GB1086478A - Anti-protein to human chorionic gonadotrophin - Google Patents
Anti-protein to human chorionic gonadotrophinInfo
- Publication number
- GB1086478A GB1086478A GB4451865A GB4451865A GB1086478A GB 1086478 A GB1086478 A GB 1086478A GB 4451865 A GB4451865 A GB 4451865A GB 4451865 A GB4451865 A GB 4451865A GB 1086478 A GB1086478 A GB 1086478A
- Authority
- GB
- United Kingdom
- Prior art keywords
- protein
- hcg
- serum
- particles
- animals
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/44—Antibodies bound to carriers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Endocrinology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Public Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Reproductive Health (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A diagnostic reagent for pregnancy determination similar to that of Specification 1,078,939 contains an anti protein for human chorionic gonadotrophin, (HCG), derived from the blood serum of animals rendered immune to HCG by repeated injections thereof, and extracted by adsorbing the anti-protein on water swollen particles of cross-linked dextran containing random ether linkages, such as Sephadex G; (Registered Trade Mark), and then eluting the anti protein. (see Div. A5-A6). For use the anti-protein is adsorbed on polystyrene latex particles which preferably have an average diameter of from 0.5 to 1.3 microns. The reagent consists of the anti-protein coated polystyrene particles in suspension in an aqueous solution containing 0.45% to 1.5% glycine, 0.2% to 0.45% of the sodium salt of EDTA, 4% to 6% albumin, 0.05% to 0.1% sodium azide, and triethanolamine to adjust the pH to from 8.2 to 8.6.ALSO:A specific anti-protein to human chorionic gonadotrophin (HCG) is isolated from the blood serum of a lower animal containing the anti-protein by adsorbing the serum on water-swollen particles of cross-linked dextran containing random ether linkages to glucose residues in the polysaccharide chains (e.g. Sephadex G; Registered Trade Mark), eluting the anti-protein, and recovering it from the eluate. Elution is suitably effected with an aqueous solution of sodium chloride and tris (hydroxymethyl)-aminomethane of pH 8.0, the same reagent being used to swell the dextran gel. As a serum source, animals such as rabbits may be immunised with a number of injections of a commercial human HCG preparation, the animals being bled when a suitable anti-serum titre has been reached. Recovery of the anti-protein from the eluate is by lyophilisation, re-solution and dialysis. The HCG anti-protein is of use in preparing a pregnancy diagnostic reagent, by adsorption on to polystyrene latex particles (see Div. B1-B2)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38917764A | 1964-08-12 | 1964-08-12 | |
US47899965A | 1965-08-11 | 1965-08-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1086478A true GB1086478A (en) | 1967-10-11 |
Family
ID=27012580
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB5168864A Expired GB1078939A (en) | 1964-08-12 | 1964-12-18 | Diagnostic reagent |
GB4451865A Expired GB1086478A (en) | 1964-08-12 | 1965-10-20 | Anti-protein to human chorionic gonadotrophin |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB5168864A Expired GB1078939A (en) | 1964-08-12 | 1964-12-18 | Diagnostic reagent |
Country Status (2)
Country | Link |
---|---|
BE (1) | BE684708A (en) |
GB (2) | GB1078939A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2459480A1 (en) * | 1979-06-19 | 1981-01-09 | Siber George | AGGLUTINATION DIAGNOSTIC TESTS AND REAGENTS AND METHODS FOR PREPARING SUCH REAGENTS |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4003988A (en) * | 1976-06-01 | 1977-01-18 | Warner-Lambert Company | Direct agglutination test for pregnancy |
JPS5341420A (en) * | 1976-09-29 | 1978-04-14 | Mochida Pharm Co Ltd | Immunochemically measuring methoa of hapten |
-
1964
- 1964-12-18 GB GB5168864A patent/GB1078939A/en not_active Expired
-
1965
- 1965-10-20 GB GB4451865A patent/GB1086478A/en not_active Expired
-
1966
- 1966-07-28 BE BE684708D patent/BE684708A/xx unknown
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2459480A1 (en) * | 1979-06-19 | 1981-01-09 | Siber George | AGGLUTINATION DIAGNOSTIC TESTS AND REAGENTS AND METHODS FOR PREPARING SUCH REAGENTS |
Also Published As
Publication number | Publication date |
---|---|
BE684708A (en) | 1967-01-30 |
GB1078939A (en) | 1967-08-09 |
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