GB1073767A - Enzymatic analysis - Google Patents

Enzymatic analysis

Info

Publication number
GB1073767A
GB1073767A GB21475/66A GB2147566A GB1073767A GB 1073767 A GB1073767 A GB 1073767A GB 21475/66 A GB21475/66 A GB 21475/66A GB 2147566 A GB2147566 A GB 2147566A GB 1073767 A GB1073767 A GB 1073767A
Authority
GB
United Kingdom
Prior art keywords
meter
sample
timer
gain control
balancing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB21475/66A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Laboratory Equipment Corp
Original Assignee
Laboratory Equipment Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Laboratory Equipment Corp filed Critical Laboratory Equipment Corp
Publication of GB1073767A publication Critical patent/GB1073767A/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/021—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance before and after chemical transformation of the material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Emergency Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

1,073,767. Electrophysical measurement, control &c. LABORATORY EQUIPMENT CORPORATION. May 13, 1966 [May 14, 1965], No. 21475/66. Heading G1N. The invention relates to the determination of e.g. urea or glucose in a blood sample, by measuring the variation in conductivity at a predetermined time during an enzyme reaction. The conductivity cell 38, (comprising electrodes lowered into a beaker, ) is connected in a differential circuit with a balancing resistor 78 across the secondary of a transformer 68. The output is fed through an amplifier and rectifier to a meter M. An amplifier gain control 88 is ganged to the balancing resistor, and a "calibrating" resistor 84 is provided. Supply circuits for a stirrer motor 114, timer 14, and meter clamping device 140 are shown. Calibration:- urease is added to a standard serum containing a known urea nitrogen content, and the timer is started. For the first 20 seconds the ganged balancing/gain control is turned to keep the meter at zero. Then for the next 60 seconds the "calibration" gain control is used to maintain the meter deflection at the known percentage content of the sample until the meter clamp is operated by the timer. Measurement of urea content is made after calibration by mixing urease with the sample, starting the timer and maintaining the meter at zero for 20 seconds by the balancing/gain control as before. The meter is then allowed to deflect until the clamp operates, when a direct reading of the urea content is obtained. Modification; for the measurement of glucose in a sample (which is decomposed by glucose oxidase to gluconic acid) the sample is first deionized by passing through an ion exchange resin "filter" to remove other normally present conductive ions which might lead to inaccurate results.
GB21475/66A 1965-05-14 1966-05-13 Enzymatic analysis Expired GB1073767A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US45582865A 1965-05-14 1965-05-14

Publications (1)

Publication Number Publication Date
GB1073767A true GB1073767A (en) 1967-06-28

Family

ID=23810434

Family Applications (1)

Application Number Title Priority Date Filing Date
GB21475/66A Expired GB1073767A (en) 1965-05-14 1966-05-13 Enzymatic analysis

Country Status (2)

Country Link
US (1) US3421982A (en)
GB (1) GB1073767A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2406825A1 (en) * 1977-10-22 1979-05-18 Mitsubishi Chem Ind METHOD AND APPARATUS FOR THE DETERMINATION OF GLUCOSE

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3506405A (en) * 1965-08-27 1970-04-14 Precision Scient Co Automatic titration instrument
US3857771A (en) * 1967-02-27 1974-12-31 Beckman Instruments Inc Rate sensing batch analyzer
US3605010A (en) * 1969-07-14 1971-09-14 Becton Dickinson Co Automatic lysis detection apparatus
US3746984A (en) * 1970-09-08 1973-07-17 Triplett Corp Autopolarity voltmeter circuit
US3933593A (en) * 1971-02-22 1976-01-20 Beckman Instruments, Inc. Rate sensing batch analysis method
US4045296A (en) * 1972-08-09 1977-08-30 Beckman Instruments, Inc. Rate sensing batch analysis method and enzyme used therein
JPS518189U (en) * 1974-07-04 1976-01-21
US3957592A (en) * 1974-07-29 1976-05-18 Owens-Illinois, Inc. Measurement of polarographic current
US4271001A (en) * 1978-03-23 1981-06-02 Asahi Kasei Kogyo Kabushiki Kaisha Apparatus for measuring membrane characteristics of vesicles
US4204037A (en) * 1978-04-04 1980-05-20 Nasa Method and automated apparatus for detecting coliform organisms
JPS55104757A (en) * 1979-02-07 1980-08-11 Ootake Seisakusho:Kk Device for automatically measuring extinction of gas
US4350579A (en) * 1980-09-04 1982-09-21 Applied Medical Technology, Inc. Testing apparatus for measuring ions in fluids
US4560534A (en) * 1983-11-02 1985-12-24 Miles Laboratories, Inc. Polymer catalyst transducers
US4642559A (en) * 1983-12-12 1987-02-10 Texaco, Inc. Electrostatic field meter
US4672322A (en) * 1984-08-24 1987-06-09 Milton Roy Company High sensitivity conductivity detector
US5120648A (en) * 1988-05-26 1992-06-09 Lim Technology Laboratories, Inc. Chemical analyzer using rf radiation attenuation measurements

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2406825A1 (en) * 1977-10-22 1979-05-18 Mitsubishi Chem Ind METHOD AND APPARATUS FOR THE DETERMINATION OF GLUCOSE

Also Published As

Publication number Publication date
US3421982A (en) 1969-01-14

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