serum, tryptose phosphate broth and Eagle's basal medium, preferably with an increased amino-acid and vitamin content. The mixed culture may be sub-cultured by the monolayer culture technique. The protozoa is propagated by incubation of the mixed culture at 37-40 DEG C. and by successive transfers of the propagated mixed cell culture into a new culture medium, e.g. every third or fourth day. The mixed tissue culture obtained by sub-culturing may be centrifuged and resuspended. The suspension may be used as a vaccine for administration to cattle to render them immune to theileriasis.
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1263964A GB1105136A (en) | 1964-03-25 | 1964-03-25 | Vaccines comprising living protozoa of the genus theileria |
| IL2318965A IL23189A (en) | 1964-03-25 | 1965-03-21 | Propagation of protozoa |
| IT630165A IT965510B (en) | 1964-03-25 | 1965-03-24 | METHOD FOR THE PROPAGATION OF PROTOZOA OF THE GENE THEILERIA FOR THE PREPARATION OF VETERINARY VACCINES |
| DEP1270A DE1270222B (en) | 1964-03-25 | 1965-03-24 | Method for the propagation of protozoa of the genus Theileria |
| CH417065A CH472497A (en) | 1964-03-25 | 1965-03-25 | Method for the propagation of protozoa of the genus Theileria |
| NL6503796A NL6503796A (en) | 1964-03-25 | 1965-03-25 | |
| FR1594510D FR1594510A (en) | 1964-03-25 | 1965-03-25 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1263964A GB1105136A (en) | 1964-03-25 | 1964-03-25 | Vaccines comprising living protozoa of the genus theileria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1105136A true GB1105136A (en) | 1968-03-06 |
Family
ID=10008410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1263964A Expired GB1105136A (en) | 1964-03-25 | 1964-03-25 | Vaccines comprising living protozoa of the genus theileria |
Country Status (7)
| Country | Link |
|---|---|
| CH (1) | CH472497A (en) |
| DE (1) | DE1270222B (en) |
| FR (1) | FR1594510A (en) |
| GB (1) | GB1105136A (en) |
| IL (1) | IL23189A (en) |
| IT (1) | IT965510B (en) |
| NL (1) | NL6503796A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998014212A1 (en) * | 1996-09-30 | 1998-04-09 | Embrex, Inc. | Method of producing active immunity with vaccine conjugate |
-
1964
- 1964-03-25 GB GB1263964A patent/GB1105136A/en not_active Expired
-
1965
- 1965-03-21 IL IL2318965A patent/IL23189A/en unknown
- 1965-03-24 IT IT630165A patent/IT965510B/en active
- 1965-03-24 DE DEP1270A patent/DE1270222B/en active Pending
- 1965-03-25 CH CH417065A patent/CH472497A/en not_active IP Right Cessation
- 1965-03-25 NL NL6503796A patent/NL6503796A/xx unknown
- 1965-03-25 FR FR1594510D patent/FR1594510A/fr not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998014212A1 (en) * | 1996-09-30 | 1998-04-09 | Embrex, Inc. | Method of producing active immunity with vaccine conjugate |
| US6440408B2 (en) | 1996-09-30 | 2002-08-27 | University Of Arkansas | Method of treatment |
| US6890527B2 (en) | 1996-09-30 | 2005-05-10 | The University Of Arkansas | Methods of treatment |
| CN100389827C (en) * | 1996-09-30 | 2008-05-28 | 阿肯萨斯大学 | Method of producing active immunity with vaccine conjugate |
Also Published As
| Publication number | Publication date |
|---|---|
| IL23189A (en) | 1969-01-29 |
| IT965510B (en) | 1974-02-11 |
| DE1270222B (en) | 1968-06-12 |
| NL6503796A (en) | 1965-09-27 |
| CH472497A (en) | 1969-05-15 |
| FR1594510A (en) | 1970-06-08 |
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