GB0022981D0 - Manipulation of spermatozoa - Google Patents
Manipulation of spermatozoaInfo
- Publication number
- GB0022981D0 GB0022981D0 GB0022981A GB0022981A GB0022981D0 GB 0022981 D0 GB0022981 D0 GB 0022981D0 GB 0022981 A GB0022981 A GB 0022981A GB 0022981 A GB0022981 A GB 0022981A GB 0022981 D0 GB0022981 D0 GB 0022981D0
- Authority
- GB
- United Kingdom
- Prior art keywords
- sperm
- extracts
- methods
- nuclei
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Reproductive Health (AREA)
- Developmental Biology & Embryology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to a method of sperm permeabilisation comprising: (a) washing a sample containing sperm in a sperm nuclear extraction buffer (SNEB) which includes one or more metal chelating agents and which is substantially free of non-ionic detergents and divalent cations; (b) contacting the sperm with one or more permeabilising agents; and (c) optionally separating the sperm tail from the sperm head; and to a method of sperm transformation which method comprises incubating a sample of sperm or sperm nuclei in one or more cell-free extracts. Also described are extracts and buffers for use in these methods, methods of preparing the extracts and uses of the transformed sperm nuclei.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0022981A GB0022981D0 (en) | 2000-09-19 | 2000-09-19 | Manipulation of spermatozoa |
AU2001287897A AU2001287897A1 (en) | 2000-09-19 | 2001-09-19 | Manipulation of spermatozoa |
PCT/GB2001/004184 WO2002024872A2 (en) | 2000-09-19 | 2001-09-19 | Manipulation of spermatozoa |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0022981A GB0022981D0 (en) | 2000-09-19 | 2000-09-19 | Manipulation of spermatozoa |
Publications (1)
Publication Number | Publication Date |
---|---|
GB0022981D0 true GB0022981D0 (en) | 2000-11-01 |
Family
ID=9899743
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB0022981A Ceased GB0022981D0 (en) | 2000-09-19 | 2000-09-19 | Manipulation of spermatozoa |
Country Status (3)
Country | Link |
---|---|
AU (1) | AU2001287897A1 (en) |
GB (1) | GB0022981D0 (en) |
WO (1) | WO2002024872A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020142397A1 (en) | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
US7125976B2 (en) | 2003-07-01 | 2006-10-24 | Trustees Of Columbia University In The City Of New York | Method of screening for agents inhibiting chloride intracellular channels |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5480772A (en) * | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
GB9402586D0 (en) * | 1994-02-10 | 1994-04-06 | Hulten Maj | Methods for chromosome condensation |
-
2000
- 2000-09-19 GB GB0022981A patent/GB0022981D0/en not_active Ceased
-
2001
- 2001-09-19 WO PCT/GB2001/004184 patent/WO2002024872A2/en active Application Filing
- 2001-09-19 AU AU2001287897A patent/AU2001287897A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2002024872A2 (en) | 2002-03-28 |
WO2002024872A3 (en) | 2002-08-08 |
AU2001287897A1 (en) | 2002-04-02 |
WO2002024872A8 (en) | 2004-04-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
AT | Applications terminated before publication under section 16(1) |