GB1058828A - Process for the manufacture of artificial antigens - Google Patents

Process for the manufacture of artificial antigens

Info

Publication number
GB1058828A
GB1058828A GB3885563A GB3885563A GB1058828A GB 1058828 A GB1058828 A GB 1058828A GB 3885563 A GB3885563 A GB 3885563A GB 3885563 A GB3885563 A GB 3885563A GB 1058828 A GB1058828 A GB 1058828A
Authority
GB
United Kingdom
Prior art keywords
protein
diisocyanate
linking
albumin
component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3885563A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of GB1058828A publication Critical patent/GB1058828A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Artificial antigens are produced by linking a carrier protein via an organic bridge group to a serologically determinant peptide, e.g. a protein hormone, toxin, enzyme or a virus or bacterium having a surface component with protein characteristics. The linkage may be formed by a chemical process such as forming a diazo or azide derivative of one component which is coupled to the phenolic hydroxyl or amine groups of the other or by cross-linking the two components by reaction with a diisocyanate. The specific example relates to a product obtained by formation of a diazo group-containing derivative of a C-terminal hexapeptide obtained by enzymatic digestion of tobacco mosaic virus and coupling with a diisocyanate-cross -linked cattle albumin. Suitable carrier proteins are egg albumin, serum albumin, lactalbumin, g -globulins and gelatin and its derivatives.ALSO:Artificial antigens are produced by linking a "carrier protein" via an organic bridge group to a serologically determinant peptide. Suitable carrier proteins are: egg albumin, lacta bumin, serum albumin, g -globulins and gelatin and its derivatives. The serologically determinant peptide may be a protein hormone, toxin, enzyme or a virus or bacterium whose surface component has protein characteristics. The linkage may be formed by a chemical process such as (1) forming a diazo derivative of one component, which is coupled to a phenolic hydroxyl group of the other, (2) by similarly forming an azide of one component, which is coupled to the amino group of the other or (3) by cross-linking the two components by reaction with a diisocyanate. The specific example relates to a product obtained by formation of a diazo derivative of a C-terminal hexapeptide obtained by enzymatic digestion of the tobacco mosaic virus and coupling with a diisocyanate cross-linked cattle albumin.
GB3885563A 1963-07-17 1963-10-02 Process for the manufacture of artificial antigens Expired GB1058828A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEB0072720 1963-07-17

Publications (1)

Publication Number Publication Date
GB1058828A true GB1058828A (en) 1967-02-15

Family

ID=6977565

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3885563A Expired GB1058828A (en) 1963-07-17 1963-10-02 Process for the manufacture of artificial antigens

Country Status (2)

Country Link
DE (1) DE1467782A1 (en)
GB (1) GB1058828A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2421943A1 (en) * 1973-05-07 1974-11-28 Univ Ohio MODIFIED POLYPEPTIDES
DE2646223A1 (en) * 1975-10-14 1977-04-28 Univ Ohio NEW IMPROVED ORGANIC COMPOUNDS
EP0094844A2 (en) * 1982-05-18 1983-11-23 The Regents Of The University Of California Drug-carrier conjugates
EP0095426A1 (en) * 1982-05-26 1983-11-30 CNRS, Centre National de la Recherche Scientifique Synthetic polypeptides containing at least one sequence of the choleric B1 sub-unit toxin, and medicines containing them
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
US6146633A (en) * 1973-05-07 2000-11-14 The Ohio State University Method for treatment of antigenically modified polypeptides
CN104383889A (en) * 2014-11-21 2015-03-04 中国农业科学院油料作物研究所 Dihydrocapsaicin polyclonal antibody immunoadsorbent, immunoaffinity column and preparation method and application thereof
CN104447383A (en) * 2014-11-21 2015-03-25 中国农业科学院油料作物研究所 Dihydrocapsaicin artificial hapten and artificial antigen as well as preparation methods thereof

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2421943A1 (en) * 1973-05-07 1974-11-28 Univ Ohio MODIFIED POLYPEPTIDES
US6146633A (en) * 1973-05-07 2000-11-14 The Ohio State University Method for treatment of antigenically modified polypeptides
DE2646223A1 (en) * 1975-10-14 1977-04-28 Univ Ohio NEW IMPROVED ORGANIC COMPOUNDS
EP0094844A3 (en) * 1982-05-18 1984-11-21 The Regents Of The University Of California Drug-carrier conjugates
EP0094844A2 (en) * 1982-05-18 1983-11-23 The Regents Of The University Of California Drug-carrier conjugates
US4587046A (en) * 1982-05-18 1986-05-06 The Regents Of The University Of California Drug-carrier conjugates
EP0095426A1 (en) * 1982-05-26 1983-11-30 CNRS, Centre National de la Recherche Scientifique Synthetic polypeptides containing at least one sequence of the choleric B1 sub-unit toxin, and medicines containing them
FR2527445A1 (en) * 1982-05-26 1983-12-02 Centre Nat Rech Scient MEDICINAL PRODUCTS CONTAINING AT LEAST ONE SEQUENCE OF THE CHOLERIC TOXIN B1 SUBUNIT
FR2532850A1 (en) * 1982-09-15 1984-03-16 Pasteur Institut Immunogenic conjugates between a hapten and a carrier molecule derived from a toxin, vaccines comprising them and method for obtaining them
CN104383889A (en) * 2014-11-21 2015-03-04 中国农业科学院油料作物研究所 Dihydrocapsaicin polyclonal antibody immunoadsorbent, immunoaffinity column and preparation method and application thereof
CN104447383A (en) * 2014-11-21 2015-03-25 中国农业科学院油料作物研究所 Dihydrocapsaicin artificial hapten and artificial antigen as well as preparation methods thereof
CN104447383B (en) * 2014-11-21 2016-03-23 中国农业科学院油料作物研究所 A kind of Dihydrocapsaicin artificial semiantigen, artificial antigen and preparation method thereof
CN104383889B (en) * 2014-11-21 2016-08-24 中国农业科学院油料作物研究所 A kind of Dihydrocapsaicin polyclonal antibody adsorbent, immune affinity column and its preparation method and application

Also Published As

Publication number Publication date
DE1467782A1 (en) 1968-12-12

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