GB0416023D0 - Method - Google Patents

Method

Info

Publication number
GB0416023D0
GB0416023D0 GBGB0416023.0A GB0416023A GB0416023D0 GB 0416023 D0 GB0416023 D0 GB 0416023D0 GB 0416023 A GB0416023 A GB 0416023A GB 0416023 D0 GB0416023 D0 GB 0416023D0
Authority
GB
United Kingdom
Prior art keywords
enzyme
variant
amino acid
optionally
acyltransferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB0416023.0A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DuPont Nutrition Biosciences ApS
Danisco US Inc
Original Assignee
Danisco AS
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IB2004/000655 external-priority patent/WO2004064537A2/en
Application filed by Danisco AS, Danisco US Inc filed Critical Danisco AS
Priority to US10/898,775 priority Critical patent/US7718408B2/en
Publication of GB0416023D0 publication Critical patent/GB0416023D0/en
Priority to CNA2004800386995A priority patent/CN1898386A/en
Priority to PCT/IB2004/004374 priority patent/WO2005066351A2/en
Priority to DK04806534T priority patent/DK1704240T3/en
Priority to JP2006546408A priority patent/JP4949042B2/en
Priority to DE602004009713T priority patent/DE602004009713T2/en
Priority to ES04806534T priority patent/ES2294575T3/en
Priority to EP07017066A priority patent/EP1862554B1/en
Priority to DE602004026229T priority patent/DE602004026229D1/en
Priority to EP04806534A priority patent/EP1704240B1/en
Priority to AU2004312213A priority patent/AU2004312213B2/en
Priority to AT07017066T priority patent/ATE462011T1/en
Priority to NZ547082A priority patent/NZ547082A/en
Priority to BRPI0418107-7A priority patent/BRPI0418107A/en
Priority to AT04806534T priority patent/ATE376593T1/en
Priority to CA2550789A priority patent/CA2550789C/en
Priority to CNA2004800389334A priority patent/CN1898391A/en
Priority to HK06113532A priority patent/HK1091868A1/en
Priority to HK08100607.7A priority patent/HK1106953A1/en
Priority to US12/492,042 priority patent/US8440435B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)

Abstract

The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
GBGB0416023.0A 2003-12-24 2004-07-16 Method Ceased GB0416023D0 (en)

Priority Applications (20)

Application Number Priority Date Filing Date Title
US10/898,775 US7718408B2 (en) 2003-12-24 2004-07-26 Method
CA2550789A CA2550789C (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
CNA2004800389334A CN1898391A (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
EP04806534A EP1704240B1 (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
AU2004312213A AU2004312213B2 (en) 2003-12-24 2004-12-23 Enzymatic Treatment of Oils
DK04806534T DK1704240T3 (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
JP2006546408A JP4949042B2 (en) 2003-12-24 2004-12-23 Enzymatic treatment of cooking oil
DE602004009713T DE602004009713T2 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS
ES04806534T ES2294575T3 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS.
EP07017066A EP1862554B1 (en) 2003-12-24 2004-12-23 Enzymatic removal of diglycerides from edible oils
DE602004026229T DE602004026229D1 (en) 2003-12-24 2004-12-23 ENZYMATIC REMOVAL OF DIGLYCERIDES FROM EDIBLE OILS
CNA2004800386995A CN1898386A (en) 2003-12-24 2004-12-23 Proteins
PCT/IB2004/004374 WO2005066351A2 (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
AT07017066T ATE462011T1 (en) 2003-12-24 2004-12-23 ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS
NZ547082A NZ547082A (en) 2003-12-24 2004-12-23 Enzymatic treatment of oils
BRPI0418107-7A BRPI0418107A (en) 2003-12-24 2004-12-23 enzymatic treatment of oils
AT04806534T ATE376593T1 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS
HK06113532A HK1091868A1 (en) 2003-12-24 2006-12-08 Enzymatic treatment of oils
HK08100607.7A HK1106953A1 (en) 2003-12-24 2008-01-17 Enzymatic removal of diglycerides from edible oils
US12/492,042 US8440435B2 (en) 2003-12-24 2009-06-25 Method for reducing 1,2-diglyceride content of an edible oil

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0330016.7A GB0330016D0 (en) 2003-12-24 2003-12-24 Method
PCT/IB2004/000655 WO2004064537A2 (en) 2003-01-17 2004-01-15 Method for the in situ production of an emulsifier in foodstuff

Publications (1)

Publication Number Publication Date
GB0416023D0 true GB0416023D0 (en) 2004-08-18

Family

ID=30776521

Family Applications (3)

Application Number Title Priority Date Filing Date
GBGB0330016.7A Ceased GB0330016D0 (en) 2003-01-14 2003-12-24 Method
GBGB0415999.2A Ceased GB0415999D0 (en) 2003-12-24 2004-07-16 Proteins
GBGB0416023.0A Ceased GB0416023D0 (en) 2003-12-24 2004-07-16 Method

Family Applications Before (2)

Application Number Title Priority Date Filing Date
GBGB0330016.7A Ceased GB0330016D0 (en) 2003-01-14 2003-12-24 Method
GBGB0415999.2A Ceased GB0415999D0 (en) 2003-12-24 2004-07-16 Proteins

Country Status (10)

Country Link
JP (1) JP2010142243A (en)
CN (2) CN101676390A (en)
AT (1) ATE462011T1 (en)
DE (1) DE602004026229D1 (en)
DK (1) DK1704236T3 (en)
GB (3) GB0330016D0 (en)
HK (1) HK1106953A1 (en)
NZ (1) NZ547085A (en)
RU (2) RU2377300C2 (en)
ZA (1) ZA200605158B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114958800B (en) * 2022-06-24 2023-08-25 北京脉道生物药品制造有限公司 Taq DNA polymerase mutant capable of tolerating blood or blood product inhibition and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0977869B2 (en) * 1997-04-09 2008-11-12 Danisco A/S Lipase and use of same for improving doughs and baked products
WO2000005396A1 (en) * 1998-07-21 2000-02-03 Danisco A/S Foodstuff
DE50115482D1 (en) * 2001-07-11 2010-06-24 Cognis Ip Man Gmbh Lipase / acyltransferase from Candida parapsilosis
WO2005045016A2 (en) * 2003-08-11 2005-05-19 Codexis, Inc. Improved glucose dehydrogenase polypeptides and related polynucleotides

Also Published As

Publication number Publication date
RU2377307C2 (en) 2009-12-27
RU2006126654A (en) 2008-01-27
ATE462011T1 (en) 2010-04-15
DK1704236T3 (en) 2018-01-08
CN103275944A (en) 2013-09-04
DE602004026229D1 (en) 2010-05-06
RU2377300C2 (en) 2009-12-27
CN101676390A (en) 2010-03-24
HK1106953A1 (en) 2008-03-20
GB0330016D0 (en) 2004-01-28
ZA200605158B (en) 2008-04-30
RU2006126715A (en) 2008-01-27
JP2010142243A (en) 2010-07-01
GB0415999D0 (en) 2004-08-18
NZ547085A (en) 2009-11-27
CN103275944B (en) 2016-12-07

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)