GB0022981D0 - Manipulation of spermatozoa - Google Patents

Manipulation of spermatozoa

Info

Publication number
GB0022981D0
GB0022981D0 GB0022981A GB0022981A GB0022981D0 GB 0022981 D0 GB0022981 D0 GB 0022981D0 GB 0022981 A GB0022981 A GB 0022981A GB 0022981 A GB0022981 A GB 0022981A GB 0022981 D0 GB0022981 D0 GB 0022981D0
Authority
GB
United Kingdom
Prior art keywords
sperm
extracts
methods
nuclei
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GB0022981A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
St Georges Enterprises Ltd
Original Assignee
St Georges Enterprises Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St Georges Enterprises Ltd filed Critical St Georges Enterprises Ltd
Priority to GB0022981A priority Critical patent/GB0022981D0/en
Publication of GB0022981D0 publication Critical patent/GB0022981D0/en
Priority to AU2001287897A priority patent/AU2001287897A1/en
Priority to PCT/GB2001/004184 priority patent/WO2002024872A2/en
Ceased legal-status Critical Current

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a method of sperm permeabilisation comprising: (a) washing a sample containing sperm in a sperm nuclear extraction buffer (SNEB) which includes one or more metal chelating agents and which is substantially free of non-ionic detergents and divalent cations; (b) contacting the sperm with one or more permeabilising agents; and (c) optionally separating the sperm tail from the sperm head; and to a method of sperm transformation which method comprises incubating a sample of sperm or sperm nuclei in one or more cell-free extracts. Also described are extracts and buffers for use in these methods, methods of preparing the extracts and uses of the transformed sperm nuclei.
GB0022981A 2000-09-19 2000-09-19 Manipulation of spermatozoa Ceased GB0022981D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
GB0022981A GB0022981D0 (en) 2000-09-19 2000-09-19 Manipulation of spermatozoa
AU2001287897A AU2001287897A1 (en) 2000-09-19 2001-09-19 Manipulation of spermatozoa
PCT/GB2001/004184 WO2002024872A2 (en) 2000-09-19 2001-09-19 Manipulation of spermatozoa

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0022981A GB0022981D0 (en) 2000-09-19 2000-09-19 Manipulation of spermatozoa

Publications (1)

Publication Number Publication Date
GB0022981D0 true GB0022981D0 (en) 2000-11-01

Family

ID=9899743

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0022981A Ceased GB0022981D0 (en) 2000-09-19 2000-09-19 Manipulation of spermatozoa

Country Status (3)

Country Link
AU (1) AU2001287897A1 (en)
GB (1) GB0022981D0 (en)
WO (1) WO2002024872A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US7125976B2 (en) 2003-07-01 2006-10-24 Trustees Of Columbia University In The City Of New York Method of screening for agents inhibiting chloride intracellular channels

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480772A (en) * 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
GB9402586D0 (en) * 1994-02-10 1994-04-06 Hulten Maj Methods for chromosome condensation

Also Published As

Publication number Publication date
WO2002024872A2 (en) 2002-03-28
WO2002024872A3 (en) 2002-08-08
AU2001287897A1 (en) 2002-04-02
WO2002024872A8 (en) 2004-04-15

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)